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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 何佳安(Ja-an Annie Ho) | |
| dc.contributor.author | Shao-Ching Feng | en |
| dc.contributor.author | 馮劭擎 | zh_TW |
| dc.date.accessioned | 2021-06-07T23:54:50Z | - |
| dc.date.copyright | 2020-08-14 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-11 | |
| dc.identifier.citation | 1.Dole, M.; Wilson, F. R.; Fife, W. P., Hyperbaric Hydrogen Therapy - Possible Treatment for Cancer. Science 1975, 190 (4210), 152-154. 2.Ohsawa, I.; Ishikawa, M.; Takahashi, K.; Watanabe, M.; Nishimaki, K.; Yamagata, K.; Katsura, K.; Katayama, Y.; Asoh, S.; Ohta, S., Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med 2007, 13 (6), 688-694. 3.Ge, L.; Yang, M.; Yang, N. N.; Yin, X. X.; Song, W. G., Molecular hydrogen: a preventive and therapeutic medical gas for various diseases. Oncotarget 2017, 8 (60), 102653-102673. 4.Li, S.; Liao, R. R.; Sheng, X. Y.; Luo, X. J.; Zhang, X.; Wen, X. M.; Zhou, J.; Peng, K., Hydrogen Gas in Cancer Treatment. Front Oncol 2019, 9. 5.Yang, Q. X.; Ji, G. D.; Pan, R. T.; Zhao, Y. H.; Yan, P., Protective effect of hydrogen-rich water on liver function of colorectal cancer patients treated with mFOLFOX6 chemotherapy. Mol Clin Oncol 2017, 7 (5), 891-896. 6.Kang, K. M.; Kang, Y. N.; Choi, I. B.; Gu, Y.; Kawamura, T.; Toyoda, Y.; Nakao, A., Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors. Med Gas Res 2011, 1 (1), 11. 7.Kajiyama, S.; Hasegawa, G.; Asano, M.; Hosoda, H.; Fukui, M.; Nakamura, N.; Kitawaki, J.; Imai, S.; Nakano, K.; Ohta, M.; Adachi, T.; Obayashi, H.; Yoshikawa, T., Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance. Nutr Res 2008, 28 (3), 137-143. 8.Xia, C. X.; Liu, W. W.; Zeng, D. X.; Zhu, L. Y.; Sun, X. L.; Sun, X. J., Effect of Hydrogen-Rich Water on Oxidative Stress, Liver Function, and Viral Load in Patients with Chronic Hepatitis B. Cts-Clin Transl Sci 2013, 6 (5), 372-375. 9.Akagi, J.; Baba, H., Hydrogen gas restores exhausted CD8(+) T cells in patients with advanced colorectal cancer to improve prognosis. Oncol Rep 2019, 41 (1), 301-311. 10.Colin, D. J.; Limagne, E.; Ragot, K.; Lizard, G.; Ghiringhelli, F.; Solary, E.; Chauffert, B.; Latruffe, N.; Delmas, D., The role of reactive oxygen species and subsequent DNA-damage response in the emergence of resistance towards resveratrol in colon cancer models. Cell Death Dis 2014, 5. 11.Rodriguez-Vargas, J. M.; Ruiz-Magana, M. J.; Ruiz-Ruiz, C.; Majuelos-Melguizo, J.; Peralta-Leal, A.; Rodriguez, M. I.; Munoz-Gamez, J. A.; de Almodovar, M. R.; Siles, E.; Rivas, A. L.; Jaattela, M.; Oliver, F. J., ROS-induced DNA damage and PARP-1 are required for optimal induction of starvation-induced autophagy. Cell Res 2012, 22 (7), 1181-1198. 12.Cooke, M. S.; Evans, M. D.; Dizdaroglu, M.; Lunec, J., Oxidative DNA damage: mechanisms, mutation, and disease. Faseb J 2003, 17 (10), 1195-1214. 13.Jackson, S. P.; Bartek, J., The DNA-damage response in human biology and disease. Nature 2009, 461 (7267), 1071-1078. 14.Ohta, S., Molecular hydrogen as a preventive and therapeutic medical gas: initiation, development and potential of hydrogen medicine. Pharmacol Therapeut 2014, 144 (1), 1-11. 15.Dong, A. L.; Yu, Y.; Wang, Y. Y.; Li, C.; Chen, H. G.; Bian, Y. X.; Zhang, P.; Zhao, Y. G.; Yu, Y. H.; Xie, K. L., Protective effects of hydrogen gas against sepsis-induced acute lung injury via regulation of mitochondrial function and dynamics. Int Immunopharmacol 2018, 65, 366-372. 16.Zhou, P.; Lin, B.; Wang, P.; Pan, T.; Wang, S.; Chen, W. S.; Cheng, S. W.; Liu, S., The healing effect of hydrogen-rich water on acute radiation-induced skin injury in rats. J Radiat Res 2019, 60 (1), 17-22. 17.Iketani, M.; Ohshiro, J.; Urushibara, T.; Takahashi, M.; Arai, T.; Kawaguchi, H.; Ohsawa, I., Preadministration of Hydrogen-Rich Water Protects against Lipopolysaccharide-Induced Sepsis and Attenuates Liver Injury. Shock 2017, 48 (1), 85-93. 18.Zhang, N.; Deng, C. W.; Zhang, X. X.; Ning, Y. Y.; Zhang, J. X.; Bai, C., Inhalation of hydrogen gas attenuate airway inflammation and oxidative stress in allergic asthmatic mice. Eur Respir J 2017, 50. 19.Li, F. Y.; Zhu, S. X.; Wang, Z. P.; Wang, H.; Zhao, Y.; Chen, G. P., Consumption of hydrogen-rich water protects against ferric nitrilotriacetate-induced nephrotoxicity and early tumor promotional events in rats. Food Chem Toxicol 2013, 61, 248-254. 20.Gao, Y. A.; Yang, H. X.; Fan, Y. B.; Li, L.; Fang, J. H.; Yang, W., Hydrogen-Rich Saline Attenuates Cardiac and Hepatic Injury in Doxorubicin Rat Model by Inhibiting Inflammation and Apoptosis (vol 2016, 1320365, 2016). Mediat Inflamm 2017, 2017. 21.Yang, Y.; Liu, P. Y.; Bao, W.; Chen, S. J.; Wu, F. S.; Zhu, P. Y., Hydrogen inhibits endometrial cancer growth via a ROS/NLRP3/caspase-1/GSDMD-mediated pyroptotic pathway. Bmc Cancer 2020, 20 (1). 22.Fan, M. J.; Wen, Y. Y.; Ye, D.; Jin, Z. K.; Zhao, P. H.; Chen, D. Y.; Lu, X. F.; He, Q. J., Acid-Responsive H-2-Releasing 2D MgB2 Nanosheet for Therapeutic Synergy and Side Effect Attenuation of Gastric Cancer Chemotherapy. Adv Healthc Mater 2019, 8 (13). 23.Kou, Z.; Zhao, P. H.; Wang, Z. H.; Jin, Z. K.; Chen, L. H.; Su, B. L.; He, Q. J., Acid-responsive H-2-releasing Fe nanoparticles for safe and effective cancer therapy. J Mater Chem B 2019, 7 (17), 2759-2765. 24.Zhang, L.; Zhao, P. H.; Yue, C. P.; Jin, Z. K.; Liu, Q.; Du, X. B.; He, Q. J., Sustained release of bioactive hydrogen by Pd hydride nanoparticles overcomes Alzheimer's disease. Biomaterials 2019, 197, 393-404. 25.Yang, T.; Jin, Z. K.; Wang, Z. H.; Zhao, P. H.; Zhao, B.; Fan, M. J.; Chen, L. H.; Wang, T. F.; Su, B. L.; He, Q. J., Intratumoral high-payload delivery and acid-responsive release of H-2 for efficient cancer therapy using the ammonia borane-loaded mesoporous silica nanomedicine. Appl Mater Today 2018, 11, 136-143. 26.Zhao, P. H.; Jin, Z. K.; Chen, Q.; Yang, T.; Chen, D. Y.; Meng, J.; Lu, X. F.; Gu, Z.; He, Q. J., Local generation of hydrogen for enhanced photothermal therapy. Nat Commun 2018, 9. 27.Glenk, G.; Reichelstein, S., Economics of converting renewable power to hydrogen (vol 4, pg 216, 2019). Nat Energy 2019, 4 (4), 347-347. 28.Demirci, U. B.; Miele, P., Chemical hydrogen storage: 'material' gravimetric capacity versus 'system' gravimetric capacity. Energ Environ Sci 2011, 4 (9), 3334-3341. 29.Yadav, M.; Singh, A. K.; Tsumori, N.; Xu, Q., Palladium silica nanosphere-catalyzed decomposition of formic acid for chemical hydrogen storage. J Mater Chem 2012, 22 (36), 19146-19150. 30.Chen, P.; Xiong, Z. T.; Luo, J. Z.; Lin, J. Y.; Tan, K. L., Interaction of hydrogen with metal nitrides and imides. Nature 2002, 420 (6913), 302-304. 31.Rosi, N. L.; Eckert, J.; Eddaoudi, M.; Vodak, D. T.; Kim, J.; O'Keeffe, M.; Yaghi, O. M., Hydrogen storage in microporous metal-organic frameworks. Science 2003, 300 (5622), 1127-1129. 32.Zou, Y. Q.; von Wolff, N.; Anaby, A.; Xie, Y. J.; Milstein, D., Ethylene glycol as an efficient and reversible liquid-organic hydrogen carrier. Nat Catal 2019, 2 (5), 415-422. 33.Hoang, T. K. A.; Morris, L.; Sun, J.; Trudeau, M. L.; Antonelli, D. M., Titanium hydrazide gels for Kubas-type hydrogen storage. J Mater Chem A 2013, 1 (6), 1947-1951. 34.Hamaed, A.; Van Mai, H.; Hoang, T. K. A.; Trudeau, M.; Antonelli, D., Functionalized Porous Silicas with Unsaturated Early Transition Metal Moieties as Hydrogen Storage Materials: Comparison of Metal and Oxidation State. J Phys Chem C 2010, 114 (18), 8651-8660. 35.Ren, J. W.; Musyoka, N. M.; Langmi, H. W.; Mathe, M.; Liao, S. J., Current research trends and perspectives on materials-based hydrogen storage solutions: A critical review. Int J Hydrogen Energ 2017, 42 (1), 289-311. 36.Lopez-Corral, I.; Irigoyen, B.; Juan, A., Bonding in PdH2 and Pd2H2 systems adsorbed on carbon nanotubes: Implications for hydrogen storage. Int J Hydrogen Energ 2014, 39 (16), 8780-8790. 37.Hamaed, A.; Hoang, T. K. A.; Trudeau, M.; Antonelli, D. M., Optimization of hydrogen storage capacity in silica-supported low valent Ti systems exploiting Kubas binding of hydrogen. J Organomet Chem 2009, 694 (17), 2793-2800. 38.Yu, X. B.; Tang, Z. W.; Sun, D. L.; Ouyang, L. Z.; Zhu, M., Recent advances and remaining challenges of nanostructured materials for hydrogen storage applications. Prog Mater Sci 2017, 88, 1-48. 39.Zhang, X.; Ren, Z. H.; Zhang, X. L.; Gao, M. X.; Pan, H. G.; Liu, Y. F., Triggering highly stable catalytic activity of metallic titanium for hydrogen storage in NaAlH4 by preparing ultrafine nanoparticles. J Mater Chem A 2019, 7 (9), 4651-4659. 40.Li, L. L.; Yang, M.; Dong, Y.; Mei, P.; Cheng, H. S., Hydrogen storage and release from a new promising Liquid Organic Hydrogen Storage Carrier (LOHC): 2-methylindole. Int J Hydrogen Energ 2016, 41 (36), 16129-16134. 41.Carraro, P. M.; Sapag, K.; Oliva, M. I.; Eimer, G. A., Comparative study of hydrogen storage on metal doped mesoporous materials. Chem Phys Lett 2018, 701, 93-97. 42.Kamegawa, T.; Nakaue, T., Complete hydrogen release from aqueous ammonia-borane over a platinum-loaded titanium dioxide photocatalyst. Chem Commun 2015, 51 (94), 16802-16805. 43.Hamaed, A.; Trudeau, M.; Antonelli, D. M., H-2 storage materials (22KJ/mol) using organometallic Ti fragments as sigma-H-2 binding sites. J Am Chem Soc 2008, 130 (22), 6992-6999. 44.Navlani-Garcia, M.; Mori, K.; Kuwahara, Y.; Yamashita, H., Recent strategies targeting efficient hydrogen production from chemical hydrogen storage materials over carbon-supported catalysts. Npg Asia Mater 2018, 10, 277-292. 45.Zhang, L. J.; Xia, G. L.; Ge, Y.; Wang, C. Y.; Guo, Z. P.; Li, X. G.; Yu, X. B., Ammonia borane confined by nitrogen-containing carbon nanotubes: enhanced dehydrogenation properties originating from synergetic catalysis and nanoconfinement. J Mater Chem A 2015, 3 (41), 20494-20499. 46.Yan, J. M.; Zhang, X. B.; Han, S.; Shioyama, H.; Xu, Q., Magnetically recyclable Fe-Ni alloy catalyzed dehydrogenation of ammonia borane in aqueous solution under ambient atmosphere. J Power Sources 2009, 194 (1), 478-481. 47.Bluhm, M. E.; Bradley, M. G.; Butterick, R.; Kusari, U.; Sneddon, L. G., Amineborane-based chemical hydrogen storage: Enhanced ammonia borane dehydrogenation in ionic liquids. J Am Chem Soc 2006, 128 (24), 7748-7749. 48.Denney, M. C.; Pons, V.; Hebden, T. J.; Heinekey, D. M.; Goldberg, K. I., Efficient catalysis of ammonia borane dehydrogenation. J Am Chem Soc 2006, 128 (37), 12048-12049. 49.Keaton, R. J.; Blacquiere, J. M.; Baker, R. T., Base metal catalyzed dehydrogenation of ammonia-borane for chemical hydrogen storage. J Am Chem Soc 2007, 129 (7), 1844-+. 50.Xiong, Z. T.; Yong, C. K.; Wu, G. T.; Chen, P.; Shaw, W.; Karkamkar, A.; Autrey, T.; Jones, M. O.; Johnson, S. R.; Edwards, P. P.; David, W. I. F., High-capacity hydrogen storage in lithium and sodium amidoboranes. Nat Mater 2008, 7 (2), 138-141. 51.Peng, B.; Chen, J., Ammonia borane as an efficient and lightweight hydrogen storage medium. Energ Environ Sci 2008, 1 (4), 479-483. 52.Akbayrak, S.; Ozkar, S., Ammonia borane as hydrogen storage materials. Int J Hydrogen Energ 2018, 43 (40), 18592-18606. 53.Ge, Y. Z.; Shah, Z. H.; Lin, X. J.; Lu, R. W.; Liao, Z. M.; Zhang, S. F., Highly Efficient Pt Decorated CoCu Bimetallic Nanoparticles Protected in Silica for Hydrogen Production from Ammonia-Borane. Acs Sustain Chem Eng 2017, 5 (2), 1675-1684. 54.Verma, P.; Yuan, K.; Kuwahara, Y.; Mori, K.; Yamashita, H., Enhancement of plasmonic activity by Pt/Ag bimetallic nanocatalyst supported on mesoporous silica in the hydrogen production from hydrogen storage material. Appl Catal B-Environ 2018, 223, 10-15. 55.Yao, Q. L.; Lu, Z. H.; Zhang, Z. J.; Chen, X. S.; Lan, Y. Q., One-pot synthesis of core-shell Cu@SiO2 nanospheres and their catalysis for hydrolytic dehydrogenation of ammonia borane and hydrazine borane. Sci Rep-Uk 2014, 4. 56.Wang, Q.; Fu, F. Y.; Yang, S.; Moro, M. M.; Ramirez, M. D.; Moya, S.; Salmon, L.; Ruiz, J.; Astruc, D., Dramatic Synergy in CoPt Nanocatalysts Stabilized by 'Click' Dendrimers for Evolution of Hydrogen from Hydrolysis of Ammonia Borane. Acs Catal 2019, 9 (2), 1110-1119. 57.Yang, Y. W.; Lu, Z. H.; Hu, Y. J.; Zhang, Z. J.; Shi, W. M.; Chen, X. S.; Wang, T. T., Facile in situ synthesis of copper nanoparticles supported on reduced graphene oxide for hydrolytic dehydrogenation of ammonia borane. Rsc Adv 2014, 4 (27), 13749-13752. 58.Rakap, M.; Ozkar, S., Hydrogen generation from the hydrolysis of ammonia-borane using intrazeolite cobalt(0) nanoclusters catalyst. Int J Hydrogen Energ 2010, 35 (8), 3341-3346. 59.Liu, P. L.; Gu, X. J.; Kang, K.; Zhang, H.; Cheng, J.; Su, H. Q., Highly Efficient Catalytic Hydrogen Evolution from Ammonia Borane Using the Synergistic Effect of Crystallinity and Size of Noble-Metal-Free Nanoparticles Supported by Porous Metal Organic Frameworks. Acs Appl Mater Inter 2017, 9 (12), 10759-10767. 60.Torney, F.; Trewyn, B. G.; Lin, V. S. Y.; Wang, K., Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nat Nanotechnol 2007, 2 (5), 295-300. 61.Giri, S.; Trewyn, B. G.; Stellmaker, M. P.; Lin, V. S. Y., Stimuli-responsive controlled-release delivery system based on mesoporous silica nanorods capped with magnetic nanoparticles. Angew Chem Int Edit 2005, 44 (32), 5038-5044. 62.Gruenhagen, J. A.; Lai, C. Y.; Radu, D. R.; Lin, V. S. Y.; Yeung, E. S., Real-time imaging of tunable adenosine 5-triphosphate release from an MCM-41-type mesoporous silica nanosphere-based delivery system. Appl Spectrosc 2005, 59 (4), 424-431. 63.Watermann, A.; Brieger, J., Mesoporous Silica Nanoparticles as Drug Delivery Vehicles in Cancer. Nanomaterials-Basel 2017, 7 (7). 64.Tang, F. Q.; Li, L. L.; Chen, D., Mesoporous Silica Nanoparticles: Synthesis, Biocompatibility and Drug Delivery. Adv Mater 2012, 24 (12), 1504-1534. 65.Wan, Y.; Zhao, D. Y., On the controllable soft-templating approach to mesoporous silicates. Chem Rev 2007, 107 (7), 2821-2860. 66.Slowing, I. I.; Trewyn, B. G.; Lin, V. S. Y., Mesoporous silica nanoparticles for intracellular delivery of membrane-impermeable proteins. J Am Chem Soc 2007, 129 (28), 8845-8849. 67.Xia, T. A.; Kovochich, M.; Liong, M.; Meng, H.; Kabehie, S.; George, S.; Zink, J. I.; Nel, A. E., Polyethyleneimine Coating Enhances the Cellular Uptake of Mesoporous Silica Nanoparticles and Allows Safe Delivery of siRNA and DNA Constructs. Acs Nano 2009, 3 (10), 3273-3286. 68.Kim, M. H.; Na, H. K.; Kim, Y. K.; Ryoo, S. R.; Cho, H. S.; Lee, K. E.; Jeon, H.; Ryoo, R.; Min, D. H., Facile Synthesis of Monodispersed Mesoporous Silica Nanoparticles with Ultralarge Pores and Their Application in Gene Delivery. Acs Nano 2011, 5 (5), 3568-3576. 69.Hong, Y.; Chen, S.; Zhang, J. M., Hydrogen as a Selective Antioxidant: a Review of Clinical and Experimental Studies. J Int Med Res 2010, 38 (6), 1893-1903. 70.Ara, J.; Fadriquela, A.; Ahmed, M. F.; Bajgai, J.; Sajo, M. E. J.; Lee, S. P.; Kim, T. S.; Jung, J. Y.; Kim, C. S.; Kim, S. K.; Shim, K. Y.; Lee, K. J., Hydrogen Water Drinking Exerts Antifatigue Effects in Chronic Forced Swimming Mice via Antioxidative and Anti-Inflammatory Activities. Biomed Res Int 2018, 2018. 71.Chen, M. H.; Zhang, J.; Chen, Y.; Qiu, Y.; Luo, Z.; Zhao, S. X.; Du, L.; Tian, D. B., Hydrogen protects lung from hypoxia/re-oxygenation injury by reducing hydroxyl radical production and inhibiting inflammatory responses. Sci Rep-Uk 2018, 8. 72.Runtuwene, J.; Amitani, H.; Amitani, M.; Asakawa, A.; Cheng, K. C.; Inui, A., Hydrogen-water enhances 5-fluorouracil-induced inhibition of colon cancer. Peerj 2015, 3. 73.Wang, D. C.; Wang, L. F.; Zhang, Y.; Zhao, Y. X.; Chen, G., Hydrogen gas inhibits lung cancer progression through targeting SMC3. Biomed Pharmacother 2018, 104, 788-797. 74.Zhan, W. W.; Zhu, Q. L.; Xu, Q., Dehydrogenation of Ammonia Borane by Metal Nanoparticle Catalysts. Acs Catal 2016, 6 (10), 6892-6905. 75.Thakkar, S.; Sharma, D.; Kalia, K.; Tekade, R. K., Tumor microenvironment targeted nanotherapeutics for cancer therapy and diagnosis: A review. Acta Biomater 2020, 101, 43-68. 76.Kato, Y.; Ozawa, S.; Miyamoto, C.; Maehata, Y.; Suzuki, A.; Maeda, T.; Baba, Y., Acidic extracellular microenvironment and cancer. Cancer Cell Int 2013, 13. 77.Sharma, S. V.; Bell, D. W.; Settleman, J.; Haber, D. A., Epidermal growth factor receptor mutations in lung cancer. Nat Rev Cancer 2007, 7 (3), 169-181. 78.Jain, R. K.; Duda, D. G.; Clark, J. W.; Loeffler, J. S., Lessons from phase III clinical trials on anti-VEGF therapy for cancer. Nat Clin Pract Oncol 2006, 3 (1), 24-40. 79.Yuan, M.; Huang, L. L.; Chen, J. H.; Wu, J.; Xu, Q., The emerging treatment landscape of targeted therapy in non-small-cell lung cancer. Signal Transduct Tar 2019, 4. 80.Zugazagoitia, J.; Guedes, C.; Ponce, S.; Ferrer, I.; Molina-Pinelo, S.; Paz-Ares, L., Current Challenges in Cancer Treatment. Clin Ther 2016, 38 (7), 1551-1566. 81.Leonetti, A.; Sharma, S.; Minari, R.; Perego, P.; Giovannetti, E.; Tiseo, M., Resistance mechanisms to osimertinib in EGFR-mutated non-small cell lung cancer. Brit J Cancer 2019, 121 (9), 725-737. 82.Rotow, J.; Bivona, T. G., Understanding and targeting resistance mechanisms in NSCLC. Nat Rev Cancer 2017, 17 (11), 637-658. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17054 | - |
| dc.description.abstract | 早在 1975 年氫氣被發現具有治療小鼠皮膚麟狀癌的功能後，便揭開了科學家們對氫氣在生物醫學的一系列研究。氫氣的主要功能包括：(1) 中和生物體內活性氧化物(ROS)達到抗氧化的效果，(2) 調控發炎因子以抑制發炎反應，(3) 藉由調節生長因子或細胞凋亡因子以抑制腫瘤的生長。氫氣的給藥方式可分為口服(喝氫水)，吸入(吸氫氣)及注射(氫氣食鹽水)三種，但由於氫氣的分子極小、容易溢散且滯留性差，因此限制了氫氣治療的效果。此外，如何運送氫氣標靶至腫瘤細胞也是亟欲改善的重點。因此，在本研究的目標為開發可以有效治療癌症的新型產氫奈米載體。此奈米載體主要由三項元件組合而成：(1) 以硼烷氨(ammonia borane, AB)為儲氫材料；(2) 中孔奈米矽球(MSN)為藥物載體；(3) 以金奈米粒子催化 AB 產氫的效率。我們的初步實驗成果證實此一載體可以在腫瘤微環境中酸性環境下(pH 6.5〜6.9)增強金奈米粒子催化 AB 的效率，並藉由 MSN 賦予其標靶性，而後因為氫氣可從載體被緩效釋放，增加滯留性，三效合一使該產氫奈米載體可以累積在病灶提供持續性氫氣治療，達到顯著抑制癌細胞生長的效果。 | zh_TW |
| dc.description.abstract | Hydrogen was first reported in 1975 to have therapeutic effects in a skin squamous carcinoma mouse model. The main function of hydrogen as a therapeutic agent was to selectively quench detrimental reactive oxygen species (ROS) resulted in antioxidation. Furthermore, molecular hydrogen was able to regulate inflammatory factors to inhibit the inflammation, and to regulate growth/apoptotic factors to impede tumor growth. The common methods of hydrogen administration include oral route (drinking H2-dissolved water), inhalation (inhaling hydrogen gas), and intravenous injection (injecting H2-dissolved saline); however, the high diffusibility and low solubility of hydrogen often limited the efficacy of hydrogen therapy. Therefore, we believe that a nanomedicine strategy tailors to need by specific disease conditions would be a perfect solution for it. Our nanocarrier is composed of three components: the first is ammonia borane (AB), serving as a hydrogen producer, and the second is mesoporous silica nanoparticle, functioning as a carrier, the third is the gold nanoparticles, participating in speeding up the hydrogen production. Also this carrier is acid-responsive, the tumor microenviroment (TME)-triggered drug release enabled the efficient generation of H2. Preliminary results confirmed that our nanomedicine strategy enhance outcome of hydrogen therapy. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T23:54:50Z (GMT). No. of bitstreams: 1 U0001-0708202014363900.pdf: 4473769 bytes, checksum: 7b58201c0acefdaf57e93337d6cbde67 (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 目錄 第一章 緒論 1 1.1 當前癌症治療方法 1 1.2 氫氣治療發展 3 1.3 氫氣治療可降低生物體內活性氧化物 ( ROS ) 及調控抗氧化相關酵素 5 1.4 氫氣治療可調控之訊號分子 7 1.5 儲氫及產氫奈米載體的發展 8 1.6 產氫化合物-硼烷氨 10 1.7 介孔二氧化矽奈米粒子 10 第二章 研究動機，方向與實驗設計 12 2.1 研究動機 12 2.2 實驗設計之方向與設計 13 第三章 實驗材料與方法 17 3.1 細胞株及細胞培養 17 3.1.1 胚胎成纖維細胞-NIH/3T3 ( BCRC Number:60008 ) 17 3.1.2 路易士肺腺癌細胞-LL/2 (BCRC Number:60050) 17 3.1.3 細胞培養 18 3.1.4 凍細胞與細胞解凍 18 3.2 台盼藍染色 (Trypan blue staining) 18 3.3 細胞存活率測試(MTT cell viability assay) 19 3.4 細胞mRNA萃取與反轉錄 (Cell’s mRNA extraction and Reverse Transcription) 20 3.4.1 mRNA萃取 20 3.4.2 mRNA定量 21 3.4.3 反轉錄 21 3.5 聚合酶鏈鎖反應 (Polymerase Chain Reaction, PCR) 21 3.6 西方點墨法 ( Western blotting assay ) 24 3.6.1 蛋白質萃取 24 3.6.2 蛋白質定量 24 3.6.3 蛋白質電泳凝膠 ( SDS-PAGE ) 製備 25 3.6.4 SDS-PAGE蛋白質膠體電泳條件 25 3.6.5 蛋白質樣本轉印 26 3.6.6 免疫墨點法 ( Immuno-blotting ) 26 3.7 Caspase-3 Assay Kit ( Colorimetric ) ( 購自伯森生物科技 ) 27 3.8 流式細胞儀分析 ( Flow cytometric analysis ) 27 3.9 氫氣濃度測定 28 3.10 細胞內氧化自由基測定 ( Intracellular reactive oxygen species；ROS ) 30 3.11 產氫奈米載體合成 ( AB-Au@MSN ) 31 3.11.1 介孔二氧化矽奈米粒子合成 ( mesoporous silica nanoparticles, MSN ) 31 3.11.2 APTMS-MCM48制備 32 3.11.3 Au-APTMS-MCM48製備 34 3.11.4 硼烷氨含浸 35 3.12 產氫奈米載體之產氫效率測試 36 3.13 即時無標記細胞分析 (RTCA) 38 3.14 實驗材料 39 3.14.1 藥品 39 3.14.2 試劑 41 3.14.3 抗體 41 3.14.4 實驗儀器 42 第四章 實驗結果與討論 44 4.1 建立氫氣治療細胞平台 44 4.2 氫氣及產氫奈米載體對細胞存活率的影響 45 4.3 以RTCA分析產氫奈米載體於pH 7.4及pH 6對癌細胞之抑制效果 50 4.4 氫氣對細胞生長因子的調控 52 4.5 以流式細胞儀分析探討氫氣對細胞死亡路徑之影響 ( Annexin V / PI ) 54 4.6 氫氣對細胞凋亡的調控 57 4.7 氫氣對細胞焦亡的調控 60 第五章 結果討論及未來展望 64 第六章 參考文獻 65 | |
| dc.language.iso | zh-TW | |
| dc.title | 開發新型產氫奈米載體應用於癌症治療之研究 | zh_TW |
| dc.title | Development of novel hydrogen gas generating nanoparticle for cancer therapy | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳立真(Li-chen Wu),徐士蘭(Shih-Lan Hsu),呂瑞梅(Jui-Mei Lu),楊家銘(Chia-Min Yan) | |
| dc.subject.keyword | 氫氣治療,產氫奈米載體,抗氧化,抑制發炎反應,調節生長因子或細胞凋亡因子,抑制癌細胞生長, | zh_TW |
| dc.subject.keyword | Hydrogen therapy,nanomedicine,antioxidation,inhibit the inflammation,regulate growth/apoptotic factors,impede tumor growth, | en |
| dc.relation.page | 74 | |
| dc.identifier.doi | 10.6342/NTU202002634 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2020-08-11 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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