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標題: | 電噴霧製程技術製備功能性聚對二甲苯微奈米粒子之開發應用 Micro- and Nano- Particles Based on Functionalized Poly-para-xylylenes via Electrospraying |
作者: | Hsin-Ying Ho 何信穎 |
指導教授: | 陳賢燁(Hsien-Yeh Chen) |
關鍵字: | 電噴霧製程技術,功能性聚對二甲苯,微奈米粒子,生物耦合技術,表面改質, electrospraying technique,functionalized poly-para-xylylene,micro- to nano- particle,bioconjugation,surface modification, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 微奈米粒子技術的成熟賦予了許多材料截然不同的性質,而在工業上的應用也漸趨豐富,此外,近年來生物技術以及奈米醫藥的發展獲得許多重要的突破以及成功的應用,而在這其中微奈米技術的參與更是不可或缺,然而,在廣大的高分子領域當中,功能性聚對二甲苯高分子微奈米粒子 (functionalized poly-para-xylylene particle) 首次利用電噴霧製程技術 (electrospraying technique) 由苯甲醯基聚對二甲苯 (poly[(4-benzoyl-p-xylylene)-co-(p-xylylene)]) 成功地製備出來。一系列的分析包括動態光散射 (dynamic light scattering) 以及介面電位 (zeta potential) 將被用來驗證粒子之粒徑及表面電荷,掃描式電子顯微鏡 (scanning electron microscope) 以及穿透式電子顯微鏡 (transmittance electron microscope) 將被用來分析粒子表面型態以及內部構造,傅立葉轉換紅外線光譜 (fourier transform infrared spectroscope) 將被用來分析粒子化學組成,共軛焦螢光顯微鏡 (confocal fluorescence microscope) 將被用來擷取螢光影像,石英晶體微天平 (quartz crystal microbalance) 將被用來測量粒子之重量。在成功製備出苯甲醯基聚對二甲苯微奈米粒子後,polyethylene glycol-biotin 率先修飾於粒子表面,接著以生物耦合技術專一性地將 Alexa Fluor 488 streptavidin 透過 biotin-streptavidin作用而結合,藉此證明表面官能基活性;而將粒子改質使具有生物功能性前亦進行細胞毒性測試,並以 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 進行細胞存活率分析,最後,此一功能性聚對二甲苯微奈米粒子將被設計並且改質以展示不同之生物功能,其一為將奈米粒子表面修飾有抗癌藥物 paclitaxel 進行癌細胞毒殺測試,其二為將奈米粒子表面修飾有抗菌藥物 chlorhexidine 進行最小抑菌濃度測試。未來除了苯甲醯基聚對二甲苯外,各式各樣具有不同功能性的聚對二甲苯亦能夠以微奈米粒子的形式進行多樣的應用,更重要的是,透過製程變化能夠使微奈米粒子由單功能性進化成多功能性以滿足漸趨複雜之改質設計,然而,不同功能性的官能基於粒子表面的分布亦能夠以混合或者區域性的方式呈現,藉此提升微奈米粒子改質設計的層次。 Nanotechnology has led numerous materials to have their brand new properties and those materials have been widely applied in industry. Besides, with the help of nanotechnology, the field of biotechnology and nanomedicine has gain many important breakthrough. However, in the field of macromolecules, functionalized poly-para-xylylene particle was first successfully manufactured by electrospraying technique with poly[(4-benzoyl-p-xylylene)-co-(p-xylylene)], benzoyl-PPX. Dynamic light scattering and zeta potential tests were performed to measure the diameter and surface charge of vary-sized micro- to nano- particle. Scanning electron microscope and transmittance electron microscope were used to make sure the surface morphology and the interior structure of benzoyl-PPX particles. Fourier transform infrared spectroscope was also performed to analyze the particle chemistry characteristics. Confocal fluorescence microscope was use as a tool to get fluorescence photo of particle. Quartz crystal microbalance was used to weight the particle powder. Polyethylene glycol-biotin was first attach to particle by UV light induced photochemistry reaction, then fluorescence Alexa Fluor 488 streptavidin was combined with PEG-biotin through bioconjugation reaction specifically. Before bio-test to be conduct, particles of varying size were used to undergo cytotoxicity test followed by MTT assay. Finally, this functionalized poly[(4-benzoyl-p-xylylene)-co-(p-xylylene)] particle was design to exhibit distinct biological functions by first immobilizing anti-cancer drug paclitaxel and serving as cancer cell killer. Secondly, anti-bacteria drug chlorhexidine was also immobilized onto particle surface to suppress the growth of bacteria. In the future, many kinds of functionalized poly-para-xylylene particle in addition to benzoyl-PPX particle will have different applications. More importantly, mono-functionalized particle can turn into multi-functionalized particle with the adjustment of electrospraying technique. However, the different functional groups can also be distribute in the way of mixed or biphasic on the surface of particle, which will greatly meet the requirement of more complex particle design. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16658 |
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顯示於系所單位: | 化學工程學系 |
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