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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王淑美(Shue-Mei Wang) | |
| dc.contributor.author | Yu-Shan Liu | en |
| dc.contributor.author | 劉瑜珊 | zh_TW |
| dc.date.accessioned | 2021-06-07T18:18:13Z | - |
| dc.date.copyright | 2012-03-19 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-02-06 | |
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Opin. plant Bio. 11: 541-571. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16508 | - |
| dc.description.abstract | 開花，是由植物體內局部性或系統性的開花訊息因子所共同調控。其中最廣為人知的開花素「FLOWERING LOCUS T (FT)」，會受到光週期的刺激而活化，並由葉片經韌皮部長距離的運送至莖頂而誘導開花。近來的微陣列結果與嫁接實驗發現，「AGAMOUS-LIKE 24 (AGL24)」與「FVE」兩個阿拉伯芥開花基因的RNA亦可由轉殖株砧木長距離移動至接穗中。因此，本篇研究主要目的為探討內源的AGL24 與 FVE RNA 在植物中長距離移動之能力，並針對其誘導開花的功能性作進一步的研究。為了解 AGL24 及 FVE 的非細胞自主 (non-cell autonomous) 功能性，我利用具組織專一性的啟動子在突變株中改變 AGL24 及 FVE 表現量。藉此互補實驗發現韌皮部中 AGL24 與 FVE 的表現量改變時，會影響到植物莖頂的開花時間。此外，利用阿拉伯芥嫁接方式及反轉錄聚合酶連鎖反應(RT-PCR) 的分析，證實內源的 AGL24 RNA 具有於韌皮部中長距離運送的能力。而利用阿拉伯芥轉殖技術及共軛焦顯微鏡觀察結果得知，FVE 的蛋白質可能具有從韌皮部的伴細胞運送至表皮細胞的能力。因此，我推測 AGL24 與 FVE 可能在植物的開花途徑中，扮演著系統性開花調節因子的角色。 | zh_TW |
| dc.description.abstract | Floral initiation is regulated by many local and systemic florigenic signals. In addition to FLOWERING LOCUS T (FT), which mediates systemic photoperiodic signal, evidences show that other systemic floral regulators may participate in floral induction. Previous microarray analysis reveals that the RNA of some floral regulators is presented in broccoli phloem sap. Further Arabidopsis grafting experiments demonstrate that at least two phloem sap RNA, AGAMOUS-LIKE 24 (AGL24) and FVE, moves long-distance in transformants. In this study, we aim to investigate the systemic florigenic avtivity of endogenous AGL24 and FVE RNA. To determine the non-cell autonomous function of AGL24 and FVE, we ectopically expressed the AGL24 and FVE in comanion cells and found that the expression of AGL24 and FVE in companion cells is sufficient to promote floral initiation. We also examined the RNA movement of endogenous AGL24 and FVE and found that endogenous AGL24 RNA is a non-cell autonomous RNA. In addition, our preliminary results demonstrated that the FVE protein may be a non-cell autonomous protein. In conclusion, our data showed that AGL24 and FVE may participate in systemic floral regulation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T18:18:13Z (GMT). No. of bitstreams: 1 ntu-101-R98b42032-1.pdf: 3593532 bytes, checksum: 0f5e1f089ad0ca1980e903eee684773a (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 I 致謝 II 中文摘要 III 英文摘要 IV 第一章 前言 1 植物韌皮部運輸系統的結構與特性 1 開花素 FT 的發現與研究 2 其他系統性開花調節因子參與開花的調控 3 研究目的 5 第二章 材料與方法 7 植物材料 7 質粒 (plasmid) DNA 之構築 7 植物 RNA 之萃取 7 反轉錄反應 (Reverse transcription, RT) 8 聚合酶連鎖反應 (polymerase chain reaction, PCR) 8 由瓊脂膠體回收並純化 DNA 片段 9 DNA 黏合反應 (ligation) 9 E. coli質粒 (plasmid) DNA mini-prep 10 轉型作用 (transformation) 10 製備農桿菌勝任細胞 (competent cells) 11 阿拉伯芥之轉殖 11 植物基因組 (genomic) DNA 之製備 12 選殖 FVE 與 AGL24 之 artificial miRNA (amiRNA) 12 阿拉伯芥開花枝嫁接法 15 GUS 之染色分析法 15 共軛焦顯微鏡 (Confocal Microscope) 16 第三章 結果 17 第一部分 阿拉伯芥韌皮部中 AGL24 對開花之影響 17 提高韌皮部中 AGL24 表現量可促進植物開花 17 降低韌皮部中 AGL24 表現量對開花時間之影響 18 AGL24 RNA 之長距離運輸能力 19 第二部分 阿拉伯芥韌皮部中 FVE 對開花之影響 23 增加韌皮部中 FVE 表現量可促進植物開花 23 降低韌皮部中 FVE 表現量會延遲植物的開花 24 FVE 基因產物之長距離運輸能力 25 第四章 討論 29 AGL24 可能為系統性的開花調節因子 29 FVE 可能參與在植物的系統性開花調控 30 植物韌皮部中非細胞自主 RNA 長距離運輸機制 31 總論 32 圖表 34 參考文獻 52 附錄 59 | |
| dc.language.iso | zh-TW | |
| dc.title | 阿拉伯芥開花基因AGL24與FVE參與系統性開花調控之研究 | zh_TW |
| dc.title | Study on systemic floral regulation by flowering genes AGL24 and FVE in Arabidopsis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 余天心(Tien-Shin Yu) | |
| dc.contributor.oralexamcommittee | 鄭石通(Shih-Tong Jeng) | |
| dc.subject.keyword | AGL24,FVE,長距離運輸,嫁接,阿拉伯芥, | zh_TW |
| dc.subject.keyword | AGL24,FVE,RNA movemen,grafting,Arabidopsis, | en |
| dc.relation.page | 60 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2012-02-07 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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