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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 郭彥彬 | |
dc.contributor.author | Hsiang-Yi Chung | en |
dc.contributor.author | 鍾享艗 | zh_TW |
dc.date.accessioned | 2021-06-07T18:10:28Z | - |
dc.date.copyright | 2012-09-17 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-07-09 | |
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Ectodomain shedding of TGF-alpha and other transmembrane proteins is induced by receptor tyrosine kinase activation and MAP kinase signaling cascades. EMBO J. 15;18(24):6962-72. Fujii, D., Brissenden, JE., Derynck, R., Francke, U. 1986. Transforming growth factor beta gene maps to human chromosome 19 long arm and to mouse chromosome 7. Somat Cell Mol Genet. 12(3):281-8. Gao, R., Brigstock, DR. 2003. Connective tissue growth factor (CCN2) induces adhesion of rat activated hepatic stellate cells by binding of its C-terminal domain to integrin alpha(v)beta(3) and heparan sulfate proteoglycan. J Biol Chem. 79(10):8848-55 Goseki-Sone, M., Tamada, A., Hamatani, R., Mizoi, L., Iimura, T. & Ezawa, I., 2002. Phosphate depletion enhances bone morphogenetic protein-4 gene expression a cultured mouse marrow stromal cell line st2. Biochemical and Biophysical Research Communications, 299 (3), 395-9. Hattersley, G., Chambers, TH., 1991. Effects of transforming growth factor beta 1 on the regulation of osteoclastic development and function. J Bone Miner Res, 6: 165-172. Heldin, C.H., Miyazono, K. & Ten Dijke, P., 1997. TGF-beta signaling from cell membrane to nucleus through smad proteins. Nature, 390 (6659), 465-71. Holmes, A. Abraham, DJ., Sa, S., Shiwen, X., Black, CM., Leask, A. 2001. CTGF and SMADs, maintenance of scleroderma phenotype is independent of SMAD signaling. J Biol Chem. 276(14):10594-601. Hung, P.S., Chen, F.C, Kuang, S.H., Kao, S.Y., Lin, S.C., Change, K.W. 2010. miR-146a induces differentiation of periodontal ligament cells. J Dent Res. 89(3): 252-257. Hurvitz, JR., Suwairi, WM., Van Hul W, El-Shanti, H., Superti-Furga, A., Roudier, J., et al. 1999. Mutations in the CCN gene family member VISP3 cause progressive pseudorheumatoid dysplasia. Nat Genet, 23:94-8 Jedsadayanmata, A., Chen, CC., Kireeva, ML., Lau, LF., Lam, SC. 1999. Activation-dependent adhesion of human platelets to Cyr61 and Fisp12/mouse connective tissue growth factor is mediated through integrin alpha(IIb)beta(3). J Biol Chem. 274(34):24321-7. Kadota, H., Nakanishi, T., Asaumi, K., Yamaai, T., Nakata, E., Mitani, S., Mitani, S., et al. 2004. Expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24/CCN2) during distraction osteogenesis. J Bone Miner Metab, 22: 293-302. Kawaki, H., Kubota, S., Suzuki, A., Yamada, T., Matsumura, T., Mandai, T., et al. 2008. Functional requirement of CCN2 for intramembranous bone formation in embryonic mice. Biochem Biophys Res Commun. 366: 450-6. Kawaki, H., Kubota, S., Suzuki, A., Suzuki, M., Kohsaka, K., Hoshi, K., Fujii, T., Lazar, N., Ohgawara, T., Maeda, T., Perbal, B., Yamamoto, T.T., Takigawa, M., 2011. Differential roles of CCN family proteins during osteoblast differentiation: Involvement of Smad and MAPK signaling pathways. Bone, 49 (5), 975-89. Kawase, T., Sato, S., Miake, K., Saito, S. 1988. Alkaline phosphatase of human periodontal ligament fibroblast-like cells. Adv Dent Res. 2(2):234-9. Kumar, S., Hand, AT., Connor, JR., Dodds, RA., Ryan, PJ., Trill, JJ., et al. 1999. Identification and cloning of a connective tissue growth factor-like cDNA from huma osteoblasts encoding a novel regulator of osteoblast functions. J Biol Chem, 274: 17123-31. Leask, A., Abraham, D.J.. 2003. The role of connective tissue growth factor, a multifunctional matricellular protein, in fibroblast biology. Biochem Cell Biol, 81 (6), 355-63. Lebrin, F., Deckers, M., Bertolino, P. & Ten Dijke, P., 2005. TGF-beta receptor function in the endothelium. Cardiovasc Res, 65 (3), 599-608. Lee, K.S., Hong, S.H. & Bae, S.C., 2002. Both the smad and p38 MAPK pathways play a crucial role in RunX2 expression following induction by transforming growth factor-beta and bone morphogenetic protein. Oncogene, 21 (47), 7156-63. Lind, M., 1998. Growth factor stimulation of bone healing. Effect on osteoblasts, osteomies, and implants fixation. Acta Orthop Scand Suppl, 283: 2-37. Massagué, J. 1990. Transforming growth factor-alpha. A model for membrane-anchored growth factors. J Biol Chem. 15;265(35):21393-6. Melcher, AH. 1970. Repair of wounds in the periodontium of the rat. Influence of periodontal ligament on osteogenesis. Arch Oral Biol. 15(12):1183-204. Nohutcu, RM., McCauley, LK., Koh, AJ., Somerman, MJ. 1997. Expression of extracellular matrix proteins in human periodontal ligament cells during mineralization in vitro. J Periodontol. 68(4):320-7. Ohshima, M., Kuwata, F., Otsuka, K., Saito, R., Sato, K., Shioji, S., Suzuki, K. 1988. Alkaline phosphatase activities of cultured human periodontal ligament cells. J Nihon Univ Sch Dent. 30(3):208-17. Samarakoon, R., Higgins, SP., Higgins, CE., Higgins, PJ. TGF-β1-induced plasminogen activator inhibitor-1 expression in vascular smooth muscle cells requires pp60c-src/EGFRY845 and Rho/ROCK signaling. J Mol Cell Cardiol. 44(3):527-38. Stavropoulo, A., Wikesjo, UME. 2012. Growth and differentiation factors for periodontal regeneration: a review on factors with clinical testing. J Periodontal Res. 2012 Apr 5. doi: 10.1111/j.1600-0765.2012.01478.x. [Epub ahead of print] Seo, BM., Miura, M., Gronthos, S., Bartold, PM., Batouli, S., Brahim, J., Young, M., Robey, PG., Wang, CY., Shi, S. 2004. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004 Jul 10-16;364(9429):149-55. Sloan, A.J., Rutherford, R.B. & Smith, A.J., 2000b. Stimulation of the rat dentine-pulp complex by bone morphogenetic protein-7 in vitro. Arch Oral Biol, 45 (2), 173-7. Sloan, AJ., Couble, ML., Bleicher, F., Magloire, H., Smith, AJ., Farges, JC. 2001. 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J Endod. 2008 Apr;34(4):427-32. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16339 | - |
dc.description.abstract | 牙周病的產生會破壞牙周組織的結構,減少牙齒周圍的支撐結構,最後導致牙齒的脫落,牙周病的治療從控制發炎及感染,近來擴展至再生治療的應用,以期恢復牙齒周遭的健康牙周組織。組織工程的進步,發展出許多生長因子的應用。TGF-β及CTGF,在人體或動物體中具有誘導骨再生的能力,。然而在不同組織具有相當的差異性。
研究目的:本研究探討在牙周韌帶細胞中,TGF-β及CTGF對牙周細胞骨再生能力的影響,了解這些生長因子對牙周組織的影響。並且探討TGF-β誘導CTGF訊息傳遞路徑。 材料與方法:本實驗利用牙周韌帶細胞,以TGF-β及CTGF去誘導細胞的骨分化,並檢測其基因表現及鹼性磷酸酶的活性。同時以TGF-β刺激牙周細胞,以西方墨點法檢測 CTGF的訊息傳遞路徑。 結果: TGF-β及CTGF會誘導牙周細胞骨分化基因 BMP2的表現。另外,TGF-β在牙周韌帶細胞中,會經由 JNK、Smad 3、Rac-1、及Src-family kinase 去誘導 CTGF的表現。 結論:TGF-β及CTGF會誘導牙周細胞骨分化基因 BMP2的表現,TGF-β同時會刺激CTGF的表現。因此,TGF-β可能會經由CTGF表現的路徑,去促進細胞的分化,誘導牙周組織的骨分化表現。 關鍵字:牙周再生,轉型生長因子β,結締組織生長因子 | zh_TW |
dc.description.abstract | Periodontal disease would led to destruction of periodontal tissue, reduce periodontal supporting structure, and finally led to tooth loss. The development of periodontal therapy starts from infection control to tissue regeneration in order to regenerate the healthy tissue surrounding tooth structure. Discovery from tissue engineering found many growth factors leading to tissue regeneration. Recently, transforming growthβ(TGF-β)and connective tissue growth factor (CTGF) can induce bone formation and osteoblast differentiation. However, previous studies show large variety among different cells and tissues
Objectivs: This study is design to find the influence of TGF-β and CTGF on the osteogenic differentiation of periodontal ligament cell,to understand the influence of these growth factors on periodontal tissue. Furthermore, we discover the signal transduction between TGF-β and CTGF to find the mechanism between two growth factors. Therefore, we want to realizing these mechanism and to apply these growth factors to clinical periodontal regeneration Methods: Immortalized periodontal ligament cells (iPDL) received from Dr. Change of National Yang-Ming University. TGF-β and CTGF were applied to induce the osteogenic differentiation of iPDL. Gene expression was analyzed by Real-time PCR and the protein of differentiation was examined by alkaline phosphatase stain. Further, TGF-β was added to induced CTGF expression in iPDL. CTGF, phosphorylated Smad3, and phosphorlated JNK protein expression were analyzed by western blotting. Results: TGF-β and CTGF can induce the osteogenic differentiation gene expression in iPDL. TGF-βalso can induce the alkaline phosphatase activity. Furthermore, in PDL, TGF-β can induce the expression of CTGF through JNK, Smad 3, Rac-1, and Src-family kinase. Conclusion: TGF-β and CTGF can induce osteogenic differentiation in periodntal tissue. TGF-β also can induce CTGF expression in iPDL. Therefore, TGF-β might induce PDL osteogenic differentiation through CTGF expression. Key words: periodontal regeneration, TGF-β, CTGF | en |
dc.description.provenance | Made available in DSpace on 2021-06-07T18:10:28Z (GMT). No. of bitstreams: 1 ntu-101-R98422021-1.pdf: 2842582 bytes, checksum: 61f4305e19e77dea54808a003a6bcf1b (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 目錄
誌謝 I 中文摘要 II Abstract III 目錄 V 圖目錄 VIII 第一章 緒論 1 第一節 牙周組織及其細胞 1 1-1 牙周組織 (Periodontium) 1 1-2 牙周韌帶細胞 (Periodontal ligament cell) 1 第二節 骨生理 (Bone physiology) 2 2-1 骨生成及分化過程 2 2-2 鹼性磷酸酶 (Alkaline phosphatase)與骨分化 3 2-3 RunX2與骨分化 4 2-4 成骨蛋白2 (BMP-2)與骨分化 4 第三節 轉型生長因子β (Transforming growth factor-β,TGF-β) 5 3-1 轉型生長因子β介紹 5 3-2 轉型生長因子β的訊息傳遞 6 3-3 轉型生長因子β與骨分化 7 第四節 結締組織生長因子(connective tissue growth factor,CTGF) 8 4-1 結締組織生長因子介紹 8 第二章 研究目的 10 第三章 實驗材料與方法 11 一、細胞株與細胞培養 11 二、藥物處理 12 2-1 轉型生長因子β與結締組織生長因子 12 2-2 Inhibitor使用資料 12 三、誘導牙周韌帶細胞分化 12 四、反轉錄聚合酶連鎖反應 (Reverse Transcription polymerase Chain Reaction;RT-PCR) 13 4-1 RNA萃取 13 4-2 RNA定量 14 4-3 反轉錄 (Reverse transcription;RT) 14 4-4 聚合酵素連鎖反應 (Polymerase Chain Reaction;PCR) 14 4-5 瓊脂凝膠電泳分析 (Agarose gel electrophoresis) 15 五、定量聚合酶連鎖反應 (Real-time Polymerase Chain Reaction;Real-time PCR;qPCR) 15 六、西方墨點法 (Western blot) 16 6-1 蛋白質萃取 16 6-2 蛋白質濃度測定 (Total protein analysis) 16 6-3 膠體配置與電泳分析 17 6-4 蛋白質轉漬 (Protein transfer) 17 6-5 抗體使用以及顯影 18 七、鹼性磷酸酶染色 (Alkaline phosphatase staining) 18 7-1 藥品配置 19 7-2 染色步驟 19 第四章 結果 20 一、不朽化牙周韌帶細胞分化能力 20 二、TGF-β誘導牙周韌帶細胞分化 20 1. TGF-β誘導牙周韌帶細胞分化的基因表現 20 2. TGF-β誘導化牙周韌帶細胞分化的鹼性磷酸酶表現 21 三、CTGF 誘導牙周韌帶細胞分化 22 1. CTGF誘導牙周韌帶細胞分化的基因表現 22 四、TGF-β刺激牙周韌帶細胞 CTGF蛋白的表現 22 1. TGF-β在不同濃度下誘導牙周韌帶細胞之 CTGF的表現 22 2. TGF-β在不同時間下誘導牙周韌帶細胞之 CTGF的表現 23 3.不同抑制劑處理隊 TGF-β誘導牙周韌帶細胞 CTGF表現的影響 23 4. 不同抑制劑處理對 TGF-β誘導不朽化牙周韌帶細胞 CTGF表現的影響 24 5. TGF-β於不同時間下誘導牙周韌帶細胞之 JNK磷酸化的表現 24 6. TGF-β於不同時間下誘導化牙周韌帶細胞之 Smad 3 磷酸化的表現 25 7. Rac-1 inhibitor 不影響 TGF-β於刺激牙周韌帶細胞之 JNK 磷酸化的表現 25 8.Src-family kinase inhibitor 不影響 TGF-β於刺激牙周韌帶細胞之 JNK 磷酸化的表現 26 9.Rac-1 inhibitor 不影響 TGF-β於刺激牙周韌帶細胞之 Smad3 磷酸化的表現 26 10.Src-family kinase inhibitor 不影響 TGF-β於刺激牙周韌帶細胞之 Smad3 磷酸化的表現 26 11. Rac-1 inhibitor 不影響 TGF-β於刺激牙周韌帶細胞之 Smad2 磷酸化的表現 27 12.Src-family kinase inhibitor 不影響 TGF-β於刺激牙周韌帶細胞之 Smad2 磷酸化的表現 27 第五章 討論 28 第六章 結論 31 參考文獻 48 | |
dc.language.iso | zh-TW | |
dc.title | 轉型生長因子β誘導牙周韌帶細胞骨分化表現之影響 | zh_TW |
dc.title | The influence of TGF-β on osteogenic differentiation in periodontal ligament cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 呂炫?,周涵怡 | |
dc.subject.keyword | 牙周再生,轉型生長因子β,結締組織生長因子, | zh_TW |
dc.subject.keyword | periodontal regeneration,TGF-β,CTGF, | en |
dc.relation.page | 56 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2012-07-09 | |
dc.contributor.author-college | 牙醫專業學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
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