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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 郭彥彬 | |
| dc.contributor.author | Cheng-Pang Lan | en |
| dc.contributor.author | 藍正邦 | zh_TW |
| dc.date.accessioned | 2021-06-07T18:07:43Z | - |
| dc.date.copyright | 2012-09-17 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-07-20 | |
| dc.identifier.citation | Adams, J.M., and Cory, S. (1998). The Bcl-2 protein family: arbiters of cell survival. Science (New York, NY) 281, 1322-1326.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16274 | - |
| dc.description.abstract | 牙科治療裡，利多卡因最常被用在牙科局部麻醉中。伴隨而來的併發症有顏面神經損傷、牙關緊閉、軟組織傷害等。文獻中探討利多卡因對細胞毒性與死亡的機轉並不十分清楚。
實驗目的：本研究探討以人類胚胎上顎間質細胞作為模式，了解利多卡因是否能造成口腔細胞凋亡或細胞自噬反應。同時更進一步研究其細胞凋亡背後的機轉與路徑。 材料與方法：利用細胞活性分析、西方點墨法、流式細胞分析TUNEL染色，不同濃度與時間，測試利多卡因對人類胚胎上顎間質細胞的影響 結果： 利多卡因會誘導人類胚胎上顎間質細胞進行細胞凋亡與自體吞噬反應，凋亡細胞與時間濃度增加成正比。不過，加入Caspase-8 抑制劑, Z-IETD-FMK, Caspase-9抑制劑, Z-LEHD-FMK, Pan-caspase抑制劑, Q-VD-OPH，都無法阻擋細胞進行細胞凋亡。此外加入ROS抑制劑與MAPK抑制劑也同樣無法使細胞活性恢復。另外，根據西方點墨法的結果，利多卡因同時也能誘導人類胚胎上顎間質細胞進行細胞自體吞噬反應。 結論：利多卡因會誘導人類胚胎上顎間質細胞進行細胞凋亡與細胞自體吞噬反應，但需要更多的研究來了解利多卡因對細胞死亡的機轉。 | zh_TW |
| dc.description.abstract | Lidocaine is one of the most used local anesthetics in dentistry. Although this is rare, a number of potential complications are associated with the administration of local anesthetics including facial nerve paralysis, trismus, soft-tissue injury, pain on injection.
Objectives：We investigated the mechanism of Lidocaine induced apoptosis in human embryonic palatal mesenchymal cells(HEPM) and try to identify possible signaling pathways that mediate their effects. In addition, we want to investigate any activation of autophagy in human embryonic palatal mesenchymal cells treated with Lidocaine. Methods: Cell viability assay, flow cytometry, TdT-mediated dUTP nickend labeling assay and Western blot analysis were used to investigate the effects of Lidocaine on human embryonic palatal mesenchymal cells. Results：We found Lidocaine induced apoptosis in dose and time dependent manner. However, after pretreated Caspase-8 inhibitor, Z-IETD-FMK, Caspase-9 inhibitor, Z-LEHD-FMK, Pan-caspase inhibitor, Q-VD-OPH then incubated in Lidocaine with HEPM, neither inhibitors can block Lidocaine induced apoptosis. In addition, ROS inhibitors and MAPK family inhibitors also failed to reverse cell viability in HEPM treated with Lidocaine. Meanwhile, western blotting showed that LC3-II was increased from the conversion of LC3-I in time dependent manners. It means Lidocaine also elicited autophagy in HEPM. Conclusion：It is concluded that Lidocaine induced both apoptosis and autophagy in human embryonic palatal mesenchymal cells. Further research may need to elucidate the Lidocaine induced cell death. Key words: Lidocaine, HEPM, Apoptosis, Autophagy | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T18:07:43Z (GMT). No. of bitstreams: 1 ntu-101-R98422006-1.pdf: 1744050 bytes, checksum: 0640919bb0a4ef9306ba7a3abda35fbc (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 誌謝 I
中文摘要 III Abstract英文摘要 IV 目錄 V 圖目錄 VII 第一章 緒論 1 1-1 Lidocaine 簡介 Introduction of Lidocaine 1 1-1-1 Lidocaine 的藥理機轉 Pharmacology of Lidocaine 1 1-1-2 Lidocaine 的細胞毒性 Cytotoxicity of Lidocaine 2 1-2 牙科局部麻醉的併發症Complication of dental anesthetics 3 1-3 細胞凋亡 Apoptosis 4 1-4細胞凋亡之分子機制Molecular mechanisms of apoptosis 4 1-5 MAPK (Mitogen-activated protein kinase)pathway與細胞凋亡 6 1-6 細胞自體吞噬 Autophagy 7 1-7 細胞自體吞噬的分子機轉Molecular mechanism in autophagy 8 第二章 實驗目的 9 第三章 實驗材料與方法 10 3-1 細胞株與細胞培養(Cell culture) 10 3-2細胞存活率試驗 (MTT) assay 10 3-2-1 Inhibitor藥物的使用資料 11 3-3 西方墨點法 11 3-3-1 蛋白質萃取 11 3-3-2 膠體配置與電泳分析 12 3-3-3 蛋白轉漬 12 3-3-4 抗體的使用與顯影呈色 12 3-4 利用TUNEL (Temianl deoxynucleotidyl Transferase-mediated dUTP –biotin nick end labeling) 偵測細胞凋亡 13 第四章 結果 14 4-1 Lidocaine抑制人類胚胎上顎間質細胞生長 14 4-2 Lidocaine 誘導人類胚胎上顎間質細胞凋亡反應 14 4-3 Caspase抑制劑對Lidocaine誘導人類胚胎上顎間質細胞凋亡的影響 15 4-4 ROS(Reactive oxygen species)、MAPK(Mitogen-Activated ProteinKinase) pathway對人類胚胎上顎間質細胞凋亡的影響 15 4-5 Lidocaine 誘導人類胚胎上顎間質細胞自體吞噬反應 16 第五章討論及未來展望 17 第六章 結論 18 第七章 圖與表 19 參考文獻 34 | |
| dc.language.iso | zh-TW | |
| dc.title | 利多卡因誘導人類胚胎上顎間質細胞凋亡之研究 | zh_TW |
| dc.title | Lidocaine Induced Apoptosis in Human Embryonic Palatal Mesenchymal Cell | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 呂炫?,周涵怡 | |
| dc.subject.keyword | 利多卡因,人類胚胎上顎間質細胞,細胞凋亡,細胞自體吞噬反應, | zh_TW |
| dc.subject.keyword | Lidocaine,HEPM,Apoptosis,Autophagy, | en |
| dc.relation.page | 41 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2012-07-20 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
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