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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 曾四恭 | |
dc.contributor.author | Wen-Po Tsou | en |
dc.contributor.author | 鄒文博 | zh_TW |
dc.date.accessioned | 2021-06-07T18:02:33Z | - |
dc.date.copyright | 2012-08-28 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-03 | |
dc.identifier.citation | 1. Amann, R., J. Snaidr, M. Wagner, W. Ludwig, and K. H. Schleifer. 1996. In situ visualization of high genetic diversity in a natural microbial community. Journal of Bacteriology 178:3496-3500.
2. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Applied and Environmental Microbiology 56:1919-1925. 3. Amann, R. I., W. Ludwig, and K. H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiology Review 59:143-169. 4. Anderson, M. L. M. and B. D. Young. 1985. Quantitative filter hybridization, p. 73-113. In B. D. a. S. J. H. Hames (ed.), Nucleic acid hybridization. IRL Press. UK. 5. Anthonisen, A. C., R. C. Loehr, T. B. S. Prakasam, and E. G. Srinath. 1976. Inhibition of nitrification by ammonia and nitrous acid. Journal Water Pollution Control Federation 48:835-852. 6. Arrojo, B., A. Mosquera-Corral, J. L. Campos, and R. Mendez. 2006. Effects of mechanical stress on Anammox granules in a sequencing batch reactor (SBR). Journal of Biotechnology 123:453-463. 7. Asano, H., H. Myoga, M. Asano, and M. Toyao. 1992. A study of nitrification utilizing whole microorganisms immobilized by the PVA-freezing method. Water Science and Technology 26:1037-1046. 8. Aslan, S., and M. Dahab. 2008. Nitritation and denitritation of ammonium-rich wastewater using fluidized-bed biofilm reactors. Journal of Hazardous Materials 156:56-63. 9. Barnes, C. 1934. Diffusion through a membrane, vol. Physics 5. 10. Bernet, N., and R. Moletta. 2001. Nitrification at low oxygen concentration in biofilm reactor. Journal of Environmental Engineering 127:266-271. 11. Bertanza, G. 1997. Simultaneous nitrification-denitrification process in extended aeration plants: pilot and real scale experience. Water Science and Technology 35:53-61. 12. Bickerstaff, G. F. 1997. Immobilized enzyme and cells, vol. 1. Humana Press Inc., Totowa, N.J., U.S.A. 13. Blackburne, R., and Z. Yuan. 2008. Partial nitrification to nitrite using low dissolved oxygen concentration as the main selection factor. Biodegradation 19:303-312. 14. Chang, C. C., S. K. Tseng, and H. K. Huang. 1999. Hydrogenotrophic denitrification with immobilized Alcaligenes eutrophus for drinking water treatment. Bioresource Technology 69:53-58. 15. Chen, K. C., S. J. Chen, and J. Y. Houng. 1996. Improvement of gas permeability of denitrifying PVA gel beads. Enzyme Microbiological Technology 18:502-506. 16. Chui, P. C., Y. Terashima, and J. H. Tay. 1999. Presented at the IAWQ Conference. 17. Ciudad, G., O. Rubilar, P. Munoz, G. Ruiz, R. Chamy, C. Vergara, and D. Jeison. 2005. Partial nitrification of high ammonia concentration wastewater as a part of a shortcut biological nitrogen removal process. Process Biochemistry 40:1715-1719. 18. Daims, H., A. Bruhl, R. Amann, K. H. Schleifer, and M. Wagner. 1999. The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: Development and evaluation of a more comprehensive probe set. Systematic and Applied Microbiology 22:434-444. 19. Daims, J. W., N. B. Ramsing, K. H. Schleifer, and M. Wagner. 2001. Cultivation-independent, semiautomatic determination of absolute bacterial cell numbers in environmental samples by fluorescence in situ hybridization. Applied and Environmental Microbiology 67:5810-5818. 20. Dalsgaard, T., and B. Thamdrup. 2002. Factors controlling anaerobic ammonium oxidation with nitrite in marine sediments. Applied and Environment Microbiology 68:3802-3808. 21. Dapena-Mora, A., J. L. Campos, A. Mosquera-Corral, M. S. M. Jetten, and R. Mendez. 2004. Stability of the ANAMMOX process in a gas-lift reactor and a SBR. Journal of Biotechnology 110:159-170. 22. DeLong, E. F., G. S. Wickham, and N. R. Pace. 1989. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells, p. 1360-1363, Science, vol. 243. American Association for the Advancement of Science. 23. Dexiang, L., L. Xiaoming, Y. Qi, Z. Guangming, G. Liang, and Y. Xiu. 2007. Effect of inorganic carbon on anaerobic ammonium oxidation enriched in sequencing batch reactor. Journal of Environmental Sciences 20:940–944. 24. Dosta, J., I. Fernandez, J. R. Vazquez-Padin, A. Mosquera-Corral, J. L. Campos, J. Mata-Alvarez, and R. Mendez. 2008. Short- and long-term effects of temperature on the Anammox process. Journal of Hazardous Materials 154:688–693. 25. Egli, K., U. Fanger, P. J. J. Alvarez, H. Siegrist, J. R. van der Meer, and A. J. B. Zehnder. 2001. Enrichment and characterization of an anammox bacterium from a rotating biological contactor treating ammonium-rich leachate. Archaeological Microbiology 175:198-207. 26. Ferris, M. J., G. Muyzer, and D. M. Ward. 1996 Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Applied and Environmental Microbiology 62:340-346. 27. Fick, A. 1855. Poggendorff's Annel, vol. 94. Physik. 28. Fux, C., S. Velten, V. Carozzi, D. Solley, and J. Keller. 2006. Efficient and stable nitritation and denitritation of ammonium-rich sludge dewatering liquor using an SBR with continuous loading. Water Research 40:2765-2775. 29. Gali, A., J. Dosta, M. C. M. van Loosdrecht, and J. Mata-Alvarez. 2007. Two ways to achieve an anammox influent from real reject water treatment at lab-scale: Partial SBR nitrification and SHARON process. Process Biochemistry 42:715-720. 30. Greenberg, A. E., L. S. Clesceri, and A. D. Eaton. 1992. Standard methods for the examination of water and wastewater, vol. 18. American public health association, Washington D.C. 31. Guven, D., A. Dapena, B. Kartal, M. C. Schmid, B. Maas, K. van de Pas-Schoonen, S. Sozen, R. Mendez, H. J. M. Op den Camp, M. S. M. Jetten, M. Strous, and I. Schmidt. 2005. Propionate Oxidation by and Methanol Inhibition of Anaerobic Ammonium-Oxidizing Bacteria. Appl. Environ. Microbiol. 71:1066-1071. 32. Hanaki, K., Hirunmasuwan, S., and Matsuo, T. 1994. Protection of methanogenic bacteria from low pH and toxic materials by immobilization using polyvinyl alcohol. Water Research 28:877-885. 33. Hanaki, K., C. Wantawin, and S. Ohgaki. 1990. Nitrification at low levels of dissolved oxygen with and without organic loading in a suspended-growth reactor. Water Research 24:297-302. 34. Hellinga, C., A. A. J. C. Schellen, J. W. Mulder, M. C. M. van Loosdrecht, and J. J. Heijnen. 1998. The sharon process: An innovative method for nitrogen removal from ammonium-rich waste water. Water Science and Technology 37:135-142. 35. Hendrikus, J. L., and G. Saskia. 1993. Competition for limiting amounts of oxygen between Nitrosomonas europaea and Nitrobacter winogradskyi grown in mixed continuous cultures. Archives of Microbiology V159:453-459. 36. Hunik, J. H. 1993. Engineering aspects of nitrification with immobilized cell. PhD Thesis. Wageningen Agricultural University, Netherlands. 37. Jetten, M. S. M., Wagner, M., Fuerst, J., van Loosdrecht, M., Kuenen, G., and Strous, M. 2001. Microbiology and application of the anaerobic ammonium oxidation ('anammox') process. Current Opinion in Biotechnology 12:283-288. 38. Jetten, M. S. M., S. J. Horn, and M. C. M. van Loosdrecht. 1997. Towards a more sustainable municipal wastewater treatment system. Water Science and Technology 35:171. 39. Jetten, M. S. M., M. Strous, K. T. van de Pas-Schoonen, J. Schalk, U. G. J. M. van Dongen, and A. A. van de Graaf. 1999. The anaerobic oxidation of ammonium. FEMS Microbiology Review 22:421-437. 40. Juretschko, S., G. Timmermann, M. Schmid, K. H. Schleifer, A. Pommereningroser, and H. P. Koops. 1998. Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge-Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations. Applied and Environmental Microbiology 64:3042-3051. 41. Kalmbach, S., W. Manz, B. Bendinger, and U. Szewzyk. 2000. In situ probing reveals Aquabacterium commune as a widespread and highly abundant bacterial species in drinking water biofilms. Water Research 34:575-581. 42. Kalmbach, S., W. Manz, and U. Szewzyk. 1997. Isolation of new bacterial species from drinking water biofilms and proof of their in situ dominance with highly specific 16s rRNA probes. Applied and Environmental Microbiology 63:4164-4170. 43. Kartal, B., J. Rattray, L. A. van Niftrik, J. van de Vossenberg, M. C. Schmid, R. I. Webb, S. Schouten, J. A. Fuerst, J. S. Damste, M. S. M. Jetten, and M. Strous. 2007. Candidatus “Anammoxoglobus propionicus” a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria. Systematic and Applied Microbiology 30:39–49. 44. Kartal, B., L. van Niftrik, O. Sliekers, M. C. Schmid, I. Schmidt, K. van de Pas-Schoonen, I. Cirpus, W. van der Star, M. van Loosdrecht, W. Abma, J. G. Kuenen, J. Mulder, M. S. M. Jetten, H. op den Camp, M. Strous, and J. van de Vossenberg. 2004. Application, eco-physiology and biodiversity of anaerobic ammonium oxidizing bacteria. Reviews in Environmental Science and Biotechnology 3:255–264. 45. Kawaharasaki, M., H. Tanaka, T. Kanagawa, and K. Nakamura. 1999. In situ identification of polyphosphate-accumulatiing bacteria in activated sludge by dual staining with rRNA-targeted oligonucleotide probes and 4',6-diamidino-2-phenylindole (DAPI) at a polyphosphate-probing concentration. Water Research 33:257-265. 46. Keller, G. H., and M. M. Manak. 1989. DNA probes. Stockton press, New York, U.S.A. 47. Kennedy, J. F., E. H. Melo, and K. Jumel. 1990. Chemical Engineering Process. Taylor & Francis, U.K. 48. Kikuchi, T., T. Saito, and T. K. 1999. Presented at the IAWQ Conference. 49. Kuenen, J. G., and M. S. M. Jetten. 2001. Extraordinary anaerobic ammonium-oxidizing bacteria. ASM News 67:456-463. 50. Kuypers, M. M. M., A. O. Sliekers, G. Lavik, M. Schmid, B. B. Jorgensen, J. G. Kuenen, J. S. Sinninghe Damste, M. Strous, and M. S. M. Jetten. 2003. Anaerobic ammonium oxidation by anammox bacteria in the Black Sea. Nature 422:608-611. 51. Lange, J. L., P. S. Thorne, and N. Lynch. 1997. Application of flow cytometry and fluorescent in situ hybridization for assessment of exposures to airborne bacteria. Applied and Environmental Microbiology 63:1557-1563. 52. Lewandowski, Z., R. Bakke, and W. G. Characklis. 1987. Nitrification and autotrophic denitrification in calcium alginate beads. Water Science and Technology 19:175-182. 53. Lindsay, M. R., R. I. Webb, M. Strous, M. S. M. Jetten, M. K. Butler, R. J. Forde, and J. A. Fuerst. 2001. Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell. Archives of microbiology 413-429. 54. Lu, C. J., C. M. Lee, and C. Z. Huang. 1996. Biodegradation of chlorophenols by immobilized pure-culture microorganisms. Water Science and Technology 34:67-72. 55. Macdonald, R., and V. S. Broezel. 2000. Community analysis of bacterial biofilms in a simulated recirculating cooling-water system by fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes. Water Research 34:2439-2446. 56. Manser, R., K. Muche, W. Gujer, and Siegrist. 2005. Arapid method to quantify nitrifiers in activated sludge. Water Research 39:1585-1593. 57. Manz, W. 1999. In situ analysis of microbial biofilms by rRNA-targeted oigonucleotide probing. Methods in Enzymology 310:79-91. 58. Manz, W., U. Szewzyk, P. Ericsson, R. Amann, K. H. Schleifer, and T. A. Stenstrom. 1993. In situ identification of bacteria in drinking water and adjoining biofilms by hybridization with 16S and 23S rRNA-directed fluorescent oligonucleotide probes. Applied and Environmental Microbiology 59:2293-2298. 59. Meinkoth, J., and G. Wahl. 1984. Hybridization of nucleic acids immobilized on solid supports. Analytical Biochemistry 138:267-284. 60. Mobarry, B. K., M. Wagner, V. Urbain, B. E. Rittmann, and D. A. Stahl. 1996. Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. Applied and Environmental Microbiology 62:2156-2162. 61. Moench, T. R., H. E. Gendelman, J. E. Clements, O. Narayan, and D. E. Griffin. 1985. Efficiency of in situ hybridization as a function of probe size and fixation technique. Journal of Virological Methods 11:119-130. 62. Moter, A., and U. B. Gobel. 2000. Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. Journal of Microbiological Methods 41:85-112. 63. Mulder, A., A. A. van de Graaf, L. A. Robertson, and J. G. Kuenen. 1995. Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor. FEMS Microbiol. Ecol. 16:177-184. 64. Nishimura, F. 1999. Presented at the IAWQ Conference. 65. Pardue, M. L. 1985. In situ hybridization, p. 179-202. In B. D. a. S. J. H. Hames (ed.), Nucleic acid hybridization. IRL Press, U.K. 66. Quan, X. C., H. C. Shi, and J. L. Wang. 2003. Biodegradation of 2,4-dichlorophenol in sequencing batch reactors augmented with immobilized mixed culture. Chemosphere 50:1069-1074. 67. Quan, Z., Z. Rhee, J. Zuo, Y. Yang, J. Bae, R. Park, S. Lee, and Y. Park. 2008. Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammoniumoxidizing (anammox) reactor. Environmental Microbiology 10:3130–3139. 68. Rittmann, B. E., and P. L. McCarty. 2001. Environmental Biotechnology: Principles and Applications. 69. Ruiz, G., D. Jeison, and R. Chamy. 2003. Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration. Water Research 37:1371-1377. 70. Rysgaard, S., R. N. Glud, N. Risgaard-Petersen, and D. Dalsgaard. 2004. Denitrification and anammox activity in arctic marine sediments. Limnology & Oceanography 49:1493–1502. 71. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning, vol. 2. Cold Spring Harbor Laboratory Press, U.S.A. 72. Sayavedra-Soto, L. A., N. G. Hommes, and D. J. Arp. 1994. Characterization of the gene encoding hydroxylamine oxidoreductase in Nitrosomonas europaea. Journal of Bacteriology 176:504-510. 73. Schmid, M., U. Twachtmann, M. Klein, M. Strous, S. Juretschko, M. Jetten, J. W. Metzger, K. H. Schleifer, and M. Wagner. 2000. Molecular evidence for genus level diversity of bacteria capable of catalyzing anaerobic ammonium oxidation. Systematic and Applied Microbiology 23:93-106. 74. Schmid, M., K. Walsh, R. Webb, I. C. W. Rijpstra, K. Schonen van de pas, J. M. Verbruggen, T. Hill, B. Moffet, J. Fuerst, S. Schouten, S. S. J. Damste, J. Harris, P. Shaw, M. Jetten, and M. Strous. 2003. Two new species of anaerobic ammonium oxidizing bacteria. Systematic and Applied Microbiology In press. 75. Schmid, M. C., B. Maas, A. Dapena, K. van de Pas-Schoonen, J. van de Vossenberg, B. Kartal, L. van Niftrik, I. Schmidt, I. Cirpus, R. Mendez, M. S. M. Jetten, and M. Strous. 2005. Biomarkers for in situ detection of anaerobic ammonium-oxidizing (anammox) bacteria. Applied and Environment Microbiology 71:1677–1684. 76. Schmidt, I., and E. Bock. 1997. Anaerobic ammonia oxidation with nitrogen dioxide by Nitrosomonas eutropha. Archaeological Microbiology 167:106-111. 77. Schmidt, I., O. Sliekers, M. Schmid, and E. Bock. 2003. New concept of microbial treatment processes for the nitrogen removal in wastewater. FEMS Microbiology Review 27:481-492. 78. Sliekers, O. A., Third, K. A., Abma, W., Kuenen, G. J., and Jetten, M. S. M. 2003. CANON and anammox in a gas-lift reactor. FEMS Microbiology Letters 218:339-344. 79. Stephenson, T., S. Judd, B. Jefferson, and K. Brindle. 2000. Membrane bioreactors for wastewater treatment. IWA, London, U.K. 80. Strous, M., Kuenen, J. G., and Jetten, M. S. M. 1999. Key physiology of anaerobic ammonium oxidation. Applied and Environmental Microbiology 65:2348-2350. 81. Strous, M., E. Gerven, J. G. Kuenen, and M. S. M. Jetten. 1997. Effects of aerobic and microaerobic conditions on anaerobic ammonium-oxidizing (anammox) sludge. Applied and Environmental microbiology 63:2446-2448. 82. Strous, M., J. J. Heijnen, J. G. Kuenen, and M. S. M. Jetten. 1998. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms. Applied Microbiology and Biotechnology 50:589-596. 83. Third, K. A., J. Paxman, M. Schmid, M. Strous, M. S. M. Jetten, and R. Cord-Ruwisch. 2005. Enrichment of Anammox from Activated Sludge and Its Application in the CANON Process Microbial Ecology 49:236-244. 84. Third, K. A., O. A. Sliekers, G. J. Kuenen, and M. S. M. Jetten. 2001. The CANON system (completely autotrophic nitrogen-removal over nitrite) under ammonium limitation:interaction and competition between three groups of bacteria. Systematic and Applied Microbiology 24:588-596. 85. Travieso, L., F. Benitez, P. Weiland, E. Sanchez, R. Dupeyron, and Dominguez. 1996. Experiments on immobilization of microalage for nutrient removal in wastewater treatment. Bioresource Technology 64:884-889. 86. Tsubone, T., Y. Ogaki, Y. Yoshiy, and M. Takahashi. 1996. Effects of biomass entrapment and carrier properties on the performance of an airfluidized-bed biofilm reactor. Water Environmental Research 64:884-889. 87. Tsuneda, S., T. Nagano, T. Hoshino, Y. Ejiri, N. Noda, and A. Hirata. 2003. Characterization of nitrifying granules produced in an aerobic upflow fluidized bed reactor. Water Research 37:4965-4973. 88. Uemoto, H., and A. Saiki. 2000. Distribution of Nitrosomonas europaea and Paracoccus denitrificans immobilized in tubular polymeric gel for nitrogen removal. Applied and Environmental Microbiology 66:816-819. 89. van de Graaf, A. A., P. De Bruijn, L. A. Robertson, M. S. M. Jetten, and J. G. Kuenen. 1996. Autotrophic growth of anaerobic ammonium-oxidizing microorganisms in a fluidized bed reactor. Microbiology 142:2187-2196. 90. van Dongen, U., M. S. M. Jetten, and M. C. van Loosdrecht. 2001. The SHARON-ANAMMOX process for treatment of ammonium rich wastewater. Water Science and Technology 44:153-160. 91. van Niftrik, L. A., J. A. Fuerst, J. S. S. Damste, J. G. Kuenen, M. S. M. Jetten, and M. Strous. 2004. The anammoxosome: an intracytoplasmic compartment in anammox bacteria. FEMS Microbiology Letters 233:7-13. 92. Wagner, M., Rath, G., Koops, H.P., Flood, J. and Amann, R. 1996. In situ analysis of nitrifying bacteria in sewage treatment plants. Water Science and Technology 34:237-244. 93. Wagner, M., G. Rath, H. P. Koops, J. Flood, and T. A. Ali. 1996. Probing activated sludge with oligonucleotides specific for Proteobacteria:inadequacy of culture-dependent methods for describing microbial community structure. Applied and Environmental Microbiology 59:1520-1525. 94. Wallner, G., R. Erhart, and R. Amann. 1995. Flow cytometric analysis of activated sludge with rRNA-targeted probes. Applied and Environmental Microbiology 61:1859-1866. 95. Wang, J. L., L. P. Han, and H. C. Shi. 2001. Biodegradation of quinoline by gel immobilized Burkholderia sp. Chemosphere 44:1041-1046. 96. Wang, J. L., W. H. Hou, and Y. Qian. 1995. Immobilization of microbial cells using polyvinyl alcohol (PVA)-polyacrylamide gels. Biotechnology Techniques 10:203-208. 97. Wang, J. L., P. Liu, and Y. Qian. 1997b. Biodegradation of phthlic acid esters by immobilized microbial cells. Environmental International 23:775-782. 98. Wang, J. L., X. C. Quan, and L. P. Han. 2002a. Microbial degradation of quinoline by immobilized cells of Burkholderia pickettii. Water Research 36:288-296. 99. Wang, J. L., H. C. Shi, and Y. Qian. 1997a. Immobilization of microorganisms using carrageenan gels coated with chitosan and application to biodegradation of 4-chlorophenol. Journal of Environment Science 9:283-287. 100. Wang, J. L., Y. C. Ye, and W. Z. Wu. 2003. Comparison of biodegradation of di-n-methyl phthalate by free and immobilized microbial cells. Biomedical and Environmental Science 16:126-132. 101. Wijffels, R. H. 2000. Immobilized cells. Springer, Wageningen, Netherlands. 102. Windey, K., I. De Bo, and W. Verstraete. 2005. Verstraete, Oxygen-limited autotrophic nitrification–denitrification (OLAND) in a rotating biological contactor treating high-salinity wastewater. 39:4512–4520. 103. Woebken, D., P. Lam, M. M. M. Kuypers, S. W. A. Naqvi, B. Kartal, M. Strous, M. S. M. Jetten, B. M. Fuchs, and R. Amann. 2008. A microdiversity study of anammox bacteria reveals a novel Candidatus Scalindua phylotype in marine oxygen minimum zones. Environmental Microbiology 10:3106–3119. 104. Wu, K. A., and K. D. Wisecarver. 1990. Presented at the AICHE Symposium Series San Francisco, C.A., U.S.A.,. 105. Zhang, Y. M., B. E. Rittmann, and J. L. Wang. 2005. High carbohydrate wastewater treatment by IAL-CHS with immobilized Candida tropicalis. Process Biochemistry 40:857-863. 106. Zhao, H. W., D. S. Mavnic, W. K. Oldham, and F. A. Koch. 1999. Controlling factors for simultaneous nitrification and denitrification in two stage intermittent aeration process treating domestic sewage. Water Research 133:961-970. 107. 陳國誠. 1992. 微生物酵素工程學. 藝軒圖書出版社, 台北, 台灣. 108. 謝淵琳,蔡慧穎,張裕釧,林畢修平. 2004. 本土性含氨氮無氧氧化微生物之混合族群在廢水除氮上的應用. 中華民國環境保護學會學刊 27:218-231. 蘇慧慈. 1995. 原位分子生物學技術. 財團法人徐氏基金會, 台北, 台灣. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16140 | - |
dc.description.abstract | 傳統之生物除氮程序包括自營好氧硝化及異營無氧脫硝兩個階段,此程序需要較大的土地面積,操作上亦較為複雜,且會有殘留碳源之後續處理問題。當前除氮技術的發展傾向利用自營性除氮程序,於單槽內結合部分硝化及厭氧氨氧化之完全自營性生物除氮程序,其具有省空間、可減少氧氣供應量及不需外加碳源等優點,極適合用來處理含低濃度有機碳之高濃度氨氮廢水。但參與該反應之厭氧氨氧化菌,是一種生長速率極為緩慢,且在有氧的環境下會對其反應造成抑制的作用,是為一種非常不易培養的自營菌株。所以,如何將此菌株保留在反應槽中,並維持系統穩定性且同時兼顧實際應用的可行性,遂成為該系統有待克服的一項關鍵性問題
起初,本研究希望利用固定化技術將硝化菌及厭氧氨氧化菌分別予以固定化後,置於一個反應槽中並同時進行生物除氮作用。但固定化厭氧氨氧化菌不論經過何種成形方式,以及長時間的活化過程,其實驗結果皆未觀察出厭氧氨氧化之反應。隨後,藉著生死染色技術的結果證實,絕大多數的厭氧氨氧化菌會在固定化成形過程中遭受到不可恢復性的傷害,乃至於固定化厭氧氨氧化菌失去活性所致。 為突破厭氧氨氧化菌在固定化程序失去活性的困境,本研究嘗試開發出Cookies生物除氮反應系統,將具有一定厚度框架之左、右兩側利用不含硝化菌的人工薄膜包覆,而形成一個中空四立方體之生物除氮模組,並在此中空部分直接注入富含厭氧氨氧化菌之活性污泥。由連續流的實驗結果證實,Cookies生物除氮系統可以長時間操作在高亞硝酸鹽濃度(600 mg-N/L)的環境下,且不會對厭氧氨氧化菌造成不可恢復的危害,惟Cookies生物除氮系統必需操作在低溶氧的環境下才能順利運作,否則在經過一段時間的操作後,厭氧氨氧化反應仍然還是會受到氧氣的抑制而停止。 為改良Cookies生物除氮反應系統在使用上的限制,本研究進一步在人工薄膜中包埋硝化菌株,因而成功開發出一個名為「AOB-Cookies」的新型生物除氮系統。此系統不僅能在單一反應槽中同時完成部分硝化及厭氧氨氧化作用,還能操作在高溶氧的環境下(2 mg/L),仍可確保完全部分硝化作用的順利進行,且過程中不會產生氧氣對厭氧氨氧化菌的抑制作用。當初始氨氮濃度介於30至720 mg/L,AOB-Cookies生物除氮系統皆能達到良好的氨氮去除率,經計算後得比氨氮去除速率各為0.034、0.053、0.056和0.069 mg-N/mg-MLSS/day。利用螢光原位雜交技術進行菌相分析,得知厭氧氨氧化菌分別在AOB-Cookies生物除氮系統中所占比例約為76.1%、氨氧化菌約占19.7%、亞硝酸鹽氧化菌約為2.3%。此外,也由於部分硝化效率的提高,所產生的大量亞硝酸鹽可供厭氧氨氧化反應使用,卻也大幅縮短除氮過程所需時間,整個反應在25個小時以內即可完成。最後,系統中部分硝化反應產生的酸度與厭氧氨氧化反應產生的鹼度相互中和,還能減少酸劑或鹼劑的添加量,進而降低操作成本,因此AOB-Cookies生物除氮系統極具實際應用的潛力及操作彈性。 | zh_TW |
dc.description.abstract | The conventional process for biological removal of nitrogen involves two steps: autotrophic nitrification and heterotrophic anaerobic denitrification. This process requires large land areas. The operation is also more complex and creates subsequent processing problems involving residual carbon sources. Current developmental trends in nitrogen removal technology use the autotrophic nitrogen removal processes. The complete autographic biological nitrogen removal process combines partial nitrification and anaerobic ammonium oxidation in single reactors. This process saves space, reduces oxygen supply, and does not require external carbon sources. The process is suitable for processing high concentrations of ammonia nitrogen wastewater with low concentrations of organic carbon. However, the anammox bacteria taking part in this reaction are an autotrophic strain with a slow growth rate and will be inhibited in aerobic environments. It is also extremely difficult to cultivate. Therefore, preserving this strain in the reactor and maintaining system stability while considering the feasibility of practical application has become a key problem of this system to be overcome.
First, we anticipated using the immobilization technology to immobilize the nitrifying bacteria and the anammox bacteria, which are then placed in a reactor for biological nitrogen removal. However, the experimental results for immobilized anammox bacteria with various shaping methods and long activation processes indicated that anammox reactions did not occur. The results of life and death dyeing technology empirically establish that the vast majority of anammox bacteria were irreversibly harmed in the immobilization forming process, to the point that the stabilized anammox bacteria lose activity. To overcome this loss of activity of anammox bacteria during the immobilization process, we developed the Cookies biological nitrogen-removal reaction system. This system had left and right sides with frameworks of specific thickness that was coated with an artificial thin-film without nitrifying bacteria. This formed a biological nitrogen-removal module with four hollow cubes. Activated sludge rich in anammox bacteria was poured directly into these hollow areas. Continuous flow experiments indicated that the Cookies biological nitrogen-removal system could operate for long periods in environments with high concentrations of nitrite (600 mg-N/L) with no adverse effects to anammox bacteria. However, the Cookies biological nitrogen-removal system must operate in environments with low amounts of dissolved oxygen to be successful. If not, the anaerobic ammonium oxidation reaction is will be inhibited and stopped by oxygen after a period of operation. To correct these limitation in the Cookies biological nitrogen-removal reaction system, we further entrapped nitrifying strains in the artificial film and developed a new biological nitrogen-removal system called AOB-Cookies. This system can perform both partial nitrification and anaerobic ammonium oxidation in a single reactor. Additionally, it can also operate in environments with significant amounts of dissolved oxygen (2 mg/L), while still ensuring the successful partial nitrification. Oxygen does not inhibit the anammox bacterium during this process. When initial ammonia concentrations were between 30 to 720 mg-N/l, the AOB-Cookies biological nitrogen-removal system could achieve excellent ammonia removal rates. Our calculation indicates the ammonia removal rates of 0.034, 0.053, 0.056, and 0.069 mg-N/mg-MLSS/d. We employed fluorescence in situ hybridization techniques for microbial community analysis. This analysis showed that the ratio of anammox bacteria in the AOB-Cookies biological nitrogen-removal system was approximately 76.1%, the ratio of ammonia oxidation bacteria was approximately 19.7%, and the ratio of nitrite oxidation bacteria was approximately 2.3%. In addition, the large amounts of nitrite produced due to the increase in partial nitrification efficiency can be used for anaerobic ammonium oxidation reactions. This also substantially reduces the time required by the nitrogen removal process. The entire reaction can be completed within 25 h. Finally, the acidity produced by partial nitrification and the alkalinity produced by anaerobic ammonium oxidation in the system neutralize each other. This can also reduce the amount of acid or alkali agents needed, thereby reducing the operating costs. Therefore, the AOB-Cookies biological nitrogen-removal system has potential for practical application and provides operating flexibility. | en |
dc.description.provenance | Made available in DSpace on 2021-06-07T18:02:33Z (GMT). No. of bitstreams: 1 ntu-101-D94541002-1.pdf: 31719816 bytes, checksum: a7f4b7991188838983179129fa71977a (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 第一章
前言 ...........................................................................................................................................1 1-1研究緣起 .................................................................................................................................1 1-2研究目的 .................................................................................................................................2 第二章 文獻回顧 ..................................................................................................................................4 2-1生物除氮之基本原理 .........................................................................................................4 2-1-1硝化作用 .....................................................................................................................4 2-1-2脫硝作用 .....................................................................................................................6 2-1-3自營脫硝 .....................................................................................................................6 2-2近年來生物除氮程序之發展趨勢 .................................................................................8 2-3微生物固定化技術及應用 .............................................................................................14 2-4分子生物技術分析微生物菌群結構 ..........................................................................20 第三章 固定化技術於部分硝化作用之研究 ...........................................................................26 3-1材料與方法 ..........................................................................................................................26 3-1-1氨氧化菌之馴養 ....................................................................................................26 3-1-2固定化細胞之製作 ................................................................................................27 3-1-3批次反應槽之設計 ................................................................................................27 3-1-4富含氨氧化菌活性污泥之批次實驗 ..............................................................28 3-1-5固定化富含氨氧化菌細胞之批次試驗.........................................................29 3-1-6水質分析項目及方法 ...........................................................................................30 3-1-7固定化細胞中菌相之分佈與鑑定 ...................................................................30 3-2結果與討論 ..........................................................................................................................33 3-2-1氨氧化菌之馴養結果 ...........................................................................................33 3-2-2改變氨氮濃度對富含氨氧化菌活性污泥之影響結果 ............................34 3-2-3改變溶氧濃度對富含氨氧化菌活性污泥之影響結果 ............................36 3-2-4改變氨氮濃度對固定化氨氧化菌之影響結果 ...........................................37 3-2-5改變溶氧濃度對固定化氨氧化菌之影響結果 ...........................................40 3-2-6富含氨氧化菌活性污泥及固定化氨氧化菌之菌相分析結果..............43 3-3 小結論 ....................................................................................................................................45 第四章 固定化技術於厭氧氨氧化程序之研究 ......................................................................46 4-1材料與方法 ..........................................................................................................................46 4-1-1厭氧氨氧化菌之馴養 ...........................................................................................46 4-1-2固定化細胞之製作 ................................................................................................47 4-1-3批次反應槽之設計 ................................................................................................47 4-1-4富含氨氧化菌活性污泥之批次實驗 ..............................................................47 4-1-5水質分析項目及方法 ...........................................................................................48 4-1-6固定化厭氧氨氧化菌之生死染色法 ..............................................................48 4-1-7固定化厭氧氨氧化菌中菌相之分佈與鑑定 ................................................49 4-2結果與討論 ..........................................................................................................................50 4-2-1厭氧氨氧化菌之馴養結果 .................................................................................50 4-2-2改變氨氮及亞硝酸鹽氮濃度對富含厭氧氨氧化活性污泥之影響結果 ............................................................................................................................................51 4-2-3改變氨氮及亞硝酸鹽氮比例對富含厭氧氨氧化活性污泥之影響結果 ............................................................................................................................................54 4-2-4固定化厭氧氨氧化菌之活化結果 ...................................................................56 4-2-5富含厭氧氨氧化菌活性污泥及固定化厭氧氨氧化菌之菌相分析結果 ............................................................................................................................................58 4-2-6固定化厭氧氨氧化菌之生死染色結果 .........................................................61 4-3小結論 ....................................................................................................................................64 第五章 Cookies和AOB-Cookies生物除氮系統之研發 ..........................................................65 5-1材料與方法 ..........................................................................................................................65 5-1-1人工薄膜之製作 ....................................................................................................65 5-1-2Cookies生物除氮系統之薄膜通透性實驗 ....................................................66 5-1-3生物除氮反應槽之設計 ......................................................................................68 5-1-4Cookies生物除氮系統之批次實驗 ..................................................................69 5-1-5Cookies生物除氮系統之連續流實驗 .............................................................70 5-1-6AOB-Cookies生物除氮系統之批次實驗 .......................................................70 5-1-7水質分析項目及方法 ...........................................................................................71 5-1-8掃描式電子顯微鏡 ................................................................................................71 5-1-9Cookies和AOB-Cookies生物除氮系統中菌相之分佈與鑑定 ................72 5-2結果與討論 ..........................................................................................................................73 5-2-1Cookies生物除氮系統之薄膜通透性實驗結果 ..........................................73 5-2-2Cookies生物除氮系統之批次實驗結果 ........................................................76 5-2-3Cookies生物除氮系統之連續流實驗結果 ....................................................80 5-2-4掃描式電子顯微鏡觀察結果 ............................................................................82 5-2-5AOB-Cookies生物除氮系統之批次實驗結果 .............................................83 5-2-6Cookies和AOB-Cookies生物除氮系統中菌相之分佈與鑑定結果.......87 5-3小結論 ....................................................................................................................................90 5-3-1Cookies生物除氮系統 ..........................................................................................90 5-3-2AOB-Cookies生物除氮系統 ...............................................................................90 第六章 結論與建議 ...........................................................................................................................92 6-1結論 .........................................................................................................................................92 6-1-1固定化技術於部分硝化作用之應用 ..............................................................92 6-1-2固定化技術於厭氧氨氧化程序之應用 .........................................................92 6-1-3Cookies生物除氮系統之研發 ............................................................................93 6-1-4AOB-Cookies生物除氮系統之研發 .................................................................94 6-2建議 .........................................................................................................................................94 參考文獻 ...............................................................................................................................................95 | |
dc.language.iso | zh-TW | |
dc.title | 結合固定化技術與厭氧氨氧化程序於廢水生物除氮之研究 | zh_TW |
dc.title | Biological Nitrogen Removal from Wastewater by Immobilized cells and ANAMMOX Process | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 博士 | |
dc.contributor.coadvisor | 何俊明 | |
dc.contributor.oralexamcommittee | 吳先琪,童心欣,歐陽嶠暉,盧至人,李志源 | |
dc.subject.keyword | AOB-Cookies生物除氮系統,厭氧氨氧化,部分硝化,螢光原位雜交技術,溶氧, | zh_TW |
dc.subject.keyword | AOB-Cookies system,immobilized,anaerobic ammonium oxidation(ANAMMOX),partial nitrification,fluorescence in situ hybridization(FISH),dissolved oxygen, | en |
dc.relation.page | 103 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2012-08-03 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 環境工程學研究所 | zh_TW |
顯示於系所單位: | 環境工程學研究所 |
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