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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳俊宏(Jiun-Hong Chen) | |
| dc.contributor.author | Yu-Wen Hsieh | en |
| dc.contributor.author | 謝郁文 | zh_TW |
| dc.date.accessioned | 2021-06-07T17:59:17Z | - |
| dc.date.copyright | 2012-08-10 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-09 | |
| dc.identifier.citation | Abdelhaleem, M. (2005) 'RNA helicases: regulators of differentiation', Clin Biochem 38(6): 499-503.
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(2007) 'DjCBC-1, a conserved DEAD box RNA helicase of the RCK/p54/Me31B family, is a component of RNA-protein complexes in planarian stem cells and neurons', Dev Dyn 236(12): 3436-50. Zattara, E. E. and Bely, A. E. (2011) 'Evolution of a novel developmental trajectory: fission is distinct from regeneration in the annelid Pristina leidyi', Evol Dev 13(1): 80-95. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16050 | - |
| dc.description.abstract | 再生是動物組織、器官或個體等受損時，修復並使其回復正常功能的生理現象。再生進行時，細胞分裂及分化來重建組織及 形成器官。在水螅及渦蟲中，成體幹細胞（Neoblast），持續進行細胞分裂或細胞分化以維持個體的大小或成長，並有助於再生的進行。儘管刺絲胞動物或扁形動物已成為再生研究的模式物種，其簡單的體制對於研究特定器官的形成及重建有一定的限制。 Aeolosoma viride 為一淡水生環節動物，有十九個體節、中樞神經系統、消化及排泄系統等器官分化。為瞭解其成體幹細胞在個體維持及再生中扮演的角色，Avipiwi、Aviago3 及 Avivasa 等基因被選殖用以定義及追蹤成體幹細胞。結果顯示，這些基因高量表現在完整個體的尾部無性繁殖區域，同時此區域亦具備高量細胞分裂活性。當再生進行時，不論在前三天的頭部再生中組織（Blastema）或後三天的尾部再生組織中皆可偵測到該基因表現或細胞分裂，顯示成體幹細胞會參與在頭部或尾部的再生。此外，使用九十格雷（Gray, Gy）的伽瑪輻射線（r-irradiation）照射個體後，Avipiwi、Aviago3 及 Avivasa 的基因表現量顯著下降，細胞分裂停止，生殖及再生皆無法進行，表示成體幹細胞對 A. viride 的生殖及再生都是必須的。最後，以 RNA 干擾（RNA interference, RNAi）的方式抑制 Avivasa 表現時，發現再生會受到抑制，且再生中組織的細胞分裂亦受到抑制。綜合上述結果，A. viride 的再生及生殖使用相似的細胞或分子機制，都需要成體幹細胞的參與。 | zh_TW |
| dc.description.abstract | Some animals can regenerate their lost tissues or organs. During regeneration, both Tissue remodeling and organ reformation need cell proliferation and/or differentiation. Neoblasts, one of the somatic stem cells, can proliferate to increase cell number in invertebrates, such as hydra or planarian. Although hydra and planarian are good model animals for regeneration research, their body plans are too primitive to study regeneration of complicated organs. Aeolosoma viride, a fresh water annelid with 19 segments, a central nerve system, nephridia, and a complete digestive system with a pharynx and an anus, presents great regenerative ability. To identify and trace its neoblasts, three marker genes including Avipiwi, Aviago3, and Avivasa have been cloned, and these genes are highly expressed both in the posterior and in the regenerating tissues of A. viride. Moreover, cell proliferation rate is higher at these regions. Neoblast is highly activated during generation, first 3 days of anterior regeneration, and last 3 days of posterior regeneration. The reproductive ability and regeneration process were significantly inhibited after 90 Gy r-irradiation. And neoblast activity was not detected under this dose of irradiation. It indicated that neoblast is essential for reproduction and regeneration. Moreover, by RNA interference (RNAi), Avivasa was confirmed to participate in the proliferation ability of the neoblasts. These data strongly suggested that neoblasts in A. viride participate in both reproduction and regeneration, which share similar cellular or molecular mechanisms. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T17:59:17Z (GMT). No. of bitstreams: 1 ntu-101-R99b41008-1.pdf: 38759481 bytes, checksum: 1ba8b70a4834c6b094b3303f0e11c8f3 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 摘要 iii Abstract iv 1. Introduction 1 1.1. The Concept of Regeneration 1 1.2. The Pluripotent Adult Somatic Stem Cell (Neoblast) 2 1.3. The Neoblast Markers 3 1.3.1. piwi 3 1.3.2. vasa 4 1.4. The Origin of PGC and Neoblast in Invertebrates 5 1.5. RNA Interference (RNAi) 6 1.6. The Various regenerative Model Organisms 7 1.7. Introduction of Aeolosoma viride 9 2. Materials and Methods 11 2.1. Animals 11 2.2. Amputation and Regeneration Assay 11 2.3. Total RNA Extraction 11 2.4. Reverse Transcription (RT) 12 2.5. Gene Cloning 12 2.6. Quantification PCR (qPCR) Analysis 13 2.7. γ-irradiation 14 2.8. Immuno-fluorescence 14 2.9. Synthesis of DIG-labeled Riboprobes or Unlabeled dsRNAs of Avipiwi, Aviago3, or Avivasa 15 2.10. in situ Hybridization 16 2.11.RNA Interference (RNAi) 17 2.12. Statistical Analysis 18 3. Results 19 3.1. Identification of the Homologuous Genes of piwi and vasa in A. viride 19 3.1.1. Avipiwi 19 3.1.2. Aviago3 20 3.1.3. Avivasa 20 3.2. Identification of the Neoblast in A. viride 21 3.3. The Role of Neoblasts in Reproduction of A. viride 22 3.3.1. Cell Proliferation and Gene Expression 22 3.3.2. The Effect of γ-irradiation 23 3.4. The Role of Neoblasts in Anterior Regeneration of A. viride 25 3.4.1. Cell Proliferation 25 3.4.2. Gene Expression 26 3.4.3. The Effect of γ-irradiation 26 3.5. The Role of Neoblasts in Posterior Regeneration of A. viride 28 3.5.1. Cell Proliferation 28 3.5.2. Gene Expression 29 3.5.3. The Effect of γ-irradiation 29 3.6. The Roles of Avivasa in Neoblast 29 4. Discussion 31 4.1. Models for the Neoblast Functions in Reproduction and Regeneration 31 4.2. Comparison of the Strategies Used For Reproduction and Regeneration in A. viride 32 4.3. Comparison of the Reproduction and Regeneration Mechanisms in Various Organisms 34 References 37 Figures 43 | |
| dc.language.iso | zh-TW | |
| dc.subject | piwi | zh_TW |
| dc.subject | 無性繁殖 | zh_TW |
| dc.subject | 成體幹細胞 | zh_TW |
| dc.subject | 細胞分裂 | zh_TW |
| dc.subject | 再生 | zh_TW |
| dc.subject | vasa | zh_TW |
| dc.subject | Aeolosoma viride | zh_TW |
| dc.subject | Cell proliferation | en |
| dc.subject | vasa | en |
| dc.subject | piwi | en |
| dc.subject | Asexual reproduction | en |
| dc.subject | Aeolosoma viride | en |
| dc.subject | Neoblast (Adult somatic stem cell) | en |
| dc.subject | Regeneration | en |
| dc.title | 成體幹細胞在環節動物Aeolosoma viride再生及生殖中扮演的角色 | zh_TW |
| dc.title | The Roles of Neoblasts on Regeneration and Reproduction in the Annelid, Aeolosoma viride | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 朱家瑩(Chia-Ying Chu) | |
| dc.contributor.oralexamcommittee | 李心予(Hsinyu Lee),張俊哲(Chun-Che Chang) | |
| dc.subject.keyword | Aeolosoma viride,成體幹細胞,細胞分裂,再生,無性繁殖,piwi,vasa, | zh_TW |
| dc.subject.keyword | Aeolosoma viride,Neoblast (Adult somatic stem cell),Cell proliferation,Regeneration,Asexual reproduction,piwi,vasa, | en |
| dc.relation.page | 79 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2012-08-09 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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