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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李嗣涔(Si-Chen Lee) | |
| dc.contributor.author | Cheng-Yu Chi | en |
| dc.contributor.author | 紀承諭 | zh_TW |
| dc.date.accessioned | 2021-06-07T17:54:13Z | - |
| dc.date.copyright | 2012-12-10 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-16 | |
| dc.identifier.citation | [1] Alison MR, Poulsom R, Forbes S and Wright NA (2002) An introduction to stem cells. J Pathol 197(4):419-423.
[2] Ariff Bongso, Eng Hin Lee. Stem Cells: From Bench to Bedside. [3] Herzog EL, Chai L and Krause DS (2003) Plasticity of marrow-derived stem cells. Blood 102(10):3483-3493. [4] Revishchin AV, Korochkin LI, Okhotin VE and Pavlova GV (2008) Neural stem cells in the mammalian brain. Int Rev Cytol 265:55-109. [5] Gates CB, Karthikeyan T, Fu F and Huard J (2008) Regenerative medicine for the musculoskeletal system based on muscle-derived stem cells. J Am Acad Orthop Surg 16(2):68-76. [6] Otto WR (2002) Lung epithelial stem cells. J Pathol 197(4):527-535. [7] Zaret KS (2008) Genetic programming of liver and pancreas progenitors: lessons for stem-cell differentiation. Nat Rev Genet 9(5):329-340. [8] Zhang YQ, Kritzik M and Sarvetnick N (2005) Identification and expansion of pancreatic stem/progenitor cells. J Cell Mol Med 9(2):331-344. [9] Benitah SA (2007) Epidermal stem cells in skin homeostasis and cutaneous carcinomas. Clin Transl Oncol 9(12):760-766. [10] Taupin P (2006) OTI-010 Osiris Therapeutics/JCR Pharmaceuticals. Curr Opin Investig Drugs 7(5):473-481. [11] Bez A, Corsini E, Curti D, et al. (December 2003). 'Neurosphere and neurosphere-forming cells: morphological and ultrastructural characterization'. Brain Res. 993 (1–2): 18–29. [12] Reynolds and Weiss, Science 255:1707–1710, 1992. [13] Loic P. Deleyrolle and Brent A. Reynolds. “Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells.” [14] Balasubramaniam, B., D.A. Carter, E.J. Mayer, and A.D. Dick. 2009. Microglia derived IL-6 suppresses neurosphere generation from adult human retinal cell suspensions. Exp. Eye Res. 89:757–766. doi:10.1016/ j.exer.2009.06.019. [15] M. W. Tsai, T. H. Chuang, C. Y. Meng, Y. T. Chang, and S. C. Lee, “High performance midinfrared narrowband plasmonic thermal emitter,” App. Phys. Lett. 89, 173116-173118 (2006). [16] T. H. Chuang, M. W. Tsai, Y. T. Chang, and S. C. Lee, “Remotely coupled surface plasmons in a twocolored plasmonic thermal emitter,” App. Phys. Lett. 89, 173128-173130 (2006). [17] M. U. Pralle, N. Moelders, M. P. McNeal, I. Puscasu, A. C. Greenwald, J. T. Daly, E. A. Johnson, T.George, D. S. Choi, I. El-Kady, and R. Biswas, “Photonic crystal enhanced narrow-band infrared emitters,”Appl. Phys. Lett. 81, 4685-4687 (2002). [18] Singec, I., Knoth, R., Meyer, R. P., et al. (2003) Defining the actual sensitivity and specificity of the neurosphere assay in stem cell biology. Nat. Methods 3, 801–806. [19] R. H. Ritchie, Phys. Rev. 106, 874−881 (1957). [20] H. Raether, Surface Plasmons (Springer-Verlag, Berlin, 1988). [21] C. J. Powell, J. B. Swan, Phys. Rev. 118, 640 (1960). [22] William L. Barnes, Alain Dereux and Thomas W. Ebbesen, Nature (London) 424, 824 (2003). [23] E. Kretschmann and H. Raether, Z. Naturforsch. A 23, 2135-2136 (1968). [24] Yi-Tsung Chang, Tzu-Hung Chuang, Ming-Wei Tsai, Lung-Chien Chen, and Si-Chen Lee. Dispersion relation of Al/Si surface plasmon in hexagonally ordered aluminum hole arrays. Journal of Applied Physics 101, 054305 (2007). [25] Handbook of Instrumental Techniques for Analytical Chemistry, Ch.15, edited by C. P. Sherman Hsu. [26] Yi-Tsung Chang, Yi-Ting Wu, Jeng-Han Lee, Chia-Ming Liang, Chao-Ju Huang and Si-Chen Lee.” Intensity Dependence of (1,0) and (1,1) Ag/SiO2 Surface Plasmons in Ag/SiO2/Ag Plasmonic Thermal Emitter on Energy Distribution of a Graybody Emitter”. 9th Nanotechnology Conference IEEE NANO 2009 Genoa, Italy, July 26-30 2009. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15874 | - |
| dc.description.abstract | 本研究的第一個目的是利用本實驗室所研發出的表面電漿子紅外線發射器，將選定波段(3~5 μm)的寬頻紅外光去照射新生小鼠的腦細胞七天，並利用各種分析方法測量細胞受紅外光激發所造成的反應並探討其在生長及分化的過程中所顯現的生理差異。第二個實驗的研究目的是採用特定波長的窄頻紅外光(3.5,4.0,4.5 μm)照射新生小鼠的腦細胞七天並觀察其在不同波段的生長狀況，以推知小鼠腦細胞對於何種特定波段紅外光最為敏感。本研究當中所使用的紅外光發射器，乃以表面電漿子理論為製程結構設計的主要依據。此紅外光源發射器結構是在矽基板上鍍上一層鉬金屬作為導電電極，接著鍍上銀/二氧化矽/銀三層,即金屬/絕緣層/金屬(MIM)三明治結構的薄膜。最上層的金屬銀表面使用本實驗室所繪製之光罩並利用曝光技術所製作的週期性排列之孔洞以調變實驗所需要的特定波長。藉由鉬金屬通電流加熱，此發射器元件便可發出窄頻寬及高功率的紅外光。在第一個實驗中，由分析結果顯示細胞會受到紅外光誘導而有顯著的增長現象並可聚集成神經幹細胞球。而由代謝體分析可得知照射特定寬頻波段紅外光可使細胞中粒線體的生理狀況傾向於缺氧代謝路徑而較適合其生長。第二個實驗結果顯示窄頻紅外光在4.0μm的的波長下，對細胞的生長促進有最顯著的影響，且可使其生長數量提升45%。綜合以上結果可知照射紅外光後，細胞數量及神經幹細胞球的體積均有所提升，顯示特定波段紅外光照射對細胞生長之促進有益。 | zh_TW |
| dc.description.abstract | The purpose of this research is to investigate the infrared irradiation effect on the cell growth and cell physiology of neonatal mice brain cell expression. The plasmonic thermal emitter with broad bandwidth (3~5 μm) and specific emission waveband were designed and used to irradiate the cells for 7 days in order to identify the specific wavelength that induces the cell expression the most significant. Infrared emitters used in this study are fabricated based on the theory of surface plasmon. The purpose of the second experiment is to understand the effect of narrow band infrared irradiation with 3.5, 4.0, and 4.5 μm wavelengths on cell growth features. The heat is generated by inputting electric current to the molybdenum film on silicon substrate. The infrared source can be achieved by heating the triple layer structure which consists of a SiO2 layer between two Ag films on the molybdenum, it is called metal/dielectric/metal (MIM) structure. The top Ag layer is perforated by periodic holes array, and the emission wavelength can be altered and selected by changing the lattice constant and diameter of the holes arrays. By metabolomics analysis, it is found that the mitochondria in the cell tend to follow the glycolysis pathway which can be benefit to the cell growth. It is observed that after illumination by PTE with peak wavelength at 4.0μm, the cell exhibits the largest response and cell number increased up to 45%. The experiments indicate that the cell number can be enhanced and tend to form neurospheres after infrared illumination. The study shows that IR illumination can improve the cell growth and points to new possibilities for future research. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T17:54:13Z (GMT). No. of bitstreams: 1 ntu-101-R99945036-1.pdf: 5620356 bytes, checksum: ebef6de79d8332e48bc02aff05b1d1cc (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | Chapter 1 Introduction……………………………………………………………………………………1
1.1 Introduction of the Stem Cells……………………………………………1 1.2 The Introduction of the Neurospheres……………………………4 1.3 Objectives and motivations of the Research……………7 1.4 Framework of the Thesis………………………………………………………………10 Chapter 2 The Fundamentals of Plasmonic Thermal Emitters……………………………………………………………………………………………………………………………12 2.1 The Fundamentals of Surface Plasmons……………………………………12 2.1.1 Surface plasmons on smooth surfaces……………………………………12 2.1.2 Surface plasmons on the surface with hole arrays…18 2.1.3 Dispersion relation of surface plasmon in hexagonally ordered hole arrays………………………………………………………………………………………………22 2.2 Process Flow……………………………………………………………………………………………………25 2.2.1 Fabrication processes of metal hole arrays…………………25 2.2.2 Fabrication processes of plasmonic thermal emitter…28 2.3 Measuring Systems………………………………………………………………………………………31 2.3.1 Introduction of FTIR……………………………………………………………………………31 2.3.2 Thermal emitter chamber……………………………………………………………………33 Chapter 3 Experimental and Analysis of Broadband Infrared Irradiation Effect…………………………………………………………………………………………………35 3.1 Experiment Flow……………………………………………………………………………………………36 3.2 Materials and Experimental Process…………………………………………43 3.2.1 Experiment Materials and Reagents…………………………………………43 3.2.2 Neonatal Neurosphere Culture Protocol………………………………43 3.2.3 Cell counting and image processing by using of ImageJ …………………………………………………………………………………………………………………………………………………46 3.2.4 WST Analysis…………………………………………………………………………………………………54 3.2.5 RT-PCR…………………………………………………………………………………………………………………60 3.2.6 Immunocytochemistry (ICC)………………………………………………………………67 3.2.7 Mitochondria Assay of OCR and ECAR Test…………………………70 3.2.8 Metabolomics Biostatistics Analysis……………………………………92 Chapter 4 Expression of Neurosphere under specific Narrowband Infrared Irradiation……………………………………………………………102 4.1 Response Expression of Neurospheres Illuminated by 3.5~4.5μm Infrared Light………………………………………………………………………………102 4.1.1 Infrared exposure experiment #1 at peak wavelength λp= 4 μm…………………………………………………………………………………………………………………………104 4.1.2 Infrared exposure experiment #2 at peak wavelength λp= 4.5 μm……………………………………………………………………………………………………………………114 4.1.3 Infrared exposure experiment #3 at peak wavelength λp= 3.5 μm……………………………………………………………………………………………………………………120 4.2 Discussion………………………………………………………………………………………………………125 Chapter 5 Conclusions and future works………………………………………129 5.1 Conclusions……………………………………………………………………………………………………129 5.2 Future Works…………………………………………………………………………………………………131 Reference ……………………………………………………………………………………………………………………133 | |
| dc.language.iso | en | |
| dc.subject | MIM | zh_TW |
| dc.subject | 神經幹細胞球 | zh_TW |
| dc.subject | 小鼠腦細胞 | zh_TW |
| dc.subject | 窄頻 | zh_TW |
| dc.subject | 表面電漿子紅外線發射器 | zh_TW |
| dc.subject | MIM | en |
| dc.subject | Infrared irradiation | en |
| dc.subject | specific waveband | en |
| dc.subject | neurospheres | en |
| dc.subject | plasmonic thermal emitter | en |
| dc.title | 寬頻及窄頻可選擇特定波段表面電漿子熱紅外光發射器元件照射神經幹細胞球之生理表現的影響探討 | zh_TW |
| dc.title | Effect of Selected Broad and Narrow Bandwidth Plasmonic Thermal Emitter Infrared Radiation on the Neurosphere Cell Features Expression | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林泰元,林世明 | |
| dc.subject.keyword | 表面電漿子紅外線發射器,窄頻,MIM,小鼠腦細胞,神經幹細胞球, | zh_TW |
| dc.subject.keyword | Infrared irradiation,plasmonic thermal emitter,specific waveband,MIM,neurospheres, | en |
| dc.relation.page | 135 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2012-08-17 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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