止水中的懸浮磁性粒子之多型態非實體通道系統

Abstract

微流道與磁性粒子長期被應用在生物醫學和化學領域，功能包含分子操控、化學混和、細胞分離或者蛋白質的篩選萃取等等，主要原因即是因透過微機電技術製成之晶片成本相較過去實驗低廉許多。然而過去主流的微流道實驗研究都專注於「實體」通道的發展與改善，儘管成本比舊式實驗低廉，但礙於為了侷限住流體，最後過程仍需對晶片加以覆蓋並接合，使溶液的流動會限制於晶片的微流道中流動，覆蓋接合之過程不僅耗時耗本，更可能致使微流道晶片無法長期重複使用，所以「非實體」通道就成為本篇論文的探討方向。本篇論文的重點在於設計多種非實體通道，目的為促使死水溶液中的粒子可依循設計上的通道流動，優點不只不必對晶片加工，在操作方面更是不必透過外加電流、電壓或水流幫浦等，成本比實體通道來的更低廉。 在多次實驗觀察下來，會發現磁性粒子的流動性不只由磁場強度來決定，更重要必須要考量到晶片上鎳材受極化之影響，我們分別分析了不同寬度(體積)的鎳材在外加磁場下對於粒子之影響，同時也分析了不同流道結構於外加強磁下的影響，考量變因有外加磁場方向、流道寬度與流道形狀等等，種種的分析結果都顯示極化現象的考量是未來流道設計之成敗關鍵。

Microfluidic channel systems, which are made from MEMS fabrication, combining with the magnetic beads are widely applied in the biomedical and chemistry fields such as protein screening, cell separation and molecular manipulation. The main reason is due to the lower cost per chip than before. Today’s microfluidic chip is mostly the “real entity” of the microfluidic channel systems. Even thought the cost is lower than before, but it still to seals up the upper cover of the channel to confine the fluidic body at the last step of the fabrication process. That still not good enough because the limitations of use of number of times. So we research about the “virtual” channels. The main point of this paper is research how to design channels to guide the magnetic beads, do not need use pumps, voltage and current. It will be cost down. We find that the moving of magnetic beads not only decide by magnetic fields, but also affected by the polarization of the nickel on the chips. We research the relation of magnetic particles, magnetic fields, and nickel width. And we also research the magnetic beads flow in different channel structures. The direction of magnetic fields, width of channel, and channel shape are both to take consider. The analysis results show that the consideration of polarization is the key to the success of the channel design.