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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15632完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王勝仕(Sheng-Shih Wang) | |
| dc.contributor.author | Jhih- Hao Yang | en |
| dc.contributor.author | 楊智皓 | zh_TW |
| dc.date.accessioned | 2021-06-07T17:49:08Z | - |
| dc.date.copyright | 2020-08-10 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-04 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15632 | - |
| dc.description.abstract | 生物活性化合物對於人體有許多健康上的益處，但是它們在化學性質上並不穩定。由於它們快速釋放、低溶解度以及生物利用度差等性質，因此生物活性化合物在應用上會有所限制。將生物活性化合物包覆能夠抵抗環境的變化或是改善其物理化學性質，而能夠保存生物活性化合物。本研究使用乳清分離蛋白類澱粉纖維以及果膠作為載體，並利用逐層靜電沉積法分別包覆檸檬烯以及葵花油，探討檸檬烯的穩定性以及釋放行為和葵花油之氧化安定性。 結果顯示，在高溫80°c、pH 2以及60 r.p.m攪拌下培養一天生成的乳清分離蛋白類澱粉纖維，由TEM可以看出纖維狀的結構。包覆檸檬烯的系統中，從外觀以及creaming index結果中可以發現以逐層靜電沉積法製備的乳狀液且果膠濃度較高的組別有較好的穩定性。從SEM結果來看，相較於其他組別，LbL(1:0.2)組的表面結構較為平滑完整沒有明顯的破裂以及皺褶。檸檬烯釋放結果以及first-order fitting都顯示LbL(1:0.5)控制釋放的效果最為顯著。此外Korsmeyer-Peppas model fitting結果顯示Fibril組為鬆弛釋放，而LbL組以及Complex組都屬於非費克定律。 葵花油氧化安定性實驗結果顯示，不論是Fibril、Complex以及LbL組別抗氧化效果都比Tween 80小分子非離子型乳化劑來的好。其中Fibril組為抗氧化效果最好，而LbL組隨著果膠量的增加硫代巴比妥酸反應物量也有稍微增加的趨勢。結果的原因推測可能是LbL方式製備下隨著果膠的濃度愈高，負電也就愈多，因此會因為靜電作用力吸引亞鐵離子而提高油被氧化的可能。另一方面Fibril是帶正電會排斥亞鐵離子而減少油被氧化的機會。 | zh_TW |
| dc.description.abstract | Bioactive compounds possess many health benefits but are chemically unstable. The application of bioactive compounds is very limited due to their fast release, low solubility, and poor bioavailability. Encapsulation can protect the bioactive compounds from the detrimental effects of environmental stresses and improve physicochemical functionalities. In this study, whey protein isolate amyloid fibril and pectin were used as carriers and layer-by-layer electrostatic deposition method was used to encapsulate limonene and sunflower oil. We explore the stability and release behavior of limonene and the oxidation stability of sunflower oil. The results from TEM image show that whey protein isolate amyloid fibril incubated at 80°C, pH 2, with stirring speed at 60 r.p.m form fibril structure. Based on the appearance and creaming index results of the encapsulating limonene, the higher pectin concentration emulsion prepared by layer-by-layer electrostatic deposition method has better stability. Based on the SEM image, the surface structure of the LbL (1:0.2) is smooth and intact without obvious cracks and wrinkles compared with other groups. The results of both limonene release and first-order fitting show that LbL (1:0.5) has the most significant effect of controlled release. In addition, the Korsmeyer-Peppas model fitting results show that the fibril group exhibits relaxational release while the LbL group and the complex group belong to non-Fick's law. Sunflower oil oxidation stability experiment results show that fibril, complex and LbL groups have better antioxidant effects than Tween 80, the small molecule nonionic emulsifier. Among these groups, fibril has the best antioxidant effect. The amount of pectin increases, the LbL group has a slight increase in the amount of thiobarbituric acid reactive substances. These results suggest that the higher pectin concentration under the LbL method, have more negative charge, which attracts ferrous ions by electrostatic force and increase the possibility of lipid oxidation. On the other hand, the positively charged fibrils repel ferrous ions so that lipid oxidation is reduce. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T17:49:08Z (GMT). No. of bitstreams: 1 U0001-0308202017580600.pdf: 3219570 bytes, checksum: a3810e01a8648c5de8deb7da5f916198 (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 誌謝 I 摘要 II ABSTRACT III 目錄 V 圖目錄 VIII 表目錄 XI 第一章 緒論 1 1-1研究動機 1 第二章 文獻回顧 3 2-1蛋白質簡介: 3 2-1-1胺基酸: 4 2-1-2蛋白質結構: 5 2-1-3蛋白質變性(Protein Denaturation) 9 2-2類澱粉纖維 9 2-3蛋白質與類澱粉纖維於材料上之特性 11 2-4包覆(Encapsulation) 13 2-5載體(Carrier agents) 15 2-5-1多醣類載體(Polysaccharide-based Carrier agents) 16 2-5-2蛋白質類載體(Protein-based Carrier agents) 26 2-5-3脂質類載體(Lipid-based Carrier agents) 29 2-6微米、奈米包覆技術 30 2-6-1乳化(Emulsification) 30 2-6-2逐層靜電沉積(Layer by Layer electrostatic deposition)(LbL) 32 2-7乳化不穩定機制 36 2-8脂質氧化(Lipid oxidation) 38 2-9檸檬烯(Limonene) 45 2-10實驗原理介紹 46 2-10-1 Thioflavin T螢光光譜: 46 2-10-2 硫代巴比妥酸(Thiobarbituric acid) 47 第三章 實驗儀器、藥品與實驗步驟 48 3-1實驗裝置: 48 3-2實驗藥品: 49 3-3實驗方法及步驟: 50 3-3-1 乳清分離蛋白類澱粉纖維製備(whey protein isolate fibril) [79] 50 3-3-2 乳狀液(emulsion)製備[79] 50 3-3-3 檸檬烯乳狀液(emulsion)定性之檢測[79]: 52 3-3-4 模擬口腔唾液緩衝溶液配置[79] 54 3-3-5 檸檬烯檢量線製備[92] 54 3-3-6檸檬烯釋放[93] 55 3-3-7硫代巴比妥酸(Thiobarbituric acid)檢量線製備[94] 56 3-3-8葵花油氧化安定性實驗[94] 56 第四章 實驗結果與討論 57 4-1檸檬烯乳狀液(emulsion)的性質鑑定與外觀觀察結果: 57 4-1-1 乳清分離蛋白纖維化結果(WPI fibril): 57 4-1-2 檸檬烯乳狀液(emulsion)外觀、粒徑大小及界面電位: 59 4-1-3 檸檬烯乳狀液(emulsion) creaming index 67 4-2 檸檬烯釋放: 69 4-2-1 檸檬烯檢量線結果: 69 4-2-2 檸檬烯釋放結果: 71 4-2-3檸檬烯釋放數學模式fitting[95] 77 4-3葵花油氧化安定性: 81 4-3-1硫代巴比妥酸(Thiobarbituric acid)檢量線結果: 81 4-3-2葵花油氧化之結果: 83 第五章 結論 88 參考文獻 90 | |
| dc.language.iso | zh-TW | |
| dc.subject | 生物活性化合物 | zh_TW |
| dc.subject | 逐層靜電沉積 | zh_TW |
| dc.subject | 檸檬烯 | zh_TW |
| dc.subject | 乳清分離蛋白類澱粉纖維 | zh_TW |
| dc.subject | 果膠 | zh_TW |
| dc.subject | 包覆 | zh_TW |
| dc.subject | 葵花油 | zh_TW |
| dc.subject | sunflower oil | en |
| dc.subject | limonene | en |
| dc.subject | pectin | en |
| dc.subject | whey protein isolate amyloid fibril | en |
| dc.subject | layer-by-layer electrostatic deposition | en |
| dc.subject | encapsulation | en |
| dc.subject | bioactive compounds | en |
| dc.title | 備製與鑑定以乳清分離蛋白類澱粉纖維及果膠所構成之微膠囊包覆系統 | zh_TW |
| dc.title | Preparation and Characterization of the Microcapsules Encapsulation Systems Based on Whey Protein Isolate Amyloid Fibrils and Pectin | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 賴進此(Jin-Cih Lai),侯劭毅(Shao-Yi Hou),吳宛儒(Wan-Ru Wu),林達顯(Da-Sian Lin),蔡伸隆(Shen-Long Cai) | |
| dc.subject.keyword | 生物活性化合物,包覆,逐層靜電沉積,乳清分離蛋白類澱粉纖維,果膠,檸檬烯,葵花油, | zh_TW |
| dc.subject.keyword | bioactive compounds,encapsulation,layer-by-layer electrostatic deposition,whey protein isolate amyloid fibril,pectin,limonene,sunflower oil, | en |
| dc.relation.page | 97 | |
| dc.identifier.doi | 10.6342/NTU202002305 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2020-08-05 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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