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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李伯訓 | |
| dc.contributor.author | Pei-Ju Wu | en |
| dc.contributor.author | 吳沛洳 | zh_TW |
| dc.date.accessioned | 2021-06-07T17:29:19Z | - |
| dc.date.copyright | 2020-03-12 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-02-05 | |
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Hu, Effects of encapsulated propolis on blood glycemic control, lipid metabolism, and insulin resistance in type 2 diabetes mellitus rats, Evid Based Complement Alternat Med 2012 (2012), 981896. 51. L. J. Kang, H. B. Lee, H. J. Bae and S. G. Lee, Antidiabetic effect of propolis: reduction of expression of glucose-6-phosphatase through inhibition of y279 and y216 autophosphorylation of gsk-3alpha/beta in hepg2 cells, Phytother Res 24 (2010), no. 10, 1554-1561. 52. Y. Yoshimasu, T. Ikeda, N. Sakai, A. Yagi, S. Hirayama, Y. Morinaga, S. Furukawa and R. Nakao, Rapid bactericidal action of propolis against porphyromonas gingivalis, J Dent Res 97 (2018), no. 8, 928-936. 53. F. A. Santos, E. M. Bastos, M. Uzeda, M. A. Carvalho, L. M. Farias, E. S. Moreira and F. C. Braga, Antibacterial activity of brazilian propolis and fractions against oral anaerobic bacteria, J Ethnopharmacol 80 (2002), no. 1, 1-7. 54. S. Sonmez, L. Kirilmaz, M. Yucesoy, B. Yucel and B. Yilmaz, The effect of bee propolis on oral pathogens and human gingival fibroblasts, J Ethnopharmacol 102 (2005), no. 3, 371-376. 55. A. Shabbir, M. Rashid and H. N. Tipu, Propolis, a hope for the future in treating resistant periodontal pathogens, Cureus 8 (2016), no. 7, e682. 56. C. A. Dornelas, F. V. Fechine-Jamacaru, I. L. Albuquerque, H. I. F. MagalhãesI, A. J. S. d. Souza, L. A. n. Alves, P. R. C. d. Almeida, T. L. G. d. Lemos, J. D. V. d. Castro, M. E. A. Moraes and M. O. Moraes, Chemoprevention with green propolis green propolis extracted in l- lysine versus carcinogenesis promotion with l-lysine in n-butyl-n-[4-hydroxybutyl] nitrosamine (bbn) induced rat bladder cancer, Acta Cirúrgica Brasileira 27 (2012), no. 2, 185-192. 57. U. Czyzewska, K. Siemionow, I. Zareba and W. Miltyk, Proapoptotic activity of propolis and their components on human tongue squamous cell carcinoma cell line (cal-27), PLoS One 11 (2016), no. 6, e0157091. 58. D. R. R. Cavalcante, P. S. d. Oliveira, S. M. Góis, A. F. Soares, J. C. Cardoso, F. F. Padilha and R. L. C. d. Albuquerque Júnior, Effect of green propolis on oral epithelial dysplasia in rats, Braz J Otorhinolaryngol 77 (2011), no. 3, 278-284. 59. Y. W. Chen, S. W. Wu, K. K. Ho, S. B. Lin, C. Y. Huang and C. N. Chen, Characterisation of Taiwanese propolis collected from different locations and seasons, J Sci Food Agr 88 (2008), no. 3, 412-419. 60. C. Y. Hsieh, L. H. Li, Y. K. Rao, T. C. Ju, Y. S. Nai, Y. W. Chen and K. F. Hua, Mechanistic insight into the attenuation of gouty inflammation by Taiwanese green propolis via inhibition of the nlrp3 inflammasome, J Cell Physiol 234 (2019), no. 4, 4081-4094. 61. L. H. Chen, Y. W. Chien, M. L. Chang, C. C. Hou, C. H. Chan, H. W. Tang and H. Y. Huang, Taiwanese green propolis ethanol extract delays the progression of type 2 diabetes mellitus in rats treated with streptozotocin/high-fat diet, Nutrients 10 (2018), no. 4, 503 62. Y. W. Chen, S. R. Ye, C. Ting and Y. H. Yu, Antibacterial activity of propolins from Taiwanese green propolis, J Food Drug Anal 26 (2018), no. 2, 761-768. 63. H. Y. Yang, C. M. Chang, Y. W. Chen and C. C. Chou, Inhibitory effect of propolis extract on the growth oflisteria monocytogenes and the mutagenicity of 4-nitroquinoline-n-oxide, J Sci Food Agr 86 (2006), no. 6, 937-943. 64. L. C. Lu, Y. W. Chen and C. C. Chou, Antibacterial activity of propolis against staphylococcus aureus, Int J Food Microbiol 102 (2005), no. 2, 213-220. 65. L. C. Lu, Y. W. Chen and C. C. Chou, Antibacterial and DPPH free radical-scavenging activities of the ethanol extract of propolis collected in Taiwan, J Food Drug Anal 11 (2003), no. 4, 277-282. 66. F. M. Suk, G. S. Lien, W. J. Huang, C. N. Chen, S. Y. Lu, Y. C. Yang, M. D. Yan and Y. C. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15283 | - |
| dc.description.abstract | 人類伴隨著科技發展使生活型態與飲食習慣改變，帶來許多對健康造成傷害的疾病，目前齲齒、牙周病與慢性代謝疾病是世界上常見的疾病，治療這些疾病之花費甚巨。隨著人們對身體健康的重視，要如何降低成本與有效預防和治療這些疾病是目前生物醫學界積極地想達成的目標。蜂膠(Propolis)在世界許多地區被證實具有相當多的生物活性且已被當傳統醫療藥物使用，台灣綠蜂膠(Taiwanese green propolis)除了是台灣特有種蜂膠更擁有抗微生物、抗發炎、抗氧化與抗腫瘤等多樣的生物活性，我們想利用擁有多種生物活性的台灣綠蜂膠及其蜂膠素來探討是否可以預防這些疾病的產生或抑制疾病的加劇。
探討台灣綠蜂膠萃取物之多樣的生物活性，實驗設計為利用最低抑菌濃度(MIC)與最低殺菌濃度(MBC)實驗得知蜂膠、蜂膠素及蜂膠素組合分別對會造成齲齒(Dental caries)之突變鏈球菌(S. mutans)與會造成牙周病(Periodontal disease)之牙齦卟啉單胞菌(P. gingivalis)和放線桿菌(A. actinomycetemcomitans)抗菌之活性效果;利用台灣綠蜂膠針對人類胚胎上顎間質細胞(HEPM)進行骨礦化的測定實驗;並模擬口腔實驗運用突變鏈球菌(S. mutans)與大腸桿菌(E. coli)對牛牙進行細菌貼附後，使用顯微鏡觀測與液態培養細菌，得知台灣綠蜂膠抗菌貼附的效果;最後利用台灣綠蜂膠刺激小鼠間質幹細胞(C3H)分化為脂肪細胞的影響。 實驗結果顯示，台灣綠蜂膠及巴西蜂膠對P. gingivalis、A. actinomycetemcomitans沒有抗菌效果，但台灣綠蜂膠對S. mutans有抗菌能力甚至優於巴西蜂膠，且個別蜂膠素(Propolins)與蜂膠素組合針對S. mutans都顯示良好的抗菌能力。而台灣綠蜂膠針對骨母細胞的礦化效果顯然不佳，但有抗齲齒細菌貼附的活性能力。此外，台灣綠蜂膠與蜂膠素都有促進對脂肪前身細胞分化為脂肪細胞的活性。 在本研究裡面，雖無法有效的治療牙周病菌與刺激骨頭礦化，但對齲齒與代謝疾病有一定的抑制效果，所以不可忽略台灣綠蜂膠的價值及其多種生物活性，或許在未來台灣綠蜂膠的應用是把台灣綠蜂膠、蜂膠素與蜂膠素組合當每日保健食品來預防疾病的發生，而已患有疾病的患者，除利用現今醫學治療與藥物控制外，亦可搭配台灣綠蜂膠來降低疾病惡化。 | zh_TW |
| dc.description.abstract | With the development of science and technology, the lifestyle and eating habits have changed. It has brought many diseases. At present, dental caries, periodontal disease and chronic metabolic diseases are common diseases in the world. The cost of treating these diseases is enormous. Now, people pay more attention to their health. How to reduce the costs of treating those diseases? How to effectively prevent these diseases coming? There are the goals that the biomedical researchers actively trying to achieve. Propolis has been reported that have considerable biological activities. It has been used as a traditional medical drug in the world. Taiwanese green propolis is not only unique but also have many biological activities, such as anti-microbial, anti-inflammatory, anti-oxidant and anti-tumor, etc. We want to use the considerable biological activities of Taiwanese green propolis and propolins to explore whether it can prevent these diseases or inhibit these diseases.
Exploring the diverse biological activities of Taiwanese green propolis extract. Using minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) experiments to find out the results of antibacterial activity that propolis, propolins and the combination of propolins to resist S. mutans which causes dental caries and P. gingivalis and A. actinomycetemcomitans which causes periodontal diseases. Taiwanese green propolis was used to measure bone mineralization of Human Embryonic Palatal Mesenchyme cells (HEPM). Designing an oral pattern experiments to understand that S. mutans and E. coli attaching to the bovine teeth. Using the microscopic and liquid culture of the bacteria to obtain the antibacterial attaching effect of Taiwanese green propolis. Finally, Taiwanese green propolis was used to stimulate the differentiation of mouse mesenchymal stem cells (C3H) into adipocytes. The experimental results show that Taiwanese green propolis and Brazilian propolis have no antibacterial effect on P. gingivalis and A. actinomycetemcomitans, but Taiwanese green propolis has good antibacterial activity to S. mutans, more than Brazilian propolis. The individual propolins and the combination of propolins show good antibacterial ability to S. mutans. The mineralization effect of Taiwanese green propolis to HEPM is obviously not good. But Taiwanese green propolis has good activity ability for bacteria to avoid adhering to dental. In addition, Taiwanese green propolis and propolins can promote C3H cells differentiate into adipocytes. In this study, even though it is not effective in the treatment of periodontal disease’s bacterial and the promotion of bone hyperplasia, it has a certain inhibitory effect on dental caries and metabolic diseases. Therefore, the value of Taiwanese green propolis and its diverse biological activities cannot be ignored. Perhaps the application of Taiwanese green propolis, propolins and the combination of propolins is as a daily health food to prevent the occurrence of diseases in the future. The patients who already have diseases, in addition to using current medical treatment and drug control, being treated with Taiwanese green propolis to reduce disease progression. | en |
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| dc.description.tableofcontents | 誌謝 I
中文摘要 II ABSTRACT IV 目錄 VI 圖目錄 IX 表目錄 XII CHAPTER 1 緒論 1 1.1 前言 1 CHAPTER 2 文獻回顧 2 2.1 生物膜(Biofilm) 2 2.1.1 生物膜的定義 2 2.1.2 生物膜的生成 2 2.2 口腔疾病與致病菌 4 2.2.1 口腔微生物 4 2.2.2 齲齒(Dental Caries) 5 2.2.3 牙周病(Periodontal disease) 6 2.3 肥胖與糖尿病 7 2.3.1 肥胖 7 2.3.2 糖尿病 8 2.3.3 糖尿病之發病機制 8 2.3.4 葡萄糖恆定(Glucose homeostasis) 9 2.3.5 脂肪細胞的分化與代謝 11 2.4 相互影響 12 2.4.1 肥胖與糖尿病和口腔疾病之關聯 12 2.5 蜂膠 13 2.5.1 蜂膠 13 2.5.2 台灣綠蜂膠 14 2.5.3 台灣綠蜂膠之蜂膠素 15 CHAPTER 3 研究動機與目的 17 3.1 研究動機 17 3.2 研究目的 17 CHAPTER 4 實驗材料、儀器與方法 18 4.1 實驗流程圖 18 4.2 實驗材料 19 4.2.1 萃取及分析蜂膠及蜂膠素配製部分 19 4.2.2 細菌培養部分 20 4.2.3 細胞株培養部分 22 4.2.4 細菌貼附的部分 26 4.2.5 實驗耗材 27 4.3 實驗儀器 28 4.4 實驗方法 30 萃取蜂膠及蜂膠素製備實驗方法部分 30 4.4.1 細菌實驗方法部分 31 4.4.2 細胞株實驗方法部分 34 4.4.3 細菌貼附 42 4.4.4 統計方法 46 CHAPTER 5 實驗結果 48 5.1 台灣綠蜂膠萃取分析與蜂膠素收集 48 5.1.1 利用HPLC分析台灣綠蜂膠 48 5.1.2 利用HPLC分析收集到之蜂膠素純度 49 5.1.3 不同蜂膠素的含量 53 5.2 蜂膠萃取與蜂膠素抗微生物活性 54 5.2.1 台灣綠蜂膠、巴西蜂膠與漱口液抗微生物活性 54 5.2.2 台灣綠蜂膠之蜂膠素抗微生物活性 54 5.2.3 蜂膠素組合協同作用抗微生物活性 55 5.3 台灣綠蜂膠萃取物對人類胚胎上顎間質細胞之實驗 57 5.3.1 台灣綠蜂膠萃取物對人類胚胎上顎間質細胞(HEPM)之毒性分析(MTT assay) 57 5.3.2 台灣綠蜂膠萃取物對人類胚胎上顎間質細胞(HEPM)之鹼性磷酸酶試驗(ALP assay) 58 5.4 抗菌貼附 59 5.4.1 顯微鏡觀察牙齒表面貼附菌之螢光染色 59 5.4.2 台灣綠蜂膠萃取物抗菌貼附之菌落數結果 60 5.4.3 不同濃度之台灣綠蜂膠對牛牙之色差 66 5.4.4 抗菌貼附之牙齒表面蜂膠分析 67 5.5 台灣綠蜂膠之蜂膠素對小鼠間質幹細胞(C3H)之實驗 70 5.5.1 台灣綠蜂膠之蜂膠素對小鼠間質幹細胞(C3H)之毒性分析(MTS assay) 70 5.5.2 台灣綠蜂膠之蜂膠素對小鼠間質幹細胞(C3H)之脂肪分化結果 73 CHAPTER 6 討論 76 6.1 蜂膠萃取物與蜂膠素抗微生物活性 76 6.1.1 蜂膠萃取物的抗微生物活性 76 6.1.2 台灣綠蜂膠之蜂膠素與蜂膠素組合之抗微生物活性 77 6.2 台灣綠蜂膠萃取物對人類胚胎上顎間質細胞之實驗 78 6.2.1 台灣綠蜂膠萃取物對人類胚胎上顎間質細胞(HEPM)之毒性分析(MTT assay)與鹼性磷酸酶試驗(ALP assay) 78 6.3 抗菌貼附 78 6.3.1 顯微鏡觀察牙齒表面貼附菌之螢光染色 78 6.3.2 台灣綠蜂膠萃取物抗菌貼附之菌落數結果 78 6.3.3 不同濃度之台灣綠蜂膠對牛牙之色差 79 6.3.4 抗菌貼附之牙齒表面蜂膠官能基分析 79 6.4 台灣綠蜂膠之蜂膠素對小鼠間質幹細胞(C3H)之實驗 79 6.4.1 台灣綠蜂膠之蜂膠素對小鼠間質幹細胞(C3H)之毒性分析(MTS assay) 79 6.4.2 台灣綠蜂膠之蜂膠素對小鼠間質幹細胞(C3H)之脂肪分化結果 80 CHAPTER 7 結論 81 CHAPTER 8 參考文獻 82 | |
| dc.language.iso | zh-TW | |
| dc.title | 台灣綠蜂膠萃取物對齲齒、牙周病及脂肪細胞分化之影響 | zh_TW |
| dc.title | Effects of Taiwanese Green Propolis extract on dental caries, periodontal disease, and adipocyte differentiation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳裕文,游玉祥 | |
| dc.subject.keyword | 齲齒,牙周病,肥胖,糖尿病,台灣綠蜂膠,蜂膠素, | zh_TW |
| dc.subject.keyword | Dental caries,Periodontal disease,Obesity,Diabetes,Taiwanese green propolis,Propolins, | en |
| dc.relation.page | 90 | |
| dc.identifier.doi | 10.6342/NTU202000336 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2020-02-05 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
| 顯示於系所單位： | 口腔生物科學研究所 | |
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