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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳岳隆(Yueh-Lung Wu) | |
| dc.contributor.author | Yi-Chi Chuang | en |
| dc.contributor.author | 莊依奇 | zh_TW |
| dc.date.accessioned | 2021-06-07T17:28:36Z | - |
| dc.date.copyright | 2021-02-22 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-02-02 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15216 | - |
| dc.description.abstract | 桿狀病毒為為雙股環狀DNA病毒，在自然界中可以感染多種節肢動物，而最主要的寄主則通常為鱗翅目幼蟲。加州苜蓿夜蛾核多角體病毒 (AcMNPV) 為目前研究最廣泛的桿狀病毒，並且可以感染多達39種的昆蟲，而家蠶核核多角體病毒 (BmNPV) 是一種對於家蠶有高度宿主專一性的桿狀病毒。這兩種桿狀病毒在序列及基因結構性具有高度相似性，但在寄主的範圍卻沒有任何重疊，BmNPV 只會感染家蠶 (Bombyx mori)，而AcMNPV的允許寄主 (permissive host) -斜紋夜蛾 (Spodoptera litura) 則無法被BmNPV感染，這種寄主專一性的現象非常特別，前人研究著重於研究病毒基因對於寄主專一性所造成的影響，但寄主專一性可以被許多不同因素影響，本研究則從寄主生理代謝的變化方向去切入。在本研究所得到的結果指出，在病毒感染了非允許寄主 (non-permossive host) 後，寄主體內血淋巴中的海藻糖含量減少，而接著在海藻糖酶及糖原磷解酶的表現量上升代表了酵素的作用使得寄主體內儲存糖被分解成細胞可利用之葡萄糖，而也接連影響了脂肪體中葡萄糖的含量及糖解作用的改變，因病毒並不能在非允許寄主體內複製，我們推測這些能量上的改變可能是為了提供給昆蟲的免疫系統，實驗結果也證明了在感染病毒後，非允許寄主體內細胞性免疫的吞噬作用增加，參與體液性免疫的基因表現量也上升，非允許寄主體內體液性免疫最下游之抗菌肽的表現量也因為病毒的感染而增加，免疫系統的啟動可能是造成病毒無法在非允許寄主體內複製的原因之一，進而利用 2DG 抑制寄主的醣類代謝使寄主無法產生能量後，病毒感染非寄主的能力上升，也證明病毒的寄主專一性與寄主體內醣類代謝有一定的關聯。本研究利用探討病毒感染後寄主的生理代謝變化，證明了寄主自身生理代謝可能是影響病毒感染專一性的原因之一。 | zh_TW |
| dc.description.abstract | Baculoviruses are double-stranded circular DNA viruses with genomes of approximately 80-180 kb. They can infect many arthropod species, with lepidoptera larvae being the most common host. Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), which can infect 39 species of lepidoptera, is the most studied baculovirus to date. Bombyx mori nucleopolyhedrovirus (BmNPV) is a major silkworm pathogen which has developed high host specificity to Bombyx mori. These two viruses are very similar in genetic structure and sequence, but have no overlapping host range. BmNPV can only infect Bombyx mori, but Bombyx mori cannot be infected by AcMNPV. Similarly, Spodoptera litura, the permissive host of AcMNPV, cannot infected by BmNPV. Many previous studies have discussed the conditions which affect the host specificity of these two viruses. Our study found that when AcMNPV and BmNPV infect their non-permissive hosts, the blood sugar composition of the host will be changes, thus causing changes in carbohydrate metabolism. We believe that these changes in energy flow may induce the immune response of non-permissive hosts. Our research has confirmed that after infection with non-permissive viruses, both cellular and humoral immunity are enhanced. Humoral response involves the production of various antimicrobial peptides (AMPs). Our results show that gloverin, a kind of antimicrobial peptides, may be an important factor that influences baculovirus infection. The activation of the immune system may be one of the reasons why the virus cannot replicate in non-permissive hosts. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T17:28:36Z (GMT). No. of bitstreams: 1 U0001-0202202113250800.pdf: 2477312 bytes, checksum: bc12b9678a70a443cdf2a5560a1e7a7e (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 中文摘要 i Abstract ii 目錄 iii 圖次 v 壹、 前言 1 貳、 往昔研究 4 2.1 桿狀病毒 (Baculovirus) 4 2.2 加州苜蓿蛾核多角體病毒 (AcMNPV) 及家蠶核多角體病毒 (BmNPV) 4 2.3 影響桿狀病毒產生寄主專一性的因素 5 2.4 昆蟲體內能量代謝：醣類代謝 5 2.5 昆蟲免疫 6 參、 材料與方法 9 3.1 斜紋夜蛾與家蠶飼養 9 3.2 細胞與病毒純化 9 3.3 蟲體注射 9 3.4 分離斜紋夜蛾及家蠶血淋巴 (hemolymph) 和脂肪體 (fat body) 10 3.5 海藻糖 (trehalose) 含量測定 10 3.6 糖原 (glycogen) 含量測定 10 3.7 葡萄糖 (glucose) 含量測定 11 3.8 核酸萃取 11 3.9 即時定量聚合酶連鎖反應 12 3.10 病毒核酸萃取 12 3.11 吞噬作用測定 (phagocytosis assay) 13 3.12 統計分析 13 肆、 結果 15 4.1 病毒感染非允許寄主後之醣類代謝變化 15 4.1.1 海藻糖含量變化 (Trehalose assay) 15 4.1.2 脂肪體中糖原 (glycogen) 濃度變化 16 4.1.3 昆蟲血淋巴及脂肪體中儲存糖的催化酵素變化 16 4.1.4 葡萄糖含量變化 (Glucose Assay) 17 4.1.5 糖解作用的影響 18 4.2 寄主感染非允許病毒後之免疫反應之變化 19 4.2.1 細胞性免疫 19 4.2.2 感染病毒後免疫途徑之基因表現量變化 20 4.2.3 病毒感染後寄主體內抗菌肽 gloverin 表現量變化 22 4.3 以2DG處理SL1A細胞株及BmN細胞株後病毒滴度 (titer) 的變化 23 伍、 討論 25 陸、 參考文獻 50 柒、 附錄 58 | |
| dc.language.iso | zh-TW | |
| dc.subject | 醣類代謝 | zh_TW |
| dc.subject | 桿狀病毒 | zh_TW |
| dc.subject | 寄主專一性 | zh_TW |
| dc.subject | Baculoviruses | en |
| dc.subject | carbohydrate metabolism | en |
| dc.subject | host specificity | en |
| dc.title | 醣類代謝差異影響桿狀病毒感染專一性之研究 | zh_TW |
| dc.title | Study the effect of carbohydrate metabolism on the specificity of baculovirus infection | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 109-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃榮南(Rong-Nan Huang), 李松泰(Song-Tay Lee),吳宗遠(Tzong-Yuan Wu),乃育昕(Yu-Shin Nai) | |
| dc.subject.keyword | 桿狀病毒,寄主專一性,醣類代謝, | zh_TW |
| dc.subject.keyword | Baculoviruses,host specificity,carbohydrate metabolism, | en |
| dc.relation.page | 63 | |
| dc.identifier.doi | 10.6342/NTU202100371 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2021-02-03 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 昆蟲學研究所 | zh_TW |
| 顯示於系所單位： | 昆蟲學系 | |
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