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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 林萬寅(Wann-Yin Lin) | |
dc.contributor.author | Sin-Kai Yu | en |
dc.contributor.author | 游信凱 | zh_TW |
dc.date.accessioned | 2021-05-20T21:49:55Z | - |
dc.date.available | 2012-08-19 | |
dc.date.available | 2021-05-20T21:49:55Z | - |
dc.date.copyright | 2010-08-19 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-31 | |
dc.identifier.citation | 1. Greer, L F & Szalay A A Luminescence 2002, 17, 43
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10685 | - |
dc.description.abstract | Chemiluminescence (CL) is produced by the exothermic reaction. This study focused on the dual chemiluminescence of luminol-Co(II)-NH2OH. We found that concentrations of Co(II), EDTA, NH2OH and pH could lead to dual chemiluminescence. To know more about it, we’ve tried many conditions to construct the luminol-Co(II)-NH2OH system.
The results from stopped flow experiments showed that low concentrations of Co(II)(less than 3 uM) produced only one peak. At higher concentrations of Co(II) dual chemiluminescence appeared. Addtion of EDTA caused suppression and delayed of dual CL, suggesting the importance of free Co(II). Moreover, concentration of NH2OH and pH values between 12.5 and 13.5 were also required for the observation of dual CL. In order to know the mechanism of the dual chemiluminescence of luminol-Co(II)-NH2OH system, we tested a series of free radical scanvengers for their effect on CL emission. In the present of 1O2 scanvengers like NaN3, 1,4-diazabicyclo[2.2.2]octane and DMF, dual CL emission was enhanced. Addition of ˙OH scanvengers such as CH3OH , DMSO and uric acid, inhibited peak 2 of dual CL. Captopril, a ˙O2- scanvenger, could inhibit and delay CL peaks. We concluded that ˙O2- is involved in the CL emission of peak 1, while ˙O2- and ˙OH play key role for the second CL peak. By using the dual chemiluminescence of luminol-Co(II)-NH2OH system, we tested 58 compounds including amino acids, vitamins, antioxidants, reducing compounds and drugs for their effect on the CL emission. We found that phenolic compounds showed inhibiting effect on our CL system, especially benzenediol compounds, flavonoids and polyphenolic compounds. It was found that compounds bearing a dihydroxybenzene group, exhibited best inhibition on peak 1. Previous studies have suggested that dihydroxybenzenes were good superoxide anion radical scanvenger, causing CL inhibition. We proposed that superoxide anion radical plays a critical role in both CL peaks. The results suggest that this system could be used to screen the scanvenge activities and antioxidant activities of the compounds. Using the selectivity from inhibitng either peak 1 or peak 2, we could make use of the dual chemiluminescence of luminol-Co(II)-NH2OH for the analytic detection. | en |
dc.description.provenance | Made available in DSpace on 2021-05-20T21:49:55Z (GMT). No. of bitstreams: 1 ntu-99-R95223082-1.pdf: 1923686 bytes, checksum: c15825fc9a7aae77a5e8ea13177da730 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 中文摘要..................... I
英文摘要.................... III 目錄...................... V 附圖目錄.................... VII 表目錄.................... IX 第一章 緒論.................. 1 1-1 化學發光簡介.................. 1 1-2 化學發光原理................. 3 1-3 Luminol 化學發光系統簡介.............. 5 1-4 Luminol與Co(II)對化學發光的影響........... 7 1-5 活性含氧物質對化學發光的影響............ 9 1-6 還原劑對化學發光的影響.............. 11 1-7 研究動機.................. 12 第二章 實驗材料與儀器介紹.............. 13 2-1 溶液配製.................. 13 2-2 實驗裝置.................. 14 2-3 停止流光譜儀原理與架設.............. 15 2-3-1 停止流光譜儀原理................ 15 2-4 實驗藥品.................. 19 第三章 結果與討論.......... 22 3-1 Luminol-Co(II)-NH2OH 雙重化學發光系統.......... 22 3-1-1 Luminol-Co(II)-NH2OH 系統測試........... 22 3-1-2-1 Co(II) 濃度對雙重化學發光的影響........... 24 3-1-2-2 EDTA對雙重化學發光的影響 ............ 27 3-1-3 Luminol 濃度對雙重化學發光的影響.......... 30 3-1-4 NH2OH 濃度對雙重化學發光的影響........... 32 3-1-5 pH 值對雙重化學發光的影響............. 35 3-2 自由基消滅劑對雙重化學發光的影響.......... 37 3-2-1 超氧自由基消滅劑測試............... 38 3-2-2 氫氧自由基消滅劑測試............... 43 3-2-3 單態氧消滅劑測試............... 49 3-3 生化有機分子測試................ 56 3-3-1 初步篩選................... 56 3-3-2 八種分子深入測試............... 61 3-3-2-1 thymol測試................. 61 3-3-2-2 tannic acid測試................ 65 3-3-2-3 o-phenylenediamine測試.............. 68 3-3-2-4 normetanephrine測試............... 71 3-3-2-5 chlorogenic acid測試............... 75 3-3-2-6 catechin測試................ 78 3-3-2-7 3,4-dihydroxybenzoic acid測試........... 81 3-3-2-8 octopamine測試............... 85 3-3-3 Luminol-Co(II)-NH2OH系統發光機制之探討......... 88 第四章 結論................... 90 第五章 參考文獻................. 91 | |
dc.language.iso | zh-TW | |
dc.title | Luminol-Co(II)-NH2OH系統之雙重化學發光探討與其在分析上的應用 | zh_TW |
dc.title | Dual Chemiluminescence of luminol-Co(II)-NH2OH System and its Applications | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉春櫻(Chuen-Ying Liu),何國榮(Guor-Rong Her) | |
dc.subject.keyword | 化學發光,自由基消滅劑,停止流儀,酚類化合物,鈷離子, | zh_TW |
dc.subject.keyword | luminol,chemiluminescence,Co(II),free radical scanvenger,phenolic compounds,stopped flow spectrometry, | en |
dc.relation.page | 92 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2010-08-02 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 化學研究所 | zh_TW |
顯示於系所單位: | 化學系 |
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