請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10614完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 曹幸之(Shing-Jy Tsao) | |
| dc.contributor.author | Yi-Cheng Chou | en |
| dc.contributor.author | 周奕成 | zh_TW |
| dc.date.accessioned | 2021-05-20T21:43:52Z | - |
| dc.date.available | 2010-08-12 | |
| dc.date.available | 2021-05-20T21:43:52Z | - |
| dc.date.copyright | 2010-08-12 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-10 | |
| dc.identifier.citation | 台灣地區現有作物栽培品種名錄十字花科篇。1994。行政院農業委
員會台灣省農業試驗所特刊43號。 洪立、黃涵、嚴新富。1992。蔬菜名彙集。國立台灣大學園藝系。 臺北。 孫德嶺、趙前程、宋文芹、陳瑞陽。2002。花椰菜類蔬菜自交系基 因組間親緣關係的AFLP分析。園藝學報。29(1):72-74。 陳怡婷。2004。以簡單重複序列分子標誌探討花椰菜品種及白菜類 的遺傳變異。國立台灣大學園藝學研究所碩士論文。 張連宗。1998。台灣十字花科蔬菜品種改良。十字花科蔬菜產業發 展研討會專刊。 農業委員會。2005。台灣農家要覽-農作篇(二)。豐年社。台北市。 劉海濤、關佩聰。1998。芥藍的分類學研究。華南農業大學學報 19:82-86。 譚冠寧、藍永慶、李麗淑、趙坤、何海旺、蔣菁。2007。青花筍 (西蘭薹) 新品種桑甜2號。中國蔬菜10:63~64。 Anderson, J.A., G.A. Churchill, J.E. Autrique, S.D. Tanksley, and M.E. Sorrells. 1993. Optimizing parental selection for genetic-linkage maps. Genome 36:181-186. Ayele, M., B.J. Haas, N. Kumar, H. Wu, Y.L. Xiao, S. Van Aken, T.R. Utterback, J.R. Wortman, O.R. White, and C.D. Town. 2005. Whole genome shotgun sequencing of Brassica oleracea and its application to gene discovery and annotation in Arabidopsis. Genome Res 15:487-495. Babula, D., M. kaczmarek, P.A. Ziolkowski, and J. Sadowski. 2007. Brassica oleracea, p. 227-285. In: C. Kole.(ed). Genome Mapping and Molecular Breeding in Plants - vegetable. Vol. Volume 5.ed. Springer Berlin Heidelberg. New York. Blair, M., H.F. Buendia, M. Giraldo, I. Metais, and D. Peltier. 2008. Characterization of AT-rich microsatellites in common bean (Phaseolus vulgaris L.). Theor. Appl. Genet. 118:91-103. Bonhomme, S., F. Budar, M. Ferault, and G. Pelletier. 1991. A 2.5 kb NcoI fragment of Ogura radish mitochondria DNA is correlated with cytoplasmic male sterility in Brassica cybrids. Curr. Genet. 19:121-127. Bonhomme, S., F. Budar, D. Lancelin, I. Small, M.C. Defrance, and G. Pelletier. 1992. Sequence and transcript analysis of the Nco2.5 Ogura specific fragment correlated with cytoplasmic male sterility in Brassica cybrids. Mol. Gen. Genet. 235:340-348. Botstein, D., R.L. White, M. Skolnick, and R.W. Davis. 1980. Construction of a genetic-linkage map in man usingrestriction fragment length polymorphisms. Am. J. Hum. Genet. 32:314-331. Bouhadida, M., A.M. Casas, M.J. Gonzalo, P. Arus, M.A. Moreno, and Y. Gogorcena. 2009. Molecular characterization and genetic diversity of Prunus rootstocks. Sci. Hortic. 120:237-245. Boutin-Ganache, I., M. Raposo, M. Raymond, and C.F. Deschepper. 2001. M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. BioTechniques 31:24-+. Bredemeijer, G.M.M., R.J. Cooke, M.W. Ganal, R. Peeters, P. Isaac, Y. Noordijk, S. Rendell, J. Jackson, M.S. Roder, K. Wendehake, M. Dijcks, M. Amelaine, V. Wickaert, L. Bertrand, and B. Vosman. 2002. Construction and testing of a microsatellite database containing more than 500 tomato varieties. Theor. Appl. Genet. 105:1019-1026. Burgess, B., H. Mountford, C.J. Hopkins, C. Love, A.E. Ling, G.C. Spangenberg, D. Edwards, and J. Batley. 2006. Identification and characterization of simple sequence repeat (SSR) markers derived in silico from Brassica oleracea genome shotgun sequences. Mol. Ecol. Notes 6:1191-1194. Camargo, L.E.A., L. Savides, G. Jung, J. Nienhuis, and T.C. Osborn. 1997. Location of the self- incompatibility locus in an RFLP and RAPD map of Brassica oleracea. J. Hered. 88:57-60. Coelho, R.C., M.A. Faria, J. Rocha, A. Reis, M. Oliveira, and E. Nunes. 2009. Assessing genetic variability in germplasm of Phaseolus vulgaris L. collected in Northern Portugal. Sci. Hortic. 122:333-338. Crisp, P. 1982. The use of an evolutionary scheme for cauliflowers in the screening of gemetic resources. Euphytica 31:725-734. Dixon, G.R. 2007. Vegetable brassicas and related crucifers.ed. CABI Pub., Cambridge, MA. Doyle, J.J. and J.L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15. Figdore, S.S., M.E. Ferreira, M.K. Slocum, and P.H. Williams. 1993. Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69:33-44. Fukino, N., Y. Yoshioka, N. Kubo, M. Hirai, M. Sugiyama, Y. Sakata, and S. Matsumoto. 2008. Development of 101 novel SSR markers and construction of an SSR-based genetic linkage map in cucumber (Cucumis sativus L.). Breed. Sci. 58:475-483. Gao, M.Q., G.Y. Li, B. Yang, D. Qiu, M. Farnham, and C. Quiros. 2007. High-density Brassica oleracea linkage map: identification of useful new linkages. Theor. Appl. Genet. 115:277-287. Ghislain, M., J. Nunez, M. del Rosario Herrera, J. Pignataro, F. Guzman, M. Bonierbale, and D.M. Spooner. 2009. Robust and highly informative microsatellite- based genetic identity kit for potato. Mol. Breed. 23:377-388. Gray, A.R. 1982. Taxonomy and evolution of broccoli (Brassica oleracea var. italica). Econ. Bot. 36:397- 410. Gupta, S.K. 2009. Biology and breeding of crucifers.ed. CRC Press, Boca Raton. Hale, A. and M.W. Farnham. 2005. A comparative study of SRAP, AFLP, and SSR markers for detecting genetic differences among elite broccoli inbreds. HortScience 40:998-999. Hancock, J.F. 2004. Plant evolution and the origin of crop species.ed. CABI Pub., New York, NY. Hayashi, K., K. Shimazu, H. Yaegaki, M. Yamaguchi, H. Iketani, and T. Yamamoto. 2008. Genetic diversity in fruiting and flower-ornamental Japanese apricot (Prunus mume) germplasms assessed by SSR markers. Breed. Sci. 58:401-410. Hayden, M.J., T.M. Nguyen, A. Waterman, and K.J. Chalmers. 2008a. Multiplex-ready PCR: A new method for multiplexed SSR and SNP genotyping. BMC Genomics 9:80. Hayden, M.J., T.M. Nguyen, A. Waterman, G.L. McMichael, and K.J. Chalmers. 2008b. Application of multiplex- ready PCR for fluorescence-based SSR genotyping in barley and wheat. Mol. Breed. 21:271-281. Howell, E.C., G.C. Barker, G.H. Jones, M.J. Kearsey, G.J. King, E.P. Kop, C.D. Ryder, G.R. Teakle, J.G. Vicente, and S.J. Armstrong. 2002. Integration of the cytogenetic and genetic linkage maps of Brassica oleracea. Genetics 161:1225-1234. Iniguez-Luy, F.L., L. Lukens, M.W. Farnham, R.M. Amasino, and T.C. Osborn. 2009. Development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling Brassica rapa and B. oleracea. Theor. Appl. Genet. 120:31-43. Iniguez-Luy, F.L., A.V. Voort, and T.C. Osborn. 2008. Development of a set of public SSR markers derived from genomic sequence of a rapid cycling Brassica oleracea L. genotype. Theor. Appl. Genet. 117:977- 985. Jahangir, M., H.K. Kim, Y.H. Choi, and R. Verpoorte. 2009. Health-Affecting Compounds in Brassicaceae. Comprehensive Rev. Food Sci. Food Safety 8:31-43. Karagyozov, L., I.D. Kalcheva, and V.M. Chapman. 1993. Construction of random small insert genomic libraries highly enriched for simple sequence repeats. Nucleic Acids Res. 21:3911-3912. Kays, S.J. and J.C.S. Dias. 1995. Common names of commercially cultivated vegetables of the world in 15 languages. Econ. Bot. 49:115-152. Kearsey, M.J., L.D. Ramsay, D.E. Jennings, D.J. Lydiate, E.J.R. Bohuon, and D.F. Marshall. 1996. Higher recombination frequencies in female compared to male meisoses in Brassica oleracea. Theor. Appl. Genet. 92:363-367. Kennard, W.C., M.K. Slocum, S.S. Figdore, and T.C. Osborn. 1994. Genetic analysis of morphological variation in Brassica oleracea using molecular markers. Theor. appl. genet. 87:721-732. Khattak, J.Z.K., J.L. Christiansen, A.M. Torp, and S.B. Andersen. 2007. Genic microsatellite markers for discrimination of spinach cultivars. Plant Breed. 126:454-456. Kianian, S.F. and C.F. Quiros. 1992. Generation of a Brassica oleracea composite RFLP map - linkage arrangements among various populations and evolutionary implications. Theor. Appl. Genet.84:544- 554. Kimura, T., M. Yagi, C. Nishitani, T. Onozaki, Y. Ban, and T. Yamamoto. 2009. Development of SSR Markers in Carnation (Dianthus caryophyllus). J. Jpn. Soc.Hortic. Sci. 78:115-123. Kong, Q., C. Xiang, Z. Yu, C. Zhang, F. Liu, C. Peng, and X. Peng. 2007. Mining and charactering microsatellites in Cucumis melo expressed sequence tags from sequence database. Mol. Ecol. Notes 7:281-283. Kresovich, S., A.K. Szewcmcfadden, S.M. Bliek, and J.R. McFerson. 1995. Abundance and characterization of simple-sequence repeat (SSRs) Isolated from a size- fractionated genomic library of Brassica napus (rapeseed). Theor. Appl. Genet. 91:206-211. Krishnasamy, S. and C.A. Makaroff. 1993. Characterization of the radish mitochondrial orf B locus - possible relationship with male sterility in Ogura radish. Curr. Genet. 24:156-163. Kushad, M.M., A.F. Brown, A.C. Kurilich, J.A. Juvik, B.P. Klein, M.A. Wallig, and E.H. Jeffery. 1999. Variation of glucosinolates in vegetable crops of Brassica oleracea. J. Agric. Food Chem. 47:1541- 1548. Lacis, G., I. Rashal, S. Ruisa, V. Trajkovski, and A.F. Iezzoni. 2009. Assessment of genetic diversity of Latvian and Swedish sweet cherry (Prunus avium L.) genetic resources collections by using SSR (microsatellite) markers. Sci. Hortic. 121:451-457. Lagercrantz, U., H. Ellegren, and L. Andersson. 1993. The abundance of various polymorphic microdatellite motifs differs between plants and vertebrates. Nucleic Acids Res. 21:1111-1115. Lai, Y.L. and F.Z. Sun. 2003. The relationship between microsatellite slippage mutation rate and the number of repeat units. Mol. Biol. Evol. 20:2123- 2131. Landry, B.S., N. Hubert, R. Crete, M.S. Chang, S.E. Lincoln, and T. Etoh. 1992. A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race-2 of plasmodiophora Brassicae. Genome 35:409-420. Lazaro, A. and I. Aguinagalde. 1998a. Genetic diversity in Brassica oleracea L. (Cruciferae) and wild relatives (2n = 18) using isozymes. Ann. Bot. 82:821-828. Lazaro, A. and I. Aguinagalde. 1998b. Genetic diversity in Brassica oleracea L. (Cruciferae) and wild relatives (2n = 18) using RAPD markers. Ann. Bot. 82:829-833. Leroy, X.J., K. Leon, and M. Branchard. 2000. Characterisation of Brassica oleracea L. by microsatellite primers. Plant Syst. Evol. 225:235- 240. Li, G., M. Gao, B. Yang, and C.F. Quiros. 2003. Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping. Theor. Appl. Genet. 107:168-180. Li, M.F., X.Q. Zheng, Y.Q. Zhu, X.S. Wang, S.Y. Liang, L. Li, and X.R. Wu. 2006. Development and characterization of SSR markers in lychee (Litchi chinensis). Mol. Ecol. Notes 6:1205-1207. Louarn, S., A.M. Torp, I.B. Holme, S.B. Andersen, and B.D. Jensen. 2007. Database derived microsatellite markers (SSRs) for cultivar differentiation in Brassica oleracea. Genet. Resour. Crop Evol. 54:1717-1725. Lowe, A.J., A.E. Jones, A.F. Raybould, M. Trick, C.L. Moule, and K.J. Edwards. 2002. Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle. Mol. Ecol. Notes 2:7-11. Lowe, A.J., C. Moule, M. Trick, and K.J. Edwards. 2004. Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species. Theor. Appl. Genet. 108:1103-1112. Lu, X.J., L.W. Liu, Y.Q. Gong, L.P. Zhao, X.Y. Song, and X.W. Zhu. 2009. Cultivar identification and genetic diversity analysis of broccoli and its related species with RAPD and ISSR markers. Sci. Hortic. 122:645-648. Ma, K.H., J.G. Kwag, W.G. Zhao, A. Dixit, G.A. Lee, H.H. Kim, I.M. Chung, N.S. Kim, J.S. Lee, J.J. Ji, T.S. Kim, and Y.J. Park. 2009. Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic (Allium sativum L.). Sci. Hortic. 122:355-361. Mansfield, D.C., A.F. Brown, D.K. Green, A.D. Carothers, S.W. Morris, H.J. Evans, and A.F. Wright. 1994. Automation of genetic linkage analysis using fluorescent microsatellite markers. Genomics 24:225-233. Metais, I., B. Hamon, R. Jalouzot, and D. Peltier. 2002. Structure and level of genetic diversity in various bean types evidenced with microsatellite markers isolated from a genomic enriched library. Theor. Appl. Genet. 104:1346-1352. Miller, M.P. 1997. Tools for population genetic analysis (TFPGA) 1.3:A Windows program for the analysis of allozyme and molecular population genetic data. http://www.marksgeneticsoftware.net/tfpga.htm (July 10, 2010) Morales, M., V. Decker, and L. Ornella. 2010. Analysis of genetic diversity in Argentinian heterotic maize populations using molecular markers. Cien. Inv. Agr. 37:151-160. Moriguchi, K., C. Kimizuka-Takagi, K. Ishii, and K. Nomura. 1999. A genetic map based on RAPD, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea. Breed. Sci. 49:257-265. Nguyen, H.T. and X. Wu. 2005. Molecular marker systems for genetic mapping, p. 23-52. In: K. Meksem and G. Kahl (eds.). The handbook of plant genome mapping.ed. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Ogura, H. 1968. Studies on the new male-sterility in japanese radish, with special reference to the utilization of this sterility towards the practical rasising of hybrid seeds. Memoirs of the Faculty of Agriculture, Kagoshima University 6:39-78. Ostrander, E.A., P.M. Jong, J. Rine, and G. Duyk. 1992. Construction of small - insert genomic DNA libraries highly enriched for microsatellite repeat sequences. P Natl Acad Sci USA 89:3419-3423. Phippen, W.B., S. Kresovich, F.G. Candelas, and J.R. McFerson. 1997. Molecular characterization can quantify and partition variation among genebank holdings: A case study with phenotypically similar accessions of Brassica oleracea var capitata L (cabbage) 'Golden Acre'. Theor. Appl. Genet. 94:227-234. Portis, E., I. Nagy, Z. Sasvari, A. Stagel, L. Barchi, and S. Lanteri. 2007. The design of Capsicum spp. SSR assays via analysis of in silico DNA sequence, and their potential utility for genetic mapping. Plant Sci. 172:640-648. Reif, J.C., A.E. Melchinger, X.C. Xia, M.L. Warburton, D.A. Hoisington, S.K. Vasal, G. Srinivasan, M. Bohn, and M. Frisch. 2003. Genetic distance based on simple sequence repeats and heterosis in tropical maize populations. Crop Sci 43:1275-1282. Rodriguez-Plaza, P., R. Gonzalez, M.V. Moreno-Arribas, M.C. Polo, G. Bravo, J.M. Martinez-Zapater, M.C. Martinez, and A. Cifuentes. 2006. Combining microsatellite markers and capillary gel electrophoresis with laser-induced fluorescence to identify the grape (Vitis vinifera) variety of musts. Eur. Food Res. Technol. 223:625-631. Rohlf, F.J. 2008. NTSYSpc: Numerical Taxonomy System. Exeter Publishing, Ltd., Setauket, NY. Schlotterer, C. and D. Tautz. 1992. Slippage synthesis of simple sequence DNA. Nucleic Acids Res. 20:211-215. Sebastian, R.L., E.C. Howell, G.J. King, D.F. Marshall, and M.J. Kearsey. 2000. An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations. Theor. Appl. Genet. 100:75-81. Simko, I. 2009. Development of EST-SSR Markers for the Study of Population Structure in Lettuce (Lactuca sativa L.). J. Hered. 100:256-262. Slocum, M.K., S.S. Figdore, W.C. Kennard, J.Y. Suzuki, and T.C. Osborn. 1990. Linkage arrangement of restriction-fragment-length-polymorphism loci in Brassica oleracea. Theor. Appl. Genet. 80:57-64. Smith, L.B. and G.J. King. 2000. The distribution of BoCAL-a alleles in Brassica oleracea is consistent with a genetic model for curd development and domestication of the cauliflower. Mol. Breed. 6:603-613. Song, K.M., T.C. Osborn, and P.H. Williams. 1988a. Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPS) 1. Genome evolution of diploid and amphidiploid species. Theor. Appl. Genet. 75:784-794. Song, K.M., T.C. Osborn, and P.H. Williams. 1988b. Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPS) .2. preliminary analysis of subspecies within Brassica rapa (syn campestris) and Brassica oleracea. Theor. Appl. Genet. 76:593-600. Stagel, A., E. Portis, L. Toppino, G.L. Rotino, and S. Lanteri. 2008. Gene-based microsatellite development for mapping and phylogeny studies in eggplant. BMC genomics 9:357-370. Szewc-McFadden, A.K., S. Kresovich, S.M. Bliek, S.E. Mitchell, and J.R. McFerson. 1996. Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species. Theor. Appl. Genet. 93:534-538. Tantasawat, P., J. Trongchuen, T. Prajongjai, W. Seehalak, and Y. Jittayasothorn. 2010. Variety identification and comparative analysis of genetic diversity in yardlong bean (Vigna unguiculata spp. sesquipedalis) using morphological characters, SSR and ISSR analysis. Sci. Hortic. 124:204-216. Tommasini, L., J. Batley, G.M. Arnold, R.J. Cooke, P. Donini, D. Lee, J.R. Law, C. Lowe, C. Moule, M. Trick, and K.J. Edwards. 2003. The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties. Theor. Appl. Genet. 106:1091-1101. Tonguc, M. and P.D. Griffiths. 2004. Genetic relationships of Brassica vegetables determined using database derived simple sequence repeats. Euphytica 137:193-201. Tsukazaki, H., H. Fukuoka, Y.S. Song, K.I. Yamashita, T. Wako, and A. Kojima. 2006. Considerable heterogeneity in commercial F1 varieties of bunching onion (Allium fistulosum) and proposal of breeding scheme for conferring variety traceability using SSR markers. Breed. Sci. 56:321-326. Tsunoda, S., K. Hinata, and C. Gómez-Campo. 1980. Brassica crops and wild allies : biology and breeding.ed. exclusive distributor ISBS, Forest Grove, Or. U, N. 1935. Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Japan. J. Bot 7:389-452. Vaughan, J.G. 1977. Multidisciplinary study of taxonomy and origin of Brassica crops. Bioscience 27:35-40. Watcharawongpaiboon, N. and J. Chunwongse. 2008. Development and characterization of microsatellite markers from an enriched genomic library of cucumber (Cucumis sativus). Plant Breed. 127:74-81. Westman, A.L. and S. Kresovich. 1998. The potential for cross-taxa simple-sequence repeat (SSR) amplification between Arabidopsis thaliana L. and crop brassicas. Theor. Appl. Genet. 96:272-281. Westman, A.L. and S. Kresovich. 1999. Simple sequence repeat (SSR)-based marker variation in Brassica nigra genebank accessions and weed populations. Euphytica 109:85-92. Xu, P., X.H. Wu, B.G. Wang, Y.H. Liu, D.H. Qin, J.D. Ehlers, T.J. Close, T.T. Hu, Z.F. Lu, and G.J. Li. 2010. Development and polymorphism of Vigna unguiculata ssp unguiculata microsatellite markers used for phylogenetic analysis in asparagus bean (Vigna unguiculata ssp sesquipedialis (L.) Verdc.). Mol. Breed. 25:675-684. Yamagishi, H. and T. Terachi. 1997. Molecular and biological studies on male-sterile cytoplasm in the Cruciferae .4. Ogura-type cytoplasm found in the wild radish, Raphanus raphanistrum. Plant Breed. 116:323-329. Yamagishi, H. and T. Terachi. 2001. Intra- and inter-specific variations in the mitochondrial gene orf138 of Ogura-type male-sterile cytoplasm from Raphanus sativus and Raphanus raphanistrum. Theor. Appl. Genet. 103:725-732. Yang, H., M.W. Sweetingham, W.A. Cowling, and P.M.C. Smith. 2001. DNA fingerprinting based on microsatellite-anchored fragment length polymorphisms, and isolation of sequence-specific PCR markers in lupin (Lupinus angustifolius L.). Mol. Breed. 7:203-209. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10614 | - |
| dc.description.abstract | 本研究利用65個品種，共98份來自7個變種的甘藍類蔬菜材料篩選出26個SSR分子標誌，在所有受試材料中均可擴增出產物。針對此98份材料進行基因座對偶基因分析，目的為建立一套可區分多數甘藍類作物品種的SSR分子標誌，為分子標誌實際應用於甘藍類作物品種鑑定建立基礎，並對各變種間親緣關係進行探討。26個分子標誌共獲得103個對偶基因，各基因座可獲得2至12個對偶基因，平均每個基因座含有3.96個對偶基因。分子標誌可偵測的基因型數量從最少2個至最多27個，平均每個分子標誌可以偵測到6.92個基因型。各基因座PIC值介於0.2635至0.8488，平均為0.5399。以此26個SSR分子標誌可以將本次受試品種均進行區分，各品種之間的遺傳距離介於0.098至0.8605之間，平均的遺傳距離為0.651。主座標分析的結果顯示前三個主成分解釋量分別為14.03 %、10.40 %以及6.78 %，其結果與群集分析結果相同，可將所有材料分為芥藍、青花菜、花椰菜、球莖甘藍、甘藍、抱子甘藍及羽衣甘藍七大群，而兩個青花筍品種則是歸類於青花菜類群中，與預期的七個栽培變種相符合。本研究亦對各變種的可能親緣關係進行探討，由遺傳距離及群集分析結果認為青花菜與花椰菜的親緣關係較近，芥藍與其他變種差異較大，前人研究亦認為芥藍未脫離甘藍類蔬菜的範圍。此外本次試驗在相同品種中發現有差異存在，若欲達成將分子標誌實際應用於品種鑑定的目標，需再進一步掌握品種內存在的變異性。 | zh_TW |
| dc.description.abstract | Twenty six SSR markers are screened by 65 cultivars of Brassica oleracea L. from seven botanical varieties, with 98 plants tested in total. The objectives of this study are to establish a set of SSR markers to distinguish the majority of cole crops (Brassica oleracea), and to serve as a base for including molecular markers as a tool in practical variety identification in cole crops. The genetic relationships between different varieties are also discussed. The 26 primer pairs amplified 103 alleles in total in all plants, the . number of alleles per locus of the selected makers ranged from 2 to 12, with an average of 3.96. Number of genotypes detected by each marker ranged from 2 to 27, with an average of 6.92.The PIC values of different markers range from 0.2635 to 0.8488, with an average of 0.5399.The 26 SSR markers could distinguish all cultivars in this study, the genetic distances between pair-wise cultivars range from 0.098 to 0.8605, with an average of 0.651. Principal coordinate analysis (PCoA) and UPGMA cluster showed similar results, all the 65 cultivars separated into seven groups as the taxonomy of botanical varieties, i.e. Chinese kale, broccoli, cauliflower, kohlrabi, cabbage, Brussels sprouts, and kale. Two Broccolini cultivars were classified to the broccoli group. According to the results of genetic distance and cluster analysis of different varieties, broccoli and cauliflower have the closest phylogenetic relationship, Chinese kale is the most distant variety from the other, however, still in same species. The result of intra-cultivar variations indicates that pre-knowledge of the genetic variability within a cultivar is needed before the application of molecular markers in actual variety identification in cole crops. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T21:43:52Z (GMT). No. of bitstreams: 1 ntu-99-R96628105-1.pdf: 1441865 bytes, checksum: bf769a1d38b4447762f6c4354e25d0c1 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 口試委員會審定…………………………………………………………i
致謝……………………………………………………………………ii 目錄……………………………………………………………………iii 圖表目錄………………………………………………………………iv 摘要………………………………………………………………………v Abstract………………………………………………………………vi 第一章 前言……………………………………………………………1 第二章 前人研究………………………………………………………3 第三章 材料與方法…………………………………………………15 一、植物材料……………………………………………………15 二、植株園藝性狀調查…………………………………………15 三、SSR分子標誌分析…………………………………………18 四、資料分析……………………………………………………20 第四章 結果…………………………………………………………27 一、甘藍類蔬菜品種園藝性狀調查……………………………27 二、參試樣品之對偶基因檢測…………………………………28 三、甘藍類各品種之遺傳歧異度與群集分析…………………36 四、甘藍類各品種之主座標分析………………………………42 五、甘藍類變種間遺傳歧異度與群集分析與主座標分析……42 第五章 討論…………………………………………………………51 結論……………………………………………………………………55 參考文獻………………………………………………………………57 附錄……………………………………………………………………65 | |
| dc.language.iso | zh-TW | |
| dc.title | 建立甘藍類作物品種鑑定之SSR分子標誌 | zh_TW |
| dc.title | Development of SSR markers for variety identification of cole crops (Brassica oleracea L.) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 胡凱康(Kae-Kang Hu) | |
| dc.contributor.oralexamcommittee | 王仕賢,陳榮芳 | |
| dc.subject.keyword | 蕓薹屬,微衛星序列,毛細管電泳,主座標分析,群聚分析, | zh_TW |
| dc.subject.keyword | Brassica,microsatellite,capillary electrophoresis,Principal Coordinate Analysis,UPGMA, | en |
| dc.relation.page | 76 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2010-08-10 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝學研究所 | zh_TW |
| 顯示於系所單位： | 園藝暨景觀學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf | 1.41 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。