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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王淑美 | |
dc.contributor.author | Shih-Hung Yang | en |
dc.contributor.author | 楊士弘 | zh_TW |
dc.date.accessioned | 2021-05-20T21:13:13Z | - |
dc.date.available | 2011-03-03 | |
dc.date.available | 2021-05-20T21:13:13Z | - |
dc.date.copyright | 2011-03-03 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-02-11 | |
dc.identifier.citation | Ahnert-Hilger G, Holtje M, Grosse G, et al. (2004) Differential effects of Rho GTPases on axonal and dendritic development in hippocampal neurones. J Neurochem, 90, 9-18.
Barnes AP, Polleux F (2009) Establishment of axon-dendrite polarity in developing neurons. Annu Rev Neurosci, 32, 347-81. Behl C (2002) Oestrogen as a neuroprotective hormone. Nat Rev Neurosci, 3, 433-42. Behl C, Widmann M, Trapp T, Holsboer F (1995) 17-beta estradiol protects neurons from oxidative stress-induced cell death in vitro. Biochem Biophys Res Commun, 216, 473-82. Benes C, Soltoff SP (2001) Modulation of PKCdelta tyrosine phosphorylation and activity in salivary and PC-12 cells by Src kinases. Am J Physiol Cell Physiol, 280, C1498-510. Bito H, Takemoto-Kimura S (2003) Ca(2+)/CREB/CBP-dependent gene regulation: a shared mechanism critical in long-term synaptic plasticity and neuronal survival. Cell Calcium, 34, 425-430. Bradbury EJ, Khemani S, Von R, King, Priestley JV, McMahon SB (1999) NT-3 promotes growth of lesioned adult rat sensory axons ascending in the dorsal columns of the spinal cord. Eur J Neurosci, 11, 3873-83. Brann DW, Dhandapani K, Wakade C, Mahesh VB, Khan MM (2007) Neurotrophic and neuroprotective actions of estrogen: Basic mechanisms and clinical implications. In Steroids), pp. 381-405. Brodie C, Bogi K, Acs P, et al. (1999) Protein kinase C-epsilon plays a role in neurite outgrowth in response to epidermal growth factor and nerve growth factor in PC12 cells. Cell Growth Differentiation, 10, 183-191. Burguete MC, Torregrosa G, Perez-Asensio FJ, et al. (2006) Dietary phytoestrogens improve stroke outcome after transient focal cerebral ischemia in rats. Eur J Neurosci, 23, 703-10. Chen CH, Chen YJ, Jeng CJ, Yang SH, Tung PY, Wang SM (2007a) Role of PKA in the anti-Thy-1 antibody-induced neurite outgrowth of dorsal root ganglionic neurons. J Cell Biochem, 101, 566-75. Chen CH, Chen YJ, Jeng CJ, Yang SH, Tung PY, Wang SM (2007b) Role of PKA in the anti-Thy-1 antibody-induced neurite outgrowth of dorsal root ganglionic neurons. J. Cell. Biochem., 101, 566-575. Cheng D, Hoogenraad CC, Rush J, et al. (2006) Relative and absolute quantification of postsynaptic density proteome isolated from rat forebrain and cerebellum. Mol Cell Proteomics, 5, 1158-70. Cheng HY, Hsieh MT, Wu CR, et al. (2008) Schizandrin protects primary cultures of rat cortical cells from glutamate-induced excitotoxicity. J Pharmacol Sci, 107, 21-31. Cheskis BJ, Greger JG, Nagpal S, Freedman LP (2007) Signaling by estrogens. J Cell Physiol, 213, 610-7. Cline HT (2001) Dendritic arbor development and synaptogenesis. Curr. Opin. Neurobiol., 11, 118-126. Colbran RJ, Brown AM (2004) Calcium/calmodulin-dependent protein kinase II and synaptic plasticity. Curr Opin Neurobiol, 14, 318-27. Coleman KM, Smith CL (2001) Intracellular signaling pathways: nongenomic actions of estrogens and ligand-independent activation of estrogen receptors. Front Biosci, 6, D1379-91. Corbit KC, Foster DA, Rosner MR (1999) Protein kinase Cdelta mediates neurogenic but not mitogenic activation of mitogen-activated protein kinase in neuronal cells. Mol Cell Biol, 19, 4209-18. Cui S, Goldstein RS (2000) Expression of estrogen receptors in the dorsal root ganglia of the chick embryo. Brain Res, 882, 236-40. Czondor K, Ellwanger K, Fuchs YF, et al. (2009) Protein kinase D controls the integrity of Golgi apparatus and the maintenance of dendritic arborization in hippocampal neurons. Mol Biol Cell, 20, 2108-20. Dailey ME, Smith SJ (1996) The dynamics of dendritic structure in developing hippocampal slices. J. Neurosci., 16, 2983-2994. Danciu TE, Adam RM, Naruse K, Freeman MR, Hauschka PV (2003) Calcium regulates the PI3K-Akt pathway in stretched osteoblasts. FEBS Lett, 536, 193-7. Datta SR, Dudek H, Tao X, et al. (1997) Akt Phosphorylation of BAD Couples Survival Signals to the Cell-Intrinsic Death Machinery. Cell, 91, 231-241. Davies AM (2000) Neurotrophins: neurotrophic modulation of neurite growth. Curr Biol, 10, R198-200. de Wilde A, Heberden C, Chaumaz G, Bordat C, Lieberherr M (2006) Signaling networks from Gbeta1 subunit to transcription factors and actin remodeling via a membrane-located ERbeta-related protein in the rapid action of daidzein in osteoblasts. J Cell Physiol, 209, 786-801. Dhandapani KM, Brann DW (2002) Protective effects of estrogen and selective estrogen receptor modulators in the brain. Biology of Reproduction, 67, 1379-85. Dotti CG, Sullivan CA, Banker GA (1988) The establishment of polarity by hippocampal neurons in culture. J. Neurosci., 8, 1454-1468. Egashira N, Kurauchi K, Iwasaki K, et al. (2008) Schizandrin reverses memory impairment in rats. Phytother Res, 22, 49-52. Fagerstrom S, Pahlman S, Gestblom C, Nanberg E (1996) Protein kinase C-epsilon is implicated in neurite outgrowth in differentiating human neuroblastoma cells. Cell Growth Differentiation, 7, 775-785. Fernyhough P, Huang TJ, Verkhratsky A (2003) Mechanism of mitochondrial dysfunction in diabetic sensory neuropathy. J Peripher Nerv Syst, 8, 227-35. Fink CC, Bayer KU, Myers JW, Ferrell JE, Jr., Schulman H, Meyer T (2003) Selective regulation of neurite extension and synapse formation by the beta but not the alpha isoform of CaMKII. Neuron, 39, 283-297. Finkbeiner S (2000) CREB Couples Neurotrophin Signals to Survival Messages. Neuron, 25, 11-14. Fong DK, Rao A, Crump FT, Craig AM (2002) Rapid synaptic remodeling by protein kinase C: reciprocal translocation of NMDA receptors and calcium/calmodulin-dependent kinase II. J Neurosci, 22, 2153-64. Fritz JD, Swartz DR, Greaser ML (1989) Factors affecting polyacrylamide gel electrophoresis and electroblotting of high-molecular-weight myofibrillar proteins. Analyt. Biochem., 180, 205-210. Fu M, Sun ZH, Zong M, He XP, Zuo HC, Xie ZP (2008) Deoxyschisandrin modulates synchronized Ca2+ oscillations and spontaneous synaptic transmission of cultured hippocampal neurons. Acta Pharmacol Sin, 29, 891-8. Furuya F, Guigon CJ, Zhao L, Lu C, Hanover JA, Cheng SY (2007) Nuclear receptor corepressor is a novel regulator of phosphatidylinositol 3-kinase signaling. Mol Cell Biol, 27, 6116-26. Gao Y, Deng K, Hou J, et al. (2004) Activated CREB is sufficient to overcome inhibitors in myelin and promote spinal axon regeneration in vivo. Neuron, 44, 609-21. Gao Y, Nikulina E, Mellado W, Filbin MT (2003) Neurotrophins elevate cAMP to reach a threshold required to overcome inhibition by MAG through extracellular signal-regulated kinase-dependent inhibition of phosphodiesterase. Journal of Neuroscience, 23, 11770-11777. Gikas PD, Mokbel K (2005) Phytoestrogens and the risk of breast cancer: a review of the literature. Int J Fertil Womens Med, 50, 250-8. Goldberg JL (2003) How does an axon grow? Genes Dev, 17, 941-58. Goldin M, Segal M (2003) Protein kinase C and ERK involvement in dendritic spine plasticity in cultured rodent hippocampal neurons. Eur. J. Neurosci., 17, 2529-2539. Goldshmit Y, Walters CE, Scott HJ, Greenhalgh CJ, Turnley AM (2004) SOCS2 induces neurite outgrowth by regulation of epidermal growth factor receptor activation. J Biol Chem, 279, 16349-55. Gu L, Laly M, Chang HC, et al. (2005) Isoflavone conjugates are underestimated in tissues using enzymatic hydrolysis. J Agric Food Chem, 53, 6858-63. Gundimeda U, McNeill TH, Schiffman JE, Hinton DR, Gopalakrishna R (2010) Green tea polyphenols potentiate the action of nerve growth factor to induce neuritogenesis: possible role of reactive oxygen species. J Neurosci Res, 88, 3644-55. Hamalainen M, Nieminen R, Vuorela P, Heinonen M, Moilanen E (2007) Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-kappaB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-kappaB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Mediators Inflamm, 2007, 45673. Harada T, Morooka T, Ogawa S, Nishida E (2001) ERK induces p35, a neuron-specific activator of Cdk5, through induction of Egr1. Nat Cell Biol, 3, 453-9. Hardingham GE, Chawla S, Johnson CM, Bading H (1997) Distinct functions of nuclear and cytoplasmic calcium in the control of gene expression. Nature, 385, 260-265. Harris KM, Kater SB (1994) Dendritic spines: cellular specializations imparting both stability and flexibility to synaptic function. Annu. Rev. Neurosci., 17, 341-371. He Z, Koprivica V (2004) The Nogo signaling pathway for regeneration block. Annu Rev Neurosci, 27, 341-68. Hedelin M, Lof M, Olsson M, Adlercreutz H, Sandin S, Weiderpass E (2008) Dietary phytoestrogens are not associated with risk of overall breast cancer but diets rich in coumestrol are inversely associated with risk of estrogen receptor and progesterone receptor negative breast tumors in Swedish women. J Nutr, 138, 938-45. Heldring N, Pike A, Andersson S, et al. (2007) Estrogen Receptors: How Do They Signal and What Are Their Targets. Physiological Reviews, 87, 905. Hsieh MT, Tsai ML, Peng WH, Wu CR (1999) Effects of Fructus schizandrae on cycloheximide-induced amnesia in rats. Phytother. Res., 13, 256-257. Hsieh MT, Wu CR, Wang WH, Lin LW (2001) The ameliorating effect of the water layer of Fructus Schisandrae on cycloheximide-induced amnesia in rats: interaction with drugs acting at neurotransmitter receptors. Pharmacol Res, 43, 17-22. Hung TM, Na M, Min BS, et al. (2007) Acetylcholinesterase inhibitory effect of lignans isolated from Schizandra chinensis. Arch Pharm Res, 30, 685-90. Hwang CS, Kwak HS, Lim HJ, et al. (2006) Isoflavone metabolites and their in vitro dual functions: they can act as an estrogenic agonist or antagonist depending on the estrogen concentration. J Steroid Biochem Mol Biol, 101, 246-53. Ikeya Y, Taguchi H, Yosioka I, Kobayashi H (1979) The constituents of Schizandra chinensis Baill. I. Isolation and structure determination of five new lignans, gomisin A, B, C, F and G, and the absolute structure of schizandrin. Chem Pharm Bull (Tokyo), 27, 1383-94. Jeong DG, Park WK, Park S (2008) Artemin activates axonal growth via SFK and ERK-dependent signalling pathways in mature dorsal root ganglia neurons. Cell Biochem Funct, 26, 210-20. Jin Y, Wu H, Cohen EM, et al. (2007) Genistein and daidzein induce neurotoxicity at high concentrations in primary rat neuronal cultures. J Biomed Sci, 14, 275-84. Jung CH, Hong MH, Kim JH, et al. (2007) Protective effect of a phenolic-rich fraction from Schisandra chinensis against H2O2-induced apoptosis in SH-SY5Y cells. J. Pharm. Pharmacol., 59, 455-462. Kang SY, Lee KY, Koo KA, et al. (2005a) ESP-102, a standardized combined extract of Angelica gigas, Saururus chinensis and Schizandra chinensis, significantly improved scopolamine-induced memory impairment in mice. Life Sci., 76, 1691-1705. Kang SY, Lee KY, Koo KA, et al. (2005b) ESP-102, a standardized combined extract of Angelica gigas, Saururus chinensis and Schizandra chinensis, significantly improved scopolamine-induced memory impairment in mice. Life Sci, 76, 1691-705. Kennedy MB (2000) Signal-processing machines at the postsynaptic density. Science, 290, 750-754. Kida S, Josselyn SA, de Ortiz SP, et al. (2002) CREB required for the stability of new and reactivated fear memories. Nat. Neurosci., 5, 348-355. Kim SH, Joo MH, Yoo SH (2009) Structural identification and antioxidant properties of major anthocyanin extracted from Omija (Schizandra chinensis) fruit. J Food Sci, 74, C134-40. Kim SR, Lee MK, Koo KA, et al. (2004a) Dibenzocyclooctadiene lignans from Schisandra chinensis protect primary cultures of rat cortical cells from glutamate-induced toxicity. J. Neurosci. Res, 76, 397-405. Kim SR, Lee MK, Koo KA, et al. (2004b) Dibenzocyclooctadiene lignans from Schisandra chinensis protect primary cultures of rat cortical cells from glutamate-induced toxicity. J Neurosci Res, 76, 397-405. Ko KM, Lam BY (2002) Schisandrin B protects against tert-butylhydroperoxide induced cerebral toxicity by enhancing glutathione antioxidant status in mouse brain. Mol Cell Biochem, 238, 181-6. Kolkova K, Stensman H, Berezin V, Bock E, Larsson C (2005) Distinct roles of PKC isoforms in NCAM-mediated neurite outgrowth. J. Neurochem., 92, 886-894. Konur S, Ghosh A (2005) Calcium signaling and the control of dendritic development. Neuron, 46, 401-5. Kuiper GG, Lemmen JG, Carlsson B, et al. (1998) Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology, 139, 4252-63. Laabs T, Carulli D, Fawcett JW, Geller HM (2005) Chondroitin sulfate proteoglycans in neural development and regeneration. Current Opinion in Neurobiology, 15, 116-120. Lee SJ, Campomanes CR, Sikat PT, Greenfield AT, Allen PB, McEwen BS (2004) Estrogen induces phosphorylation of cyclic AMP response element binding (pCREB) in primary hippocampal cells in a time-dependent manner. Neurosci., 124, 549-560. Lephart ED, Porter JP, Hedges DW, Lund TD, Setchell KD (2004) Phytoestrogens: implications in neurovascular research. Current neurovascular research, 1, 455-64. Li QM, Fu YM, Shan ZY, et al. (2009) MSCs guide neurite directional extension and promote oligodendrogenesis in NSCs. Biochem Biophys Res Commun, 384, 372-7. Liu W, Yu R, Wu JH, Luo HM (2006a) Gamma-Schisandrin inhibits production of amyloid beta-protein 42 in M146L cells. Yao Xue Xue Bao, 41, 1136-1140. Liu W, Yu R, Wu JH, Luo HM (2006b) [Gamma-Schisandrin inhibits production of amyloid beta-protein 42 in M146L cells]. Yao Xue Xue Bao, 41, 1136-40. Lonze BE, Ginty DD (2002a) Function and regulation of CREB family transcription factors in the nervous system. Neuron, 35, 605-623. Lonze BE, Ginty DD (2002b) Function and regulation of CREB family transcription factors in the nervous system. Neuron, 35, 605-23. Lu CL, Hsieh JC, Dun NJ, et al. (2009) Estrogen rapidly modulates 5-hydroxytrytophan-induced visceral hypersensitivity via GPR30 in rats. Gastroenterology, 137, 1040-50. Ma CJ, Kim SH, Lee KY, et al. (2009) ESP-102, a combined extract of Angelica gigas, Saururus chinensis and Schizandra chinensis, protects against glutamate-induced toxicity in primary cultures of rat cortical cells. Phytother Res, 23, 1587-91. Ma TC, Campana A, Lange PS, et al. (2010) A large-scale chemical screen for regulators of the arginase 1 promoter identifies the soy isoflavone daidzeinas a clinically approved small molecule that can promote neuronal protection or regeneration via a cAMP-independent pathway. J Neurosci, 30, 739-48. Maejima Y, Ueba H, Kuroki M, et al. (2003) Src family kinases and nitric oxide production are required for hepatocyte growth factor-stimulated endothelial cell growth. Atherosclerosis, 167, 89-95. Manthey D, Behl C (2006) From structural biochemistry to expression profiling: neuroprotective activities of estrogen. Neuroscience, 138, 845-50. Markus A, Patel TD, Snider WD (2002a) Neurotrophic factors and axonal growth. Curr Opin Neurobiol, 12, 523-31. Markus A, Zhong J, Snider WD (2002b) Raf and akt mediate distinct aspects of sensory axon growth. Neuron, 35, 65-76. McAllister AK (2000) Cellular and molecular mechanisms of dendrite growth. Cereb Cortex, 10, 963-73. Menegon A, Verderio C, Leoni C, et al. (2002) Spatial and temporal regulation of Ca2+/calmodulin-dependent protein kinase II activity in developing neurons. J. Neurosci., 22, 7016-7026. Mhyre AJ, Dorsa DM (2006) Estrogen activates rapid signaling in the brain: role of estrogen receptor alpha and estrogen receptor beta in neurons and glia. Neuroscience, 138, 851-8. Miller DR, Lee GM, Maness PF (1993) Increased neurite outgrowth induced by inhibition of protein tyrosine kinase activity in PC12 pheochromocytoma cells. J Neurochem, 60, 2134-44. Minano A, Xifro X, Perez V, Barneda-Zahonero B, Saura CA, Rodriguez-Alvarez J (2008) Estradiol facilitates neurite maintenance by a Src/Ras/ERK signalling pathway. Mol Cell Neurosci, 39, 143-51. Mu Y, Zhang J, Zhang S, et al. (2006) Traditional Chinese medicines Wu Wei Zi (Schisandra chinensis Baill) and Gan Cao (Glycyrrhiza uralensis Fisch) activate pregnane X receptor and increase warfarin clearance in rats. J Pharmacol Exp Ther, 316, 1369-77. Nakhost A, Kabir N, Forscher P, Sossin WS (2002) Protein kinase C isoforms are translocated to microtubules in neurons. J. Biol. Chem., 277, 40633-40639. Neumann S, Woolf CJ (1999) Regeneration of dorsal column fibers into and beyond the lesion site following adult spinal cord injury. Neuron, 23, 83-91. Nielsen IL, Williamson G (2007) Review of the factors affecting bioavailability of soy isoflavones in humans. Nutr Cancer, 57, 1-10. Nishida H, Tatewaki N, Nakajima Y, et al. (2009) Inhibition of ATR protein kinase activity by schisandrin B in DNA damage response. Nucleic Acids Res, 37, 5678-89. Nishiyama N, Wang YL, Saito H (1995) Beneficial effects of S-113m, a novel herbal prescription, on learning impairment model in mice. Biol. Pharm. Bull., 18, 1498-1503. Obata K, Yamanaka H, Dai Y, et al. (2004) Differential activation of MAPK in injured and uninjured DRG neurons following chronic constriction injury of the sciatic nerve in rats. Eur J Neurosci, 20, 2881-95. Ososki AL, Kennelly EJ (2003) Phytoestrogens: a review of the present state of research. Phytotherapy research : PTR, 17, 845-69. Pao TT, Hsu KF, Liu KT, Chang LG, Chuang CH, Sung CY (1977) Protective action of schizandrin B on hepatic injury in mice. Chin Med J (Engl), 3, 173-9. Papka RE, Storey-Workley M (2002) Estrogen receptor-alpha and -beta coexist in a subpopulation of sensory neurons of female rat dorsal root ganglia. Neurosci Lett, 319, 71-4. Patrone C, Andersson S, Korhonen L, Lindholm D (1999) Estrogen receptor-dependent regulation of sensory neuron survival in developing dorsal root ganglion. Proc Natl Acad Sci USA, 96, 10905-10. Ping SE, Trieu J, Wlodek ME, Barrett GL (2008) Effects of estrogen on basal forebrain cholinergic neurons and spatial learning. J Neurosci Res, 86, 1588-98. Polleux F, Giger RJ, Ginty DD, Kolodkin AL, Ghosh A (1998) Patterning of cortical efferent projections by semaphorin-neuropilin interactions. Science, 282, 1904-6. Polleux F, Morrow T, Ghosh A (2000) Semaphorin 3A is a chemoattractant for cortical apical dendrites. Nature, 404, 567-73. Prekeris R, Mayhew MW, Cooper JB, Terrian DM (1996) Identification and localization of an actin-binding motif that is unique to the epsilon isoform of protein kinase C and participates in the regulation of synaptic function. J. Cell Biol., 132, 77-90. Prokai L, Simpkins JW (2007) Structure-nongenomic neuroprotection relationship of estrogens and estrogen-derived compounds. Pharmacology & therapeutics, 114, 1-12. Read DE, Gorman AM (2009) Involvement of Akt in neurite outgrowth. Cell Mol Life Sci, 66, 2975-84. Redmond L, Ghosh A (2005) Regulation of dendritic development by calcium signaling. Cell Calcium, 37, 411-416. Runyan SA, Phelps PE (2009) Mouse olfactory ensheathing glia enhance axon outgrowth on a myelin substrate in vitro. Exp Neurol, 216, 95-104. Saito N, Itouji A, Totani Y, et al. (1993) Cellular and intracellular localization of epsilon-subspecies of protein kinase C in the rat brain; presynaptic localization of the epsilon-subspecies. Brain Res., 607, 241-248. Sakaue Y, Sanada M, Sasaki T, Kashiwagi A, Yasuda H (2003) Amelioration of retarded neurite outgrowth of dorsal root ganglion neurons by overexpression of PKCdelta in diabetic rats. Neuroreport, 14, 431-6. Schreihofer DA (2005) Transcriptional regulation by phytoestrogens in neuronal cell lines. Mol Cell Endocrinol, 231, 13-22. Scoville SA, Bufton SM, Liuzzi FJ (1997) Estrogen regulates neurofilament gene expression in adult female rat dorsal root ganglion neurons. Exp Neurol, 146, 596-9. Segars JH, Driggers PH (2002) Estrogen action and cytoplasmic signaling cascades. Part I: membrane-associated signaling complexes. Trends Endocrinol Metab, 13, 349-54. Seo TB, Baek K, Kwon KB, et al. (2009) Shengmai-san-mediated enhancement of regenerative responses of spinal cord axons after injury in rats. J Pharmacol Sci, 110, 483-92. Shaywitz AJ, Greenberg ME (1999) CREB: A stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annual Review of Biochemistry, 68, 821-861. Sheng M (2001) Molecular organization of the postsynaptic specialization. Proc. Natl. Acad. Sci. U.S.A. , 98, 7058-7061. Sjogreen B, Wiklund P, Ekstrom PA (2000) Mitogen activated protein kinase inhibition by PD98059 blocks nerve growth factor stimulated axonal outgrowth from adult mouse dorsal root ganglia in vitro. Neuroscience, 100, 407-16. Sohrabji F, Miranda RC, Toran-Allerand CD (1994) Estrogen differentially regulates estrogen and nerve growth factor receptor mRNAs in adult sensory neurons. J Neurosci, 14, 459-71. Spector JG, Lee P, Derby A (2000) Rabbit facial nerve regeneration in autologous nerve grafts after antecedent injury. Laryngoscope, 110, 660-7. Spencer TK, Mellado W, Filbin MT (2008) BDNF activates CaMKIV and PKA in parallel to block MAG-mediated inhibition of neurite outgrowth. Mol Cell Neurosci, 38, 110-6. Takanami K, Sakamoto H, Matsuda K, et al. (2010) Expression of G protein-coupled receptor 30 in the spinal somatosensory system. Brain Res, 1310, 17-28. Thompson DM, Buettner HM (2006) Neurite outgrowth is directed by schwann cell alignment in the absence of other guidance cues. Ann Biomed Eng, 34, 161-8. Thorp AA, Sinn N, Buckley JD, Coates AM, Howe PR (2009) Soya isoflavone supplementation enhances spatial working memory in men. Br J Nutr, 102, 1348-54. Tsuji M, Inanami O, Kuwabara M (2001) Induction of neurite outgrowth in PC12 cells by alpha -phenyl-N-tert-butylnitron through activation of protein kinase C and the Ras-extracellular signal-regulated kinase pathway. J. Biol. Chem., 276, 32779-32785. Tucker BA, Rahimtula M, Mearow KM (2008) Src and FAK are key early signalling intermediates required for neurite growth in NGF-responsive adult DRG neurons. Cell Signal, 20, 241-57. Vaillant AR, Zanassi P, Walsh GS, Aumont A, Alonso A, Miller FD (2002) Signaling mechanisms underlying reversible, activity-dependent dendrite formation. Neuron, 34, 985-998. Vanhaesebroeck B, Guillermet-Guibert J, Graupera M, Bilanges B (2010) The emerging mechanisms of isoform-specific PI3K signalling. Nat Rev Mol Cell Biol, 11, 329-41. Verheus M, van Gils CH, Keinan-Boker L, Grace PB, Bingham SA, Peeters PH (2007) Plasma phytoestrogens and subsequent breast cancer risk. J Clin Oncol, 25, 648-55. Wang B, Wang XM (2009) Schisandrin B protects rat cortical neurons against Abeta1-42-induced neurotoxicity. Pharmazie, 64, 450-4. Wang CN, Chi CW, Lin YL, Chen CF, Shiao YJ (2001) The neuroprotective effects of phytoestrogens on amyloid beta protein-induced toxicity are mediated by abrogating the activation of caspase cascade in rat cortical neurons. J Biol Chem, 276, 5287-95. Wang KC, Koprivica V, Kim JA, et al. (2002) Oligodendrocyte-myelin glycoprotein is a Nogo receptor ligand that inhibits neurite outgrowth. Nature, 417, 941-944. Wang P, Jeng CJ, Chien CL, Wang SM (2008) Signaling mechanisms of daidzein-induced axonal outgrowth in hippocampal neurons. Biochem Biophys Res Commun, 366, 393-400. Wayman GA, Impey S, Marks D, et al. (2006) Activity-dependent dendritic arborization mediated by CaM-kinase I activation and enhanced CREB-dependent transcription of Wnt-2. Neuron, 50, 897-909. Wu GY, Cline HT (1998) Stabilization of dendritic arbor structure in vivo by CaMKII. Science, 279, 222-226. Wu GY, Deisseroth K, Tsien RW (2001) Spaced stimuli stabilize MAPK pathway activation and its effects on dendritic morphology. Nat. Neurosci., 4, 151-158. Yang SH, Chen YJ, Tung PY, et al. (2008) Anti-Thy-1 antibody-induced neurite outgrowth in cultured dorsal root ganglionic neurons is mediated by the c-Src-MEK signaling pathway. J Cell Biochem, 103, 67-77. Yang T, Yin W, Derevyanny VD, Moore LA, Longo FM (2005) Identification of an ectodomain within the LAR protein tyrosine phosphatase receptor that binds homophilically and activates signalling pathways promoting neurite outgrowth. Eur J Neurosci, 22, 2159-70. Yiu G, He Z (2006) Glial inhibition of CNS axon regeneration. Nat Rev Neurosci, 7, 617-27. Zeng S, Tai F, Zhai P, Yuan A, Jia R, Zhang X (2010) Effect of daidzein on anxiety, social behavior and spatial learning in male Balb/cJ mice. Pharmacol Biochem Behav, 96, 16-23. Zhao L, Chen Q, Diaz Brinton R (2002) Neuroprotective and neurotrophic efficacy of phytoestrogens in cultured hippocampal neurons. Exp Biol Med (Maywood), 227, 509-19. Ziv NE, Smith SJ (1996) Evidence for a role of dendritic filopodia in synaptogenesis and spine formation. Neuron, 17, 91-102. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10241 | - |
dc.description.abstract | 神經細胞依賴神經軸突(axon)與樹突(dendrite)來達成彼此間的聯繫。神經系統的疾病,包括中風、外傷等,常會造成軸突與樹突的傷害與萎縮,導致運動、感覺、語言等神經功能障礙。如何能夠促進神經胞突的生長,一直是基礎與臨床神經醫學的重要課題。我們的研究係以體外培養的大鼠神經元細胞為實驗對象,尋找能夠促進神經胞突生長的分子,與探討其訊息傳遞機轉。研究的第一部份,使用中藥草常用的五味子(Schisandra chinensis)所含的一個主要成分:五味子醇(schisandrin),觀察其對背根神經節神經元細胞(dorsal root ganglion neuron, DRG neurons)與海馬迴神經元細胞(hippocampal neurons)的效果。我們發現五味子醇作用24小時後,能夠促進 DRG neurons 的胞突延長伸展與分枝,且在濃度為 3 μg/ml 時的效果最好。而 3 μg/ml 的五味子醇對於大型或小型的 DRG neurons 皆有增加胞突生長的效果。因此進一步分析五味子醇促進 DRG neurons 胞突增生的機轉,在加入五味子醇前使用 KT5720(PKA 抑制劑)、PD98059(MEK 抑制劑)、或 LY294002(PI3K抑制劑)等激酶抑制劑,發現僅有 LY294002 能夠抑制胞突增生。而以西方點墨法分析激酶活化情形,發現五味子醇作用 15 分鐘後能促進 PI3K 與 Akt 的磷酸化;LY294002 預先處理則會抑制磷酸化 Akt 激酶的增加。五味子醇也增加 CREB 蛋白的磷酸化,且同樣會受到 LY294002 預先處理的抑制。綜合以上的結果,我們推論五味子醇係透過 PI3K-Akt-CREB 的訊息傳遞路徑來促進 DRG neuron 的胞突增生。另一方面,我們亦發現五味子醇能夠促進 hippocampal neurons 的胞突伸展與分枝。進一步分析五味子醇促進 hippocampal neurons 胞突增生的機轉,在加入五味子醇前使用 KN93(CaMKII 抑制劑)、εV1-2(PKCε抑制劑)或 PD98059(MEK 抑制劑)均能夠抑制神經胞突增生。使用 Fluo-3 AM 染色,顯示加入 五味子醇十分鐘後會使細胞內鈣離子濃度上升。以西方點墨法分析,發現五味子醇作用 15 分鐘後能促進 CaMKII, PKCε, MEK 與 CREB 蛋白的磷酸化(活化);KN93 會抑制因五味子醇引起的 PKCε 激酶活化、εV1-2 會抑制 MEK 激酶的活化、PD98059 則會抑制磷酸化 CREB 蛋白的增加。綜合以上的結果,我們推論五味子醇會使鈣離子進入 hippocampal neuron,然後啓動 CaMKII-PKCε-MEK-CREB 的訊息傳遞路徑來促進神經胞突增生。另外,五味子醇也會使海馬迴神經細胞的 post-synaptic protein 95 (PSD-95) 表達增加,與促進細胞攝入 FM1-43,顯示五味子醇亦具有促進海馬迴神經細胞突觸增生(synaptogenesis)的效果。
研究的第二部份,針對大豆異黃酮(isoflavone)的一個主要成分:大豆甙元(daidzein),分析它對於背根神經節神經元細胞(dorsal root ganglion neuron, DRG neurons)的效果。我們發現大豆甙元作用24小時後,能夠促進 DRG neurons 的胞突延長伸展與分枝,且在濃度為 30 μM 時的效果最好。而 30 μM 的大豆甙元對於大型或小型的 DRG neurons 皆有增加胞突生長的效果。進一步分析大豆甙元促進 DRG neurons 胞突增生的機轉,在加入大豆甙元前使用 ICI 182780(estrogen receptor α/β 拮抗劑)或 G15(membrane estrogen receptor GPR-30 拮抗劑),皆無抑制胞突增生的效果,顯示大豆甙元雖為植物雌激素的一種,但並非透過雌激素接受器使 DRG neuron 的胞突增生。而以 PP2(Src 抑制劑)、staurosporin(泛 PKC 抑制劑)、rottlerin(PKCδ 抑制劑)、U0126(MEK 抑制劑)等激酶抑制劑預先處理,則能夠抑制大豆甙元引起的胞突增生。而以西方點墨法分析激酶活化情形,發現大豆甙元作用後能促進 Src, PKCδ, ERK 的磷酸化(活化);PP2 會抑制因大豆甙元引起 PKCδ 激酶的磷酸化,rottlerin 則會抑制磷酸化 ERK 激酶的增加。歸納以上的結果,我們推論大豆甙元係透過 Src-PKCδ-MEK/ERK 的訊息傳遞路徑來促進 DRG neuron 的胞突增生。 總結我們的研究結果,顯示五味子醇與大豆甙元均有促進神經細胞胞突生長的作用,並透過不同的訊息路徑來完成反應。而五味子醇對於背根神經元與海馬神經元細胞皆能促進胞突增生,但亦經由不同的訊息傳遞方式來達成。 | zh_TW |
dc.description.abstract | Neurons in different location require axons and dendrites to communicate with each other. Neurological diseases, e.g. stroke, head trauma, and spinal cord injury, often damage axons and dendrites and result in deficits of motor, sensory and language functions. How to promote growth of neuronal processes (neurites) is a major issue in the field of basic and clinical neuroscience. Our researches utilized in vitro cultured rat neurons to search for potential neuritogenic molecules and its signaling mechanisms. The first part of the research focused on schisandrin, a major ingredient of the Chinese herb Schisandra chinensis, and studied its effect on dorsal root ganglion (DRG) and hippocampal neurons. After treatment with schisandrin for 24 hours, DRG neurons showed increased lengthening and branching of neurites, and maximal effect was seen at a concentration of 3 μg/ml. Both large and small DRG neurons responded to schisandrin. To study the signaling pathway, KT5720 (PKA inhibitor), PD98059 (MEK inhibitor), or LY294002 (PI3K inhibitor) were applied before schisandrin treatment in DRG neurons, and only LY294002 blocked the neuritogenic effect of schisandrin. Western blot analysis showed that schisandrin enhanced phosphorylation of PI3K and Akt, which were blocked by pretreatment of LY294002. Schisandrin also increased phosphorylation of CREB, which was also inhibited by pretreatment of LY294002. Therefore, schisandrin activated PI3K-Akt-CREB pathway to enhance neurite outgrowth. Besides neuritogenic effect on DRG neurons, schisandrin also increased neurite length and branching complexity in hippocampal neurons. This effect was reversed by pretreatment with KN93 (CaMKII inhibitor), εV1-2 (PKCε inhibitor), or PD98059 (MEK inhibitor). Schisandrin also induced calcium inflow into hippocampal neurons in 10 minutes. Western blot showed that schisandrin activated CaMKII, PKCε, MEK and CREB, and the activation of schisandrin-induced PKCε, MEK, and CREB were blocked by pretreatment with KN93, εV1-2, and PD98059, respectively. The result indicated schisandrin caused calcium inflow and activated CaMKII-PKCε-MEK-CREB pathway to increase neuritogenesis. In addition, schisandrin increased expression of post-synaptic protein 95 and uptake of FM1-43, suggesting its role in synaptogenesis of hippocampal neurons.
The second part of this study focused on daidzein, a major component of isoflavone. Following treatment of daidzein for 24 hours, both small and large DRG neurons demonstrated increased lengthening and branching of neurites, and maximal effect occurred at 30 μM. Despite structural similarity of daidzein to estrogen, ICI 182780 (estrogen α/β receptor inhibitor) and G15 (membrane estrogen receptor GPR-30) failed to inhibit the neuritogenic effect of daidzein, indicating that daidzein did not stimulate neurite outgrowth via the estrogen receptors. On the other hand, PP2 (Src inhibitor), staurosporin (pan-PKC inhibitor), rottlerin (PKCδ inhibitor), and U0126 (MEK inhibitor) pretreatment abolished the neuritogenic response of DRG neurons to daidzein. Increased phosphorylation of Src, PKCδ, and MEK occurred after daidzein treatment, and activation of the latter two kinases were blocked by PP2 and rottlerin respectively. Therefore, daidzein activated Src-PKCδ-ERK pathway, which led to neuritogenesis in DRG neurons. In conclusion, the results of our research demonstrate that both schisandrin and daidzein have neuritogenic effect, and act by different signaling mechanisms. Schisandrin enhanced neurite outgrowth of both DRG and hippocampal neurons, also via different pathways of signal transduction. | en |
dc.description.provenance | Made available in DSpace on 2021-05-20T21:13:13Z (GMT). No. of bitstreams: 1 ntu-100-D91446003-1.pdf: 28010218 bytes, checksum: 2e31b2ebefae0f25dca55ab9d30aa053 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 口試委員會審定書…………………………………………………. i
誌謝…………………………………………………………………. iii 中文摘要……….............…………………………………………. v 英文摘要……………………..........………………………………. ix Chapter 1. Literature review…..………………………………. 1 Chapter 2. Study on the effect and mechanism of schisandrin for neurite outgrowth in primarily cultured rat dorsal root ganglion neurons Introduction…………………………………………………. 5 Materials and methods……..……………………………. 14 Discussion..…………………………………………………. 17 Chapter 3. Study on the effect and mechanism of schisandrin for neuritogenesis and synaptogenesis in primarily cultured rat hippocampal neurons Introduction…...……………………………………………. 29 Materials and method.……………………………………. 32 Results…….…………………………………………………. 37 Discussion..…………………………………………………. 40 Chapter 4. Study on the effect and mechanism of daidzein for neuritogenesis inprimarily cultured rat dorsal root ganglion neurons Introduction...………………………………………………. 51 Materials and methods…...………………………………. 55 Results….....…………………………………………………. 59 Discussion……………………………………………………. 62 Chapter 5. Conclusion and future perspective.……………. 79 References……..........……………………………………………. 83 附錄.………….………………………………………………………. 97 | |
dc.language.iso | en | |
dc.title | 五味子醇與大豆甙元對於促進神經細胞胞突生長的作用與其機轉之探討 | zh_TW |
dc.title | Study on the Effect and Mechanism of Schisandrin and Daidzein for Neurite Outgrowth in Primarily Cultured Neuronal Cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-1 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 謝松蒼,陳玉怜,鄭瓊娟,王懷詩,呂俊宏 | |
dc.subject.keyword | 五味子醇,大豆甙,元,背根神經節神經細胞,海馬迴神經細胞,神經胞突增生,神經突觸增生,訊息傳遞, | zh_TW |
dc.subject.keyword | schisandrin,daidzein,dorsal root ganglion neuron,hippocampal neuron,neurite outgrowth,synaptogenesis,signal transduction, | en |
dc.relation.page | 99 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2011-02-11 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 解剖學暨生物細胞學研究所 | zh_TW |
顯示於系所單位: | 解剖學暨細胞生物學科所 |
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