請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/102077完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 許弘明 | zh_TW |
| dc.contributor.advisor | Hong-Ming Hsu | en |
| dc.contributor.author | 廖偉捷 | zh_TW |
| dc.contributor.author | Wei-Chieh Liao | en |
| dc.date.accessioned | 2026-03-13T16:13:58Z | - |
| dc.date.available | 2026-03-14 | - |
| dc.date.copyright | 2026-03-13 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-12-04 | - |
| dc.identifier.citation | 1. Cotch, M.F., et al., Trichomonas vaginalis associated with low birth weight and preterm delivery. The Vaginal Infections and Prematurity Study Group. Sex Transm Dis, 1997. 24(6): p. 353-60.
2. Gerbase, A.C., et al., Global prevalence and incidence estimates of selected curable STDs. Sex Transm Infect, 1998. 74 Suppl 1: p. S12-6. 3. Leitsch, D., Recent Advances in the Trichomonas vaginalis Field. F1000Res, 2016. 5. 4. Shafir, S.C., F.J. Sorvillo, and L. Smith, Current issues and considerations regarding trichomoniasis and human immunodeficiency virus in African-Americans. Clin Microbiol Rev, 2009. 22(1): p. 37-45, Table of Contents. 5. Stark, J.R., et al., Prospective study of Trichomonas vaginalis infection and prostate cancer incidence and mortality: Physicians' Health Study. J Natl Cancer Inst, 2009. 101(20): p. 1406-11. 6. Zhang, Z.F. and C.B. Begg, Is Trichomonas vaginalis a cause of cervical neoplasia? Results from a combined analysis of 24 studies. Int J Epidemiol, 1994. 23(4): p. 682-90. 7. Huang, S.H., et al., Prevalence, Associated Factors, and Appropriateness of Empirical Treatment of Trichomoniasis, Bacterial Vaginosis, and Vulvovaginal Candidiasis among Women with Vaginitis. Microbiol Spectr, 2023. 11(3): p. e0016123. 8. Dunne, R.L., et al., Drug resistance in the sexually transmitted protozoan Trichomonas vaginalis. Cell Res, 2003. 13(4): p. 239-49. 9. Hirt, R.P., Trichomonas vaginalis virulence factors: an integrative overview. Sex Transm Infect, 2013. 89(6): p. 439-43. 10. Arroyo, R., et al., Signalling of Trichomonas vaginalis for amoeboid transformation and adhesion synthesis follows cytoadherence. Mol Microbiol, 1993. 7(2): p. 299-309. 11. Shiratori, M., et al., Persistent Trichomonas vaginalis infections and the pseudocyst form. Trends Parasitol, 2023. 39(12): p. 1023-1031. 12. Arroyo, R., J. Engbring, and J.F. Alderete, Molecular basis of host epithelial cell recognition by Trichomonas vaginalis. Mol Microbiol, 1992. 6(7): p. 853-62. 13. Hirt, R.P., et al., Trichomonas vaginalis pathobiology new insights from the genome sequence. Adv Parasitol, 2011. 77: p. 87-140. 14. Lama, A., et al., Glyceraldehyde-3-phosphate dehydrogenase is a surface-associated, fibronectin-binding protein of Trichomonas vaginalis. Infect Immun, 2009. 77(7): p. 2703-11. 15. Kummer, S., et al., Induction of human host cell apoptosis by Trichomonas vaginalis cysteine proteases is modulated by parasite exposure to iron. Microb Pathog, 2008. 44(3): p. 197-203. 16. Sommer, U., et al., Identification of Trichomonas vaginalis cysteine proteases that induce apoptosis in human vaginal epithelial cells. J Biol Chem, 2005. 280(25): p. 23853-60. 17. Quan, J.H., et al., Trichonomas vaginalis metalloproteinase induces apoptosis of SiHa cells through disrupting the Mcl-1/Bim and Bcl-xL/Bim complexes. PLoS One, 2014. 9(10): p. e110659. 18. Alderete, J.F., D. Provenzano, and M.W. Lehker, Iron mediates Trichomonas vaginalis resistance to complement lysis. Microb Pathog, 1995. 19(2): p. 93-103. 19. Provenzano, D. and J.F. Alderete, Analysis of human immunoglobulin-degrading cysteine proteinases of Trichomonas vaginalis. Infect Immun, 1995. 63(9): p. 3388-95. 20. Mendoza-Lopez, M.R., et al., CP30, a cysteine proteinase involved in Trichomonas vaginalis cytoadherence. Infect Immun, 2000. 68(9): p. 4907-12. 21. Midlej, V. and M. Benchimol, Trichomonas vaginalis kills and eats--evidence for phagocytic activity as a cytopathic effect. Parasitology, 2010. 137(1): p. 65-76. 22. Mandal, K., Review of PIP2 in Cellular Signaling, Functions and Diseases. Int J Mol Sci, 2020. 21(21). 23. Oude Weernink, P.A., M. Schmidt, and K.H. Jakobs, Regulation and cellular roles of phosphoinositide 5-kinases. Eur J Pharmacol, 2004. 500(1-3): p. 87-99. 24. van Rheenen, J., et al., EGF-induced PIP2 hydrolysis releases and activates cofilin locally in carcinoma cells. J Cell Biol, 2007. 179(6): p. 1247-59. 25. Levental, I., et al., Calcium-dependent lateral organization in phosphatidylinositol 4,5-bisphosphate (PIP2)- and cholesterol-containing monolayers. Biochemistry, 2009. 48(34): p. 8241-8. 26. Nishizuka, Y., The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature, 1984. 308(5961): p. 693-8. 27. Cohen, G., et al., Crosstalk between protein kinase A and C regulates phospholipase D and F-actin formation during sperm capacitation. Dev Biol, 2004. 267(1): p. 230-41. 28. Berridge, M.J. and R.F. Irvine, Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature, 1984. 312(5992): p. 315-21. 29. Walensky, L.D. and S.H. Snyder, Inositol 1,4,5-trisphosphate receptors selectively localized to the acrosomes of mammalian sperm. J Cell Biol, 1995. 130(4): p. 857-69. 30. Antonescu, C.N., et al., Phosphatidylinositol-(4,5)-bisphosphate regulates clathrin-coated pit initiation, stabilization, and size. Mol Biol Cell, 2011. 22(14): p. 2588-600. 31. Ling, K., et al., Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions. Nature, 2002. 420(6911): p. 89-93. 32. Delang, L., J. Paeshuyse, and J. Neyts, The role of phosphatidylinositol 4-kinases and phosphatidylinositol 4-phosphate during viral replication. Biochem Pharmacol, 2012. 84(11): p. 1400-8. 33. Chytla, A., et al., Plasma membrane and nuclear phosphatidylinositol 4,5-bisphosphate signalling in cancer. Lipids Health Dis, 2025. 24(1): p. 39. 34. Chen, Y.J., et al., PIP2 regulating calcium signal modulates actin cytoskeleton-dependent cytoadherence and cytolytic capacity in the protozoan parasite Trichomonas vaginalis. PLoS Pathog, 2023. 19(12): p. e1011891. 35. Wu, K.Y., et al., Iron triggers TvPI4P5K proteostasis and Arf-mediated cell membrane trafficking to regulate PIP(2) signaling crucial for multiple pathogenic activities of the parasitic protozoan Trichomonas vaginalis. mBio, 2025. 16(2): p. e0186424. 36. 林書帆, 陰道滴蟲PI4P5K參與在鐵刺激PIP2產生的功能角色, in 臺灣大學微生物研究所. 2018. 37. 諶彥汝, 不同陰道滴蟲分離株磷脂酰肌醇4-磷酸5-激酶表現量差異分析, in 臺灣大學微生物研究所. 2021. 38. Hongu, T. and Y. Kanaho, Activation machinery of the small GTPase Arf6. Adv Biol Regul, 2014. 54: p. 59-66. 39. D'Souza-Schorey, C. and P. Chavrier, ARF proteins: roles in membrane traffic and beyond. Nat Rev Mol Cell Biol, 2006. 7(5): p. 347-58. 40. D'Souza-Schorey, C., et al., A regulatory role for ARF6 in receptor-mediated endocytosis. Science, 1995. 267(5201): p. 1175-8. 41. Peters, P.J., et al., Overexpression of wild-type and mutant ARF1 and ARF6: distinct perturbations of nonoverlapping membrane compartments. J Cell Biol, 1995. 128(6): p. 1003-17. 42. Brown, F.D., et al., Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic. J Cell Biol, 2001. 154(5): p. 1007-17. 43. Altschuler, Y., et al., ADP-ribosylation factor 6 and endocytosis at the apical surface of Madin-Darby canine kidney cells. J Cell Biol, 1999. 147(1): p. 7-12. 44. Claing, A., et al., beta-Arrestin-mediated ADP-ribosylation factor 6 activation and beta 2-adrenergic receptor endocytosis. J Biol Chem, 2001. 276(45): p. 42509-13. 45. Aikawa, Y. and T.F. Martin, ARF6 regulates a plasma membrane pool of phosphatidylinositol(4,5)bisphosphate required for regulated exocytosis. J Cell Biol, 2003. 162(4): p. 647-59. 46. Donaldson, J.G. and C.L. Jackson, ARF family G proteins and their regulators: roles in membrane transport, development and disease. Nat Rev Mol Cell Biol, 2011. 12(6): p. 362-75. 47. Volpicelli-Daley, L.A., et al., Isoform-selective effects of the depletion of ADP-ribosylation factors 1-5 on membrane traffic. Mol Biol Cell, 2005. 16(10): p. 4495-508. 48. Honda, A., et al., Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation. Cell, 1999. 99(5): p. 521-32. 49. Radhakrishna, H., R.D. Klausner, and J.G. Donaldson, Aluminum fluoride stimulates surface protrusions in cells overexpressing the ARF6 GTPase. J Cell Biol, 1996. 134(4): p. 935-47. 50. Donaldson, J.G., Multiple roles for Arf6: sorting, structuring, and signaling at the plasma membrane. J Biol Chem, 2003. 278(43): p. 41573-6. 51. Winterbourn, C.C., Toxicity of iron and hydrogen peroxide: the Fenton reaction. Toxicol Lett, 1995. 82-83: p. 969-74. 52. Lill, R., Function and biogenesis of iron-sulphur proteins. Nature, 2009. 460(7257): p. 831-8. 53. Sanvisens, N., et al., Regulation of ribonucleotide reductase in response to iron deficiency. Mol Cell, 2011. 44(5): p. 759-69. 54. Abbaspour, N., R. Hurrell, and R. Kelishadi, Review on iron and its importance for human health. J Res Med Sci, 2014. 19(2): p. 164-74. 55. Figueroa-Angulo, E.E., et al., RNA-Binding Proteins in Trichomonas vaginalis: Atypical Multifunctional Proteins. Biomolecules, 2015. 5(4): p. 3354-95. 56. Kim, I., E.A. Yetley, and M.S. Calvo, Variations in iron-status measures during the menstrual cycle. Am J Clin Nutr, 1993. 58(5): p. 705-9. 57. Lehker, M.W., R. Arroyo, and J.F. Alderete, The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis. J Exp Med, 1991. 174(2): p. 311-8. 58. Garcia, A.F., et al., Iron and contact with host cells induce expression of adhesins on surface of Trichomonas vaginalis. Mol Microbiol, 2003. 47(5): p. 1207-24. 59. Rendon-Gandarilla, F.J., et al., The TvLEGU-1, a legumain-like cysteine proteinase, plays a key role in Trichomonas vaginalis cytoadherence. Biomed Res Int, 2013. 2013: p. 561979. 60. Solano-Gonzalez, E., et al., The trichomonad cysteine proteinase TVCP4 transcript contains an iron-responsive element. FEBS Lett, 2007. 581(16): p. 2919-28. 61. Hernandez-Gutierrez, R., J. Ortega-Lopez, and R. Arroyo, A 39-kDa cysteine proteinase CP39 from Trichomonas vaginalis, which is negatively affected by iron may be involved in trichomonal cytotoxicity. J Eukaryot Microbiol, 2003. 50 Suppl: p. 696-8. 62. Alvarez-Sanchez, M.E., et al., Negative iron regulation of the CP65 cysteine proteinase cytotoxicity in Trichomonas vaginalis. Microbes Infect, 2007. 9(14-15): p. 1597-605. 63. Hsu, H.M., et al., Signal transduction triggered by iron to induce the nuclear importation of a Myb3 transcription factor in the parasitic protozoan Trichomonas vaginalis. J Biol Chem, 2014. 289(42): p. 29334-49. 64. Dias-Lopes, G., et al., Morphologic study of the effect of iron on pseudocyst formation in Trichomonas vaginalis and its interaction with human epithelial cells. Mem Inst Oswaldo Cruz, 2017. 112(10): p. 664-673. 65. Helms, J.B. and J.E. Rothman, Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF. Nature, 1992. 360(6402): p. 352-4. 66. Renault, L., B. Guibert, and J. Cherfils, Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor. Nature, 2003. 426(6966): p. 525-30. 67. Zeeh, J.C., et al., Dual specificity of the interfacial inhibitor brefeldin a for arf proteins and sec7 domains. J Biol Chem, 2006. 281(17): p. 11805-14. 68. Cherfils, J. and P. Melancon, On the action of Brefeldin A on Sec7-stimulated membrane-recruitment and GDP/GTP exchange of Arf proteins. Biochem Soc Trans, 2005. 33(Pt 4): p. 635-8. 69. Peyroche, A., et al., Brefeldin A acts to stabilize an abortive ARF-GDP-Sec7 domain protein complex: involvement of specific residues of the Sec7 domain. Mol Cell, 1999. 3(3): p. 275-85. 70. Repella, T.L., et al., Arf6-dependent intracellular trafficking of Pasteurella multocida toxin and pH-dependent translocation from late endosomes. Toxins (Basel), 2011. 3(3): p. 218-41. 71. Zeghouf, M., et al., Arf, Sec7 and Brefeldin A: a model towards the therapeutic inhibition of guanine nucleotide-exchange factors. Biochem Soc Trans, 2005. 33(Pt 6): p. 1265-8. 72. Ryu, J.S., et al., Effect of iron on the virulence of Trichomonas vaginalis. J Parasitol, 2001. 87(2): p. 457-60. 73. M.D. Abramoff, P.J.M., S.J. Ram, Image Processing with ImageJ. Biophotonics International, 2004. 11(7): p. 36-42. 74. Wang, K.H., et al., An Atypical F-Actin Capping Protein Modulates Cytoskeleton Behaviors Crucial for Trichomonas vaginalis Colonization. Microbiol Spectr, 2023. 11(4): p. e0059623. 75. Pasqualato, S., et al., The structural GDP/GTP cycle of human Arf6. EMBO Rep, 2001. 2(3): p. 234-8. 76. Neuwald, A.F., Bayesian classification of residues associated with protein functional divergence: Arf and Arf-like GTPases. Biol Direct, 2010. 5: p. 66. 77. Smulders, L., et al., Phosphatidylinositol Monophosphates Regulate the Membrane Localization of HSPA1A, a Stress-Inducible 70-kDa Heat Shock Protein. Biomolecules, 2022. 12(6). 78. Park, S.J., T. Itoh, and T. Takenawa, Phosphatidylinositol 4-phosphate 5-kinase type I is regulated through phosphorylation response by extracellular stimuli. J Biol Chem, 2001. 276(7): p. 4781-7. 79. Beaudoin, J., et al., Fission yeast RNA-binding proteins Puf2 and Puf4 are involved in repression of ferrireductase Frp1 expression in response to iron. Mol Microbiol, 2021. 116(5): p. 1361-1377. 80. Satoh, R., et al., Fission Yeast PUF Proteins Puf3 and Puf4 Are Novel Regulators of PI4P5K Signaling. Biol Pharm Bull, 2023. 46(2): p. 163-169. 81. Meng, X., et al., Triggered Golgi membrane enrichment promotes PtdIns(4,5)P2 generation for plasma membrane repair. J Cell Biol, 2023. 222(8). 82. Oh, B.C., Phosphoinositides and intracellular calcium signaling: novel insights into phosphoinositides and calcium coupling as negative regulators of cellular signaling. Exp Mol Med, 2023. 55(8): p. 1702-1712. 83. Ziomek, G., C. van Breemen, and M. Esfandiarei, Drop in endo/sarcoplasmic calcium precedes the unfolded protein response in Brefeldin A-treated vascular smooth muscle cells. Eur J Pharmacol, 2015. 764: p. 328-339. 84. Vitale, N., et al., Calcium-regulated exocytosis of dense-core vesicles requires the activation of ADP-ribosylation factor (ARF)6 by ARF nucleotide binding site opener at the plasma membrane. J Cell Biol, 2002. 159(1): p. 79-89. 85. Chan, W.W.R., et al., ARF6-Rac1 signaling-mediated neurite outgrowth is potentiated by the neuronal adaptor FE65 through orchestrating ARF6 and ELMO1. FASEB J, 2020. 34(12): p. 16397-16413. 86. Schafer, D.A., C. D'Souza-Schorey, and J.A. Cooper, Actin assembly at membranes controlled by ARF6. Traffic, 2000. 1(11): p. 892-903. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/102077 | - |
| dc.description.abstract | 高等真核細胞ADP ribosylation factor (ARF) 結合三磷酸鳥苷 (Guanosine triphosphate) 成為活化態，是真核細胞中細胞膜運輸與細胞骨架之關鍵調節因子，其功能之一可活化磷脂醯肌醇-4-磷酸5-激酶 (phosphatidylinositol 4-phosphate 5-kinase, PI4P5K) 並與PI4P5K形成複合體共同移動至細胞膜以產生Phosphatidylinositol 4,5-bisphosphate (PIP2)。在陰道滴蟲細胞內，前人發現鐵離子刺激後能活化ARF6同源蛋白(TvARF220)，與TvPI4P5K形成複合體促進PIP2 產生，並調控下游鈣離子訊號。本研究目標在探討鐵如何在陰道滴蟲透過活化TvARF220來調控PIP2訊息傳導及其下游細胞功能。利用免疫螢光分析發現，TvPI4P5K與PIP2在陰道滴蟲細胞中共同定位在細胞膜上，並與TvARF220細胞內分布成正相關。同時，鐵刺激會增加TvPI4P5K與TvARF220共同轉移到細胞膜上，趨勢與鐵刺激後細胞膜上PIP2含量增加一致。相反地，當利用布雷非德菌素A (brefeldin A, BFA) 抑制陰道滴蟲TvARF220活化，TvARF220與TvPI4P5K共同轉移至細胞膜周邊的趨勢則下降，同時造成細胞膜上PIP2訊號降低。這些發現表明TvARF220功能可促進TvPI4P5K轉移到細胞膜上並增加PIP2產生。值得注意的是，鈣離子螢光指示劑顯示BFA處理造成陰道滴蟲內鈣訊號量降低的同時，也減緩肌動蛋白細胞骨架重組幅度，並造成細胞骨架主導之阿米巴型態遷移能力下降，從而確認TvARF220可作為PIP2與鈣離子等訊號的上游因子，最終調控肌動蛋白相關作用。本研究確認TvARF220作為引導特定蛋白細胞膜定位的關鍵功能，為陰道滴蟲寄生與宿主交互作用時的分子機制提供新見解。 | zh_TW |
| dc.description.abstract | Small GTPases of the ADP-ribosylation factor (ARF) family are key regulators of membrane trafficking and cytoskeletal dynamics in eukaryotic cells. In the protozoan parasite Trichomonas vaginalis, iron was previously found to activate an ARF6 homolog (TvARF220) and trigger its complex formation with TvPI4P5K, regulating downstream calcium signaling. This study aims to investigate the mechanism of how iron induces PIP2 signaling and its downstream cellular functions through TvARF220 activation in this parasite. Immunofluorescence analysis revealed that the plasma membrane colocalization of TvPI4P5K and its lipid product PIP2 strongly correlated with TvARF220 cellular distribution. Iron challenge enhanced TvPIP4P5K and TvARF220 enrichment at the plasma membrane, coinciding with increased PIP2 levels. In contrast, ARF inhibitor brefeldin A (BFA) diminished the plasma membrane localization of TvPI4P5K and TvARF220, resulting in reduced PIP2 production. These findings suggest that TvARF220 facilitates TvPI4P5K trafficking to the plasma membrane, thereby promoting localized PIP2 synthesis. Notably, BFA treatment also led to decreased intracellular calcium levels, impaired actin dynamics, and slowed down actin cytoskeleton-dependent amoeboid motility, supporting that TvARF220 acts upstream of a PIP2-calcium axis critical for actin-dependent processes. Together, this study highlights TvARF220 as a key regulator of membrane-localization signaling, offering new insights into the molecular basis of parasite colonization and host interaction. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2026-03-13T16:13:58Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2026-03-13T16:13:58Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書…………………………………………………………………… i
誌謝…………………………………………………………………………………... ii 中文摘要…………………………………………………………………………….. iii 英文摘要…………………………………………………………………………….. iv 第一章、 前言………………………………………………………………………. 1 第一節、 陰道滴蟲簡介…………………………………………………………. 1 第二節、 陰道滴蟲致病機轉……………………………………………………. 2 第三節、 陰道滴蟲型態……………………………………………………….… 2 第四節、 磷脂醯肌醇4,5-雙磷酸 ( Phosphatidylinositol 4,5-bisphosphate, PIP2 )………………………………………………………………………………. 3 第五節、 ADP-ribosylation factor ( ARF, ADP核糖基化因子 )……………... 4 第六節、 陰道滴蟲與鐵離子………………………………………………….… 5 第七節、 TvARF220與BFA……………………………………………………. 5 第八節、 實驗目的………………………………………………………………. 6 第二章、 材料與方法………………………………………………………………. 7 第一節、 陰道滴蟲蟲株……………………………………………………….… 7 1.1 陰道滴蟲種類……………………………………………………………… 7 1.2 陰道滴蟲培養……………………………………………………………… 7 1.3 備用蟲株存儲與再活化…………………………………………………… 7 1.4 台盼藍生存率測試………………………………………………………… 7 1.5 BFA處理條件………………………………………………………….…. 8 第二節、 免疫螢光染色 ( Immunofluorescence assay，IFA )………………… 8 2.1 活細胞自然貼附…………………………………………………………… 8 2.2 PIP2染色前處理………………………………………………………….. 8 2.3 抗體作用與封片……………………………………………………….…... 9 第三節、 Calcium green螢光染色……………………………………………… 9 第四節、 蛋白質聚丙烯醯胺凝膠電泳 (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis)…………………………………………... 10 第五節、 西方墨點法 ( Western blot assay )…………………………………... 10 第六節、G-Actin/F-actin In Vivo Assay, 肌動蛋白沉降分萃………………... 10 第七節、 細胞遷移癒合試驗 ( Wound-healing cell migration assay )……….... 11 第三章、 結果……………………………………………………………………… 12 第一節、 人類ARF6與陰道滴蟲ARF220序列相似度…………………..…. 12 第二節、 TvARF220, TvPI4P5K與PIP2於陰道滴蟲的分布情形………...… 12 第三節、 BFA處理之陰道滴蟲生存率比較……………………………….….. 12 第四節、 BFA對TvARF220, TvPI4P5K與PIP2在陰道滴蟲的細胞膜分布. 13 第五節、 BFA影響鐵調控TvPI4P5K在陰道滴蟲的表現與細胞膜分布…... 13 第六節、 BFA影響陰道滴蟲PIP2生成量……………………………………. 14 第七節、 BFA處理對陰道滴蟲鈣離子的影響………………………….….…. 14 第八節、 BFA處理對陰道滴蟲actin組裝的影響………………………….… 14 第九節、 BFA處理對陰道滴蟲遷移能力的影響……………………………… 15 總結 ………………………………………………………………..….........… 15 第四章、 討論……………………………………………………………………… 16 第一節、 人類ARF6與TvARF220序列相似………………………….…….. 16 第二節、 PIP2訊號傳遞因子間的關聯性……………………………….…….. 16 第三節、 鐵離子的刺激對PIP2訊息傳遞之影響……………………….…….. 17 第四節、 TvARF220在PIP2訊號傳遞中扮演的角色………………………… 17 第五節、 TvARF220在PIP2訊號傳遞中對下游鈣離子之影響……………… 18 第六節、 BFA對陰道滴蟲細胞爬行能力與actin組裝的影響………………. 18 參考文獻…………………………………………………………………….……… 48 附錄…………………………………………………………………………….…… 55 附錄一、TYI-S33培養液……………………………………………………. 55 附錄二、綜合抑制劑與必要成分………………………………………….… 56 附錄三、抗體……………………………………………………………….… 57 附錄四、西方墨點法使用溶液…………………………………………….… 58 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 陰道滴蟲 | - |
| dc.subject | 磷脂醯肌醇4,5-雙磷酸 | - |
| dc.subject | 磷脂醯肌醇-4-磷酸5-激酶 | - |
| dc.subject | ADP核糖基化因子 | - |
| dc.subject | 鐵離子 | - |
| dc.subject | 鈣離子 | - |
| dc.subject | 肌動蛋白 | - |
| dc.subject | Trichomonas vaginalis | - |
| dc.subject | phosphatidylinositol 4,5-bisphosphate | - |
| dc.subject | phosphatidylinositol-4-phosphate 5-kinase | - |
| dc.subject | ADP-ribosylation factor | - |
| dc.subject | Iron | - |
| dc.subject | Calcium | - |
| dc.subject | Actin | - |
| dc.title | 陰道滴蟲ARF蛋白調控PIP2訊息之功能探討 | zh_TW |
| dc.title | The functional study of the ARF protein in regulating PIP2 signaling of Trichomonas vaginalis | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 114-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 孫錦虹;許書豪 | zh_TW |
| dc.contributor.oralexamcommittee | Chin-Hung Sun;Shu-Hao Hsu | en |
| dc.subject.keyword | 陰道滴蟲,磷脂醯肌醇4,5-雙磷酸磷脂醯肌醇-4-磷酸5-激酶ADP核糖基化因子鐵離子鈣離子肌動蛋白 | zh_TW |
| dc.subject.keyword | Trichomonas vaginalis,phosphatidylinositol 4,5-bisphosphatephosphatidylinositol-4-phosphate 5-kinaseADP-ribosylation factorIronCalciumActin | en |
| dc.relation.page | 58 | - |
| dc.identifier.doi | 10.6342/NTU202504765 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2025-12-05 | - |
| dc.contributor.author-college | 醫學院 | - |
| dc.contributor.author-dept | 微生物學研究所 | - |
| dc.date.embargo-lift | 2026-03-14 | - |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-114-1.pdf | 4.92 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。