類別:http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/1222024-03-29T06:24:40Z2024-03-29T06:24:40Z齒顎矯正治療成效評估之探討-以臺灣專科考試為例Yi-Jun Hung洪翊珺http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/152262021-06-07T17:28:42Z2019-01-01T00:00:00Z標題: 齒顎矯正治療成效評估之探討-以臺灣專科考試為例; Assessing orthodontic treatment outcomes: analysis with the Taiwan Board of Orthodontics objective grading system
作者: Yi-Jun Hung; 洪翊珺
摘要: 摘要
目的
齒顎矯正醫師以改善病人齒列之美觀以及為病人建立理想咬合做為治療的目標,而許多因素會影響到矯正治療之成效,包含了病人初始牙齒狀況、病患配合程度、治療方式及不同的治療目標等。而監測矯正治療後成效對於治療品質的控制非常重要,鑑別出問題所在對於制定改善臨床結果的策略至關重要。除了需要暸解理想咬合之定義外,更需要有一套以合理且客觀的評分制度做為治療後成效的依據來評斷治療成效的好壞。
本研究之實驗目的為藉由「中華民國齒顎矯正部定專科醫師口試病例審查評分標準」(Taiwan Board of Orthodontics Grading System, TBO-OGS)來評估矯正治療的成效,並分析此系統之評分信度。
研究程序及方法
從臺大醫院齒顎矯正科治療完成的病人中挑選三十六例,條件符合中華民國齒顎矯正學會專科醫師考試病例屬性且病歷資料完整。挑選九位一般醫師(General dentist)、九位受訓醫師(Graduate student)、以及九位專科醫師(Orthodontist),總共二十七位醫師擔任本次研究之評分者,並請評分醫師依照TBO-OGS做兩次評分,時間間隔兩週。統計計算前後測差異分析及再測信度分析、整體評分者間信度、總分與不同資歷背景醫師之迴歸分析、各評分項目與不同資歷背景醫師之迴歸分析以及九種病例屬性評分間總分信度分析。
結果
本研究結果發現在不同資歷背景的醫師或不同屬性分類下,其評分前後測總分經paired t檢定皆未達統計上顯著差異。利用Pearson correlation進行分析,整體再測信度為高度相關(r=0.814),表示其前後測一致性較高;以醫師資歷背景做分類去探討,三種資歷背景醫師的再測信度皆為高度相關。
針對各評分項目之整體評分者間信度,各評分者間一致性較高之評分項目:垂直覆蓋;一致性中高之評分項目:鄰接面接觸、牙根平行度、中線、牙根吸收;一致性尚可之評分項目:水平覆蓋、臼齒關係、咬合接觸;一致性較低之評分項目:犬齒關係、邊緣脊、頰舌向牙齒傾斜、排列/旋轉。
不同資歷背景醫師評分總分之分析,評分之總分不受評分者之資歷背景而有所影響。針對各項評分項目評分與不同資歷背景醫師之回歸分析,評分項目中僅在牙根吸收此項目受訓醫師相較於一般醫師給較高的分數機會是0.34倍,牙根吸收此項目專科醫師相較於受訓醫師給較高的分數機會是2.94倍。針對九種屬性總分之評分者間信度為尚可至中等(0.22-0.48)。
結論
1. 整體而言,評分前後測之總分沒有差異,且前後測的分數相關性高。若以不同資歷背景來看,一般醫師、受訓醫師及專科醫師前後測數值皆為高度相關。若以不同屬性分類來看,Class I bimaxillary protrusion、Class II high angle、Class II div.2及anterior crossbite屬性前後測數值為中度相關性,其餘皆為高度相關。
2. 針對各個評分項目評分者間信度,高度一致性的為垂直覆蓋;中高度一致性的為鄰接面接觸、中線、牙根平行度及牙根吸收;一致性尚可的為水平覆蓋、臼齒關係及咬合接觸;一致性低的為犬齒關係、排列/旋轉、邊緣脊及頰舌向牙齒傾斜。
3. 針對不同資歷背景醫師評分總分之分析,評分之總分不受評分者之資歷背影而有所影響。
4. 對於大部分的評分項目,不同資歷背景醫師的各項評分沒有差異。而在牙根吸收項目,受訓醫師給的分數比一般醫師及專科醫師來得低。
5. 針對九種不同屬性類別的病例,其總分之評分者間信度為尚可至中等(0.22-0.48)。; Abstract
Objective
The orthodontists aim to improve the aesthetics of the patient's dentition and establish an ideal occlusion for the patient. Many factors including the initial dental condition of the patient, the degree of patient cooperation, the different treatment methods, and the different treatment goals etc., would affect the effectiveness of the treatment outcome. Monitoring treatment progress, final outcome and finding the problems for an orthodontic program are important for assuring quality control. In addition to understanding the definition of ideal occlusion, it is necessary to have a reasonable and objective scoring system as a basis for judging the effectiveness of treatment.
In this study, we evaluated the treatment outcome of 36 cases, analyzed the results of the raters with different training background, and assessed the reliability of the Taiwan Board of Orthodontics Grading System.
Material and methods
Nine general dentists, nine graduate students under orthodontic training, and nine certificated orthodontists, total twenty-seven raters, were recruited into the study. We randomly selected 36 patients who completed orthodontic treatment in National Taiwan University Hospital. The case should meet the case assessment form for the board exam of the Taiwan Board of Orthodontics. Paired t test and Pearson correlation, repeated linear regression model, Gwet’s AC2, repeated ordinal logistic regression, and mixed model calculate ICC (intra-class correlation) were used to analyze the data.
Results
There is no significant difference between pre-test and post-test, and data of pre-test and post-test have high correlation(r=0.814). That reveals that the interrater reliability is good. Categorized by different training background, there is no significant difference in the pre-test and post-test data which have high correlation. Categorized by different grading items, the analysis report reveals that raters reach the almost perfect strength of agreement in overbite item; substantial strength of agreement in interproximal contacts, root parallelism, midline, and root resorption items; fair strength of agreement in overjet, molar relationship, and occlusal contacts items; slight strength of agreement in canine relationship, marginal ridge, buccolingual inclination, and the alignment/rotation items.
There is no significant difference on the total scores by raters with different training background. Based on the outcome of repeated ordinal logistic regression, the graduate students would give the higher scores of root resorption item is 0.34 times than general dentists. The orthodontists would give the higher scores of root resorption item is 2.94 times than graduate students. Based to the nine different categories, the inter-rater reliability is fair to moderate (0.22-0.48).
Conclusion
1. There is no significant difference between pre-test and post-test, and data of pre-test and post-test have high correlation.
2. Based on the different items, the overbite, inter proximal contact, root parallelism and the root resorption have the better inter-rater reliability.
3. The training background of the examiners does not affect the total scores.
4. Based to the different categories, the inter-rater reliability is fair to moderate (0.22-0.48).
5. Intra-rater & inter-rater reliability of TBO-OGS is good.2019-01-01T00:00:00Z齒槽骨維持術的臨床表現統合分析Wei Chiang蔣蔚http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/565112021-06-16T05:32:17Z2014-01-01T00:00:00Z標題: 齒槽骨維持術的臨床表現統合分析; Meta-analysis of clinical performance of Alveolar ridge preservation
作者: Wei Chiang; 蔣蔚
摘要: 臨床上我們很常會面對到拔牙後齒槽骨縮減狀況。所以我們希望能夠集結許多臨床試驗,利用統合分析(meta-analysis),找出科學證據來:(1)評估齒槽骨維持術與拔牙後自然癒合的預期結果差別(2)比較各式齒槽骨維持術方法的同異與效益和(3)評估施行齒槽骨維持術後對於將植體植入理想位置的價值。做出一個統整。希望能幫助臨床醫師訂定及執行後續的治療計畫。
我們利用電子資料庫MEDLINE,Scopus Elsevier,Cochrane Library,全國期刊聯合資料庫,中國知識資源總庫、全國博碩士論文資訊加值網、華藝線上圖書館,及Google學術搜尋,全面系統性的搜尋了從西元1990到2014年4月與齒槽骨維持術相關的隨機對照試驗(Randomized controlled trial),橫段式研究(cross-sectional study),病例-對照研究(case-control study),世代追蹤研究法(cohort study),及有治療組、對照組,兩組以上互相比較的病例系列報告(case series)。然後用統合分析方式比較齒槽骨維持術使用不同材料及方法其臨床表現的優劣。
我們最後篩選出18篇文章。統合分析結果顯示,有做齒槽骨保存術的組別比未做齒槽骨保存術的組別在拔牙後有更少的骨嵴高度減少(加權平均差異,WMD = 1.074±0.291 mm(p<0.05))。更少的骨嵴寬度減少(加權平均差異,WMD = 1.981±0.24 mm(p<0.05))。用再生膜(Membrane) (p<0.001),異體骨(Xenograft) (p<0.001),以及島瓣一級縫合(primary closure) (p<0.001)等皆可降低拔牙後骨嵴高度減少。明顯有利於齒槽骨保存。至於在齒槽骨維持術治療對植牙治療長期成果的好處,並沒有足夠資料可用來得出結論。 本研究論證了齒槽骨維持術治療的重要性。但無法明確提供齒槽骨維持術使用的生物材料的類型,或手術過程清晰的指引。; Objective: This systematic review aims to the effect of socket preservation following tooth extraction in humans as compared with natural healing ,to evaluate the scientific evidence on the efficacy in the surgical protocols designed for preserving the alveolar ridge after tooth extraction and to evaluate how these techniques affect the placement of dental implants and the final implant supported restoration.
Material and methods: A thorough search in MEDLINE via Pubmed, Scopus Elsevier , MEDLINE (OvidSP), Cochrane Library, STICNET in Taiwan, NDLTD in Taiwan, Airiti library in Taiwan, CNKI in China and hand-searched the relevant journals from 1990 to April 2014. Randomized controlled trial, cross-sectional study. case-control study ,cohort study with a follow-up of at least 4 months reporting changes on both the hard and soft tissues (height and/or width) of the alveolar process (mm or %) after tooth extraction were considered for inclusion.
Results: The screening of titles and abstracts resulted in 18 publications meeting the eligibility criteria. Results from the meta-analyses showed a statistically significant greater ridge reduction in bone height & width for control groups as compared to test groups
Meta-regression analyses was based on the surgical protocol used for the ridge preservation (Membrane(Yes vs. No); Membrane (Yes(artifical) vs. No); Membrane (artificial vs autogenous); Xenograft (Yes vs. No); Graft (Yes vs. No); Flap (Yes vs. No); Primary Closure(Yes vs. No); Membrane(Yes)+Graft(Yes) vs. Membrane(Yes)+Graft (No)) and on the measurement method utilized to evaluate morphological changes. Meta-regression analyses demonstrated a statistically significant difference favoring the Membrane(Yes), Xenograft (Yes ) Primary Closure (Yes ) subgroup in terms of bone height & width .
Conclusions: The potential benefit of socket preservation therapies was demonstrated resulting in significantly less vertical and horizontal contraction of the alveolar bone crest. The scientific evidence does not provide clear guidelines in regards to the type of biomaterial, or surgical procedure, There are no data available to draw conclusions on the consequences of such benefits on the long-term outcomes of implant therapy.2014-01-01T00:00:00Z鼻竇補骨手術合併人工植牙治療後移植骨高度變化之回顧分析Hsiang-Yuan Cheng鄭翔元http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/238422021-06-08T05:11:38Z2011-01-01T00:00:00Z標題: 鼻竇補骨手術合併人工植牙治療後移植骨高度變化之回顧分析; Retrospective
Analysis
of
Graft
Height
Changes
after
Dental
Implantation
Combining
Maxillary
Sinus
Augmentation
作者: Hsiang-Yuan Cheng; 鄭翔元
摘要: 背景:
上顎後牙區缺牙患者常以植牙的方式重建口腔功能,但是由於上顎後牙區長期缺牙加上 鼻竇腔氣室化的緣故,導致骨量不足,常須合併做鼻竇補骨手術以增加骨量。手術主要是藉 由將鼻竇黏膜往上抬昇得到額外的空間,供人工或者自體移植骨植入,改善骨量不足,以利 進行人工植體之植入手術。文獻上有許多探討上顎後牙區骨量不足接受鼻竇補骨合併人工植 牙手術之成功率的相關研究,但是對於移植骨之長期穩定性、以及其影響因素的研究則較少。
研究目的:
本研究希望透過回顧病患之定期追蹤資料來觀察移植骨長期之穩定性,並且藉此找出影 響穩定性之關鍵因素,以確定治療方法之臨床效益。
研究方法:
本研究收集自 2002 至 2008 年於台大醫院口腔顎面外科門診接受人工植體種植合併鼻竇 補骨手術的病患。病患在植體植入手術後回診追蹤至少兩年以上才列入本研究。共 47 人,60 個上顎竇接受鼻竇補骨手術,以多孔性雙相磷酸鈣(macroporous biphasic calcium phosphate, MBCP)與去礦化冷凍乾燥移植骨(deproteinized freeze dried bone allograft, DFDBA)為補骨 材料,總計有 102 支植體。記錄各項可能影響植體成功率、移植骨穩定性之臨床因子,並藉 由各種影像學分析測量原有鼻竇高度、移植補骨後鼻竇高度,藉此測量移植骨長期之變化, 分三個位置量測,分別為距植體近心 2 mm 處、植體中心處、距植體遠心 2 mm 處;利用回診 時之鼻竇補骨後高度與原有鼻竇高度之比值來分析移植補骨後鼻竇高度經過長時間後與原有 鼻竇高度之關係;並將移植骨與植體根尖部之關係分為三類,第一類是植體根尖部上方仍有 明顯的移植骨存在,植體根尖部低於周圍移植骨高度,第二類是植體根尖部與周圍移植骨高 度相同,第三類是植體根尖部高於周圍移植骨高度;可以用來表示移植骨在長期下來和植體 之間的關係,亦可用來表示移植骨之穩定性。此外,並以統計學分析各項臨床因子與植體成 功率以及移植骨穩定性之關係。
研究結果:
102 支植體,有 2 支植體失敗,植體成功率為 98.03%,並沒有任何臨床因子與植體成功率有統計上之相關。
在移植骨穩定性方面,所有移植體中,自植體植入至回診時之移植骨變化量(graft height changes)在距植體近心 2 mm 處平均為 1.55±2.29 mm(減少 20.02%);植體中心處平均為 1.08±1.50 mm(減少 11.09%);距植體遠心 2 mm 處平均為 1.42±2.27 mm(減少 14.67%)。 DFDBA移植體中,移植體變化量在距植體近心2 mm處平均為3.17±2.50 mm(減少40.25%); 植體中心處平均為 1.52±1.33 mm(減少 14.78%);距植體遠心 2 mm 處平均為 3.21±2.50 mm (減少 35.02%)。MBCP 移植體中,移植骨變化量在距植體近心 2 mm 處平均為 0.84±1.80 mm (減少 10.95%);植體中心處平均為 0.90±1.54 mm(減少 9.54%);距植體遠心 2 mm 處平均 為 0.65±1.67 mm(減少 5.95%)。
回診時之移植補骨後鼻竇高度與原有鼻竇高度之比值(follow-up grafted sinus height / original sinus height, GSH(f/u) ratio),所有移植體中,在距植體近心 2 mm 處為 1.87±4.58; 距植體遠心 2 mm 處為 3.51±3.77,且兩個位置之比值有達到統計上顯著差異,P<0.05。DFDBA 組中,在距植體近心 2 mm 處為 0.66±0.82;距植體遠心 2 mm 處為 3.23±3.14,且兩個位置 之比值有達到統計上顯著差異,P<0.05。MBCP 組中,在距植體近心 2 mm 處為 2.43±5.6; 距植體遠心 2 mm 處為 3.63±4.03,兩個位置之比值並沒有達到統計上顯著差異,P>0.05。
植體根尖部與移植骨之關係(implant apex graft type):所有移植體中,第一類有 73 支 植體(72.2%),第二類有 11 支植體(10.8%),第三類有 17 支植體(16.8%)。DFDBA 組中, 第一類有 15 支植體(50.00%),第二類有 2 支植體(6.67%),第三類有 13 支植體(43.33%)。 MBCP 組中,第一類有 58 支植體(81.69%),第二類有 9 支植體(12.68%),第三類有 4 支 植體(5.63%)。
統計分析與移植骨穩定性相關之臨床因子,結果發現移植骨材料、植體相關位置、術中 鼻竇黏膜穿孔等與移植骨穩定性有關。移植骨穩定性 MBCP 比 DFDBA 明顯較好(P<0.05); 移植骨長期變化量在距植體近心、遠心 2 mm 處明顯多於植體中心處(P<0.05);在術中有鼻 竇黏膜穿孔者明顯多於無鼻竇黏膜穿孔者(P<0.05)。
結論:
鼻竇增高手術目前為一種十分常見之上顎補骨手術,本研究回顧病患之定期追蹤資料來觀察移植骨長期之穩定性及其臨床影響因子,發現移植骨材料、植體相關位置、術中鼻竇黏 膜穿孔等都與移植骨穩定性有統計上的顯著相關。; Background:
Dental implant therapy has become a standard rehabilitation method for patients suffered from missing teeth in the posterior maxilla. Because of disuse atrophy and continuous pneumatization of the maxillary sinus, insufficient bone height for dental implantation is common in the posterior maxilla which necessitates sinus augmentation to increase the bone volume. In this surgical technique the Schneiderian membrane is elevated and then bone graft materials are placed into the newly created subantral space to increase bone volume for implant therapy. Previous studies showed that the survival rates for dental implants in combination with sinus augmentation are high. However, the long-term stability of sinus graft and factors influencing graft stability have seldom been examined.
Purpose:
By examination of the changes in sinus graft volume over a long period and analysis of the relations between graft alteration and various clinical parameters, the long-term efficacy of the sinus augmentation technique is determined.
Materials and Methods:
Patients who received dental implantation in combination with sinus augmentation at the Department of Oral Maxillofacial Surgery, National Taiwan University Hospital during 2002 to 2008 were included in the study. A total of 47 patients were followed for at least two years. Macroporous biphasic calcium phosphate (MBCP) or deproteinized freeze dried bone allograft (DFDBA) were used as graft materials in 60 maxillary sinus augmentation with total 102 implants. Clinical factors that may influence implant success and graft stability were recorded. Changes in graft height were calculated in three locations, 2 mm mesial to implant, directly above implant, and 2 mm distal to implant. The GSH ratio (follow-up grafted sius height versus original sius height) were calculated as follow-up grafted sinus height versus original sinus height. The relationship between grafted sinus floor and implant was classified into three groups: Group I, in which the grafted sinus floor was above the implant apex; Group II, in which the implant apex was level with the grafted sinus floor; and Group III, in which the grafted sinus floor was below the implant apex. The relations between various clinical parameters and implant success or graft stability were analyzed statistically by Chi Square Test, Student t Test, Logistic Regression Model.
Results:
There were 2 implants defined as failure, and the overall implant success rate was 98.03%. No clinical factor was significantly related to implant success, all P>0.05.
In average, the long term graft change at the site 2 mm mesial to implant was 1.55±2.29 mm (mean±SD)(loss 20.02%); directly above implant was 1.08±1.50 mm (loss 11.09%); 2 mm distal to implant was 1.42±2.27 mm (loss 14.67%). When DFDBA grafts were considered separately, the long term change at the site 2 mm mesial to implant was 3.17±2.50 mm (loss 40.25%); directly above implant was 1.52±1.33 mm (loss 14.78%); 2 mm distal to implant was 3.21±2.50 mm (loss 35.02%). In MBCP group, the long term change at the site 2 mm mesial to implant was 0.84±1.80 mm (loss 10.95%); directly above implant was 0.90±1.54 mm (loss 9.54%): 2 mm distal to implant was 0.65±1.67 mm (loss 5.95%).
For all sinus grafts, the mean follow-up GSH ratio 2 mm mesial to implant was 1.87±4.58; 2 mm distal to implant was 3.51±3.77. Loss of graft height at mesial site was smaller than that at the distal, and the difference was significant statistically (P<0.05). In DFDBA group, the follow-up GSH ratio at the site 2 mm mesial to implant was 0.66±0.82; 2 mm distal to implant was 3.23±3.14. And the difference was significant statistically (P<0.05). In MBCP group, follow-up GSH ratio at the site 2 mm mesial to implant was 2.43±5.6; 2 mm distal to implant was 3.63±4.03, but the difference was not significant statiscally (P>0.05).
The relations between grafted sinus floor and implant apex were as follows: 73 implants (72.2%) were in group I, 11 implants (10.8%) group II, 17 implants (16.8%) group III. In DFDBA group, 15 implants (50.00%) were in group I, 2 implants (6.67%) group II, 13 implants (43.33%) group III. In MBCP group, 58 implants (81.69%) were in group I, 9 implants (12.68%) group II, 4 implants (5.63%) group III.
Concerning the relations between graft stability and various clinical parameters were, we found that: graft materials, implant related location, intraoperative sinus membrane perforation and method of access window protection, were significantly associated with graft stability. The grafts were significantly more stable when MBCP was used comparing to DFDBA (P<0.05). Graft changes were significantly more obvious mesial to the implants comparing to the distal aspects (P<0.05). Graft loss was noted if membrane perforation happened during augmentation surgery (P<0.05). Graft changes were more marked when access windows were protected by collagen membrane comparing to window bone plate replacement (P<0.05).
Conclusions:
Sinus augmentation is now a routine bone graft technique for implant therapy. In the retrospective study, we noted that sinus grafts decfeased in size in the long term, although this phenomenon was not related to implant success. We also found that graft material, implant related location, intraoperative sinus membrane perforation were factors associated with long term graft stability.2011-01-01T00:00:00Z鹼性成纖維細胞生長因子對牙髓之增生、移動、分化及細胞間質生成的影響Ya-Chin Chang張雅芩http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/463362021-06-15T05:04:14Z2010-01-01T00:00:00Z標題: 鹼性成纖維細胞生長因子對牙髓之增生、移動、分化及細胞間質生成的影響; Effects of basic fibroblast growth factor on proliferation, migration, differentiation, and matrix production of human dental pulp cells
作者: Ya-Chin Chang; 張雅芩
摘要: 實驗目的:鹼性成纖維細胞生長因子(basic fibroblast growth factor, bFGF)功能包括促進傷口癒合、血管新生、黑色素形成、硬骨及軟骨的生成等…。本篇研究著重於鹼性成纖維細胞生長因子對於人類牙髓細胞在形態表現、細胞增生及生存能力、及細胞移動、細胞分化、細胞間質生成的影響,進而了解bFGF 在牙髓組織修復與重建所扮演的角色。
實驗方法:使用不同濃度的鹼性成纖維細胞生長因子(bFGF)刺激人類牙髓細胞。在不同的時間點下,以光學顯微鏡觀察細胞型態及移動情況。利用MTT 測定牙髓細胞的存活能力;以鹼性磷酸酶染色檢測細胞分化程度、以Sircol Collgen assay 做膠原蛋白定量。另外使用反轉錄鏈聚合反應(RT-PCR)印證相關基因的表現。
實驗結果:鹼性成纖維細胞生長因子(bFGF)會增加細胞數目;人類牙髓細胞在較高濃度的bFGF 刺激下,細胞的存活能力有兩倍至三倍的提升。而與細胞增生相關的基因(Cyclin B1, CDC2, CDC25c)表現會因應bFGF 濃度而增加。bFGF 亦促進牙髓細胞的傷口癒合及細胞移動速度。然而,Rho 和ROCK 基因的表現最高峰會落在bFGF 濃度為10 ng/ml 之上;a-sma 則因應bFGF 濃度而遞減。在細胞分化方面,bFGF 會降低鹼性磷酸酶的染色程度。在細胞間質生成方面,bFGF 對於膠原蛋白的含量沒有顯著影響;而相關基因(TIMP2, MMP2)則因應bFGF 刺激而下降。
結論:人類牙髓細胞受到短期bFGF 刺激後(五天內),細胞增生、移動、傷口癒合能力會因應生長因子而有所增進。然而,bFGF 會影響細胞分化及膠原蛋白的形成。; Aim: Basic fibroblast growth factor (bFGF) is multifunctional protein with a wide variety of effects. The functions of bFGF include wound healing, angiogenesis, melanogenesis, development of bone and cartilage, and so on. The purpose of our study is to investigate whether basic fibroblast growth factor influences the morphological changes, cell proliferation and viability, cell differentiation, and extracellular matrix formation of human dental pulp cells in vitro within the period of 5 days.
Materials and Methods: Primary-cultured human dental pulp cells were treated with different concentrations of bFGF (0, 1, 10, 50, 200 ng/ml). Morphology of pulp cells and cell migration were observed under light microscopy (40X). Cell proliferation was evaluated by MTT assay. Cell differentiation was evaluated by alkaline phosphatase (ALP) staining. Changes in mRNA expression of ALKP, Runx2, Cyclin B1, CDC2, CDC25c, p21, Rho, ROCK, a-sma, collagen type I, TIMP2, and MMP2 were determined by reverse-transcriptase polymerase chain reaction (RT-PCR). Collagen content was determined by Sircol Collagen assay.
Results: Dental pulp cells are spindle with extended cellular processes with/without bFGF treatment. The numbers of dental pulp cells without any bFGF treatment were much lower than those under the treatment of various concentrations of bFGF. Cell viability was two to three fold increased in higher concentrations of bFGF (50, 200 ng/ml). bFGF increased Cyclin B1, CDC2, and CDC25C mRNA gene expressions dose-dependently. Migrating rates were higher in the groups of higher concentrations of bFGF than those in the groups of lower concentrations. Rho and ROCK gene expressions were at peak at 10 ng/ml of bFGF. Nevertheless, α-sma mRNA gene expression of pulp cells declined in dose-dependent manner. bFGF downregulated ALPactivity of human dental pulp cells but did not affect significantly RUNX2 mRNA expression. bFGF did not affect significantly collagen content . Gene expression of MMP2 and TIMP2 were down-regulated by bFGF treatment.
Conclusion: Human dental pulp cells incubated with basic fibroblast growth factor demonstrated proliferative and migratory properties during the early stage of wound repairing (within 5 days). Meanwhile, bFGF had inverse effects on cell differentiation and extracellular matrix formation.2010-01-01T00:00:00Z