請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/99473完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳慧文 | zh_TW |
| dc.contributor.advisor | Hui-Wen Chen | en |
| dc.contributor.author | 彭輅 | zh_TW |
| dc.contributor.author | Lu Peng | en |
| dc.date.accessioned | 2025-09-10T16:23:48Z | - |
| dc.date.available | 2025-09-11 | - |
| dc.date.copyright | 2025-09-10 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-07-17 | - |
| dc.identifier.citation | 張秀梅, 2004. 台灣家禽腺病毒之基因與血清分型、病原性研究及血清抗體調查. 國立臺灣大學, 台北市.
蔡淳恩, 2018. 禽腺病毒第四血清型疫苗之研發. 國立屏東科技大學, 屏東縣. Anjum, A.D., Sabri, M.A., Iqbal, Z., 1989. Hydropericarditis syndrome in broiler chickens in Pakistan. Vet Rec 124, 247-248. Archetti, I., Horsfall, F.L., Jr., 1950. Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum. J Exp Med 92, 441-462. Benkő, M., Aoki, K., Arnberg, N., Davison, A.J., Echavarría, M., Hess, M., Jones, M.S., Kaján, G.L., Kajon, A.E., Mittal, S.K., Podgorski, I.I., San Martín, C., Wadell, G., Watanabe, H., Harrach, B., Consortium, I.R., 2022. ICTV Virus Taxonomy Profile: Adenoviridae 2022. Journal of General Virology 103. Bertran, K., Blanco, A., Antilles, N., Nofrarías, M., Valle, R.M., Cobos, À., Ramis, A., Biarnés, M., Majó, N., 2021. A 10-Year Retrospective Study of Inclusion Body Hepatitis in Meat-Type Chickens in Spain (2011-2021). Viruses 13. Brown Jordan, A., Blake, L., Bisnath, J., Ramgattie, C., Carrington, C.V., Oura, C.A.L., 2019. Identification of four serotypes of fowl adenovirus in clinically affected commercial poultry co-infected with chicken infectious anaemia virus in Trinidad and Tobago. Transbound Emerg Dis 66, 1341-1348. Butt, S.L., Taylor, T.L., Volkening, J.D., Dimitrov, K.M., Williams-Coplin, D., Lahmers, K.K., Miller, P.J., Rana, A.M., Suarez, D.L., Afonso, C.L., Stanton, J.B., 2018. Rapid virulence prediction and identification of Newcastle disease virus genotypes using third-generation sequencing. Virol J 15, 179. Carlson, H.C., al-Sheikhly, F., Pettit, J.R., Seawright, G.L., 1974. Virus particles in spleens and intestines of turkeys with hemorrhagic enteritis. Avian Dis 18, 67-73. Caserta, L.C., Zhang, J., Piñeyro, P., Diel, D.G., 2023. Rapid genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) using MinION nanopore sequencing. PLoS One 18, e0282767. Chapman, R., Jones, L., D'Angelo, A., Suliman, A., Anwar, M., Bagby, S., 2023. Nanopore-Based Metagenomic Sequencing in Respiratory Tract Infection: A Developing Diagnostic Platform. Lung 201, 171-179. Chavan, V.G., Awandkar, S.P., Kulkarni, R.C., Chavhan, S.G., Suryawanshi, R.D., Jadhav, R.K., Kulkarni, M.B., Agnihotri, A.A., 2024. Isolation and molecular characterization of fowl adenovirus from inclusion body hepatitis cases in Western India during 2019-2021. Iran J Vet Res 25, 62-68. Chen, J., Xu, F., 2023. Application of Nanopore Sequencing in the Diagnosis and Treatment of Pulmonary Infections. Mol Diagn Ther 27, 685-701. Chen, L., Yin, L., Peng, P., Zhou, Q., Du, Y., Zhang, Y., Xue, C., Cao, Y., 2020. Isolation and Characterization of A Novel Fowl Adenovirus Serotype 8a Strain from China. Virol Sin 35, 517-527. Chen, L., Yin, L., Zhou, Q., Peng, P., Du, Y., Liu, L., Zhang, Y., Xue, C., Cao, Y., 2019. Epidemiological investigation of fowl adenovirus infections in poultry in China during 2015-2018. BMC Vet Res 15, 271. Choi, K.S., Kye, S.J., Kim, J.Y., Jeon, W.J., Lee, E.K., Park, K.Y., Sung, H.W., 2012. Epidemiological investigation of outbreaks of fowl adenovirus infection in commercial chickens in Korea. Poult Sci 91, 2502-2506. Davison, A.J., Benkő, M., Harrach, B., 2003. Genetic content and evolution of adenoviruses. J Gen Virol 84, 2895-2908. De la Torre, D., Nuñez, L.F.N., Santander Parra, S.H., Astolfi-Ferreira, C.S., Piantino Ferreira, A.J., 2018. Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil. Virusdisease 29, 83-88. Fender, P., Boussaid, A., Mezin, P., Chroboczek, J., 2005. Synthesis, cellular localization, and quantification of penton-dodecahedron in serotype 3 adenovirus-infected cells. Virology 340, 167-173. Fitzgerald, S.D., Rautenschlein, S., Mahsoub, H.M., Pierson, F.W., Reed, W.M., Jack, S.W. 2020. Adenovirus Infections, In: Diseases of Poultry. 321-363. Helmboldt, C.F., Frazier, M.N., 1963. Avian hepatic inclusion bodies of unknown significance. Avian Dis 7, 446-450. Hess, M., Raue, R., Prusas, C., 1999. Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium. Avian Pathol 28, 433-439. Hosseini, H., Najafi, H., Fallah Mehrabadi, M.H., Gholamian, B., Noroozi, S., Ahmadi, M., Ziafati Kafi, Z., Sadri, N., Hojabr Rajeoni, A., Ghalyanchilangeroudi, A., 2021. Molecular detection of fowl adenovirus 7 from slaughtered broiler chickens in Iran: the first report. Iran J Vet Res 22, 244-247. Islam, M.N., Rahman, M.M., Rahman, M.K., Alam, J., 2023. First Evidence of Fowl Adenovirus Induced Inclusion Body Hepatitis in Chicken in Bangladesh. Can J Infect Dis Med Microbiol 2023, 7253433. Jain, M., Olsen, H.E., Paten, B., Akeson, M., 2016. The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community. Genome Biol 17, 239. Jia, Z., Pan, X., Zhi, W., Chen, H., Bai, B., Ma, C., Ma, D., 2022. Probiotics Surface-Delivering Fiber2 Protein of Fowl Adenovirus 4 Stimulate Protective Immunity Against Hepatitis-Hydropericardium Syndrome in Chickens. Front Immunol 13, 919100. Kaján, G.L., Kecskeméti, S., Harrach, B., Benkő, M., 2013. Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity. Vet Microbiol 167, 357-363. Kawaguchi, T., Nomura, K., Hirayama, Y., Kitagawa, T., 1987. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res 47, 4460-4464. Khawaja, D., Ahmad, S., Rauf, A., Zulfiqar, M., Mahmood, S., Mahmood-ul-Hasan, M.-u.-H., 1988. Isolation of an adeno virus from hydropericardium syndrome in broiler chicks. Kim, H.R., Song, H.S., Jang, I., Thai, T.N., Kim, H.S., Kwon, Y.K., Her, M., 2025. Nationwide Surveillance of Fowl Adenovirus Infection and Coinfection With Other Diseases on Slaughter Broiler in South Korea. Transbound Emerg Dis 2025, 9353432. Kim, M.S., Lim, T.H., Lee, D.H., Youn, H.N., Yuk, S.S., Kim, B.Y., Choi, S.W., Jung, C.H., Han, J.H., Song, C.S., 2014. An inactivated oil-emulsion fowl Adenovirus serotype 4 vaccine provides broad cross-protection against various serotypes of fowl Adenovirus. Vaccine 32, 3564-3568. Kumar, R., Chandra, R., Shukla, S.K., Agrawal, D.K., Kumar, M., 1997. Hydropericardium syndrome (HPS) in India: a preliminary study on the causative agent and control of the disease by inactivated autogenous vaccine. Trop Anim Health Prod 29, 158-164. Li, P.H., Zheng, P.P., Zhang, T.F., Wen, G.Y., Shao, H.B., Luo, Q.P., 2017. Fowl adenovirus serotype 4: Epidemiology, pathogenesis, diagnostic detection, and vaccine strategies. Poult Sci 96, 2630-2640. Li, Y.T., Chen, T.C., Lin, S.Y., Mase, M., Murakami, S., Horimoto, T., Chen, H.W., 2020. Emerging lethal infectious bronchitis coronavirus variants with multiorgan tropism. Transbound Emerg Dis 67, 884-893. Liu, A., Zhang, Y., Wang, J., Cui, H., Qi, X., Liu, C., Zhang, Y., Li, K., Gao, L., Wang, X., Gao, Y., Pan, Q., 2022. Complete Genome Analysis and Animal Model Development of Fowl Adenovirus 8b. Viruses 14. Liu, J., Shi, X., Lv, L., Wang, K., Yang, Z., Li, Y., Chen, H., 2021. Characterization of Co-infection With Fowl Adenovirus Serotype 4 and 8a. Front Microbiol 12, 771805. Liu, L., Gao, W., Chang, J., Liu, J., Huang, Z., Sun, W., Song, Y., Li, X., 2025. Co-Infection of Chicken Infectious Anemia Virus and Fowl Adenovirus Serotype E8b Increases Mortality in Chickens. Viruses 17. Lv, L., Lu, H., Wang, K., Shao, H., Mei, N., Ye, J.Q., Chen, H.J., 2021. Emerging of a novel natural recombinant fowl adenovirus in China. Transbound Emerg Dis 68, 283-288. MacKenzie, M., Argyropoulos, C., 2023. An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations. Micromachines (Basel) 14. Marek, A., Kaján, G.L., Kosiol, C., Benkő, M., Schachner, A., Hess, M., 2016. Genetic diversity of species Fowl aviadenovirus D and Fowl aviadenovirus E. J Gen Virol 97, 2323-2332. Mase, M., Iseki, H., Watanabe, S., 2021. Complete Genome Sequence of a Fowl Adenovirus D Strain Isolated from Chickens with Inclusion Body Hepatitis in Japan. Microbiol Resour Announc 10, e0094021. Mase, M., Mitake, H., Inoue, T., Imada, T., 2009. Identification of group I-III avian adenovirus by PCR coupled with direct sequencing of the hexon gene. J Vet Med Sci 71, 1239-1242. Mase, M., Nakamura, K., 2014. Phylogenetic analysis of fowl adenoviruses isolated from chickens with gizzard erosion in Japan. J Vet Med Sci 76, 1535-1538. Matos, M., Grafl, B., Liebhart, D., Hess, M., 2016. The outcome of experimentally induced inclusion body hepatitis (IBH) by fowl aviadenoviruses (FAdVs) is crucially influenced by the genetic background of the host. Vet Res 47, 69. McFerran, J.B., Connor, T.J., 1977. Further studies on the classification of fowl adenoviruses. Avian Dis 21, 585-595. McFerran, J.B., Smyth, J.A., 2000. Avian adenoviruses. Rev Sci Tech 19, 589-601. Meng, K., Yuan, X., Yu, J., Zhang, Y., Ai, W., Wang, Y., 2019. Identification, Pathogenicity of Novel Fowl Adenovirus Serotype 4 SDJN0105 in Shandong, China and Immunoprotective Evaluation of the Newly Developed Inactivated Oil-emulsion FAdV-4 Vaccine. Viruses 11. Mete, A., Armien, A.G., Rejmanek, D., Mott, M., Crossley, B.M., 2021. Emergence of fowl aviadenovirus C-4 in a backyard chicken flock in California. J Vet Diagn Invest 33, 806-809. Meulemans, G., Boschmans, M., Berg, T.P., Decaesstecker, M., 2001. Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathol 30, 655-660. Mikheyev, A.S., Tin, M.M., 2014. A first look at the Oxford Nanopore MinION sequencer. Mol Ecol Resour 14, 1097-1102. Mittal, D., Jindal, N., Tiwari, A.K., Khokhar, R.S., 2014. Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens. Virusdisease 25, 114-119. Mo, J., 2021. Historical Investigation of Fowl Adenovirus Outbreaks in South Korea from 2007 to 2021: A Comprehensive Review. Viruses 13. Niczyporuk, J.S., 2016. Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland. Arch Virol 161, 33-42. Niczyporuk, J.S., 2018. Deep analysis of Loop L1 HVRs1-4 region of the hexon gene of adenovirus field strains isolated in Poland. PLoS One 13, e0207668. Oliver-Ferrando, S., Dolz, R., Calderón, C., Valle, R., Rivas, R., Pérez, M., Biarnés, M., Blanco, A., Bertran, K., Ramis, A., Busquets, N., Majó, N., 2017. Epidemiological and pathological investigation of fowl aviadenovirus serotypes 8b and 11 isolated from chickens with inclusion body hepatitis in Spain (2011-2013). Avian Pathol 46, 157-165. Olson, N.O., 1951. A respiratory disease (bronchitis) of quail caused by a virus. Vet Med 46, 22. Pallister, J., Wright, P.J., Sheppard, M., 1996. A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses. J Virol 70, 5115-5122. Pan, Q., Liu, L., Gao, Y., Liu, C., Qi, X., Zhang, Y., Wang, Y., Li, K., Gao, L., Wang, X., Cui, H., 2017. Characterization of a hypervirulent fowl adenovirus 4 with the novel genotype newly prevalent in China and establishment of reproduction infection model of hydropericardium syndrome in chickens. Poult Sci 96, 1581-1588. Park, D.H., Lee, H.C., Youn, H.N., Ju, H.S., Kim, K.J., Go, S.H., Lee, D.Y., Lee, J.B., Lee, S.W., Song, C.S., 2021. Genetic Characterization and Pathogenicity Analysis of Recently Isolated Fowl Adenovirus 8b in Korea. Avian Dis 65, 122-131. Radwan, M.M., El-Deeb, A.H., Mousa, M.R., El-Sanousi, A.A., Shalaby, M.A., 2019. First report of fowl adenovirus 8a from commercial broiler chickens in Egypt: molecular characterization and pathogenicity. Poult Sci 98, 97-104. Roberts, M.M., White, J.L., Grütter, M.G., Burnett, R.M., 1986. Three-dimensional structure of the adenovirus major coat protein hexon. Science 232, 1148-1151. Rodr®™guez, J., Koga, Y., Alvarado, A., Tinoco, R., 2014. Molecular Characterization of Peruvian Fowl Adenovirus (FAdV) Isolates. Advances in Microbiology Vol.04No.10, 9. Ruan, S., Zhao, J., Yin, X., He, Z., Zhang, G., 2018. A subunit vaccine based on fiber-2 protein provides full protection against fowl adenovirus serotype 4 and induces quicker and stronger immune responses than an inactivated oil-emulsion vaccine. Infect Genet Evol 61, 145-150. Russell, W.C., 2009. Adenoviruses: update on structure and function. J Gen Virol 90, 1-20. Sabarudin, N.S., Tan, S.W., Phang, Y.F., Omar, A.R., 2021. Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific-pathogen-free chicken. J Vet Sci 22, e42. Schachner, A., Gonzalez, G., Endler, L., Ito, K., Hess, M., 2019. Fowl Adenovirus (FAdV) Recombination with Intertypic Crossovers in Genomes of FAdV-D and FAdV-E, Displaying Hybrid Serological Phenotypes. Viruses 11. Schachner, A., Grafl, B., Hess, M., 2021. Spotlight on avian pathology: fowl adenovirus (FAdV) in chickens and beyond - an unresolved host-pathogen interplay. Avian Pathol 50, 2-5. Schonewille, E., Singh, A., Göbel, T.W., Gerner, W., Saalmüller, A., Hess, M., 2008. Fowl adenovirus (FAdV) serotype 4 causes depletion of B and T cells in lymphoid organs in specific pathogen-free chickens following experimental infection. Vet Immunol Immunopathol 121, 130-139. Shah, M.S., Ashraf, A., Khan, M.I., Rahman, M., Habib, M., Chughtai, M.I., Qureshi, J.A., 2017. Fowl adenovirus: history, emergence, biology and development of a vaccine against hydropericardium syndrome. Arch Virol 162, 1833-1843. Sohaimi, N.M., Hair-Bejo, M., 2021. A recent perspective on fiber and hexon genes proteins analyses of fowl adenovirus toward virus infectivity-A review. Open Vet J 11, 569-580. Song, Y., Zhao, Z., Liu, L., Li, Y., Gao, W., Song, X., Li, X., 2023. Knob domain of Fiber 2 protein provides full protection against fowl adenovirus serotype 4. Virus Res 330, 199113. Spatz, S.J., Garcia, M., Riblet, S., Ross, T.A., Volkening, J.D., Taylor, T.L., Kim, T., Afonso, C.L., 2019. MinION sequencing to genotype US strains of infectious laryngotracheitis virus. Avian Pathol 48, 255-269. Su, Q., Liu, X., Li, Y., Meng, F., Cui, Z., Chang, S., Zhao, P., 2019. The intracorporal interaction of fowl adenovirus type 4 and LaSota strain significantly aggravates the pathogenicity of one another after using contaminated Newcastle disease virus-attenuated vaccine. Poult Sci 98, 613-620. Tamura, K., Stecher, G., Kumar, S., 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol 38, 3022-3027. Van Den Ende, M., Don, P.A., Kipps, A., 1949. The isolation in eggs of a new filtrable agent which may be the cause of bovine lumpy skin disease. J Gen Microbiol 3, 174-183. Van Eck, J.H., Davelaar, F.G., Heuvel-Plesman, T.A., Van Kol, N., Kouwenhoven, B., Guldie, F.H., 1976. Dropped egg production, soft shelled and shell-less eggs associated with appearance of precipitins to adenovirus in flocks of laying fowls. Avian Pathol 5, 261-272. Vera-Hernández, P.F., Morales-Garzón, A., Cortés-Espinosa, D.V., Galiote-Flores, A., García-Barrera, L.J., Rodríguez-Galindo, E.T., Toscano-Contreras, A., Lucio-Decanini, E., Absalón, A.E., 2016. Clinicopathological characterization and genomic sequence differences observed in a highly virulent fowl Aviadenovirus serotype 4. Avian Pathol 45, 73-81. Wang, T., Meng, F., Chen, C., Shen, Y., Li, P., Xu, J., Feng, Z., Qu, X., Wang, F., Li, B., Liu, M., 2023. Pathogenicity and epidemiological survey of fowl adenovirus in Shandong Province from 2021 to 2022. Front Microbiol 14, 1166078. Wang, X., Tang, Q., Chu, Z., Wang, P., Luo, C., Zhang, Y., Fang, X., Qiu, L., Dang, R., Yang, Z., 2018. Immune protection efficacy of FAdV-4 surface proteins fiber-1, fiber-2, hexon and penton base. Virus Res 245, 1-6. Xie, Z., Zhang, J., Sun, M., Zeng, Q., Huang, Y., Dong, J., Li, L., Huang, S., Liao, M., 2022. The first complete genome sequence and pathogenicity characterization of fowl adenovirus serotype 2 with inclusion body hepatitis and hydropericardium in China. Front Vet Sci 9, 951554. Xu, A.H., Sun, L., Tu, K.H., Teng, Q.Y., Xue, J., Zhang, G.Z., 2021. Experimental co-infection of variant infectious bursal disease virus and fowl adenovirus serotype 4 increases mortality and reduces immune response in chickens. Vet Res 52, 61. Yamaguchi, M., Miyaoka, Y., Hasan, M.A., Kabir, M.H., Shoham, D., Murakami, H., Takehara, K., 2022. Isolation and Molecular Characterization of Fowl Adenovirus and Avian Reovirus from Breeder Chickens in Japan in 2019-2021. J Vet Med Sci 84, 238-243. Yan, T., Zhu, S., Wang, H., Li, C., Diao, Y., Tang, Y., 2020. Synergistic pathogenicity in sequential coinfection with fowl adenovirus type 4 and avian orthoreovirus. Vet Microbiol 251, 108880. Zhang, L.L., Zhang, C., Peng, J.P., 2022. Application of Nanopore Sequencing Technology in the Clinical Diagnosis of Infectious Diseases. Biomed Environ Sci 35, 381-392. Zhang, Y., Xu, H., Tian, Y., Tang, J., Lin, H., Sun, Y., Zhao, Q., Zhou, E.M., Chen, Y., Liu, B., 2025. Coinfection of avian hepatitis E virus and different serotypes of fowl adenovirus in chicken flocks in Shaanxi, China. Microbiol Spectr 13, e0133824. Zhao, J., Zhong, Q., Zhao, Y., Hu, Y.X., Zhang, G.Z., 2015. Pathogenicity and Complete Genome Characterization of Fowl Adenoviruses Isolated from Chickens Associated with Inclusion Body Hepatitis and Hydropericardium Syndrome in China. PLoS One 10, e0133073. Zhuang, Q., Wang, S., Zhang, F., Zhao, C., Chen, Q., Zhao, R., Guo, P., Ju, L., Li, J., Hou, G., Chen, X., Sun, F., Wang, K., 2023. Molecular epidemiology analysis of fowl adenovirus detected from apparently healthy birds in eastern China. BMC Vet Res 19, 5. Zou, X., Rong, Y., Guo, X., Hou, W., Yan, B., Hung, T., Lu, Z., 2021. Fiber1, but not fiber2, is the essential fiber gene for fowl adenovirus 4 (FAdV-4). J Gen Virol 102. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/99473 | - |
| dc.description.abstract | 家禽腺病毒（Fowl adenoviruses, FAdVs）是引起雞隻包涵體性肝炎與肝心綜合症等疾病之重要病原體，對家禽產業造成重大經濟損失，尤其近年來台灣FAdV相關病例有上升趨勢，然而目前對於病毒株之各項特性分析卻相當有限。本研究針對2020至2025年間之疑似病例進行採樣與病毒分離，並進行病毒分子特徵、抗原性與致病性之系統性評估。本研究從台灣田間雞場共分離到10株FAdV，來源涵蓋白肉雞、蛋雞、種雞及有色肉雞，全基因體定序結果顯示，病毒株分屬三個基因型（C、D、E）與五個血清型（FAdV-2、-3、-8a、-8b、-11），其中一株有跨型別基因重組現象。另外，血清交叉中和試驗結果證實各分離株具明顯的抗原性差異，顯示台灣FAdV流行株具高度抗原多樣性與複雜性。接著，本研究選用代表性毒株NTU/C1120/2023進行動物實驗，設計包含單一感染以及和傳染性支氣管炎病毒（infectious bronchitis virus, IBV）之共感染模式。共感染組雞隻表現出更嚴重的臨床症狀、更高的死亡率，並出現明顯的免疫組織與肝臟病變。雖然早期組織與血液中FAdV含量較低，但病毒排毒時間延長，且抗體反應受抑，並進一步以組織病理與免疫組織化學染色確認肝臟為主要病變器官。為了確認FAdV與IBV之間的干擾現象，以雞胚腎細胞進行共感染的細胞實驗，發現IBV可抑制早期FAdV複製，而FAdV則於感染後期促進IBV之複製。綜合本研究結果，顯示目前台灣流行之FAdV株具有高度基因與抗原多樣性，並與IBV存在時間依賴性之干擾現象，這些結果突顯了台灣持續監測FAdV與採取疾病防控策略之重要性。 | zh_TW |
| dc.description.abstract | Fowl adenoviruses (FAdVs) are important avian pathogens associated with inclusion body hepatitis and hydropericardium-hepatitis syndrome, contributing to significant economic losses in the poultry industry. Despite increasing cases in Taiwan, the comprehensive characterization of circulating strains remains limited. This study investigated the molecular features, antigenic properties, and pathogenicity of FAdVs isolated in Taiwan between 2020 and 2025. Among suspected cases from broilers, layers, breeders, and colored broilers farms, 10 FAdVs were isolated. Whole-genome sequencing revealed five serotypes (FAdV-2, -3, -8a, -8b, and -11) across three species (C, D, and E), with one isolate exhibiting intertypic recombination. Cross-neutralization assays confirmed distinct serotypes with minimal antigenic overlap, highlighting the complexity of FAdV circulation in Taiwan. A representative strain (NTU/C1120/2023) was selected for animal studies, including single and co-infection with infectious bronchitis virus (IBV) in SPF chickens. Co-infected birds exhibited more severe clinical signs, higher mortality, and pronounced lesions in lymphoid and liver tissues. Although FAdV loads in tissues and blood were initially lower in co-infected birds, viral shedding was prolonged, and antibody responses were reduced. Histopathology and IHC confirmed extensive hepatic involvement. In vitro studies using chicken embryo kidney cells demonstrated that IBV suppressed early FAdV replication, while FAdV enhanced IBV replication at later stages. Collectively, this study highlights the genetic and antigenic diversity of FAdVs in Taiwan and reveals a dynamic, time-dependent interaction between FAdV and IBV. These findings underscore the need for continuous surveillance and tailored vaccine strategies to mitigate FAdV-associated diseases in poultry. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-09-10T16:23:48Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-09-10T16:23:48Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 誌謝 .................................................................................................................................... i
中文摘要 ........................................................................................................................... ii ABSTRACT ..................................................................................................................... iii CONTENTS ...................................................................................................................... v LIST OF FIGURES ........................................................................................................ viii LIST OF TABLES ............................................................................................................ xi Chapter 1 Introduction .............................................................................................. 1 1.1 History and background of fowl adenovirus (FAdV) ..................................... 1 1.2 Structure and Classification of FAdV ............................................................ 2 1.3 Disease and epidemiology of FAdV ............................................................... 5 1.4 Diagnosis tools of FAdV ................................................................................ 7 1.5 Vaccination and control strategies .................................................................. 9 1.6 Co-infection status of FAdV ......................................................................... 11 1.7 Oxford nanopore sequencing ........................................................................ 12 1.8 Research on FAdV in Taiwan ....................................................................... 14 1.9 Rationale and objective of this study ........................................................... 15 Chapter 2 Materials and Methods ......................................................................... 17 2.1 Source of clinical cases ................................................................................ 17 2.2 Sample processing and virus detection ........................................................ 17 2.3 Source of serums .......................................................................................... 18 2.4 Enzyme-linked immunosorbent assay (ELISA) ........................................... 19 2.5 Virus isolation ............................................................................................... 19 2.6 Virus propagation ......................................................................................... 20 2.7 Virus titration ................................................................................................ 21 2.8 Transmission electron microscopy ............................................................... 21 2.9 Immunocytochemical (ICC) staining ........................................................... 22 2.10 Virus growth kinetics on LMH cells ............................................................ 23 2.11 Whole genome sequencing ........................................................................... 23 2.12 Phylogenetic analysis ................................................................................... 25 2.13 Recombination analysis ................................................................................ 26 2.14 Preparation of hyperimmune serum ............................................................. 26 2.15 Cross-neutralization test ............................................................................... 27 2.16 Establishment of a quantitative PCR (qPCR) and standard curve ............... 27 2.17 Pathogenicity examination ........................................................................... 29 2.18 Immunohistochemistry (IHC) staining ......................................................... 30 2.19 In vitro co-infection experiment in CEK cells ............................................. 31 2.20 Statistical analysis ........................................................................................ 32 Chapter 3 Results ..................................................................................................... 33 3.1 Sample collection ......................................................................................... 33 3.2 Serological detection of FAdV antibodies in poultry farms ......................... 33 3.3 Virus isolation ............................................................................................... 34 3.4 Virus titration ................................................................................................ 35 3.5 Morphological observation by transmission electron microscopy ............... 35 3.6 Cytopathic effects and immunocytochemical confirmation of FAdV infection in LMH cells ................................................................................................. 36 3.8 Whole genome sequencing and annotation of FAdV isolates ...................... 38 3.9 Phylogenetic analysis ................................................................................... 39 3.10 Recombination analysis ................................................................................ 41 3.11 Hyperimmune antibody generation .............................................................. 42 3.12 qPCR and standard curve for FAdV ............................................................. 43 3.13 Pathogenicity examination ........................................................................... 44 3.14 In vitro co-infection experiment in CEK cells ............................................. 50 Chapter 4 Discussion ............................................................................................... 53 Chapter 5 Figures and tables .................................................................................. 59 Chapter 6 References ............................................................................................. 118 | - |
| dc.language.iso | en | - |
| dc.subject | 全基因體定序 | zh_TW |
| dc.subject | 家禽腺病毒 | zh_TW |
| dc.subject | 共感染 | zh_TW |
| dc.subject | 病原性試驗 | zh_TW |
| dc.subject | 抗原性分析 | zh_TW |
| dc.subject | Antigenicity | en |
| dc.subject | Co-infection | en |
| dc.subject | Whole genome sequencing | en |
| dc.subject | Fowl adenovirus | en |
| dc.subject | Pathogenicity | en |
| dc.title | 台灣家禽腺病毒之基因、抗原性與致病性分析 | zh_TW |
| dc.title | Characterization of fowl adenovirus isolated in Taiwan: genome, antigenicity, and pathogenicity | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 王金和;鄭明珠 | zh_TW |
| dc.contributor.oralexamcommittee | Ching-Ho Wang;Ming-Chu Cheng | en |
| dc.subject.keyword | 家禽腺病毒,全基因體定序,抗原性分析,病原性試驗,共感染, | zh_TW |
| dc.subject.keyword | Fowl adenovirus,Whole genome sequencing,Antigenicity,Pathogenicity,Co-infection, | en |
| dc.relation.page | 123 | - |
| dc.identifier.doi | 10.6342/NTU202501958 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2025-07-20 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 獸醫學系 | - |
| dc.date.embargo-lift | N/A | - |
| 顯示於系所單位： | 獸醫學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-113-2.pdf 未授權公開取用 | 23.12 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。