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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/98226完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 萬灼華 | zh_TW |
| dc.contributor.advisor | Cho-Hua Wan | en |
| dc.contributor.author | 丘星筠 | zh_TW |
| dc.contributor.author | Celine Suhakam | en |
| dc.date.accessioned | 2025-07-30T16:24:35Z | - |
| dc.date.available | 2025-07-31 | - |
| dc.date.copyright | 2025-07-30 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-07-28 | - |
| dc.identifier.citation | References
1. Albers TM, Henderson KS, Mulder GB, Shek WR. Pathogen prevalence estimates and diagnostic methodology trends in laboratory mice and rats from 2003 to 2020. Journal of the American Association for Laboratory Animal Science. 2023 May 1;62(3):229-42. 2. Beckwith CS, Franklin CL, Hook Jr RR, Besch-Williford CL, Riley LK. Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents. Journal of Clinical Microbiology. 1997 Jun;35(6):1620-3. 3. Bostick JW, Wang Y, Shen Z, Ge Y, Brown J, Chen ZM, Mohamadzadeh M, Fox JG, Zhou L. Dichotomous regulation of group 3 innate lymphoid cells by nongastric Helicobacter species. Proceedings of the National Academy of Sciences. 2019 Dec 3;116(49):24760-9. 4. Bracken TC, Cooper CA, Ali Z, Truong H, Moore JM. Helicobacter infection significantly alters pregnancy success in laboratory mice. Journal of the American Association for Laboratory Animal Science. 2017 May 1;56(3):322-9. 5. Burich A, Hershberg R, Waggie KI, Zeng W, Brabb T, Westrich G, Viney JL, Maggio-Price L. Helicobacter-induced inflammatory bowel disease in IL-10-and T cell-deficient mice. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2001 Sep 1;281(3):G764-78. 6. Cao S, Zhu C, Feng J, Zhu L, Yin J, Xu Y, Yang H, Huang Y, Zhang Q. Helicobacter hepaticus infection induces chronic hepatitis and fibrosis in male BALB/c mice via the activation of NF‐κB, Stat3, and MAPK signaling pathways. Helicobacter. 2020 Apr;25(2):e12677. 7. Chichlowski M, Sharp JM, Vanderford DA, Myles MH, Hale LP. Helicobacter typhlonius and Helicobacter rodentium differentially affect the severity of colon inflammation and inflammation-associated neoplasia in IL10-deficient mice. Comparative Medicine. 2008 Dec 15;58(6):534-41. 8. Council of Agriculture. Animal Protection Act. ROC, 2021. Available at: https://law.moa.gov.tw/EngLawContent.aspx?lan=E&id=411&KW=Animal+Protecti on+Act 9. Council of Agriculture. Guideline for the Care and Use of Laboratory Animals. Chinese Taipei Society of Laboratory Animal Sciences, Taipei, ROC, 2018. 10. del Carmen Martino-Cardona M, Beck SE, Brayton C, Watson J. Eradication of Helicobacter spp. by using medicated diet in mice deficient in functional natural killer cells and complement factor D. Journal of the American Association for Laboratory Animal Science. 2010 May 15;49(3):294-9. 11. Drazenovich NL, Franklin CL, Livingston RS, Besselsen DG. Detection of rodent Helicobacter spp. by use of fluorogenic nuclease polymerase chain reaction assays. Comparative Medicine. 2002 Aug 15;52(4):347-53. 12. Erdman SE, Poutahidis T, Tomczak M, Rogers AB, Cormier K, Plank B, Horwitz BH, Fox JG. CD4+ CD25+ regulatory T lymphocytes inhibit microbially induced colon cancer in Rag2-deficient mice. The American Journal of Pathology. 2003 Feb 1;162(2):691-702. 13. Farell EM, Alexandre G. Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates. BMC research notes. 2012 Dec;5:1-8. 14. Feng S, Ku K, Hodzic E, Lorenzana E, Freet K, Barthold SW. Differential detection of five mouse-infecting helicobacter species by multiplex PCR. Clinical and Vaccine Immunology. 2005 Apr;12(4):531-6. 15. Fox JG, Yan LL, Dewhirst FE, Paster BJ, Shames B, Murphy JC, Hayward A, Belcher JC, Mendes EN. Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice. Journal of Clinical Microbiology. 1995 Feb;33(2):445-54. 16. Fox JG, Li X, Yan L, Cahill RJ, Hurley R, Lewis R, Murphy JC. Chronic proliferative hepatitis in A/JCr mice associated with persistent Helicobacter hepaticus infection: a model of helicobacter-induced carcinogenesis. Infection and Immunity. 1996 May;64(5):1548-58. 17. Fox JG, MacGregor JA, Shen Z, Li X, Lewis R, Dangler CA. Comparison of methods of identifying Helicobacter hepaticus in B6C3F1 mice used in a carcinogenesis bioassay. Journal of Clinical Microbiology. 1998 May 1;36(5):1382-7. 18. Fox JG, Rogers AB, Whary MT, Taylor NS, Xu S, Feng Y, Keys S. Helicobacter bilis-associated hepatitis in outbred mice. Comparative Medicine. 2004 Oct 15;54(5):571-7. 19. Fox JG and Whary MT. Helicobacter Infections in Mice. In: Fox JG, Barthold SW, Davisson MT, Newcomer CE, Quimby FW, Smith AL. (2007). The Mouse in Biomedical Research: Diseases (Vol. 2). Elsevier. (pp 407-435). 20. Franklin CL, Riley LK, Livingston RS, Beckwith CS, Besch-Williford CL, Hook Jr RR. Enterohepatic lesions in SCID mice infected with Helicobacter bilis. Comparative Medicine. 1998 Aug 1;48(4):334-9. 21. Franklin CL, Riley LK, Livingston RS, Beckwith CS, Hook RR, Besch-Williford CL, Hunziker R, Gorelick PL. Enteric lesions in SCID mice infected with “Helicobacter typhlonicus,” a novel urease-negative Helicobacter species. Comparative Medicine. 1999 Oct 1;49(5):496-505. 22. Hailey JR, Haseman JK, Bucher JR, Radovsky E, Malarkey DE, Miller RT, Nyska A, Maronpot RR. Impact of Helicobacter hepaticus infection in B6C3F1 mice from twelve National Toxicology Program two-year carcinogenesis studies. Toxicologic Pathology. 1998 Sep;26(5):602-11. 23. Henderson KS, Perkins CL, Havens RB, Kelly MJ, Francis BC, Dole VS, Shek WR. Efficacy of direct detection of pathogens in naturally infected mice by using a high-density PCR array. Journal of the American Association for Laboratory Animal Science. 2013 Nov 1;52(6):763-72. 24. Höfler D, Nicklas W, Mauter P, Pawlita M, Schmitt M. A bead-based multiplex assay for the detection of DNA viruses infecting laboratory rodents. PLoS One. 2014 May 16;9(5):e97525. 25. Jeon WJ, Dong HJ, Shin JH, Kim IY, Ho H, Oh SH, Yoon YM, Choi YK, Suh JG, Nam KH, Kim HC. Helicobacter apodemus sp. nov., a new Helicobacter species identified from the gastrointestinal tract of striped field mice in Korea. Journal of Veterinary Science. 2015 Dec 1;16(4):475-81. 26. Jergens AE, Wilson-Welder JH, Dorn A, Henderson A, Liu Z, Evans RB, Hostetter J, Wannemuehler MJ. Helicobacter bilis triggers persistent immune reactivity to antigens derived from the commensal bacteria in gnotobiotic C3H/HeN mice. Gut. 2007 Jul 1;56(7):934-40. 27. Kostomitsopoulos N, Donnelly H, Kostavasili I, Paronis E, Alexakos P, Karayannacos P. Eradication of Helicobacter bilis and H. hepaticus from infected mice by using a medicated diet. Lab Animal. 2007 May;36(5):37-40. 28. Livingston RS, Myles MH, Livingston BA, Criley JM, Franklin CL. Sex influence on chronic intestinal inflammation in Helicobacter hepaticus-infected A/JCr mice. Comparative Medicine. 2004 Jun 15;54(3):301-8. 29. Maggio-Price L, Shows D, Waggie K, Burich A, Zeng W, Escobar S, Morrissey P, Viney JL. Helicobacter bilis infection accelerates and H. hepaticus infection delays the development of colitis in multiple drug resistance-deficient (mdr1a−/−) mice. The American Journal of Pathology. 2002 Feb 1;160(2):739-51. 30. Maggio-Price L, Treuting P, Zeng W, Tsang M, Bielefeldt-Ohmann H, Iritani BM. Helicobacter infection is required for inflammation and colon cancer in SMAD3-deficient mice. Cancer Research. 2006 Jan 15;66(2):828-38. 31. Miller CL, Muthupalani S, Shen Z, Fox JG. Isolation of Helicobacter spp. from mice with rectal prolapses. Comparative Medicine. 2014 Jun 1;64(3):171-8. 32. Myles MH, Livingston RS, Livingston BA, Criley JM, Franklin CL. Analysis of gene expression in ceca of Helicobacter hepaticus-infected A/JCr mice before and after development of typhlitis. Infection and Immunity. 2003 Jul;71(7):3885-93. 33. Nagamine CM, Sohn JJ, Rickman BH, Rogers AB, Fox JG, Schauer DB. Helicobacter hepaticus infection promotes colon tumorigenesis in the BALB/c-Rag2−/− Apc Min/+ mouse. Infection and Immunity. 2008 Jun;76(6):2758-66. 34. Neubert V, Sadek A, Burell T, Ralser A, Erhard M, Gerhard M, Seidel K, Kalali B. Validation and improvement of a multiplex PCR method to detect murine Helicobacter species in feces samples of mice. Helicobacter. 2022 Jun;27(3):e12888. 35. Nguyen DD, Muthupalani S, Goettel JA, Eston MA, Mobley M, Taylor NS, McCabe A, Marin R, Snapper SB, Fox JG. Colitis and colon cancer in WASP-deficient mice require helicobacter species. Inflammatory Bowel Diseases. 2013 Sep 1;19(10):2041-50. 36. Pritchett-Corning KR, Cosentino J, Clifford CB. Contemporary prevalence of infectious agents in laboratory mice and rats. Laboratory Animals. 2009 Apr;43(2):165-73.] 37. Rao VP, Poutahidis T, Ge Z, Nambiar PR, Boussahmain C, Wang YY, Horwitz BH, Fox JG, Erdman SE. Innate immune inflammatory response against enteric bacteria Helicobacter hepaticus induces mammary adenocarcinoma in mice. Cancer Research. 2006 Aug 1;66(15):7395-400. 38. Shames B, Fox JG, Dewhirst F, Yan L, Shen Z, Taylor NS. Identification of widespread Helicobacter hepaticus infection in feces in commercial mouse colonies by culture and PCR assay. Journal of Clinical Microbiology. 1995 Nov;33(11):2968-72. 39. Sharp JM, Vanderford DA, Chichlowski M, Myles MH, Hale LP. Helicobacter infection decreases reproductive performance of IL10-deficient mice. Comparative Medicine. 2008 Oct 15;58(5):447-53. 40. Shen Z, Schauer DB, Mobley HL, Fox JG. Development of a PCR-restriction fragment length polymorphism assay using the nucleotide sequence of the Helicobacter hepaticus urease structural genes ureAB. Journal of Clinical Microbiology. 1998 Sep 1;36(9):2447-53. 41. Sint D, Raso L, Traugott M. Advances in multiplex PCR: balancing primer efficiencies and improving detection success. Methods in Ecology and Evolution. 2012 Oct;3(5):898-905. 42. Shomer NH, Dangler CA, Marini RP, Fox JG. Helicobacter bilis/Helicobacter rodentium co-infection associated with diarrhea in a colony of scid mice. Comparative Medicine. 1998 Oct 1;48(5):455-9. 43. Stout MD, Kissling GE, Suárez FA, Malarkey DE, Herbert RA, Bucher JR. Influence of Helicobacter hepaticus infection on the chronic toxicity and carcinogenicity of triethanolamine in B6C3F1 mice. Toxicologic Pathology. 2008 Oct;36(6):783-94. 44. Ward JM, Fox JG, Anver MR, Haines DC, George CV, Collins Jr MJ, Gorelick PL, Nagashima K, Gonda MA, Gilden RV, Tully JG. Chronic active hepatitis and associated liver tumors in mice caused by a presistent bacterial infection with a novel Helicobacter Species. JNCI: Journal of the National Cancer Institute. 1994 Aug 17;86(16):1222-7. 45. Whary MT, Fox JG. Detection, eradication, and research implications of Helicobacter infections in laboratory rodents. Lab Animal. 2006 Jul;35(7):25-3 | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/98226 | - |
| dc.description.abstract | 螺旋桿菌屬（Helicobacter spp.）是實驗小鼠最常見的病原之一。多種囓齒動物致病性螺旋桿菌，包括H. hepaticus、H. bilis 和H. typhlonius，會影響免疫反應、有致癌性，並且可以改變生殖相關數據。目前，螺旋桿菌屬的PCR檢測已經應用在例行性小鼠健康監測。然而，目前臺灣尚無針對致病性的螺旋桿菌有PCR檢測方法。在本研究中，我們針對四種致病性螺旋桿菌，包含H. hepaticus、H. bilis、H. typhlonius及H. apodemus，以及大小鼠的管家基因，建立了一個多重引子聚合酶鏈鎖反應（Helicobacters/Actin multiplex PCR assay）。此Helicobacters/Actin多重引子聚合酶鏈鎖反應可特異性的增幅出四種螺旋桿菌的目標基因，並且能針對所有目標的螺旋桿菌之偵測極限達到50 copies 的高敏感性。為了調查這四種螺旋桿菌在台灣實驗小鼠的感染情況，本研究採用此Helicobacters/Actin多重引子聚合酶鏈鎖反應，針對來自10個不同研究機構119隻小鼠的糞便樣本進行感染情況的調查。此結果顯示，在10個不同的研究機構中有7個單位有這些致病性螺旋桿菌的感染，其感染率分別為： H. hepaticus（23.53%）、H. bilis（0%）、H. typhlonius（17.65%）和 H. apodemus（12.61%）。其中，含有多種螺旋桿菌共同感染情況分別為H. hepaticus/H. typhlonius（感染率為5.88%）和H. hepaticus/H. typhlonius/H. apodemus （感染率為8.40%）。並且，此多重引子聚合酶鏈鎖反應具有高敏感性（100%），高特異性（98.9% - 100%），高準確性（99.16% - 100%）。本研究開發的Helicobacters/Actin多重引子聚合酶鏈鎖反應在不需要犧牲動物及干擾動物的前提下，即可同時由實驗小鼠的糞便中，同時偵測四種致病性螺旋桿菌的感染 | zh_TW |
| dc.description.abstract | Helicobacter spp. is one of the most prevalent pathogens in laboratory mouse colonies. Several rodent helicobacters, including Helicobacter hepaticus, H. bilis, and H. typhlonius can modulate immunological responses, carcinogenesis, and reproduction related data. Currently, helicobacter generic PCR assays have been well applied in routine health monitoring of laboratory mice. However, species-specific PCR assays for pathogenic helicobacters are still not available in Taiwan. In this study, we developed a species-specific Helicobacters/Actin multiplex PCR assay to simultaneously detect and differentiate four helicobacter species (H. hepaticus, H. bilis, H. typhlonius, and H. apodemus) and a housekeeping gene in feces. The multiplex PCR assay could detect an equivalent infection of four helicobacter species, with a detection limit as few as 50 copies. To survey the infection status of these four helicobacter species, a total of 119 mouse fecal samples collected from 10 different research facilities were screened by this multiplex PCR assay. The prevalence surveillance result revealed that 7 out of 10 different research facilities were positive for helicobacters and the infection rates were as followed: H. hepaticus (23.53%), H. bilis (0%), H. typhlonius (17.65%), and H. apodemus (12.61%). In addition, multiple co-infection rates of helicobacters species were 5.88% (H. hepaticus/H. typhlonius) and 8.40% (H. hepaticus/H. typhlonius/H. apodemus). This multiplex PCR assay has a high sensitivity (100%), specificity (98.9% - 100%), and accuracy (99.16% - 100%). The Helicobacters/Actin multiplex PCR assay developed in this study would be a powerful tool to screen pathogenic helicobacter infections in laboratory mouse colonies without animal sacrifice. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-07-30T16:24:35Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-07-30T16:24:35Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | TABLE OF CONTENTS
口試委員會審定書………………………………………………………………………………………# ACKNOWLEDGEMENTS……………………………………………………………………………....I ABSTRACT IN CHINESE……………………………………………………………………………....II ABSTRACT IN ENGLISH……………………………………………………………………………..III TABLE OF CONTENTS……………………………………………………………………………….IV LIST OF TABLES ..…………………………………………………………………………………......V LIST OF FIGURES……………………………………………………………………………………..VI INTRODUCTION……………………………………………………………………………………….1 METHODS AND MATERIALS………………………………………………………………………..7 Collection of Animals and Samples……………………………………………………………..7 Fecal DNA and Helicobacter DNA (DNA extraction).…………………………………………7 PCR Analysis……………………………………………………………………………………8 DNA Sequencing………………………………………………………………………………..9 RESULTS………………………………………………………………………………………………11 Helicobacter Species-specific Primers Evaluation……………………………………………..11 Specificity and Detection Limit of the Helicobacter Species-specific Single PCR……………11 Specificity and Detection Limit of the Helicobacters/Actin Multiplex PCR assay……………12 Surveillance for the Pathogenic Helicobacter Infections with the Helicobacters/Actin Multiplex PCR assay……………………………………………………………………………………...13 DISCUSSION………………………………………………………………………………………….15 REFERENCES…………………………………………………………………………………………37 | - |
| dc.language.iso | en | - |
| dc.subject | 致病性螺旋桿菌 | zh_TW |
| dc.subject | H. hepaticus | zh_TW |
| dc.subject | H. bilis | zh_TW |
| dc.subject | H. typhlonius | zh_TW |
| dc.subject | H. apodemus | zh_TW |
| dc.subject | 多重引子聚合酶鏈鎖反應 | zh_TW |
| dc.subject | H. hepaticus | en |
| dc.subject | multiplex PCR | en |
| dc.subject | H. apodemus | en |
| dc.subject | H. typhlonius | en |
| dc.subject | H. bilis | en |
| dc.title | 實驗小鼠致病性螺旋桿菌 多重引子聚合酶鏈鎖反應之建立 | zh_TW |
| dc.title | Establishment of a Helicobacters/Actin Multiplex PCR Assay for the Detection of Pathogenic Helicobacters in Laboratory Mice | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 廖欽峯;謝淑貞;廖泰慶 | zh_TW |
| dc.contributor.oralexamcommittee | Ching-Fong Liao;Shu-Chen Hsieh;Albert Tai-Ching Liao | en |
| dc.subject.keyword | 致病性螺旋桿菌,H. hepaticus,H. bilis,H. typhlonius,H. apodemus,多重引子聚合酶鏈鎖反應, | zh_TW |
| dc.subject.keyword | H. hepaticus,H. bilis,H. typhlonius,H. apodemus,multiplex PCR, | en |
| dc.relation.page | 42 | - |
| dc.identifier.doi | 10.6342/NTU202502716 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2025-07-29 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 分子暨比較病理生物學研究所 | - |
| dc.date.embargo-lift | 2025-07-31 | - |
| 顯示於系所單位： | 分子暨比較病理生物學研究所 | |
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