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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 李篤中(Duu-Jong Lee) | |
dc.contributor.author | Kun-Jou Chen | en |
dc.contributor.author | 陳坤柔 | zh_TW |
dc.date.accessioned | 2021-05-20T20:41:32Z | - |
dc.date.available | 2013-07-24 | |
dc.date.available | 2021-05-20T20:41:32Z | - |
dc.date.copyright | 2008-07-24 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-22 | |
dc.identifier.citation | 陳明源, 2006. 多重染色方案-胞外聚合物於生物聚集體之分佈,碩士論文,國立台灣大學化學工程研究所,台北
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El-Mamouni R., Leduc R., Guiot SR., 1998. Influence of synthetic and natural polymers on the anaerobic granulation process. Water Sci Technol. 38(8-9), 341–347. ERIKSSON, L., ALM, B., 1991. Study of flocculation mechanisms by observing effects of a complexing agent on activated-sludge properties. WATER SCIENCE AND TECHNOLOGY. 24(7), 21-28. Frolund B., Palmgren R., Keiding K., Nielsen PH., 1996. Extraction of extracellular polymers from activated sludge using a cation exchange resin. WATER RESEARCH. 30(8), 1749-1758. Jiang HL., Tay JH., Tay STL., 2002.Aggregation of immobilized activated sludge cells into aerobically grown microbial granules for the aerobic biodegradation of phenol. Lett Appl Microbiol. 35(5), 439–445. Jiang HL., Tay JH., Liu Y., Tay STL., 2003. Ca2 + augmentation for enhancement of aerobically grown microbial granules in sludge blanket reactors. Biotechnol Lett. 25, 95- 99. Keweloh H., Heipieper H.J., and Rehm H.J., 1989. Protection of bacteria against toxicity of phenol by immobilization in calcium alginate. Appl. Microbiol. Biotechnol. 31, 383–389. Lili Zhang, Xinxing Feng, Nanwen Zhuc, Jianmeng Chen, 2007. Role of extracellular protein in the formation and stability of aerobic granules. Enzyme and Microbial Technology. 41, 551–557. Liu H., and Fang H.H.P., 2002. Characterization of electrostatic binding sites of extracellular polymers by linear programming analysis of titration data. Biotechnol. Bioeng. 80, 806-811. Liu Y.Q., Liu Y., and Tay J.H., 2004. The effects of extracellular polymeric substances on the formation and stability of biogranules. Appl. Microbiol. Biot. 65, 143-148. Matthew JH., John TN., 1997. Characterization of exocellular protein and its role in bioflocculation. J Environ Eng. 123(5), 479–85. McSwain BS., Irvine RL., Hausner M., Wilderer PA., 2005. Composition and Distribution of Extracellular Polymeric Substances in Aerobic Flocs and Granular Sludge. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 1051–1057. Morgenroth, E., T. Sherden., M. C. M. van Loosdrecht., J. J. Heijnen., and P. A. Wilderer., 1997. Aerobic granular sludge in a sequencing batch reactor. Water Res. 31, 3191–3194. Moy BYP., Tay JH., Toh SK., Liu Y., Tay STL.,2002. High organic loading influences the physical characteristics of aerobic sludge granules. Lett Appl Microbiol. 34(6), 407– 412. Ohashi A., Harada H., 1994. Adhesion strength of biofilm developed in an attached-growth reactor. Water Sci Technol. 29(10-11), 281-288. Peng D., Bernet N., Delgenes JP., Moletta R., 1999. Aerobic granular sludge—a case report. Water Res. 33, 890–893. Qin L., Tay JH., Liu Y., 2004. Selection pressure is a driving force of aerobic granulation in sequencing batch reactors. Process Biochem. 39, 579–584. Schmidt JE., Ahring BK., 1996. Granular sludge formation in upflow anaerobic sledge blanket (UASB) reactors. BIOTECHNOLOGY AND BIOENGINEERING. 49(3), 229-246. Shin HS., Lim KH., Park HS., 1992. Effect of shear stress on granulation in oxygen aerobic upflow sludge reactors. Water Sci Technol. 26, 601–605. Sunil S. Adav, Chen MY, Lee DJ, Ren NQ, 2007. Degradation of phenol by aerobic granules and isolated yeast Candida tropicalis. BIOTECHNOLOGY AND BIOENGINEERING. 96(5), 844-852. Sunil S. Adav, Duu-Jong Lee, Joo-Hwa Tay, 2008. Extracellular polymeric substances and structural stability of aerobic granule. Water Res. 42, 1644-1650. Tay JH., Liu QS., Liu Y., 2001a. Microscopic observation of aerobic granulation in sequential aerobic sludge blanket reactor. J Appl Microbiol. 91(1), 168– 175. Tay JH., Liu QS., Liu Y., 2001c. The role of cellular polysaccharides in the formation and stability of aerobic granules. Lett Appl Microbiol. 33(3), 222–226. Tay JH., Yang SF., Liu Y., 2002b. Hydraulic selection pressure-induced nitrifying granulation in sequencing batch reactors. Appl Microbiol Biotechnol. 59, 332–337. Tay JH., Ivanov V., Pan S., Tay STL., 2002d. Specific layers in aerobically grown microbial granules. Lett Appl Microbiol. 34(4), 254–257. Tay JH., Tay STL., Ivanov V., Pan S., Liu QS., 2003a. Biomass and porosity profile in microbial granules sued for aerobic wastewater treatment. Lett Appl Microbiol. 36(5), 297–301. Toh SK., Tay JH., Moy BYP., Ivanov V., Tay STL., 2003. Size-effect on the physical characteristics of the aerobic granule in a SBR. Appl Microbiol Biotechnol. 60, 687–695. Urbain V., Block JC., Manem J., 1993. Bioflocculation in activated sludge, an analytic approach. Water Res. 27, 829–38. Wang Z.W., Liu Y., and Tay J.H., 2005. Distribution of EPS and cell surface hydrophobicity in aerobic granules. Appl Microbiol Biotechnol. 69, 469-473. Zhen-Peng Zhang, Kuan-Yeow Show, Joo-Hwa Tay, David Tee Liang, Duu-Jong Lee, Wen-Ju Jiang, 2006. Effect of hydraulic retention time on biohydrogen production and anaerobic microbial community. Process Biochemistry. 41, 2118–2123 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9790 | - |
dc.description.abstract | 生物顆粒(granule)是經由細胞自身與細胞外間質(extracellular polymeric substances, EPS)聚集而成近似圓球的生物聚集體。EPS可作細胞之間的架橋,具有強化生顆粒結構的功能,而EPS組成多為蛋白質、多醣類及脂質。研究指出,正二價及正三價金屬陽離子能加速顆粒形成,例如Ca2+。
本論文採用3種生物顆粒(granule)作為測試樣品,分別為好氧酚顆粒、厭氧產氫顆粒及厭氧產甲烷顆粒。顆粒中加入螫合劑EDTA、α- amylase、β- amylase、proteinase K、lipase、cellulase,藉由螢光探針雜交顆粒中的多醣體、脂質、蛋白質,結合雷射共軛焦顯微鏡(Confocal Laser Scanning Microscope)掃描,探討金屬陽離子、蛋白質、脂質、多醣體及纖維素對顆粒粒結構穩定性影響。 結果顯示金屬陽離子、多醣體、脂質及蛋白質對酚好氧顆粒、厭氧產氫顆粒及厭氧產甲烷顆粒結構強度不造成影響,纖維素對酚好氧顆粒及厭氧產甲烷顆粒結構強度不造成影響,但是纖維素是厭氧產氫顆粒網狀結構的支幹物質。 | zh_TW |
dc.description.abstract | Granules can be described as a collection of sel-immobilized cells and extracellular polymeric substances (EPS) into a somewhat spherical form. EPS can mediate both cohesion and adhesion of cells and play a crucial role in maintaining the structural integrity in a community of immobilized cell and the composition of EPS include proteins, polysaccharides and lipid .In recently research, the presence of divalent and trivalent cations ions, such as Ca2 + etc, helps cells to form microbial nuclei that promote further granulation
The investigation into structural stability of three biochemical granules (one aerobic granules and two anaerobic granules) were examined. Selective enzymes of proteins, lipids, proteinase K, α- amylase, β- amylase, cellulase and EDTA were added to those three granules to influence structural stability. The roles of proteins,α- and β-polysaccharides, and lipids were studied via their selective hydrolysis using enzymes and structural changes of granule were probed using in situ fluorescent staining and confocal laser scanning microscopy. The results show elective enzymes of proteins, lipids, proteinase K, α amylase, β amylase and EDTA are not backbone of those three granules.Also, cellulose are not backbone of methane production by anaerobic granules and Degradation of Phenol by Aerobic Granules. However, cellulose form the backbone of a network-like outer layer with embedded proteins, lipids, a-polysaccharides, and cells to support the mechanicalstability of structural integrity of hydrogen production by anaerobic granules. | en |
dc.description.provenance | Made available in DSpace on 2021-05-20T20:41:32Z (GMT). No. of bitstreams: 1 ntu-97-R92524065-1.pdf: 5206864 bytes, checksum: 924abba8394389f8222a085e78324e44 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 目錄
頁次 中文摘要 ………………………………………………………………….……...…I 英文摘要 ………………………………………………………………………..… II 目錄 ……………………………………………………………………….….…III 圖目錄 ………………………………………………………………………….…. V 表目錄 ……………………………....………………………………………..…. VII 第一章 前言………………………………………………………………………. 1 第二章 文獻回顧…………………………………………………………………..2 2-1 膠羽和顆粒…………………………………………………………………...2 2-2 生物顆粒的特性與形成…………….………………………………………..2 2-3 細胞外間質和生物顆粒的關係…………..…………...…………..…………3 2-4 細胞外間質的萃取與分佈…………………...……………………………....3 2-5 好氧顆粒之穩定性…………………………………………………………...4 第三章 樣品與實驗………………………………………..……………………....6 3-1 污泥來源……….………………………………………………………….…..6 3-2 顆粒來源……….……………………………………………………….…......6 3-3 顆粒結構強度測試…………………………………………………………...8 3-3-1 實驗藥品…………………………………………………………….8 3-3-2 生物顆粒降解測試………………………………………………….9 3-4 螢光染色方法……………………………………………………………….10 3-4-1 實驗染劑……………………………………………………….….....10 3-4-2 染色方法……………………………………………………….….....10 第四章 結果與討論………………………………………………………………..12 4-1 顆粒結構穩定性檢驗………………………………………………..…..….12 4-2 超音波震盪………………………………………………………………….13 4-3 EDTA螯合顆粒鈣離子…………………………………………………….14 4-4 粒徑分布實驗……………………………………………………….…..…..15 4-5 五重染色: 酚好氧顆粒.…………………………..……….………………18 4-6 五重染色: 厭氧產氫顆粒……………………………………….…………31 4-7 五重染色: 厭氧產甲烷顆粒.………………..……………..……….…...…44 第五章 結論…………..…………………………………………………………..57 參考文獻……………………………………………………………………………..58 附錄(一) 酵素特性….................................................................................................62 圖目錄 圖2-1 好氧顆粒加β-amylase之共軛焦顯微鏡圖和螢光強度分析圖……………4 圖4-1 EDTA螯合顆粒鈣離子…………………………………………………….14 圖4-2 酚好氧顆粒加cellulase粒徑分佈…………………………………………15 圖4-3 厭氧產氫顆粒加cellulase粒徑分佈..……………………………..………16 圖4-4 厭氧產甲烷顆粒加cellulase粒徑分佈..…………………………..………17 圖4-5 酚好氧顆粒對照組………………………………………..……….……….19 圖4-6 酚好氧顆粒對照組螢光強度分析………………………………………....20 圖4-7 酚好氧顆粒加proteinase K………………………………………………...21 圖4-8 酚好氧顆粒加proteinase K螢光強度分析………………………………..22 圖4-9 酚好氧顆粒加α- amylase………………..………………………...….........23 圖4-10 酚好氧顆粒加α- amylase螢光強度分析……………………...………….24 圖4-11 酚好氧顆粒加β- amylase…………..……………………………...……...25 圖4-12 酚好氧顆粒加β- amylase螢光強度分析……………………………........26 圖4-13 酚好氧顆粒加lipase………………………………………………………27 圖4-14 酚好氧顆粒加lipase螢光強度分析………………………………………28 圖4-15 酚好氧顆粒加cellulase……………………………………………………29 圖4-16 酚好氧顆粒加cellulase螢光強度分析…………………………………..30 圖4-17 厭氧產氫顆粒對照組…..……………………..……………………..…....32 圖4-18 厭氧產氫顆粒對照組螢光強度分析…………………………….….……33 圖4-19 厭氧產氫顆粒加proteinase K…………………………………….………34 圖4-20 厭氧產氫顆粒加proteinase K螢光強度分析…………………………….35 圖4-21 厭氧產氫顆粒加α- amylase………………………………….………...…36 圖4-22 厭氧產氫顆粒加α- amylase螢光強度分析…………...………...………..37 圖4-23 厭氧產氫顆粒加β- amylase…………………………...………………….38 圖4-24 厭氧產氫顆粒加β- amylase螢光強度分析………………...………….…39 圖4-25 厭氧產氫顆粒加lipase…………………………………………..…….….40 圖4-26 厭氧產氫顆粒加lipase螢光強度分析……………………………………41 圖4-27 厭氧產氫顆粒加cellulase…….……………………………………...........42 圖4-28 厭氧產氫顆粒加cellulase螢光強度分析………………………………...43 圖4-29 厭氧產甲烷顆粒對照組….……………………….....…………………....45 圖4-30 厭氧產甲烷顆粒對照組螢光強度分析…………………………………..46 圖4-31 厭氧產甲烷顆粒加proteinase K……………..………………...………....47 圖4-32 厭氧產甲烷顆粒加proteinase K螢光強度分析………………………….48 圖4-33 厭氧產甲烷顆粒加α- amylase…………………..………..….......……….49 圖4-34 厭氧產甲烷顆粒加α- amylase螢光強度分析……………..……………..50 圖4-35 厭氧產甲烷顆粒加β- amylase………………………………………...….51 圖4-36 厭氧產甲烷顆粒加β- amylase螢光強度分析……………………………52 圖4-37 厭氧產甲烷顆粒加lipase………………………..........................…….….53 圖4-38 厭氧產甲烷顆粒加lipase螢光強度分析…………………………………54 圖4-39 厭氧產甲烷顆粒加cellulase………………………………………………55 圖4-40 厭氧產甲烷顆粒加cellulase螢光強度分析 …………………………….56 表目錄 表 3-1 酚顆粒培養基成份…………………………………....……………………...6 表 3-2 厭氧產氫顆粒Micronutrients成份…………..........................………….... ...6 表 3-3 厭氧產氫顆粒培養基成份…………………………………………………...7 表 3-4 厭氧產氫顆粒Micronutrients成份…………………………………………..7 表 3-5 厭氧產甲烷烷顆粒培養基成分……………………………………………...7 表 3-6厭氧產甲烷烷顆粒在UASB中操作條件……………………………………8 表 4-1顆粒和EDTA、五種酵素反應……………………………………………...12 表 4-2顆粒經超音波震盪結果……………………………………………………..13 | |
dc.language.iso | zh-TW | |
dc.title | 生物顆粒之穩定性 | zh_TW |
dc.title | Structural stability of biological granules | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃志彬,劉志成,鄒光耀(Kuan-Yeow Show),朱曉萍 | |
dc.subject.keyword | 顆粒,穩定性, | zh_TW |
dc.subject.keyword | granules,stability, | en |
dc.relation.page | 63 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2008-07-23 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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