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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃鵬林(Pung-Ling Huang) | |
| dc.contributor.author | Hsiang-You Kan | en |
| dc.contributor.author | 甘祥佑 | zh_TW |
| dc.date.accessioned | 2021-05-20T20:40:16Z | - |
| dc.date.available | 2016-08-01 | |
| dc.date.available | 2021-05-20T20:40:16Z | - |
| dc.date.copyright | 2011-09-08 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-09 | |
| dc.identifier.citation | 邱輝龍、許圳塗. 2005. 香蕉作物栽培種之起源. 臺灣園藝 51: 1-12.
Alexander, D. C. 1987. An efficient vector-primer cDNA cloning system. Meth. Enzymol. 154: 41-64. Amin, J., J. Ananthan, and R. Voellmy. 1988. Key features of heat shock regulatory elements. Mol. Cell. Biol. 8: 3761-3769. Argüello-Astorga, C. R. and L. R. Herrera-Estrella. 1996. Ancestral multipartite units in light-responsive plant promoters have structural features correlating with specific phototransduction pathways. Plant Physiol. 112: 1151-1166. Bai, F. and D. A. DeMason. 2006. Hormone interactions and regulation of unifoliata, PsPK2, PsPIN1 and LE gene expression in pea (Pisum sativum) shoot tips. Plant Cell Physiol. 47: 935-948. Baniwal, S. K., K. Bharti, K. Y. Chan, M. Fauth, A. Ganguli, S. Kotak, S. K. Mishar, L. Nover, M. Port, K.-D. Scharf, J. Tripp, C. Weber, D. Zielinski, and P. von Koskull-Döring. 2004. Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors. J. Biosci. 29: 471-487. Barnett, T., M. Altschuler, C. N. McDaniel, and J. P. Mascarenhas. 1980. Heat shock induced proteins in plant cells. Dev. Gen. 1: 331-340. Bechtold, N., J. Ellis, and G. Pelletier. 1993. In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C. R. Acad. Sci. Paris, Life Sci. 316: 1194-1199. Bösl, B., V. Grimminger, and S. Walter. 2006. The molecular chaperone Hsp104—a molecular machine for protein disaggregation. J. Structural Biol. 156: 139-148. Bukau, B. and A. L. Horwich. 1998. The Hsp70 and Hsp60 chaperone machines. Cell 92: 351-366. Busk, P. K. and M. Pagès. 1998. Regulation of abscisic acidinduced transcription. Plant Mol. Biol. 37: 425-435. Chan, A. P., J. Crabtree, Q. Zhao, H. Lorenzi, J. Orvis, D. Puiu, A. Melake-Berhan, K. M. Jones, J. Redman, G. Chen, E. B. Cahoon, M. Gedil, M. Stanke, B. J. Haas, J. R. Wortman, C. M. Fraser-Liggett, J. Ravel, and P. D. Rabinowicz. 2010. Draft genome sequence of the ricin-producing oilseed castor bean. Nat Biotechnol. 28: 951-956. Cheetham, M. E. and A. J. Caplan. 1998. Structure, function and evolution of DnaJ: conservation and adaptation of chaperone function. Cell Stress Chaperones 3: 28-36. Choi, H. I., J.-H. Hong, J.-O. Ha, J.-Y. Kang, and S. Y. Kim. 2000. ABFs, a Family of ABA-responsive element binding factors. J. Biol. Chem. 275: 1723-1730. Cour, T. L., R. Gupta, K. Rapacki, K. Skriver, F. M. Poulsen, and S. Brunak. 2003. NESbase version 1.0: a database of nuclear export signals. Nucleic Acids Res. 31: 393-396. Craig, E. A., P. Huang, R. Aron, and A. Andrew. 2006. The diverse roles of J-proteins, the obligate Hsp70 co-chaperone. Rev. Physiol. Biochem. Pharmacol. 156: 1-21. Dellaporta, S. L., J. Wood, and J. B. Hicks. 1983. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1: 19-21. Doyle, S. M. and S. Wickner. 2008. Hsp104 and ClpB: protein disaggregating machines. Trends Biochemical. Sci. 34: 40-48. Ellard-Ivey, M. and C. J. Douglas. 1996. Role of jasmonates in the elicitor- and wound-inducible expression of defense genes in parsley and transgenic tobacco. Plant Physiol. 112: 183-192. Elizabeth, E. H., G. L. Helmer, R. T. Fraley, and M.-D. Chilton. 1986. The Hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J. Bacteriol. 168: 1291-1301. Feinberg, A. P. and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6-13. Fink, A. L. 1999. Chaperone-mediated protein folding. Physiol. Rev. 79: 424-449. Ham, B.-K., J. M. Park, S.-B. Lee, M. J. Kim, I.-J. Lee, K.-J. Kim, C. S. Kwon, and K.-H. Paek. 2006. Tobacco Tsip1, a DnaJ-type Zn finger protein, is recruited to and potentiates Tsi1-mediated transcriptional activation. Plant Cell 18: 2005-2020. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmid. J. Mol. Biol. 166: 557-580. Haslbeck, M. 2002. sHsps and their role in the chaperone network. Cell. Mol. Life Sci. 59: 1649-1657. Hoekema, A., P. R. Hirsch, P. J. J. Hooykaas, and R. A. Schilperoort. 1983. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumerfaciens Ti plasmid. Nature 303: 179-180. Hofagen, R. and L. Willmitzer. 1988. Storage of cpmpetent cells for Agrobacterium transformation. Nucleuc Acids Res. 15: 9877. Horsch, R. B., J. E. Fry, N. L. Hoffmann, D. Erichholtz, S. G. Rogers, and R. T. Fraley. 1985. A simple and general method for transferring genes into plants. Science 227: 1229-1231. Hu, W.-W., S.-M. Wong, C.-S. Loh, and C.-J. Goh. 1998. Synergism in replication of cymbidium mosaic potexvirus (CymMV) and odontoglossum ringspot tobamovirus (ORSV) RNA in orchid protoplasts. Arch. Virol. 143: 1265-1275. Jayaraman, K., S. A. Fingar, J. Shah, and J. Fyles. 1991. Polymerase chain reaction-mediated gene synthesis: synthesis of a gene coding for isozyme c of horseradish peroxidase. Proc. Natl. Acad. Sci. USA 88: 4084-4088. Jefferson, R. A. 1987. Assaying chimeric genes in plant: the GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387-405. Key, J. L., C. Y. Lin, and Y. M. Chen. 1981. Heat shock proteins of higher plants. Proc. Natl. Acad. Sci. USA 78: 3526-3530. Kim, S., J.-Y. Kang, D.-I. Cho, J. H. Park, and S. Y. Kim. 2004. ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance. Plant J. 40: 75-87. Klein, M., E. D. Wolf, R. Wu, and J. C. Sanford. 1987. High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70-73. Kneissl, J., V. Wachtler, N.-H. Chua, and C. Bollea. 2009. OWL1: an Arabidopsis J-domain protein involved in perception of very low light fluences. Plant Cell 21: 3212-3225. Kobayashi, M., H. Katoh, T. Takayanagi, and S. Suzuki. 2010. Characterization of thermotolerance-related genes in grapevine (Vitis vinifera). J. Plant Physiol. 167: 812-819. Kotak, S., J. Larkindale, U. Lee, P. von Koskull-Döring, E. Vierling, and K. -D. Scharf. 2007. Complexity of the heat stress response in plants. Curr. Opin. Plant Biol. 10: 310-316. Kusmierczyk, A. R. and J. Martin. 2001. Chaperonins - keeping a lid on folding proteins. FEBS Let. 505: 343-347. Lee, S., M. E. Sowa, Y.-H. Watanabe, P. B. Sigler, W. Chiu, M. Yoshida, and F. T.F. Tsai. 2003. The structure of ClpB: a molecular chaperone that rescues proteins from an aggregated state. Cell 115: 229-240. Li, J., X. Qian, and B. Sha. 2003. The crystal structure of the yeast Hsp40 Ydj1 complexed with its peptide substrate. Structure 11: 1475-1483. Li, J., X. Qian, and B. Sha. 2009. Heat shock protein 40: structural studies and their functional implications. Protein Pept. Lett. 16: 606-612. Liscum, E. and J. W. Reed. 2002. Genetics of Aux/IAA and ARF action in plant growth and development. Plant Mol. Biol. 49: 387-400. Lu, Z. and D. M. Cyr. 1998. The conserved carboxyl terminus and zinc finger-like domain of the co-chaperone Ydj1 assist Hsp70 in protein folding. J. Biol. Chem. 273: 5970-5978. Makkerh, J. P. S., C. Dingwall and R. A. Laskey. 1996. Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acids. Curr. Biol. 6:1025-1027. Martin, J. and F. U. Hartl. 1993. Protein folding in the cell: molecular chaperones pave the way. Structure 1: 161-164. Martinez-Yamout, M., G. B. Legge, O. Zhang, P. E. Wright, and H. J. Dyson. 2000. Solution structure of the cysteine-rich domain of the Escherichia coli chaperone protein DnaJ. J. Mol. Biol. 300: 805-818. Menke, F. L. H., A. Champion, J. W. Kijne, and J. Memelink. 1999. A novel jasmonate- and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene Str interacts with a jasmonate- and elicitor-inducible AP2-domain transcription factor, ORCA2. EMBO J. 18: 4455-4463. Morimoto, R. 1993. Cell in stress: transcriptional activation of heat shock genes. Science 259: 1409-1410. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Planta. 15: 473-497. Perez, D. E., J. S. Hoyer, A. I. Johnson, Z. R. Moody, J. Lopez, and N. J. Kaplinsky. 2009. BOBBER1 is a noncanonical arabidopsis small heat shock protein required for both development and thermotolerance. Plant Physiol. 151: 241-252. Picard, D. 2002. Heat-shock protein 90, a chaperone for folding and regulation. Cell. Mol. Life Sci. 59: 1640-1648. Prodromou, C., S. M. Roe, R. O'Brien, J. E. Ladbury, P. W. Piper, and L. H. Pearl. 1997. Identification and structural characterization of the ATP/ADP binding site in the hsp90 molecular chaperone. Cell 90: 65-75. Queitsch, C., S. -W. Hong, E. Vierling, and S. Lindquist. 2000. Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis. Plant Cell 12: 479-492. Rajan, V.B.V. and P. D’Silva. 2009. Arabidopsis thaliana J-class heat shock proteins: cellular stress sensors. Funct. Integr. Genomics 9: 433-446. Ritossa, F. 1962. A New Puffing Pattern Induced by Temperature Shock and DNP in Drosophila. Experientia 18: 571-573. Rouster, J., R. Leah, J. Mundy, and V. Cameron-Mills. 1997. Identification of a methyl jasmonate-responsive region in the promoter of a lipoxygenase 1 gene expressed in barley grain. Plant J. 11: 513-523. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd edition. Cold Spring Harbor Laboratory Press, New York. Sato, Y. and S. Yokoya. 2008. Enhanced tolerance to drought stress in transgenic rice plants overexpressing a small heat-shock protein, sHSP17.7. Plant Cell Rep. 27: 329-334. Shen, L., Y. G. G. Kang, L. Liu, and H. Yu. 2011. The J-domain protein J3 mediates the integration of flowering signals in Arabidopsis. Plant Cell 23: 499-514. Shen, W. J. and B. G. Forde. 1989. Efficient transformation of Agrobacterium spp. By high voltage electroporation. Nucleic Acids Res. 17: 8385. Shim, D., J.-U. Hwang, J. Lee, S. Lee, Y. Choi, G. An, E. Martinoia, and Y. Lee. 2009. Orthologs of the class A4 heat shock transcription factor HsfA4a confer cadmium tolerance in wheat and rice. Plant Cell 21: 4031-4043. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503-517. Sun, W., C. Bernard, B. van de Cotte, M. V. Montagu, and N. Verbruggen. 2001. At-HSP17.6A, encoding a small heat-shock protein in Arabidopsis, can enhance osmotolerance upon overexpression. Plant J. 27: 407-415. Takabe, T., A. Uchida, F. Shinagawa, Y. Terada, H. Kajita, Y. Tanaka, T. Takabe, T. Hayashi, T. Kawai, and T. Takabe et al. 2008. Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants. Plant Growth Regul. 56:265-273. Tanaka, T., B. A. Antonio, S.Kikuchi, T. Matsumoto, Y. Nagamura, H. Numa, H. Sakai, J. Wu, T. Itoh, T. Sasaki. 2008. The Rice Annotation Project Database (RAP-DB): 2008 update. Nucleic Acids Res. 36: 1028-1033. Theologis, A., J. R. Ecker, C. J. Palmk, N. A. Federspiel, S. Kaul, O. White, J. Alonso, H. Altafi, R. Araujok, C. L. Bowman et al. 2000. Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana. Nature 408: 816-820. Tissieres, A., H. K. Mitchell, and U. M. Tracy. 1974. Protein synthesis in salivary glands of Drosophila melanogaster: relation to chromosome puffs. J. Mol. Biol. 84: 389-398. Tsai, J. and M. G. Douglas. 1996. A conserved HPD sequence of the J-domain Is necessary for YDJ1 stimulation of Hsp70 ATPase activity at a site distinct from substrate binding. J. Biol. Chem. 271: 9347-9354. Tsuchisak, A. and A. Theologis. 2004. Unique and overlapping expression patterns among the Arabidopsis 1-amino-cyclopropane-1-carboxylate synthase gene family members. Plant Physiol. 136: 2982-3000. Tuskan, G. A., S. DiFazio, S. Jansson, J. Bohlmann, I. Grigoriev, U. Hellsten, N. Putnam, S. Ralph, S. Rombauts, A. Salamov et al. 2006. The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray). Science 313: 1596-1604. Ulmasov, T., Z.-B. Liu, G. Hagen, and T. J. Guilfoyle. 1995. Composite structure of auxin response elements. Plant Cell 7: 1611-1623. Urao, T., K. Yamaguchi-Shinozaki, S. Urao, and K. Shinozaki. 1993. An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence. Plant Cell 5: 1529-1539. Vierling, E. 1991. The roles of heat shock proteins in plants. Annu. Rev. Physiol. Plant Mol. Biol. 42: 579-620. Vinocur, B. and A. Altman. 2005. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr. Opin. Biotechnol. 16: 123-132. Wang, H., B. Jones, Z. Li., P. Frasse, C. Delalande, F. Regad, S. Chaabouni, A. Latché, J.-C. Pech, and M. Bouzayena. 2005. The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis. Plant Cell 17: 2676-2692. Wang, W., B. Vinocur, O. Shoseyov, and A. Altman. 2004. Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci. 9: 244-252. William B. T. and A. R. Cashmore. 1995. Light-regulated transcription. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46: 445-74. Xiao, H. and J. T. Lis. 1988. Germline transformation used to define key features of heat-shock response elements. Sci. 239: 1139-1142. Xu, X., H. Song, Z. Zhou, N. Shi, Q. Ying, and H. Wang. 2010. Functional characterization of AtHsp90.3 in Saccharomyces cerevisiae and Arabidopsis thaliana under heat stress. Biotechnol. Lett. 32: 979-987. Yamada, K. and M. Nishimura. 2008. Cytosolic heat shock protein 90 regulates heat shock transcription factor in Arabidopsis thaliana. Plant Signaling Behavior 3: 660-662. Yabe, N., T. Takahashi, and Y. Komed. 1994. Analysis of tissue-specific expression of Arabidopsis thaliana HSP90-family gene HSP81. Plant Cell Physiol. 35: 1207-1219. Yang, J.-Y., Y. Sun, and A.-Q. Sun. 2006. The involvement of chloroplast HSP/ClpB in the acquired thermotolerance in tomato. Plant Mol. Biol. 62: 385-395. Yoo, S.-D., Y.-H. Cho, and J. Sheen. 2007. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nature Protocols 2: 1565-1572. Zahur, M., A. Maqbool, M.Irfan, M. Y. K. Barozai, U. Qaiser, B. Rashid, T. Husnain, and S. Riazuddin. 2009. Functional analysis of cotton small heat shock protein promoter region in response to abiotic stresses in tobacco using Agrobacterium-mediated transient assay. Mol. Biol. Rep. 36: 1915-1921. Zhang, X., Y. H. Hu, C. H. Jiang, W. Z. Zhang, Z. Li, and F. Ming. 2011. Isolation of the Chinese rose sHSP gene promoter and its differential regulation analysis in transgenic Arabidopsis plants. Mol. Biol. Rep. DOI 10.1007/s11033-011-0843-x. Zhao, Z., W. Zhang, J. Yan, J. Zhang, Z. Liu, X. Li and Y. Yi. 2010. Over-expression of Arabidopsis DnaJ (Hsp40) contributes to NaCl-stress tolerance. African J. Biotechnol. 9: 972-978. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9769 | - |
| dc.description.abstract | 香蕉 (Musa spp.) 為大型單子葉草本植物，是臺灣重要的經濟作物。本研究針對香蕉果實後熟相關MhDnaJ基因，進行過量表達 (overexpression)、基因默化 (RNA interference, RNAi)、蛋白質定位 (protein localization) 及啟動子活性分析 (promoter activity analysis)，以瞭解香蕉果實後熟相關蛋白質MhDnaJ之功能及其啟動子之表現特性。蛋白質定位質體以2 X CaMV 35S啟動子驅動MhDnaJ融合綠色螢光蛋白 (green fluorescent protein, GFP) ，分別以阿拉伯芥原生質體轉殖與基因槍法轉殖至洋蔥表皮細胞，進行暫時性表現分析，以共軛焦顯微鏡觀察，MhDnaJ::GFP融合蛋白於細胞核內表達。MhDnaJ啟動子引導報導基因GUS之菸草轉殖植株，經南方氏雜交分析確認均為轉殖株，於T1轉殖株播種後5-30天啟動子皆無表現，進一步分析菸草R1轉殖株花序與果實不同生長發育階段之啟動子活性，顯示MhDnaJ啟動子於柱頭發育早期 (stages 2-4) 與後期 (stage 8)、雄蕊發育後期 (stages 8-9) 與果實發育早期 (stages 10-11) 表現，顯示啟動子活性於花器表現。不同非生物性逆境與生長調節劑誘導處理，皆無法誘導啟動子表現。 | zh_TW |
| dc.description.abstract | Banana (Musa spp.), the largest herbaceous monocot flowering plant, is an important crop in Taiwan. This research aims to understand gene function of banana ripening-associated gene MhDnaJ by employing gene overexpression studies and gene silencing through RNA interference strategy. Furthermore, the localization of MhDnaJ protein and promoter activity were studied. MhDnaJ cDNA was fused with green fluorescence protein gene driven by 2 X CaMV 35S promoter, transformed to Arabidopsis protoplasts and onion epidermal cells, then analyzed by confocal fluorescence microscopy for protein localization. The MhDnaJ protein was found to be localized in the nucleus as evidenced by green fluorescence. Twelve putative transgenic tobacco plants containing promoter construct were confirmed by Southern blot analysis. No expression of MhDnaJ promoter was detected in 5 to 30 day-old plants, but was found to be expressed in flower (pistil: stages 2-4, 8; stamen: stages 8-9) and fruit (stages 10-11) as detected by GUS histochemical staining. None of the abiotic stresses or any plant growth regulators could induce MhDnaJ promoter activity. | en |
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| dc.description.tableofcontents | 中文摘要...................................................I
Abstract..................................................II 壹、前言...................................................1 貳、前人研究...............................................2 一、熱休克蛋白質...........................................2 二、熱休克蛋白質之種類與特性...............................3 三、植物熱休克蛋白質之作用機制.............................5 四、植物熱休克蛋白質之功能分析.............................6 五、熱休克蛋白質Hsp40 (DnaJ /J-protein)...................10 參、材料與方法............................................13 一、試驗材料..............................................13 (一) 菌種材料.............................................13 (二) 植物材料.............................................13 (三) 質體材料.............................................13 二、試驗方法..............................................17 (一) 基因啟動子與胺基酸序列構分析.........................17 1. 基因啟動子序列分析.....................................17 2. 胺基酸序列分析.........................................17 (二) 轉殖質體之構築.......................................20 1. 質體DNA之小量製備......................................20 2. DNA片段回收............................................20 3. 連接反應...............................................20 4. 大腸桿菌勝任細胞之製備.................................21 5. 轉型反應...............................................21 6. 聚合酶連鎖反應.........................................22 7. DNA 5’ 末端突出補平反應...............................22 8. DNA 3’ 末端突出切平反應...............................22 9. DNA定序................................................23 (三) 質體DNA之大量製備....................................23 (四) 阿拉伯芥原生質體轉殖.................................24 1. 原生質體之抽取.........................................24 2. 原生質體之轉殖.........................................24 (五) 基因槍法暫時性表現...................................25 1. 微粒子製備與DNA包裹....................................25 2. 洋蔥表皮細胞之基因轉殖.................................25 (六) 農桿菌轉型...........................................26 1. 農桿菌勝任細胞之製備...................................26 2. 電穿孔轉型反應.........................................26 3. 農桿菌質體 DNA 之小量製備..............................27 (七) 阿拉伯芥基因轉殖及轉殖株篩選.........................28 1. 阿拉伯芥花序浸潤轉殖法.................................28 2. 轉殖株之篩選...........................................29 (八) 菸草基因轉殖及轉殖株篩選.............................29 1. 菸草葉圓片轉殖法.......................................29 2. 轉殖株之篩選與再生.....................................30 (九) 擬轉殖株之分析.......................................30 1. 基因組DNA之抽取........................................30 2. 樣品製備...............................................31 3. 探針製備...............................................31 4. 南方氏雜交分析.........................................32 5. GUS活性組織化學染色法..................................32 (十) 轉殖株之啟動子活性與誘導分析.........................33 1. 無菌播種...............................................33 2. 不同發育階段之啟動子活性分析...........................33 3. 不同非生物性逆境誘導處理...............................33 4. 不同生長調節劑誘導處理.................................34 肆、結果..................................................36 一、香蕉MhDnaJ基因結構分析................................36 (一) 香蕉MhDnaJ基因序列分析...............................36 (二) 香蕉MhDnaJ基因啟動子序列分析.........................36 二、香蕉MhDnaJ基因功能分析................................46 三、香蕉MhDnaJ蛋白質定位分析..............................46 四、香蕉MhDnaJ基因啟動子活性分析..........................49 (一) 菸草轉殖株基因完整性與基因拷貝數之分子驗證...........49 (二) 菸草轉殖株不同發育階段之啟動子活性分析...............49 (三) 不同非生物性逆境與生長調節劑誘導之菸草轉殖株啟動子活性分析......................................................55 伍、討論..................................................61 一、香蕉MhDnaJ基因結構分析................................61 (一) 香蕉MhDnaJ基因序列分析...............................61 (二) 香蕉MhDnaJ基因啟動子序列分析.........................63 二、香蕉MhDnaJ蛋白質定位分析..............................65 三、香蕉MhDnaJ啟動子活性分析..............................67 陸、結語..................................................68 柒、參考文獻..............................................69 | |
| dc.language.iso | zh-TW | |
| dc.title | 香蕉果實後熟相關基因MhDnaJ之啟動子活性與基因功能分析 | zh_TW |
| dc.title | Analysis of Promoter Activity and Gene Function on the Fruit Ripening-associated Gene MhDnaJ from Banana | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 杜宜殷(Yi-Yin Do) | |
| dc.contributor.oralexamcommittee | 黃銓珍(Chang-Jen Huang),洪傳揚(Chwan-Yang Hong) | |
| dc.subject.keyword | 香蕉,熱休克蛋白質,J 蛋白質,阿拉伯芥原生質體,啟動子活性分析, | zh_TW |
| dc.subject.keyword | Banana,Heat shock protein,J-protein,Arabidopsis protoplast,promoter acativity anaysis, | en |
| dc.relation.page | 77 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2011-08-09 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝學研究所 | zh_TW |
| 顯示於系所單位： | 園藝暨景觀學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-100-1.pdf | 14.79 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。