請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9727
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭石通 | |
dc.contributor.author | Jeng-Shane Lin | en |
dc.contributor.author | 林振祥 | zh_TW |
dc.date.accessioned | 2021-05-20T20:37:52Z | - |
dc.date.available | 2013-08-05 | |
dc.date.available | 2021-05-20T20:37:52Z | - |
dc.date.copyright | 2008-08-05 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-25 | |
dc.identifier.citation | Allen, E., Xie, Z., Gustafson, A.M., and Carrington, J.C. (2005). microRNA-directed phasing during trans-acting siRNA biogenesis in plants. Cell 121: 207-221.
Alvarez, M.E., Pennell, R.I., Meijer, P.J., Ishikawa, A., Dixon, R.A., and Lamb, C. (1998). Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell 92: 773-784. Apostol, I., Heinstein, P.F., and Low, P.S. (1989). Rapid stimulation of an oxidative burst during elicidation of cultured plant cells. Role in defense and signal transduction. Plant Physiol. 90: 106-116. Aukerman, M.J., and Sakai, H. (2003). Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes. Plant Cell 15: 2730-2741. Axtell, M.J., Snyder, J.A., and Bartel, D.P. (2007). Common functions for diverse small RNAs of land plants. Plant Cell 19: 1750-1769. Bartel, B. (2005). MicroRNAs directing siRNA biogenesis. Nat. Struct. Mol. Biol. 12: 569-571. Bartel, D.P. (2004). MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116: 281-297. Bergey, D.R., Howe, G.A., and Ryan, C.A. (1996). Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals. Proc. Natl. Acad. Sci. USA 93: 12053-12058. Bergey, D.R., and Ryan, C.A. (1999). Wound- and systemin-inducible calmodulin gene expression in tomato leaves. Plant Mol. Biol. 40: 815-823. Birkenmeier, G.F., and Ryan, C.A. (1998). Wound signaling in tomato plants. Evidence that aba is not a primary signal for defense gene activation. Plant Physiol. 117: 687-693. Carrington, J.C., and Ambros, V. (2003). Role of microRNAs in plant and animal development. Science 301: 336-338. Chen, X. (2004). A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development. Science 303: 2022-2025. Chiou, T.J., Aung, K., Lin, S.I., Wu, C.C., Chiang, S.F., and Su, C.L. (2006). Regulation of phosphate homeostasis by MicroRNA in Arabidopsis. Plant Cell 18: 412-421. Combier, J.P., Frugier, F., de Billy, F., Boualem, A., El-Yahyaoui, F., Moreau, S., Vernié, T., Ott, T., Gamas, P., Crespi, M., and Niebel, A. (2006). MtHAP2-1 is a key transcriptional regulator of symbiotic nodule development regulated by microRNA169 in Medicago truncatula. Genes Dev. 20: 3084-3088. Creelman, R.A., and Mullet, J.E. (1995). Jasmonic acid distribution and action in plants: regulation during development and response to biotic and abiotic stress. Proc. Natl. Acad. Sci. USA 92: 4114-4119. Dekker, J., and Hargrove, M. (2002). Weedy adaptation in Setaria spp. V. Effects of gaseous environment on giant foxtail (Setaria faberii) (Poaceae) seed germination. Am. J. Bot. 89: 410-416. Delledonne, M., Xia, Y., Dixon, R.A., and Lamb, C. (1998). Nitric oxide functions as a signal in plant disease resistance. Nature 394: 585-588. Doench, J.G., and Sharp, P.A. (2004). Specificity of microRNA target selection in translational repression. Genes Dev. 18: 504-511. Enslen, H., Tokumitsu, H., Stork, P.J.S., Davis, R.J., and Soderling, T.R. (1996). Regulation of mitogen-activated protein kinases by a calcium/calmodulin-dependent proteins kinase cascade. Proc. Natl. Acad. Sci. USA 93: 10803-10808. Farmer, E.E., and Ryan, C.A. (1992). Octadecanoid Precursors of Jasmonic Acid Activate the Synthesis of Wound-I.ducible Proteinase Inhibitors. Plant Cell 4: 129-134. Fujii, H., Chiou, T.J., Lin, S.I., Aung, K., and Zhu, J.K. (2005). A miRNA involved in phosphate-starvation response in Arabidopsis. Curr. Biol. 15: 2038-2043. Hammond, S.C., Bernstein, E., Beach, D., and Hannon, G.J. (2000). An RNA-directed nuclease mediates posttranscriptional gene silencing in Drosophila cells. Nature 404: 293-296. Han, Y., Zhang, J., Chen, X., Gao, Z., Xuan, W., Xu, S., Ding, X., and Shen, W. (2008). Carbon monoxide alleviates cadmium-induced oxidative damage by modulating glutathione metabolism in the roots of Medicago sativa. New Phytol. 177: 155-166. Jih, P.J., Chen, Y.C., and Jeng, S.T. (2003). Involvement of hydrogen peroxide and nitric oxide in expression of the ipomoelin gene from sweet potato. Plant Physiol. 132: 381-389. Kandoth, P.K., Ranf, S., Pancholi, S.S., Jayanty, S., Walla, M.D., Miller, W., Howe, G.A., Lincoln, D.E., and Stratmann, J.W. (2007). Tomato MAPKs LeMPK1, LeMPK2, and LeMPK3 function in the systemin-mediated defense response against herbivorous insects. Proc. Natl. Acad. Sci. USA 104: 12205-12210. Kende, H. (1993). Ethylene biosynthesis. Annu. Rev. plant physiol. 44: 283-307. Knight, H., Trewavas, A.J., and Knight, M.R. (1996). Cold calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after acclimation. Plant Cell 8: 489-503. Knight, M.R., Smith, S.M., and Trewavas, A.J. (1992). Wind-induced plant motion immediately increases cytosolic calcium. Proc. Natl. Acad. Sci. USA 89: 4967-4971. Kolomiets, M.V., Hannapel, D.J., Chen, H., Tymeson, M., and Gladon, R.J. (2001). Lipoxygenase is involved in the control of potato tuber development. Plant Cell 13: 613-626. Kurihara, Y., and Watanabe, Y. (2004). Arabidopsis micro-RNA biogenesis through Dicer-like 1 protein functions. Proc. Natl. Acad. Sci. USA. 101: 12753-12758. Lamb, C., and Dixon, R.A. (1997). The oxidative burst in plant disease resistance. Annu. Rev. Plant Physiol. Plant Mol. Bio. 48: 251-275. Lee, Y., Kim, M., Han, J., Yeom, K.H., Lee, S., Baek, S.H., and Kim, V.N. (2004). MicroRNA genes are transcribed by RNA polymerase II. EMBO J. 23: 4051-4060. Lincoln, J.E., Campbell, A.D., Oetiker, J., Rottmann, W.H., Oeller, P.W., Shen, N.F., and Theologis, A. (1993). LE-ACS4, a fruit ripening and wound-induced 1-aminocyclopropane-1-carboxylate synthase gene of tomato (Lycopersicon esculentum). Expression in Escherichia coli, structural characterization, expression characteristics, and phylogenetic analysis. J. Biol. Chem. 268: 19422-19430. Liu, K., Xu, S., Xuan, W., Ling, T., Cao, Z., Huang, B., Sun, Y., Fang, L., Liu, Z., Zhao, N., and Shen, W. (2007). Carbon monoxide counteracts the inhibition of seed germination and alleviates oxidative damage caused by salt stress in Oryza sativa. Plant Sci. 172: 544-555. Lu, C., Meyers, B.C., and Green, P.J. (2007). Construction of small RNA cDNA libraries for deep sequencing. Methods 43:110-117. Lund, E., Guttinger, S., Calado, A., Dahlberg, J.E., and Kutay, U. (2004). Nuclear export of microRNA precursors. Science 303: 95-98. Mallory, A.C., Reinhart, B.J., Jones-Rhoades, M.W., Tang, G., Zamore, P.D., Barton, M.K., and Bartel, D.P. (2004). MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5' region. EMBO J. 23: 3356-3364. Mallory, A.C., Bartel, D.P., Bartel, B. (2005). MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes. Plant Cell. 17: 1360-1375. Memelink, J., Verpoorte, R., and Kijne, J.W. (2001). Organization of jasmonate-responsive gene expression in alkaloid metabolism. Trends in Plant Science 6: 212-219. Martinez, J., Patkaniowska, A., Urlaub, H., Luhrmann, R., and Tuschl, T. (2002). Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. Cell 110: 563-574. Muramoto, T., Tsurui, N., Terry, M.J., Yokota, A., and Kohchi, T. (2002). Expression and biochemical properties of a ferredoxin-dependent heme oxygenase required for phytochrome chromophore synthesis. Plant Physiol. 130: 1958-1966. Navarro, L., Dunoyer, P., Jay, F., Arnold, B., Dharmasiri, N., Estelle, M., Voinnet, O., and Jones, J.D. (2006). A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science. 312: 436-439. Noriega, G.O., Balestrasse, K.B., Batlle, A.M.C., and Tomaro, M.L. (2004). Heme oxygenase exerts a protective role against oxidative stress in soybean leaves. Biochem. Biophys. Res. Commun. 3223: 1003-1008. O'Donnell, P.J., Calvert, C., Atzorn, R., Wasternack, C., Leyser, H.M.O., and Bowles, D.J. (1996). Ethylene as a signal mediating the wound response of tomato plants. Science 274: 1914-1917. Olson DC, White JA, Edelman L, Harkins RN, and Kende H. (1991). Differential expression of two genes for 1-aminocyclopropane-1-carboxylate synthase in tomato fruits. Proc. Natl. Acad. Sci. USA 88: 5340-5344. Orozco-Cárdenas, M.L., and Ryan, C.A. (1999). Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway. Proc. Natl. Acad. Sci. USA 96: 6553-6557. Park, M.Y., Wu, G., Gonzalez-Sulser, A., Vaucheret, H., and Poethig, R.S. (2005). Nuclear processing and export of microRNAs in Arabidopsis. Proc. Natl. Acad. Sci. USA 102: 3691-3696. Peña-Cortés, H., Fisahn, J., and Willmitzer, L. (1995). Signals involved in wound-induced proteinase inhibitor II gene expression in tomato and potato plants. Proc. Natl. Acad. Sci. USA 92: 4106-4113. Qui, Z.-H., Gijon, M.A., de Carvalho, M.S., Spencer, D.M., and Leslie, C.C. (1998) The role of calcium and phosphorylation of cytosolic phospholipase A2 in regulating arachidonic acid release in macrophages. J. Biol. Chem. 273: 8203-8211. Rajagopalan, R., Vaucheret,H., Trejo, J., and Bartel, D.P. (2006). A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana. Genes Dev. 20: 3407-3425. Reinbothe, S., Mollenhauer, B., and Reinbothe, C. (1994). JIPs and RIPs: the regulation of plant gene expression by jasmonates in response to environmental cues and pathogens. Plant Cell 6: 1197-1209. Rhoades, M.W., Reinhart, B.J., Lim, L.P., Burge, C.B., Bartel, B., and Bartel, D.P. (2002). Prediction of plant microRNA targets. Cell 110: 513-520. Reyes, J.L., and Chua, N.H. (2007). ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination. Plant J. 49: 592-606. Ryan, C.A. (2000).The systemin signaling pathway: differential activation of plant defensive genes. Biochim. Biophys. Acta. 1477: 112-121. Reymond, P., Weber, H., Damond, M., and Farmer, E.E. (2000). Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. Plant Cell 12: 707-720. Schaller, F. (2001). Enzymes of the biosynthesis of octadecanoid-derived signalling molecules. J. Exp. Bot. 52: 11-23. Schilmiller, A.L., and Howe, G.A. (2005). Systemic signaling in the wound response. Curr. Opin. Plant Biol. 8: 369-377. Schwarz, D.S., Hutvágner, G., Haley, B., and Zamore, P.D. (2002). Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways. Mol. Cell 10: 537-548. Sheen, J. (1996) Ca2+-dependent protein kinases and stress signal transduction in plants. Science 274: 1900-1902. Shiu, O.Y., Oetiker, J.H., Yip, W.K., and Yang, S.F. (1989). The promoter of LE-ACS7, an early flooding-induced 1-aminocyclopropane-1-carboxylate synthase gene of the tomato, is tagged by a Sol3 transposon. Proc. Natl. Acad. Sci. USA 95: 10334-10339. Song, X.G., She, X.P., and Zhang, B. (2008). Carbon monoxide-induced stomatal closure in Vicia faba is dependent on nitric oxide synthesis. Physiol. Plant 132: 514-525. Sunkar, R., Chinnusamy, V., Zhu, J., and Zhu, J.K. (2007). Small RNAs as big players in plant abiotic stress responses and nutrient deprivation. Trends Plant Sci. 12: 301-309. Sunkar, R., Kapoor, A., and Zhu, J.K. (2006). Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by downregulation of miR398 and important for oxidative stress tolerance. Plant Cell 18: 2051-2065. Sunkar, R., and Zhu, J.K. (2004). Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis. Plant Cell 16: 2001-2019. Talmor-Neiman, M., Stav, R., Klipcan, L., Buxdorf, K., Baulcombe, D.C., and Arazi, T. (2006). Identification of trans-acting siRNAs in moss and an RNA-dependent RNA polymerase required for their biogenesis. Plant J. 48: 511-521. Tang,G., Reinhart, B.J., Bartel, D.P., and Zamore, P.D. (2003). A biochemical framework for RNA silencing in plants. Genes Dev. 17: 49-63. Tsuji, H., Aya, K., Ueguchi-Tanaka, M., Shimada, Y., Nakazono, M., Watanabe, R., Nishizawa, N.K., Gomi, K., Shimada, A., Kitano, H., Ashikari, M., and Matsuoka, M. (2006). GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers. Plant J. 47: 427-444. Vaucheret, H., Vazquez, F., Crete, P., Bartel, D.P. (2004). The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development. Genes Dev. 18: 1187-1197. Walling, L.L. (2000). The myriad plant responses to herbivores. J. Plant Growth Regul. 19: 195-216. Watanabe, T., and Sakai, S. (1998). Effects of active oxygen species and methyl jasmonate on expression of the gene for a woundinducible 1-aminocyclopropane-1-carboxylate synthase in winter squash (Cucurbita maxima). Planta 206: 570-576. Wendehenne, D., Durner, J., and Klessig, D.F. (2004). Nitric oxide: a new player in plant signalling and defence responses. Curr. Opin. Plant Biol. 7: 449-455. Wu, M.F., Tian, Q., and Reed, J.W. (2006). Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction. Development. 133: 4211-4218. Xie, Y., Ling, T., Han, Y., Liu, K., Zheng, Q., Huang, L., Yuan, X., He, Z., Hu, B., Fang, L., Shen, Z., Yang, Q., and Shen, W. (2008). Carbon monoxide enhances salt tolerance by nitric oxide-mediated maintenance of ion homeostasis and up-regulation of antioxidant defense in wheat seedling roots. Plant Cell Environ. [Epub ahead of print] Xie, Z., Kasschau, K.D., Carrington, J.C. (2003). Negative feedback regulation of Dicer-Like1 in Arabidopsis by microRNA-guided mRNA degradation. Curr. Biol. 13: 784-789. Yang, J.H., Han, S.J., Yoon, E.K., and Lee, W.S. (2006). Evidence of an auxin signal pathway, microRNA167-ARF8-GH3, and its response to exogenous auxin in cultured rice cells. Nucleic Acids Res. 34: 1892-1899. Yan, K.S., Yan, S., Farooq, A., Han, A., Zeng, L., and Zhou, M.M. (2003). Structure and conserved RNA binding of the PAZ domain. Nature 426: 468-474. Yoo, B.C., Kragler, F., Varkonyi-Gasic, E., Haywood, V., Archer-Evans, S., Lee, Y.M., Lough, T.J., and Lucas, W.J. (2004). A systemic small RNA signaling system in plants. Plant Cell 16: 1979-2000. Yu, B., Yang, Z., Li, J., Minakhina, S., Yang, M., Padgett, R.W., Steward, R., and Chen, X. (2005). Methylation as a crucial step in plant microRNA biogenesis. Science 307: 932-935. Zeng, Y., and Cullen, B.R. (2004). Structural requirements for pre-microRNA binding and nuclear export by Exportin 5. Nucleic Acids Res. 32: 4776-4785. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9727 | - |
dc.description.abstract | microRNA (miRNA)是一段由生物體內自行合成約21-24鹼基的小分子核醣核酸,不轉譯出任何蛋白,其藉由降解mRNA或抑制mRNA進行蛋白質轉譯來達成調控基因表現。近年來在阿拉伯芥上發現超過百種的miRNA,且研究指出miRNA參與植物不同階段的生長發育及抵抗不同環境的變化,所以我們對於與植物傷害相關的miRNA有著極大興趣,希望探討傷害相關miRNA的訊息傳遞的過程。藉由miRNA序列在演化具有高度保留性的特性,利用阿拉伯芥miRNA的序列當作探針探討甘藷植株在遭受傷害後miR159、miR407、miR828的表現情形。在甘藷植株中無偵測到miR407的表現;miR159可在莖中被偵測到,而在葉中表現量極低;miR828則在葉中被偵測到。此外,在甘藷傷害後,miR159表現量並未隨時間不同有所變化,而miR828表現量卻會被抑制。且處理誘導傷害防禦機制相關的分子,如:過氧化氫(hydrogen peroxide, H2O2)、一氧化氮(nitric oxide, NO)、甲基茉莉酸(Methyl jasmonate, MeJA)、離層酸(abscisic acid, ABA)及乙烯(ethylene),也會抑制miR828的表現。我們再藉由不同誘導劑與抑制劑探討miR828表現情形進而探討其訊息傳遞的過程,推測植物傷害後,促使NO與MeJA生合成,而MeJA也會而活化NOS產生NO,而NO再活化NADPH oxidase產生H2O2,或者MeJA本身也會透過過其他分子,而抑制miR828表現進而調控miR828標的基因表現。此外,ethylene也會透過MeJA抑制miR828表現,而一氧化碳(carbon monoxide, CO)會在之中清除H2O2進而抑制傳遞過程。而miRNA藉由序列互補性辨識標的基因,進而對標的基因進行靜默 (silencing)。我們利用cDNA clone釣取到三個可能的miR828標的基因,分別為receptor protein kinase (RPK)、TLD和MYB。藉由傷害後因miR828表現被抑制而減少對標的基因mRNA降解或降低抑制標的基因蛋白質轉譯,使得標的基因的表現進而參與傷害防禦調控機制。 | zh_TW |
dc.description.abstract | MicroRNAs (miRNAs) contain approximate 22 nucleotides that post-transcriptionally regulate gene expression by directing mRNA cleavage or translational inhibition. Currently, more than one hundred miRNAs that play multiple roles in growth, development, and stress responses of plants have been identified in Arabidopsis thaliana. Here, I investigated the expression patterns of miR159, miR407, and miRNA828 upon wounding in sweet potato. The expression of miR407 was undetectable in leaves and stems. However, miR159 was expressed in stems and, at low levels, in leaves, whereas miR828 was expressed in leaves and undetectable in stems. Furthermore, the expression of miR828 was decreased upon wounding in sweet potato. Additionally, several defensive responding compounds, including hydrogen peroxide (H2O2), nitric oxide (NO), methyl jasmonate (MeJA), abscisic acid (ABA), and ethylene also suppressed miR828 expression. However, carbon monoxide (CO) suppressed repression of miR828 by wounding. Interactions among these defensive responding compounds for the regulation of miR828 were also studied. Results suggested that wounding stimulates the biosynthesis of MeJA and NO. Then, MeJA activates NO synthase to generate NO. Finally, NO inspires the activity of NADPH oxidase to generate H2O2, which suppress miR828 expression. In addition to miR828, three potential miR828 targeted genes, receptor protein kinase (RPK), TLD, and MYB were isolated and were up-regulated by wounding. In addition, RPK, TLD, and MYB were regulated by NO and H2O2 when miR828 was repressed. These results imply that wounding represses the expression of miR828, and consequently regulates the expression of RPK, TLD, and MYB genes to protect plants from damage. | en |
dc.description.provenance | Made available in DSpace on 2021-05-20T20:37:52Z (GMT). No. of bitstreams: 1 ntu-97-R95b42004-1.pdf: 1787155 bytes, checksum: 0f29258cadf50c7aa7f329af3e112db5 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 中文摘要
英文摘要 第一章 前言 一、植物的防禦機制•••••••••••••••••••••••••••••••••••••• 1 二、miRNA的作用與機制•••••••••••••••••••••••••••••••••• 3 三、實驗方向與目的•••••••••••••••••••••••••••••••••••••• 7 第二章 材料與方法 一、材料••••••••••••••••••••••••••••••••••••••••••••• 8 二、實驗材料處理與藥劑••••••••••••••••••••••••••••••••••• 8 三、Total RNA萃取•••••••••••••••••••••••••••••••••••••• 9 四、Small RNA電泳•••••••••••••••••••••••••••••••••••••• 9 五、北方墨點法分析•••••••••••••••••••••••••••••••••••••• 9 六、Rapid Amplification of cDNA Ends (RACE)••••••••••••••••••••• 11 七、質體構築•••••••••••••••••••••••••••••••••••••••••• 13 八、Reverse transcription-polymerase chain reaction (RT-PCR)••• ••••••••• 14 九、Small RNA釣取•••••••••••••••••••••••••••••••••••••• 15 第三章 結果 一、miRNA在甘藷傷害表現之確認•••••••••••••••••••••••••••• 18 二、miR828訊息傳導••••••••••••••••••••••••••••••••••••• 19 三、miR828標的基因••••••••••••••••••••••••••••••••••••• 23 四、甘藷miRNA釣取••••••••••••••••••••••••••••••••••••• 26 第四章 討論 一、 miR159、miR407、miR828表現情形••••••••••••••••••••••••• 27 二、miR828的訊息傳遞過程•••••••••••••••••••••••••••••••• 28 三、miR828標的基因表現情形••••••••••••••••••••••••••••••• 32 四、結論••••••••••••••••••••••••••••••••••••••••••••• 35 圖表••••••••••••••••••••••••••••••••••••••••••••••••••••••• 36 參考文獻•••••••••••••••••••••••••••••••••••••••••••••••••••• 54 | |
dc.language.iso | zh-TW | |
dc.title | 探討microRNA在甘薯傷害的防禦機制 | zh_TW |
dc.title | Study of microRNAs involved in wounding response
in sweet potato | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 邱子珍,吳克強,鄭秋萍 | |
dc.subject.keyword | 甘薯,傷害,microRNA,標的基因,靜默, | zh_TW |
dc.subject.keyword | microRNA,miR828,sweet potato,target gene,wounding, | en |
dc.relation.page | 64 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2008-07-28 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-97-1.pdf | 1.75 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。