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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 靳宗洛 | zh_TW |
| dc.contributor.advisor | Tsung-Luo Jinn | en |
| dc.contributor.author | 王胤棨 | zh_TW |
| dc.contributor.author | Yin-Chi Wang | en |
| dc.date.accessioned | 2025-02-27T16:19:45Z | - |
| dc.date.available | 2025-02-28 | - |
| dc.date.copyright | 2025-02-27 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-02-10 | - |
| dc.identifier.citation | Alinsug, M.V., Yu, C.W., and Wu, K. (2009). Phylogenetic analysis, subcellular localization, and expression patterns of RPD3/HDA1 family histone deacetylases in plants. BMC Plant Biology 9, 37.
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HISTONE DEACETYLASE6 interacts with FLOWERING LOCUS D and regulates flowering in Arabidopsis. Plant Physiology 156, 173-84. Xu, Y., Li, Q., Yuan, L., Huang, Y., Hung, F.Y., Wu, K., and Yang, S. (2022). MSI1 and HDA6 function interdependently to control flowering time via chromatin modifications. Plant Journal 109, 831-843. Zang, D., Wang, J., Zhang, X., Liu, Z., and Wang, Y. (2019). Arabidopsis heat shock transcription factor HSFA7b positively mediates salt stress tolerance by binding to an E-box-like motif to regulate gene expression. Journal of Experimental Botany 70, 5355-5374. Zhou, J.J., Cho, J.S., Han, H., Blitz, I.L., Wang, W., and Cho, K.W.Y. (2023). Histone deacetylase 1 maintains lineage integrity through histone acetylome refinement during early embryogenesis. Elife 27, e79380. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/97129 | - |
| dc.description.abstract | 先前研究已經闡明部分與植物熱逆境反應相關的轉錄調控網絡，然而關於轉錄靜默和染色質重塑的機制仍然未知。組蛋白去乙醯基酶 (HDACs) 是與轉錄靜默緊密相連的關鍵染色質調控因子，對植物適應不良環境至關重要。阿拉伯芥微陣列分析顯示，熱逆境誘導HDA5的表達，但其功能與機制仍然不明。在本研究中透過兩種熱耐性測試：基礎耐熱性及後天耐熱性確認HDA5在熱逆境反應途徑中作為負調控因子。進一步透過蛋白交互作用實驗發現，在19個熱休克因子（HSFs）中，HDA5會與HSFA1d，HSFA8，HSFA9以及HSFB2b在細胞核中有相互作用。qPCR分析表明，在hda5-1突變株中，與野生型相比，多個HSFs的表達量增加，並且其下游基因HSP22與HSP101的表現量顯著增加。ChIP-qPCR分析發現，在hda5-1突變體中，HSP22啟動子的H3K9乙醯化的程度顯著提升，而在HSP101啟動子則未出現變化。此外，HSP22啟動子上的H3K9乙醯化與H3K4二甲基化的豐度之間存在拮抗關係，且HSP101啟動子的H3K4二甲基化的程度呈下降趨勢。在原生質體次細胞定位實驗中中發現HDA5在正常生長環境下出現在細胞核中，在熱逆境的環境下則是完全轉移至細胞核外，這表明HDA5可能間接調控熱相關基因 （heat-responsive genes）。Dual-luciferase reporter實驗顯示，HDA5與HSFB2b共同作用，顯著抑制了HSP22的轉錄活性。根據上述研究結果推測，HDA5在熱反應中可能透過非組蛋白為目標的蛋白質去乙醯化（deacetylation）與調控組蛋白修飾相關蛋白來實現對轉錄組的轉錄沉默，而非直接參與組蛋白修飾（histone modification）。 | zh_TW |
| dc.description.abstract | Research has highlighted the complex regulatory networks involved in plant heat shock responses (HSR), yet the mechanisms underlying chromatin remodeling and transcriptional silencing during HS adaptation remain elusive. Histone deacetylases (HDACs) are key chromatin regulators linked to transcriptional silencing, essential for plant resilience in adverse environments. Arabidopsis microarray data indicates that heat stress induces HDA5, but its precise role remains unclear. This study reveals that HDA5 functions as a negative regulator in the heat stress response and that thermotolerance tests confirm its regulatory role under both baseline and acquired heat stress conditions. Further analysis of protein-protein interactions data showed that among 19 heat shock factors (HSFs), HDA5 interacts with HSFA1d, HSFA8, HSFA9, and HSFB2b in the nucleus. qPCR analysis revealed that in the hda5-1 mutant, several HSFs were upregulated compared to the wild type (Col-0), with downstream genes HSP22 and HSP101 showing significant increases in expression. ChIP-qPCR analysis revealed an increase in H3K9 acetylation at the HSP22 loci in the hda5-1 mutant, while no changes were observed at the HSP101 loci. Additionally, an antagonistic relationship between H3K9 acetylation and H3K4 dimethylation was observed at the HSP22 loci, accompanied by a downtrend in H3K4 dimethylation at the HSP101 loci. Subcellular localization studies showed that HDA5 relocates from the nucleus to the cytoplasm under HS, suggesting that HDA5 may regulate heat-responsive genes indirectly. Dual-luciferase reporter assays revealed that HDA5, in conjunction with HSFB2b, significantly inhibits the transcriptional activity of HSP22. These findings propose a novel role for HDA5 in transcriptional silencing during HS, potentially through the deacetylation of non-histone proteins or modulation of histone modification-related proteins, rather than direct histone modification. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-02-27T16:19:45Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-02-27T16:19:45Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 致謝 i
摘要 ii Abstract iv List of figures viii List of tables x Abbreviations xi Introduction 1 Heat Shock Response (HSR) and Thermotolerance 1 Heat Shock Factors (HSFs) and Transcriptional Regulation 4 Heat Shock Proteins (HSPs) 8 Histone Acetylation and Methylation 10 Histone Deacetylases (HDACs) 12 Functions of HDA5 13 Motivation and Objectives 14 Material and Methods 16 Plant Materials and Growth Conditions 16 DNA/RNA Preparation, cDNA Synthesis and qRT-PCR 17 Thermotolerance Tests 18 Western Blot Analysis and Protein Abundancy Assay 18 Agro-Infiltration in Nicotiana benthamiana Leaves 19 Arabidopsis Mesophyll Protoplasts Isolation and Plasmid Transfection 20 Bimolecular Fluorescence Complementation (BiFC) Assay 21 Yeast Two Hybrid (Y2H) 22 Split-Luciferase Complementation Imaging (LCI) Assay 23 Nuclear-Cytoplasmic Fractionation 23 Chromatin Immunoprecipitation (ChIP)-qPCR 25 Dual Luciferase Reporter (DLR) Assay 27 Results 28 Gene Expression Patterns of Arabidopsis HDA5 Under Heat Stress Conditions 28 Characterization of the hda5 Knock-out Line and pCaMV 35S/ HDA5 Native Promoter-Driven HDA5 Transgenic Lines 28 hda5-1 Shown Insensitive Phenotypes Toward Multiple HS Conditions 29 Interactions Between HDA5 and Heat Shock Factors (HSFs). 31 Gene Expression Profiles of HS- Responsive Genes in hda5-1. 33 Accumulation of HSP101 and Acetyl-H3 Levels in hda5-1 During Heat Stress 34 HDA5 Participating in the Regulation of the HSP18.2, HSP22 and HSP101 Loci in HS 36 HDA5 Is Relocated from the Nucleus to the Cytosol under Heat Stress Conditions. 39 HDA5 and HSFB2b Repress the Transcriptional Activity of HSP22. 40 Discussion 41 HDA5 Functions as a Negative Regulator in HSR. 41 HDA5-Mediated Regulation of Heat Shock Factors (HSFs) and Heat Shock Proteins (HSPs) Across Multiple Pathways. 43 HDA5 Interacts with HSFB2b. 46 HDA5 Affects the Histone Modifications of HSP18.2, HSP22 and HSP101 loci. 47 HDA5 Completely Relocated Outside the Nucleus During HS. 48 HDA5 May Activates HSFB2b to Repress the HSP22 via Deacetylation. 49 HDA5 is Involved in Regulating Histone Modification-Related Proteins in HS. 50 Perspective and Future Works 51 Tables 53 Figures 58 Supplemental Figures 85 References 86 Appendix 92 | - |
| dc.language.iso | en | - |
| dc.subject | 表觀遺傳 | zh_TW |
| dc.subject | 熱休克蛋白 | zh_TW |
| dc.subject | 熱休克轉錄因子 | zh_TW |
| dc.subject | 耐熱性 | zh_TW |
| dc.subject | 非生物逆境 | zh_TW |
| dc.subject | 甲基化 | zh_TW |
| dc.subject | 去乙醯基化 | zh_TW |
| dc.subject | 去乙醯基蛋白 | zh_TW |
| dc.subject | 阿拉伯芥 | zh_TW |
| dc.subject | heat shock protein | en |
| dc.subject | Arabidopsis | en |
| dc.subject | epigenetic | en |
| dc.subject | histone deacetylase | en |
| dc.subject | deacetylation | en |
| dc.subject | methylation | en |
| dc.subject | abiotic stress | en |
| dc.subject | thermotolerance | en |
| dc.subject | heat shock factor | en |
| dc.title | 阿拉伯芥組蛋白去乙醯基酶HDA5在熱逆境中之功能性研究 | zh_TW |
| dc.title | Functional study of histone deacetylase HDA5 in response to thermal stress in Arabidopsis | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 鄭秋萍;鄭石通;楊健志;葉靖輝 | zh_TW |
| dc.contributor.oralexamcommittee | Chiu-Ping Cheng;Shih-Tong Jeng;Chien-Chih Yang;Ching-Hui Yeh | en |
| dc.subject.keyword | 阿拉伯芥,表觀遺傳,去乙醯基蛋白,去乙醯基化,甲基化,非生物逆境,耐熱性,熱休克轉錄因子,熱休克蛋白, | zh_TW |
| dc.subject.keyword | Arabidopsis,epigenetic,histone deacetylase,deacetylation,methylation,abiotic stress,thermotolerance,heat shock factor,heat shock protein, | en |
| dc.relation.page | 98 | - |
| dc.identifier.doi | 10.6342/NTU202500611 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2025-02-11 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 植物科學研究所 | - |
| dc.date.embargo-lift | 2025-02-28 | - |
| 顯示於系所單位： | 植物科學研究所 | |
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