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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王兆麟 | zh_TW |
| dc.contributor.advisor | Jaw-Lin Wang | en |
| dc.contributor.author | 薛尚怡 | zh_TW |
| dc.contributor.author | Shang-Yi Hsueh | en |
| dc.date.accessioned | 2024-09-15T16:52:40Z | - |
| dc.date.available | 2024-09-16 | - |
| dc.date.copyright | 2024-09-15 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-08-08 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/95701 | - |
| dc.description.abstract | 近年來，許多研究團隊開始將低強度超音波作為一種非侵入性的腦刺激工具，有許多研究指出低強度超音波具有神經調節特性，並且已經開始應用於治療腦部疾病上，而神經性聽力損失目前並無有效治療方法。既然低強度超音波能夠調節神經活性，或許具有成為治療神經性聽力損失方法的潛力，因此本研究試圖探討低強度超音波對於聽覺系統的影響，特別是中樞聽覺路徑。研究對象其一為中樞聽覺路徑起始的耳蝸核，而由於多項證據顯示超音波能夠影響海馬迴的神經活性，並且過往實驗室也針對海馬迴進行了許多研究，因此研究對象其二為海馬迴。超音波調節神經的機制被認為是透過機械力敏感通道調控，根據過往實驗室研究結果，我們認為N-甲基-D-天門冬胺酸受體(NMDAR)具有受超音波調控的潛力，因此本研究還會針對海馬迴中的NMDAR進行探討。
本研究使用c-Fos作為神經活性標記，並且利用c-Fos TRAP2小鼠來探討活體內神經活化的現象。研究結果顯示，低強度超音波會促進耳蝸核與海馬迴的c-Fos表現量上升，影響其神經活化，也會影響耳蝸核的音調排列結構，但不會使耳蝸核與海馬迴在兩次同樣刺激下產生的神經網路重疊率提升，推斷沒有長期影響；針對超音波與NMDAR的關係性，研究結果指出低強度超音波會促進海馬迴NMDAR磷酸化，並且超音波促進的神經活化會受NMDAR抑制而降低。綜上所述，本研究結果顯示低強度超音波可影響神經活性，且具備對NMDAR潛在的調節性。 | zh_TW |
| dc.description.abstract | In recent years, many research teams have explored using low-intensity ultrasound as a non-invasive brain stimulation tool. Numerous studies have investigated its applications in treating brain diseases. Sensorineural hearing loss (SNHL), which may result from central nervous system (CNS) damage, currently lack effective treatments. Given that low-intensity ultrasound can regulate neuronal activity, it may have the potential to treat SNHL. Therefore, this study aims to investigate the effects of low-intensity ultrasound on CNS, especially on the central auditory pathway. One focus of this study is the cochlear nucleus, the starting point of the central auditory pathway. Additionally, previous laboratory studies have extensively examined the ultrasound effects on the hippocampus, thus another focus will be the hippocampus. The mechanism by which ultrasound modulates neuronal activity is believed to involve regulating mechanosensitive channels. Based on previous laboratory findings, we hypothesize that N-Methyl-D-Aspartate Receptors (NMDAR) may be regulated by ultrasound. Thus, this study also investigates the response of NMDAR under ultrasound stimulation.
We use c-Fos as a neuronal activity marker, and utilize c-Fos TRAP2 mice to study neural activation in vivo. The results showed a significant immediate increase in c-Fos expression in both the cochlear nucleus and the hippocampus under ultrasound stimulation; however, no significant difference was observed in the overlap ratio of neural networks generated by the same stimulus. This indicates that ultrasound stimulation can immediately increase neuronal activity but with limitations in repeatedly activating the same group of neurons. Moreover, the 12kHz band pattern labeled by c-Fos in the cochlear nucleus was consistently wider under ultrasound stimulation. Regarding the relationship between ultrasound and NMDAR, the results showed that low-intensity ultrasound could promote NMDAR phosphorylation in the hippocampus, and the neuronal activation induced by ultrasound was reduced by NMDAR inhibition. In conclusion, these findings suggest that low-intensity ultrasound may affect neuronal activation and demonstrate the potential regulatory effect of ultrasound on NMDAR. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-09-15T16:52:40Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-09-15T16:52:40Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 I
致謝 II 摘要 III Abstract IV 參與計畫 V 目次 VI 圖次 VIII 表次 X 第一章 緒論 1 1.1 為甚麼要用超音波刺激腦 1 1.2 腦與神經細胞 2 1.3 腦區介紹 2 1.3.1 聽覺與耳蝸核 2 1.3.2 海馬迴 5 1.4 機械力敏感通道 6 1.5 超音波參數定義 8 1.6 研究目的 10 1.7 研究中檢測的蛋白質與通道 11 1.7.1 c-Fos 11 1.7.2 NMDAR 12 第二章 材料與方法 14 2.1 研究方法介紹 14 2.2 成鼠超音波腦刺激實驗 15 2.2.1 刺激系統 15 2.2.2 超音波探頭聲強度與升溫量測 18 2.2.3 c-Fos TRAP2機制 20 2.2.4 實驗設計 21 2.2.5 腦切片樣本製備 23 2.2.6 生物檢測法1：免疫螢光染色 (Immunofluorescence) 28 2.3 通道刺激/抑制實驗-組織 31 2.3.1 刺激系統 31 2.3.2 刺激裝置聲強度及升溫量測 32 2.3.3 蛋白質樣本製備 34 2.3.4 生物檢測法2：西方墨點法 (Western blot) 34 2.4 通道刺激/抑制實驗-動物 38 2.4.1 實驗設計 38 2.4.2 刺激系統與樣本製備 38 2.4.3 免疫螢光染色與分析方法 38 第三章 實驗結果 39 3.1 成鼠超音波腦刺激實驗-短期 39 3.1.1 耳蝸核短期影響 40 3.1.2 海馬迴短期影響 40 3.1.3耳蝸核音調排列結構 41 3.2 成鼠超音波腦刺激實驗-長期 45 3.2.1 耳蝸核長期影響 45 3.2.2 海馬迴長期影響 47 3.3 通道刺激/抑制實驗 48 3.3.1 NMDAR磷酸化對超音波的反應(組織) 48 3.3.2超音波刺激下神經活性受NMDAR抑制影響(動物) 49 第四章 討論 52 第五章 結論與未來展望 58 5.1結論 58 5.2未來展望 58 第六章 參考文獻 59 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 海馬迴 | zh_TW |
| dc.subject | NMDAR | zh_TW |
| dc.subject | 耳蝸核 | zh_TW |
| dc.subject | 神經調節 | zh_TW |
| dc.subject | 低強度超音波 | zh_TW |
| dc.subject | NMDAR | en |
| dc.subject | low-intensity ultrasound | en |
| dc.subject | neuromodulation | en |
| dc.subject | cochlear nucleus | en |
| dc.subject | hippocampus | en |
| dc.title | 耳蝸核與海馬迴的神經細胞對低強度超音波之反應 | zh_TW |
| dc.title | The Response of Neurons in Cochlear Nucleus and Hippocampus under the Low-Intensity Ultrasound Stimulation | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 吳玉威;楊世斌;吳振吉 | zh_TW |
| dc.contributor.oralexamcommittee | Yu-Wei Wu;Shi-Bing Yang;Chen-Chi Wu | en |
| dc.subject.keyword | 低強度超音波,神經調節,耳蝸核,海馬迴,NMDAR, | zh_TW |
| dc.subject.keyword | low-intensity ultrasound,neuromodulation,cochlear nucleus,hippocampus,NMDAR, | en |
| dc.relation.page | 64 | - |
| dc.identifier.doi | 10.6342/NTU202403808 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2024-08-12 | - |
| dc.contributor.author-college | 工學院 | - |
| dc.contributor.author-dept | 醫學工程學系 | - |
| 顯示於系所單位： | 醫學工程學研究所 | |
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| ntu-112-2.pdf | 1.95 MB | Adobe PDF | 檢視/開啟 |
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