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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 童心欣 | zh_TW |
| dc.contributor.advisor | Hsin-Hsin Tung | en |
| dc.contributor.author | 顏逢毅 | zh_TW |
| dc.contributor.author | Feng-Yi YEN | en |
| dc.date.accessioned | 2024-09-05T16:13:51Z | - |
| dc.date.available | 2024-09-06 | - |
| dc.date.copyright | 2024-09-05 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-08-12 | - |
| dc.identifier.citation | Berney, M., Vital, M., Hülshoff, I., Weilenmann, H.-U., Egli, T., & Hammes, F. (2008). Rapid, cultivation-independent assessment of microbial viability in drinking water. Water research, 42(14), 4010-4018.
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Using discrete and online ATP measurements to evaluate regrowth potential following ozonation and (non) biological drinking water treatment. Water research, 154, 377-386. Deininger, R., & Lee, J. (2005). Rapid detection of bacteria in drinking water. Modern Tools and Methods of Water Treatment for Improving Living Standards: Proceedings of the NATO Advanced Research Workshop on Modern Tools and Methods of Water Treatment for Improving Living Standards Dnepropetrovsk, Ukraine 19–22 November 2003, Delahaye, E., Welte, B., Levi, Y., Leblon, G., & Montiel, A. (2003). An ATP-based method for monitoring the microbiological drinking water quality in a distribution network. Water Research, 37(15), 3689-3696. Directive, C. (1998). On the quality of water intended for human consumption. Official Journal of the European Communities, 330, 32-54. El-Chakhtoura, J., Prest, E., Saikaly, P., van Loosdrecht, M., Hammes, F., & Vrouwenvelder, H. (2015). 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/95335 | - |
| dc.description.abstract | 供水系統中的生物穩定性是重要的生物安全指標，培養基培養法檢測自來水中的總菌數無法精確反應加氯的自來水系統生物穩定性。隨著技術進步，三磷酸腺苷(adenosine triphosphate , ATP)檢測逐漸成熟，相較於傳統塗盤法更靈敏方便且能在短時間內獲得結果。本研究旨在開發一種液態培養方法，並利用三磷酸腺苷(ATP)進行微生物活性檢測取代塗盤法，以提高傳統自來水總菌數檢測方法的檢出率。首先，針對台灣自來水，使用國內外常用的總異營菌數培養基塗盤法進行比較，以瞭解不同培養基檢出量的差異。結果顯示R2A培養基所獲得的菌落數較高；後續研究則採用R2A培養液加入自來水樣品中進行液態培養之實驗。利用液態培養方法結合ATP檢測，評估液態培養替代塗盤法之可行性。利用液態培養進行最大生長速率及最大生長量之實驗檢測，所獲之ATP濃度與初始菌落數進行線性回歸，結果分別是(R^2=0.37)、(R^2=0.71)，顯示利用最大生長量的培養方法可以有效的提高ATP濃度與R2A菌落數間的關係。但利用T-TEST、Wilcoxon檢定結果顯示最大生長量所獲得之公式並無法有效的利用ATP濃度來推估初始菌落數，但在加氯後的自來水中經過液態培養方法後，可以有效的提高ATP濃度與菌落數間的關係。因此，若要以ATP做為消毒後的自來水之生物活性檢測，仍需要有更多的研究來進行補強。 | zh_TW |
| dc.description.abstract | Biostability in water supply systems is an important microbiological safety indicator. Traditional culture-based methods that measure total bacterial counts in tap water cannot accurately reflect the biostability of chlorinated water systems. With technological advancements, adenosine triphosphate (ATP) testing offering a more sensitive and convenient alternative to the traditional plate count method and provides results in a shorter time. This study aims to develop a different type of liquid culture method and use ATP to measure microbial activity, replacing the plate count method to improve the detection rate of traditional total microbial count methods in tap water. Initially, the study compared the plate count methods for heterotrophic bacteria with Taiwan's tap water to determine the differences between R2A and PCA media. The results indicated that the R2A medium yielded higher colony counts; subsequent experiments involved adding tap water samples to R2A broth for liquid culturing. This liquid culturing method, combined with ATP testing, was evaluated for its feasibility to replace the plate culture method. Experiments measuring maximum growth rates and biomass using liquid culture showed that ATP concentrations correlated with initial colony counts with linear regression results of R^2=0.37 and R^2=0.71, respectively. The results demonstrated that using maximum biomass culture methods could effectively enhance the relationship between ATP concentration and R2A colony counts. However, T-TEST and Wilcoxon test indicated that the formulas obtained from maximum biomass could not effectively use ATP concentration to estimate initial colony counts. However, in chlorinated tap water, the liquid culture method still effectively improved the relationship between ATP concentration and colony counts. Therefore, further research is needed to strengthen the use of ATP as a measure of biological activity in disinfected tap water. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-09-05T16:13:51Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-09-05T16:13:51Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 誌謝 i
摘要 ii Abstract iii 目次 v 圖次 vii 表次 viii 第一章 前言 1 1-1研究背景 1 1-2研究假說與目的 3 第二章 文獻回顧 4 2-1 自來水供水系統微生物與水質 4 2-1.1 自來水供水系統生物穩定性 4 2-1.2導致供水系統中水質變化的因素 5 2-1.3 自來水微生物造成的健康及衛生風險 5 2-2 飲用水微生物監測 7 2-2.1國際與國內的自來水微生物監測指標差異 7 2-2.2自來水微生物傳統檢測方法與新興方法的差異 11 第三章 材料與方法 15 3-1 實驗架構 15 3-2 水樣採集位置 16 3-3 水樣採集運送及處理 20 3-3.1 水樣採集、運輸及保存 20 3-4 水質及微生物分析方法 20 3-4.1 pH、溫度、濁度、總氯及自由餘氯檢測方法 20 3-4.2 水中總異營菌數 (Heterotrophic Plate Count, HPC)、水中總菌落數 (Total Count) 21 3-4.3 16s rRNA基因檢測方法 22 3-4.4生物可利用有機碳(Assimilable Organic Carbon , AOC)的檢測 22 3-4.5自來水微生物三磷酸腺苷檢測 23 3-4.6 新型液態培養方法 24 3-5數據處理及統計分析 25 第四章 結果 26 4-1供水系統生物穩定性結果 26 4-1.1 供水系統水質結果之差異 26 4-1.2 我國現行檢測方法的總菌落數與APHA9215C方法的總異營數結果差異 26 4-1.3 供水系統菌落數與AOC濃度之關係 30 4-2 ATP分析方法與菌落數之實驗結果 31 4-2.1 ATP檢測方法與培養法的檢出率差異 31 4-2.2 自由餘氯濃度與生物穩定性檢測結果的相關性分析 33 4-2.3供水系統中ATP濃度與培養結果之關係 35 4-3 液態培養方法生長曲線實驗結果 37 4-3.1最佳生長速率之生長曲線結果 37 4-3.2最大生長量之生長曲線結果 39 4-4 最佳生長速率與最大生長量之培養結果 41 4-4.1最佳生長速率之HPC/Total Count菌落數與ATP濃度之相關性 41 4-4.2 液態培養數據結果 43 4-4.3液態培養實驗最大生長量實驗結果 45 4-5 最大生長量ATP與初始菌落之回歸結果 47 4-5 ATP濃度與R2A初始菌落數之回歸 47 第五章:討論 49 5-1自來水水體中微生物檢測方法 49 5-2結合ATP分析方法的液態培養 51 6-1結論 53 6-2建議 54 參考文獻 55 附錄 71 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 總菌落數 | zh_TW |
| dc.subject | 生物穩定性 | zh_TW |
| dc.subject | 餘氯 | zh_TW |
| dc.subject | 三磷酸腺苷 | zh_TW |
| dc.subject | 總異營數 | zh_TW |
| dc.subject | 自來水供水系統 | zh_TW |
| dc.subject | free chlorine | en |
| dc.subject | heterotrophic plate count | en |
| dc.subject | adenosine triphosphate | en |
| dc.subject | total count | en |
| dc.subject | biostability | en |
| dc.subject | Drinking water distribution system | en |
| dc.title | 液態培養結合ATP檢測用於評估自來水生物穩定性 | zh_TW |
| dc.title | Evaluation of biostability in tap water by liquid culture and ATP measurement | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 謝季吟;陳冠中;吳佳真 | zh_TW |
| dc.contributor.oralexamcommittee | Chi-Ying Hsieh;Kuan-chung Chen;Chia-Chen Wu | en |
| dc.subject.keyword | 自來水供水系統,總菌落數,總異營數,三磷酸腺苷,餘氯,生物穩定性, | zh_TW |
| dc.subject.keyword | Drinking water distribution system,total count,heterotrophic plate count,adenosine triphosphate,free chlorine,biostability, | en |
| dc.relation.page | 93 | - |
| dc.identifier.doi | 10.6342/NTU202403603 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2024-08-13 | - |
| dc.contributor.author-college | 工學院 | - |
| dc.contributor.author-dept | 環境工程學研究所 | - |
| 顯示於系所單位： | 環境工程學研究所 | |
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