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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 陳培哲 | zh_TW |
| dc.contributor.advisor | Pei-Jer Chen | en |
| dc.contributor.author | 梁維綸 | zh_TW |
| dc.contributor.author | Wei-Lun Liang | en |
| dc.date.accessioned | 2024-08-28T16:15:38Z | - |
| dc.date.available | 2024-08-29 | - |
| dc.date.copyright | 2024-08-28 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-08-02 | - |
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Analytical chemistry, 2011. 83(22): p. 8604-8610. 88. Americo, J.L., P.L. Earl, and B. Moss, Droplet digital PCR for rapid enumeration of viral genomes and particles from cells and animals infected with orthopoxviruses. Virology, 2017. 511: p. 19-22. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/95099 | - |
| dc.description.abstract | B型肝炎病毒（HBV）是全球範圍內導致慢性肝病和肝細胞癌的主要原因。理解HBV感染的早期階段，特別是HBV RNA的合成和出口，對於開發有效的診斷和治療策略至關重要。病毒通常擁有獨特的RNA出口機制，使它們能夠成功地利用宿主細胞的機制進行RNA的轉運和表達。例如，HIV通過Rev-RRE系統促進未剪接RNA的出口，Mason-Pfizer猴病毒（MPMV）則利用CTE元素進行RNA的核輸出。本研究旨在探討HBV RNA在感染初期的合成動力學，並希望發現HBV RNA出口的可能機制。利用Northern blot偵測HBV mRNAs能夠在感染後48小時看見3.5 kb preC/pgRNA, 2.4 kb PreS1 RNA, and 2.1 kb PreS2/S RNA 的存在，更進一步以RT-PCR發現在感染12和24小時後只出現剪接型RNA的訊號，並沒有未剪接RNA的存在，但還需要加以證明。另外，我們希望開發一種利用特殊的引子設計的RT-qPCR方法試圖在不處理DNaseI的狀況下偵測HBV RNAs，然而並無法找出合適的引子條件能夠完全避免HBV DNA干擾，所以我們進一步對RNA進行mRNA純化。最後以20 nM 3’Poly(dT)-Adaptor的引子濃度和純化mRNA的方式進行RT-qPCR能夠避免HBV DNA的干擾，然而此方法在偵測不同感染時間點的HBV mRNA靈敏度過低，而且測得的不同感染時間點的Ct值趨勢並不符合Northerm blot的結果。 | zh_TW |
| dc.description.abstract | Hepatitis B virus (HBV) is a major global cause of chronic liver disease and hepatocellular carcinoma. Understanding the early stages of HBV infection, particularly the synthesis and export of HBV RNA, is crucial for developing effective diagnostic and therapeutic strategies. Viruses often possess unique RNA export mechanisms that allow them to successfully utilize host cell machinery for RNA transport and expression. For example, HIV promotes the export of unspliced RNA through the Rev-RRE system, while Mason-Pfizer monkey virus (MPMV) uses the CTE element for RNA nuclear export. This study aims to investigate the kinetics of HBV RNA synthesis during the early stages of infection and to identify potential mechanisms for HBV RNA export. Northern blot analysis revealed the presence of 3.5 kb preC/pgRNA, 2.4 kb PreS1 RNA, and 2.1 kb PreS2/S RNA at 48 hours post-infection. Further RT-PCR analysis showed that only spliced RNA signals were detected at 12 and 24 hours post-infection, with no evidence of unspliced RNA, though further confirmation is needed. Additionally, we aimed to develop an RT-qPCR method using specially designed primers to detect HBV RNAs without DNase I treatment. However, suitable primer conditions to completely avoid HBV DNA interference could not be found. Therefore, we proceeded with mRNA purification. Finally, using a 20 nM 3’Poly(dT)-Adaptor primer concentration and purified mRNA for RT-qPCR successfully avoided HBV DNA interference. Nonetheless, this method had insufficient sensitivity for detecting HBV mRNA at different infection time points, and the Ct value trends did not align with Northern blot results. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-08-28T16:15:38Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-08-28T16:15:38Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 謝辭 i
摘要 ii ABSTRACT iii CONTENTS v CHAPTER 1: INTRODUCTION 1 1.1 Introduction of HBV 1 1.1.1 Epidemiology of HBV 1 1.1.2 HBV genome and structure 2 1.1.3 HBV life cycle 3 1.2 Cellular RNA processing 5 1.2.1 5’ capping 5 1.2.2 Splicing 5 1.2.3 3’ polyadenylation 6 1.3 RNAs export 6 1.3.1 Cellular RNA export 6 1.3.1.1 NXF1-mediated mRNA export 6 1.3.1.2 CRM1-mediated mRNA export 7 1.3.2 Viral RNA export 8 1.3.2.1 Human Immunodeficiency Virus-1 (HIV-1) 9 1.3.2.2 Mason–Pfizer Monkey Virus (MPMV) 11 1.3.2.3 Foamy Virus (FV) 11 1.3.2.4 Hepatitis B virus (HBV) 12 1.4 Hypothesis 13 CHAPTER 2: MATERIAL AND METHODS 15 2.1 Cell culture 15 2.2 Plasmids 15 2.3 Plasmid isolation 15 2.4 Antibodies 17 2.5 HBV genotype D wild-type preparation 17 2.6 Purification Dane particles via Heparin-Affinity Chromatography 18 2.7 Sucrose cushion 18 2.8 In vitro infection 19 2.9 Fractionation of cytoplasmic and nuclear fraction 19 2.10 Quantification of HBV DNA 20 2.11 RNA extraction 21 2.12 mRNA purification 22 2.13 RT-qPCR to quantification of HBV RNAs 22 2.14 RT-PCR to detect HBV spliced RNAs 23 2.15 BCA assay 23 2.16 Western blotting 24 2.17 Northern blot 25 CHAPTER 3: RESULTS 28 3.1 Optimal 3’ Poly(dT)-Adaptor Concentration for Specific Detection of HBV RNA via RT-qPCR: Balancing Sensitivity and Specificity at 20 nM 28 3.2 Enhanced Specificity in HBV RNAs detection via qPCR through mRNA purification 30 3.3 Validation of infected HepG2-NTCP-C4 cells mRNA purification and test RT-qPCR detection of HBV mRNAs in Infected HepG2-NTCP-C4 cells at different time points infection 31 3.4 The temporal kinetics of HBV mRNAs and core protein synthesis 34 3.5 RT-PCR detect HBV spliced RNAs at different time-point infection 37 3.6 The temporal distribution of HBV core Protein during time-point infection 38 CHAPTER 4: DISCUSSIONS 40 CHAPTER 5: FIGURES 48 5.1 Optimization of qPCR 3’Poly(dT)-Adaptor concentration using HepG2-AD38 cDNA and HBV construct for HBV RNA Detection 48 5.2 Validation of HepG2-AD38 cells mRNA purification and verification of no signal in No RT control for qPCR detection of purified HepG2-AD38 mRNA 50 5.3 Validation of infected HepG2-NTCP-C4 cells mRNA purification and RT-qPCR detection of HBV mRNAs in Infected HepG2-NTCP-C4 cells at different time points infection 53 5.4 The temporal kinetics of HBV mRNAs and core protein synthesis 55 5.5 Detection of spliced RNA types appearing at different infection time points via RT-PCR 57 5.6 The temporal distribution of HBV core Protein during time-point infection 59 CHAPTER 6: TABLES 61 6.1 Primer 61 CHAPTER 7: APPENDIX 62 7.1 Life cycle of HBV 62 7.2 Overview of HBV spliced RNA 63 7.3 Plasmid map- pCMV HBV genotype D Precore null 64 REFERENCES 65 | - |
| dc.language.iso | en | - |
| dc.subject | B型肝炎病毒 | zh_TW |
| dc.subject | 剪接型RNA | zh_TW |
| dc.subject | 定量反轉錄PCR | zh_TW |
| dc.subject | RNA輸出 | zh_TW |
| dc.subject | HBV 信使RNA | zh_TW |
| dc.subject | HBV mRNA | en |
| dc.subject | Real-time quantitative PCR | en |
| dc.subject | Hepatitis B virus | en |
| dc.subject | Spliced RNA | en |
| dc.subject | RNA export | en |
| dc.title | 在感染中HBV RNA合成的時間動力學 | zh_TW |
| dc.title | The temporal kinetics of HBV RNAs synthesis in infection | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 葉秀慧;陶秘華 | zh_TW |
| dc.contributor.oralexamcommittee | Shiou-Hwei Yeh ;Mi-Hua Tao | en |
| dc.subject.keyword | B型肝炎病毒,HBV 信使RNA,RNA輸出,定量反轉錄PCR,剪接型RNA, | zh_TW |
| dc.subject.keyword | Hepatitis B virus,HBV mRNA,RNA export,Real-time quantitative PCR,Spliced RNA, | en |
| dc.relation.page | 71 | - |
| dc.identifier.doi | 10.6342/NTU202402958 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2024-08-02 | - |
| dc.contributor.author-college | 醫學院 | - |
| dc.contributor.author-dept | 微生物學研究所 | - |
| dc.date.embargo-lift | 2026-08-01 | - |
| Appears in Collections: | 微生物學科所 | |
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| ntu-112-2.pdf Until 2026-08-01 | 1.84 MB | Adobe PDF |
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