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標題: | 水稻葉片Aryl Acylamidase活性與反射光譜關聯性之研究 Studies on the Relationship of Aryl Acylamidase Activity and Reflectance Spectra in the Leaves of Rice |
作者: | 黃渝雅 Yu-Ya Huang |
指導教授: | 黃文達 Wen-Dar Huang |
關鍵字: | Aryl acylamidase,除草靈,水稻,氮素,反射光譜,遙感探測, Aryl acylamidase,Propanil,rice,nitrogen,reflectance spectra,remote sensing, |
出版年 : | 2023 |
學位: | 碩士 |
摘要: | 除草靈 (Propanil) 為水稻萌後選擇性除草劑,水稻藉由所含有水解酵素Aryl acylamidase可以將Propanil分解,避免受Propanil的藥害。Aryl acylamidase除了能有效降解Propanil,在氮素代謝中也扮演重要角色,水稻的Aryl acylamidase活性會因品種與肥培管理而有所差異。因此本研究擬探討台灣秈、稉稻品種及不同氮素等級對水稻葉片Aryl acylamidase活性之影響,同時透過分析水稻葉片反射光譜,建立Aryl acylamidase酵素指數 (Enzyme index) 及預測模型,以期在未來能透過遙測等非破壞性方式,快速且大範圍的監測水稻Aryl Acylamidase活性。秈稉稻比較實驗之參試品種為台中秈10號、台中秈17號、桃園3號、桃園5號、桃園6號、台稉9號、台稉11號和台稉14號;氮素等級實驗參試品種為桃園3號、桃園5號、桃園6號及台稉11號,氮素處理等級分為80、120、160及200 kg ha-1共4級,並測定555nm下之吸光度來代表Aryl acylamidase活性大小。結果顯示,品種間Aryl acylamidase活性有顯著差異,稉稻品種高於秈稻。在氮素處理試驗中,可發現整體而言,氮素施用會提高各品種Aryl acylamidase活性,影響程度依品種而異。反射光譜結果中,4品種於520、680及750 nm附近為敏感波段,因此以上述3波長建立酵素指數。Aryl acylamidase活性與酵素指數迴歸分析結果顯示,各品種之預測模型決定係數皆高於0.9。本研究確立了Aryl acylamidase的活性測定方式,並透過品種比較及氮素處理試驗,確立以及在水稻品種間的差異性及和氮素的關聯。在反射光譜上,本研究成功建立Aryl acylamidase酵素指數及非破壞性Aryl acylamidase活性預測模型,而各模型皆有優秀的預測能力,顯示運用反射光譜監測Aryl acylamidase活性為可行之方法,提供水稻栽培系統中雜草管理之參考。 Propanil is a postemergence selective herbicide in rice fields. The hydrolyzing enzyme Aryl acylamidase in rice can degrade Propanil to avoid damage caused by herbicides. Aryl acylamidase activity in rice varies depending on the variety and fertilizer management. Therefore, this study aims to investigate the effects of indica and japonica rice varieties, as well as different nitrogen levels, on the activity of Aryl acylamidase in rice leaves in Taiwan. The study also seeks to establish Aryl acylamidase enzyme indices and predictive models by analyzing the reflectance spectrum of rice leaves. Thus, in the future, it is possible to monitor the Aryl acylamidase activity of rice in a non-destructive way and on a rapid and large scale, such as remote sensing. The rice varieties tested in the indica and japonica rice comparison experiment were Taichung Sen No. 10, Taichung Sen No. 17, Taoyuan No.3, Taoyuan No.6, Taikeng No.9, Taikeng No.11, and Taikeng No.14. The nitrogen level experiment was conducted on Taoyuan No.3, Taoyuan No.5, Taoyuan No.6, and Taikeng No.11. The nitrogen treatment levels had four levels: 80, 120, 160, and 200 kg ha-1, and the absorbance at 555 nm was measured to represent the Aryl acylamidase activity. The results showed significant differences in Aryl acylamidase activity among the varieties, with the japonica rice being higher than indica rice. The nitrogen treatment experiment found that overall, nitrogen application increased the activity of Aryl acylamidase in each variety, with the degree of effect varying by it. The results of the reflectance spectrum showed that the four varieties were sensitive around 520, 680, and 750 nm, so the enzyme indices were established from these wavelengths. The regression analysis of Aryl acylamidase activity and enzyme indices showed that the coefficient of determination in all varieties' prediction models was higher than 0.9. In this study, we determined the activity of Aryl acylamidase. The variability among rice varieties and the association with nitrogen were established through variety comparison and nitrogen treatment experiments. In the reflectance spectrum, Aryl acylamidase enzyme index and non-destructive Aryl acylamidase activity prediction models were successfully developed. Each model had an excellent predictive ability, indicating that using the reflectance spectrum to monitor Aryl acylamidase activity is feasible for weed management in rice cultivation systems. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/92554 |
DOI: | 10.6342/NTU202400750 |
全文授權: | 同意授權(限校園內公開) |
顯示於系所單位: | 農藝學系 |
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