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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林致廷 | zh_TW |
| dc.contributor.advisor | Chih-Ting Lin | en |
| dc.contributor.author | 吳晢勤 | zh_TW |
| dc.contributor.author | Che-Chin Wu | en |
| dc.date.accessioned | 2024-03-08T16:20:05Z | - |
| dc.date.available | 2024-03-09 | - |
| dc.date.copyright | 2024-03-08 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-02-19 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/92213 | - |
| dc.description.abstract | 本研究透過數學模型方法與實驗室晶片技術，分析微米級粒子在放射狀多相位電極作用下的運動現象。必揭示了粒子繞行電極旋轉之行為為一進動現象，且詳細分析了粒子在電場中的自轉與公轉，以及這些運動現象如何受到電場強度及粒子大小關係的影響。粒子以平行電極方向為自轉軸自轉，通過重力與傳統介電泳力提供角加速度，繞行電極中心公轉。當粒子在環形電極外圍時，電極間距寬於粒子大小，粒子主要受正旋度場加速；而在靠近中心電極時粒子大小大於電極間距，集中於電極表面的負旋度場主宰旋轉方向，開始出現與外圍相反的旋轉方向。
此外，研究進一步發現旅波介電泳力的本質是一種純旋度場，該力場可以持續傳輸能量提供粒子動能。在 3kHz 的螢光珠實驗中，我們觀測到粒子的動能持續增加至其旋轉速度超過每秒 30 幀的感光元件的捕捉頻率，我們應該以旋度場的角度去理解旅波介電泳現象。這種能量傳輸現象的發現，對於粒子動能的轉換提供了新的理解角度。 應用這一發現，我們成功地在實驗室晶片上展示了旋轉分離少量血球檢體的可行性，血球的分離效果在3kHz 時可以取得最好的應用。 | zh_TW |
| dc.description.abstract | This research delves into the dynamics of particles subjected to radial multi-phase electrode array, employing numerical simulations to elucidate the precessional movement of particles around electrodes. A detailed investigation into the particles' rotational and orbital behaviors under electric fields reveals the influence of electric field strength and particle size on these motions. Specifically, the study highlights the distinct rotational directions exhibited by particles when located at the electrode periphery versus near the electrode center, attributed to the differential field acceleration and reverse field dominance, respectively.
A significant finding is the identification of the traveling wave dielectrophoretic force as a solenoidal field, allowing continuous energy transmission to augment the kinetic energy of particles. From the experiment with 3kHz fluorescent beads, we observed that the particles' motion speed continuously increased beyond the capture capability of the imaging sensors at 30 frames per second. The discovery provides a new perspective on the conversion of particle kinetic energy, and also reveals that traveling wave dielectrophoresis still has strong application potential compared to traditional dielectrophoresis. This insight paved the way for demonstrating blood cell rotational separation on a lab chip, with optimal separation effects achieved at 3kHz, showcasing potential applications in small sample diagnostics. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-03-08T16:20:05Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-03-08T16:20:05Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | Verification Letter from the Oral Examination Committee i
Acknowledgements iii 摘要 v Abstract vii Contents ix List of Figures xiii Denotation xvii Chapter 1 Introduction 1 1.1 Preamble . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.2 Research Background . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.3 Research Motivation . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1.4 Research Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 1.5 Thesis Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Chapter 2 Literature Review 7 2.1 Lab-on-a-Chip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2.2 Electrodynamics . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.3 Dielectrophoresis . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2.3.1 Conventional Dielectrophoresis . . . . . . . . . . . . . . . . . . . . 11 2.3.2 Traveling-Wave Dielectrophoresis . . . . . . . . . . . . . . . . . . 15 2.3.3 Clausius-Mossotti Factor . . . . . . . . . . . . . . . . . . . . . . . 19 2.4 Electrical Double Layer . . . . . . . . . . . . . . . . . . . . . . . . 21 2.5 Electroosmosis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Chapter 3 Experimental System Setup and Methodology 27 3.1 Theory Derivation . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 3.1.1 Helmholtz Decomposition . . . . . . . . . . . . . . . . . . . . . . 29 3.2 Mathematical Simulation . . . . . . . . . . . . . . . . . . . . . . . . 30 3.3 Design and Fabrication of Circular Electrode Array . . . . . . . . . . 35 3.3.1 PCB Circuit Design . . . . . . . . . . . . . . . . . . . . . . . . . . 35 3.3.2 Fabrication of Glass Electrode Chips . . . . . . . . . . . . . . . . . 36 3.3.2.1 Electrode Design and Mask Production . . . . . . . . . 36 3.3.2.2 Surface Preparation of Glass Substrate . . . . . . . . . 37 3.3.2.3 Application of S1813 Photoresist . . . . . . . . . . . . 38 3.3.2.4 Exposure Using Karl Suss Aligner . . . . . . . . . . . 39 3.4 Microchannel Design and Fabrication Process . . . . . . . . . . . . . 39 3.4.1 Microfluidic Channel Design . . . . . . . . . . . . . . . . . . . . . 40 3.4.2 Silicon Wafer Master Mold Fabrication . . . . . . . . . . . . . . . . 40 3.4.3 PDMS Replication . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3.5 Packaging Technology . . . . . . . . . . . . . . . . . . . . . . . . . 46 3.5.1 Bonding Printed Circuit Board and Glass Electrode Chips . . . . . . 46 3.5.2 Bonding PDMS with Glass Chips . . . . . . . . . . . . . . . . . . 47 3.5.3 Encapsulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 3.6 Experimental Equipment . . . . . . . . . . . . . . . . . . . . . . . . 50 3.6.1 Signal Generator . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 3.6.2 Upright Microscope . . . . . . . . . . . . . . . . . . . . . . . . . . 50 3.7 Experimental Procedure . . . . . . . . . . . . . . . . . . . . . . . . 51 3.7.1 Solution Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . 51 3.7.1.1 D-Mannitol Preparation . . . . . . . . . . . . . . . . . 51 3.7.1.2 Blood Preparation . . . . . . . . . . . . . . . . . . . . 51 3.7.1.3 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . 52 3.7.2 Operating Procedures . . . . . . . . . . . . . . . . . . . . . . . . . 52 Chapter 4 Results and Discussion 53 4.1 Fluorescent Polystyrene Beads . . . . . . . . . . . . . . . . . . . . . 53 4.1.1 Observation under 3kHz, 3V . . . . . . . . . . . . . . . . . . . . . 53 4.1.2 Observation under 10kHz, 3V . . . . . . . . . . . . . . . . . . . . 54 4.1.3 Observation under 70kHz, 3V . . . . . . . . . . . . . . . . . . . . 54 4.1.4 Observation under 80kHz, 3V . . . . . . . . . . . . . . . . . . . . 55 4.1.5 Observation under 90kHz, 3V . . . . . . . . . . . . . . . . . . . . 55 4.1.6 Observation under 100kHz, 3V . . . . . . . . . . . . . . . . . . . . 56 4.2 Red Blood Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 4.2.1 Observation under 1kHz, 3V . . . . . . . . . . . . . . . . . . . . . 56 4.2.2 Observation under 2kHz, 3V . . . . . . . . . . . . . . . . . . . . . 57 4.2.3 Observation under 3kHz, 3V . . . . . . . . . . . . . . . . . . . . . 57 4.2.4 Observation under 5kHz, 3V . . . . . . . . . . . . . . . . . . . . . 58 4.2.5 Observation under 10kHz, 3V . . . . . . . . . . . . . . . . . . . . 58 4.2.6 Observation under 50kHz, 3V. . . . . . . . . . . . . . . . . . . . 59 4.2.7 Summery of the Observation . . . . . . . . . . . . . . . . . . . . . 59 Chapter 5 Conclusion and Future Work 61 5.1 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 5.2 Future Work . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 References 67 Appendix A — Formula 73 A.1 Expression of Mathematical Simulation . . . . . . . . . . . . . . . . 73 Appendix B — Source Code 75 B.1 Clausius-Mossoti Factor . . . . . . . . . . . . . . . . . . . . . . . . 75 B.2 Closed-Form of U and Z . . . . . . . . . . . . . . . . . . . . . . . . 76 B.3 Curl of TWDEP Plot . . . . . . . . . . . . . . . . . . . . . . . . . . 78 B.4 Unified DEP Simulation Plot . . . . . . . . . . . . . . . . . . . . . . 82 | - |
| dc.language.iso | en | - |
| dc.subject | 電動力學 | zh_TW |
| dc.subject | 介電泳 | zh_TW |
| dc.subject | 旅波介電泳 | zh_TW |
| dc.subject | 電旋轉 | zh_TW |
| dc.subject | 微流道 | zh_TW |
| dc.subject | 血球分離 | zh_TW |
| dc.subject | 實驗室晶片 | zh_TW |
| dc.subject | Electrorotation | en |
| dc.subject | Electrodynamics | en |
| dc.subject | Dielectrophoresis | en |
| dc.subject | Lab-on-a-chip Technology | en |
| dc.subject | Blood Cell Separation | en |
| dc.subject | Microfluidic Channel | en |
| dc.subject | Traveling Wave Dielectrophoresis | en |
| dc.title | 探討旅波介電泳對於介電溶液中粒子運動之影響 | zh_TW |
| dc.title | Exploring the Influence of Traveling-wave Dielectrophoresis on Particle Dynamics in Dielectric Solutions | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.coadvisor | 范育睿 | zh_TW |
| dc.contributor.coadvisor | Yu-Jui Fan | en |
| dc.contributor.oralexamcommittee | 李雨;張子璿;林詳淇 | zh_TW |
| dc.contributor.oralexamcommittee | U Lei;Tzu-Hsuan Chang;Shiang-Chi Lin | en |
| dc.subject.keyword | 電動力學,介電泳,旅波介電泳,電旋轉,微流道,血球分離,實驗室晶片, | zh_TW |
| dc.subject.keyword | Electrodynamics,Dielectrophoresis,Traveling Wave Dielectrophoresis,Electrorotation,Microfluidic Channel,Blood Cell Separation,Lab-on-a-chip Technology, | en |
| dc.relation.page | 86 | - |
| dc.identifier.doi | 10.6342/NTU202400726 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2024-02-19 | - |
| dc.contributor.author-college | 電機資訊學院 | - |
| dc.contributor.author-dept | 電子工程學研究所 | - |
| 顯示於系所單位： | 電子工程學研究所 | |
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