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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 閔明源 | zh_TW |
dc.contributor.advisor | Ming-Yuan Ming | en |
dc.contributor.author | 鄔雨銘 | zh_TW |
dc.contributor.author | Yu-Ming Wu | en |
dc.date.accessioned | 2024-03-08T16:14:43Z | - |
dc.date.available | 2024-03-09 | - |
dc.date.copyright | 2024-03-08 | - |
dc.date.issued | 2024 | - |
dc.date.submitted | 2024-02-17 | - |
dc.identifier.citation | 1.Schwarz, L. A., & Luo, L. (2015). Organization of the locus coeruleus-norepinephrine system. Current biology: CB, 25(21), R1051–R1056.
2.Aston-Jones, G., & Waterhouse, B. (2016). Locus coeruleus: From global projection system to adaptive regulation of behavior. Brain research, 1645, 75–78. 3.Aston-Jones, G., et al. (1986). The Brain Nucleus Locus Coeruleus: Restricted Afferent Control of a Broad Efferent Network. Science, 234,734-737 4.Loughlin, S. E., Foote, S. L., & Grzanna, R. (1986). Efferent projections of nucleus locus coeruleus: morphologic subpopulations have different efferent targets. Neuroscience, 18(2), 307–319 5.Kebschull, J. M., Garcia da Silva, P., Reid, A. P., Peikon, I. D., Albeanu, D. F., & Zador, A. M. (2016). High-Throughput Mapping of Single-Neuron Projections by Sequencing of Barcoded RNA. Neuron, 91(5), 975–987. 6.Hirschberg, S., Li, Y., Randall, A., Kremer, E. J., & Pickering, A. E. (2017). Functional dichotomy in spinal- vs prefrontal-projecting locus coeruleus modules splits descending noradrenergic analgesia from ascending aversion and anxiety in rats. eLife, 6, e29808. 7.Chandler, D. J., Gao, W. J., & Waterhouse, B. D. (2014). Heterogeneous organization of the locus coeruleus projections to prefrontal and motor cortices. Proceedings of the National Academy of Sciences of the United States of America, 111(18), 6816–6821. 8.Uematsu, A., Tan, B. Z., Ycu, E. A., Cuevas, J. S., Koivumaa, J., Junyent, F., Kremer, E. J., Witten, I. B., Deisseroth, K., & Johansen, J. P. (2017). Modular organization of the brainstem noradrenaline system coordinates opposing learning states. Nature neuroscience, 20(11), 1602–1611. 9.Plummer, N. W., Chandler, D. J., Powell, J. M., Scappini, E. L., Waterhouse, B. D., & Jensen, P. (2020). An Intersectional Viral-Genetic Method for Fluorescent Tracing of Axon Collaterals Reveals Details of Noradrenergic Locus Coeruleus Structure. eNeuro, 7(3), ENEURO.0010-20.2020. 10.McCall JG et al. (2017) Locus coeruleus to basolateral amygdala noradrenergic projections promote anxiety-like behavior. eLife 6, e18247. 11.Mueller D, Porter JT, Quirk GJ (2008) Noradrenergic signaling in infralimbic cortex increases cell excitability and strengthens memory for fear extinction. J Neurosci 28, 369-75. 12.Van Groen T, Wyss J M (1990) Connections of the retrosplenial granular a cortex in the rat. J Comp Neurol 300, 593-606. 13.Van Groen T, Wyss JM (1992) Connections of the retrosplenial dysgranular cortex in the rat. J Comp Neurol 315, 200-16. 14.Van Groen T, Wyss JM (2003) Connections of the retrosplenial granular b cortex in the rat. J Comp Neurol 463, 249–63. 15.Kobayashi Y, Amaral DG (2007) Macaque monkey retrosplenial cortex: III. Cortical efferents J Comp Neurol 502, 810-33. 16.Vann SD, Aggleton JP, Maguire EA (2009) What does the retrosplenial cortex do? Nat Rev Neurosci 10, 792-802. 17.Jones BF, Groenewegen HJ, Witter MP (2005) Intrinsic connections of the cingulate cortex in the rat suggest the existence of multiple functionally segregated networks. Neuroscience 133, 193–207. 18.Vogt BA, Miller MW (1983) Cortical connections between rat cingulate cortex and visual, motor, and postsubicular cortices. J Comp Neurol 216, 192–210. 19.Usher M, Cohen JD, Servan-Schreiber D, Rajkowski J, Aston-Jones G (1999) The role of locus coeruleus in regulation of cognitive performance. Science 283, 549-54. 20.Aston-Jones G, Cohen JD (2005) An integrative theory of locus coeruleus-norepinephrine function: adaptive gain and optimal performance. Annu Rev Neurosci 28, 403-50. | - |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/92195 | - |
dc.description.abstract | 中文摘要
藍斑核(LC)是哺乳動物大腦中去甲腎上腺素的主要合成中心,調節清醒、注意、壓力和高階認知控制。儘管在調節大腦功能方面起著關鍵作用,但形態學上從LC到腦皮質的連接模式仍然難以解釋。儘管最近的研究通過電生理學和RNAseq揭示了LC內的一種假設的模塊化組織,但由於先前的研究標記了大量LC神經元,因此形態學上很難區分在皮質中的單個去甲腎上腺素纖維的來源。 為了解決這個問題,我們的策略是標記少量的LC神經元並檢查它們在皮質中的投射。我們的方法是在前額葉皮質(mPFC)注射攜帶重組酶的逆行病毒,同時在LC注射攜帶重組酶依賴的螢光示踪劑的病毒。通過這種方法,我們成功地追踪了少數LC神經元,通常少於10個細胞,顯示它們不僅投射到mPFC,還在多個皮質區域中呈現廣泛和特定的投射混合。藉由其投射的模式,我們假設這兩個特定的投射代表基於它們的功能的兩種模塊化組織之一,一個用於空間識別,另一個用於運動控制。這些觀察結果提供了形態學上的證據,支持藍斑核模塊化組織的存在,並對藍斑核功能帶來了新的見解。 | zh_TW |
dc.description.abstract | Abstract
The Locus Coeruleus (LC) is the primary synthesis center of norepinephrine in the mammalian brain, modulating wakefulness, attention, stress, and higher-order cognitive control. Despite its pivotal role in brain function modulation, the connection pattern from the LC to the brain cortex has remained elusive. While recent research has proposed a hypothetical modular organization within the LC through electrophysiology and RNAseq, this hypothesis remains unverified morphologically due to previous studies labeling a large number of LC neurons, making it challenging to distinguish the origin of an individual norepinephrine fiber in the cortex from the LC. To address this question, we aimed to label a small number of LC neurons and examine their cortical projections. Our strategy involved injecting a retrograde virus carrying recombinase into the medial Prefrontal Cortex (mPFC) along with a virus carrying recombinase-dependent fluorescent tracer into the LC. Through this approach, we successfully traced a few LC neurons, typically fewer than 10 cells, revealing projections not only to the mPFC but also a mix of broad-range and specific projections in multiple cortical regions. Based on such observation, we hypothesize that these two specific projections represent two modular organizations based on their functions—one for spatial recognition and another for motor control. These observations provide morphological evidence supporting the existence of LC modular organization and shed new light on LC function. | en |
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dc.description.tableofcontents | 口試委員會審定書……………………………………………………………………i
致謝 …………….……………………………………………………………………ii 中文摘要……………………………………………………………………………..iii Abstract……………………………………………………………………………….iv Introduction ................................................................................................................ 1 1.1 Background ........................................................................................................................... 1 1.2 The Unsolved Question and Study Purpose ......................................................................... 4 1.3 Experimental Design and the Rationale ............................................................................... 4 1.4 Specific Aim ......................................................................................................................... 6 Material and Method .................................................................................................. 7 2.1 Animals: ................................................................................................................................ 7 2.2 Stereotaxic surgery for viral genetic tracing ......................................................................... 7 2.3 Tissue Collection: ................................................................................................................. 8 2.4 Immunohistochemistry (IHC):.............................................................................................. 8 2.4.1. Examination of brainstem section for validating GFP-IR signal in the LC .......................................8 2.4.2. Examination of cortical sections for validating GFP-IR signal in the AAV infusion site. .................9 2.4.3. Examination of cortex sections for confirming that GFP-IR fibers are from the LC .........................9 2.4.4. IHC for tracing of all axon co-laterals in the cortex of traced LC neurons by retrograde AAV ....... 10 2.5 Imaging and processing: ..................................................................................................... 11 Results ..................................................................................................................... 12 3.1 Tracing of axon collaterals of LC-NE neurons projecting to mPFC or S1 ........................ 12 3.2 The modified TrAC sparsely labelled LC neuron projecting to the mPFC (or S1) ............ 12 3.3 TH-IR and GFP-IR neurons in LC with rAAV infused to the mPFC ................................ 14 3.4 Distribution of axon collaterals of LC-NE neurons projecting to the mPFC ..................... 14 3.5 TH-IR and GFP-IR neurons in LC with rAAV infused to the S1 ...................................... 16 Discussion ................................................................................................................ 18 4.1 Double AAV Injection TrAC: Achieving Precision in Tracing LC-NE Neurons .............. 18 4.2 Tracing of LC-NE module possibly in volved in coordination of Spatial Recognition ..... 19 4.3 The Labelled LC-NA Modules in Mouse #543: Possibly Coordinating 2-AFC Test ........ 20 4.4 Conclusions......................................................................................................................... 21 References................................................................................................................ 22 Figures ..................................................................................................................... 24 Figure 1. Viral genetic tracing method model and the predicted tracing pattern. .................... 24 Figure 2. The injection site of mPFC and the injectionsite fraction of mPFC ......................... 26 Figure 3. The injection site of S1.............................................................................................. 27 Figure 4. The comparison of TH-IR / non-TH-IR cell numbers and the distribution of Ipsilateral GFP-IR and TH-IR cells .......................................................................................... 29 Figure 5. The distribution of Ipsilateral and Contralateral GFP-IR and TH-IR cells in #513 .. 30 Figure 6. The distribution of Ipsilateral and Contralateral GFP-IR and TH-IR cells in #544 .. 31 Figure 7. The distribution of Ipsilateral GFP-IR and TH-IR cells in #543 .............................. 32 Figure 8. The distribution of Ipsilateral GFP-IR and TH-IR cells in #159 .............................. 33 Figure 9. The distribution of Ipsilateral GFP-IR and TH-IR cells in #610 .............................. 35 Figure 10. The distribution of Ipsilateral GFP-IR and TH-IR cells in #743 ............................ 36 Figure 11. The distribution of Ipsilateral GFP-IR and TH-IR cells ......................................... 37 Figure 12. Verification of GFP-IR fibers in cortex .................................................................. 38 Figure 13. Distributions of GFP-IR fibers projection in cortex................................................ 39 Figure 14. Distributions of GFP-IR fibers projection in cortex under DAB staining .............. 40 Figure 15. The fibers distribution in #544 ................................................................................ 45 Figure 16. The normalized fibers distribution by the proportion of fiber length ..................... 46 Figure 17. The observed and hypothesized module organization of LC-NE neurons projection .................................................................................................................................................. 47 Tables ...................................................................................................................... 48 Table 1. The TH+/TH- and Ipsi/Contralateral GFP-IR neurons in LC for all 6 mice .............. 48 Table 2. The TH+/TH- and Ipsi/Contralateral GFP-IR neurons in LC for 4 qualified mice .... 48 Table 3. The quantified GFP-IR fibers distribution of #513 .................................................... 49 Table 4. The quantified GFP-IR fibers distribution of #544 .................................................... 49 Table 5. The quantified GFP-IR fibers distribution of #543 .................................................... 50 Table 6. The quantified GFP-IR fibers distribution of #159 .................................................... 50 Table 7. Tiered GFP-IR fibers distribution of all 4 mice ......................................................... 51 | - |
dc.language.iso | en | - |
dc.title | 運用基因病毒追蹤方法進行藍斑核傳出前額葉皮質的連結視覺化 | zh_TW |
dc.title | Topographic Mapping of the Locus Coeruleus Efferents to the Prefrontal Cortex Using Genetic Viral Tracing Method | en |
dc.type | Thesis | - |
dc.date.schoolyear | 112-1 | - |
dc.description.degree | 碩士 | - |
dc.contributor.coadvisor | 楊琇雯 | zh_TW |
dc.contributor.coadvisor | Hsiu-Wen Yang | en |
dc.contributor.oralexamcommittee | 陳示國;吳玉威;徐經倫 | zh_TW |
dc.contributor.oralexamcommittee | Shih-Kuo Chen;Yu-Wei Wu;Ching-Lung Hsu | en |
dc.subject.keyword | 藍斑核,基因病毒追蹤,前額葉,體感覺皮質,連結視覺化,小鼠,突觸追蹤, | zh_TW |
dc.subject.keyword | Locus Coeruleus,Virogenetic Tracing,Prefrontal Cortex,Somatosensory Cortex,Topographic Mappic,Mice,Axonal Tracing, | en |
dc.relation.page | 51 | - |
dc.identifier.doi | 10.6342/NTU202400596 | - |
dc.rights.note | 同意授權(全球公開) | - |
dc.date.accepted | 2024-02-17 | - |
dc.contributor.author-college | 生命科學院 | - |
dc.contributor.author-dept | 生命科學系 | - |
顯示於系所單位: | 生命科學系 |
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