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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 童心欣(Hsin-Hsin Tung) | |
| dc.contributor.author | Shin-Chun Ting | en |
| dc.contributor.author | 丁士鈞 | zh_TW |
| dc.date.accessioned | 2021-05-20T20:13:24Z | - |
| dc.date.available | 2009-07-27 | |
| dc.date.available | 2021-05-20T20:13:24Z | - |
| dc.date.copyright | 2009-07-27 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-07-23 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9218 | - |
| dc.description.abstract | 飲用水在處理過程中，為了去除水中之病原菌，多採用含氯消毒劑進行消毒。而含氯消毒劑會與水中固有之天然有機物(nature organic matters;NOMs)反應，產生各樣的消毒副產物(disinfection by-products;DBPs)；其中又以三鹵甲烷(Trihalomethanes;THMs)與含鹵乙酸(Haloacetic acids;HAAs)兩者為最大宗之消毒副產物。鹵乙酸經由動物實驗證實，會影響肝功能，同樣對成長、生育有極大的影響。更多研究也顯示，鹵乙酸具有致畸與致癌性。
研究指出，鹵乙酸於淨水流程中之變化可能與生物降解有關(Rodriguez et al. 2004)。而本研究針對台灣南部，具有較高鹵乙酸含量之某淨水廠，於不同季節檢測該水廠三種含氯鹵乙酸(一氯乙酸、二氯乙酸、三氯乙酸)含量，發現鹵乙酸好生成於夏季，並經過該水廠之快濾單元處理後明顯下降。本研究欲證實快濾池內附著於濾砂之微生物具有降低鹵乙酸之功效，選用變性膠體凝膠電泳(Denature Gradient Gel Electrophoresis)此種分子生物技術探討該淨水流程中微生物菌群結構的變化，並分析快濾砂上主要菌種，以期建立鹵乙酸濃度與實廠內微生物菌群之關連性。 | zh_TW |
| dc.description.abstract | In order to reduce the human health impacts due to pathogens in drinking waters, it is important to apply disinfection before distribution. Disinfectants widely used nowadays are chlorine or chloramides. These disinfectants react with nature organic matters in water, and produce disinfection by-products (DBPs).Trihalomethanes (THMs) and Haloacetic acids (HAAs) are two of the DBPs with highest amount by weight from chlorination. HAAs affect to influence hepatic, reproductive, and developmental functions possibly, and they may be mutagenic and carcinogenic in animal studies.
Previous studies had showed that HAA biodegradation could happen during water treatment process. This study aimed at one of the water treatment plant in southern Taiwan which had high HAA concentrations and investigated the changes of three HAAs (MCAA、DCAA and TCAA) during treatment process at different seasons. The results showed that HAAs were produced mostly at summer, and could be reduced significantly after passing through the rapid sand filtration process. It is considered to be connected with biodegradation upon filtration sands. This study used DGGE to explore the microbial’s population in each treatment process, as well as analyzing the main bacterial species in filtration sands, and this result could help us to understand microbial communities in water treatment process. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T20:13:24Z (GMT). No. of bitstreams: 1 ntu-98-R95541105-1.pdf: 946545 bytes, checksum: 628d1fc60be6d25b5cb393f3ced47649 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 目錄
摘要 I ABSTRACT II 目錄 III 圖目錄 V 表目錄 VI 附錄 VII 第一章 前言 1 1-1 研究背景 1 1-2 研究目的 3 第二章 文獻回顧 4 2-1 消毒副產物(DISINFECTION BY-PRODUCT) 4 2-2 含鹵乙酸(HALOACETIC ACIDS) 8 2-3 影響鹵乙酸生成因子 10 1.消毒劑種類 10 2.酸鹼度(pH) 10 3.天然有機物(Nature organic materials；NOMs) 11 4.溫度 12 5.消毒劑接觸時間 13 6.溴離子 14 2-4 含鹵乙酸與微生物之關係 15 2-5 本研究相關分子生物技術簡介 18 1.核醣體與16SrRNA gene 18 2.聚合酶鏈鎖反應(Polymerase chain reaction) 20 3.變性梯度凝膠電泳（denaturing gradient gel electrophoresis；DGGE） 23 2-6 分子生物技術於環境上之應用與優缺點。 25 第三章 材料與方法 27 3-1全流程一氯、二氯、三氯乙酸濃度檢測 27 I.藥品材料 27 II.儀器設備 28 III.步驟方法 29 3-2一氯、二氯、三氯乙酸濾砂降解試驗 30 I.藥品材料 30 II.儀器設備 30 III.步驟方法 31 3-3全流程單元生物膜與水體微生物菌群分析 33 I.藥品材料 33 微生物核酸萃取用藥品 33 變性梯度凝膠電泳藥品 34 II.儀器設備 34 III.步驟方法 34 （1）微生物DNA萃取及純化實驗 34 （2）引子(primer)的選用 35 （3）聚合酶鏈鎖反應(Polymerase chain reaction；PCR) 36 （4）變性梯度凝膠電泳(DGGE) 36 （5）染色：銀染 (Silver staining)與SYBR Green I 36 （6）核酸序列結果分析 43 （7）圖譜分析 44 第四章 結果與討論 46 4-1一氯、二氯、三氯乙酸於自來水廠處理單元之變化。46 4-2快氯砂降解一氯、二氯、三氯乙酸批次試驗。50 4-3 水廠處理單元之生物膜及水體之菌相與菌種分析。57 4-3-1 池壁生物膜與濾砂PCR-DGGE菌相之探討。59 4-3-2 各單元水體微生物PCR-DGGE菌相之探討。62 4-3-3以16SrDNA鑑定附著於快濾砂之微生物菌種。69 4-3-4 HAA降解後，濾砂菌群之變化。 71 第五章 結論與建議 72 5-1 結論72 5-2 建議74 第六章 參考文獻 77 附錄 81 | |
| dc.language.iso | zh-TW | |
| dc.title | 含氯鹵乙酸於飲用水淨水流程中之生物降解作用研究與探討 | zh_TW |
| dc.title | Investigation of Chlorinated Haloacetic Acids Biodegradation in Drinking Water Treatment Process | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王根樹(Gen-Shuh Wang),張靜文(CHING-WEN CHANG) | |
| dc.subject.keyword | 鹵乙酸,生物降解,快濾砂,DGGE,淨水流程, | zh_TW |
| dc.subject.keyword | Haloacetic acids,Biodegradation,Rapid sand filtration,DGGE,Water Treatment, | en |
| dc.relation.page | 86 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2009-07-23 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 環境工程學研究所 | zh_TW |
| 顯示於系所單位： | 環境工程學研究所 | |
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