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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91715
標題: | 番茄重組自交族群 CLN4220 果實產量相關性狀變異的遺傳組成分析 Analysis of the Genetic Composition for Phenotypic Variation of Fruit Yield-related Traits in the Tomato Recombinant Inbred Population CLN4220 |
作者: | 林宥任 Yu-Jen Lin |
指導教授: | 陳凱儀 Kai-Yi Cheng |
關鍵字: | 番茄,熱逆境,花朵,果實,產量相關性狀,數量性狀基因座, Tomato,Heat stress,Flower,Fruit,Yield-related traits,Quantitative trait loci, |
出版年 : | 2024 |
學位: | 碩士 |
摘要: | 高溫是影響番茄生產的主要逆境因子,尤其在台灣的夏季造成嚴重的產量損失。隨著全球平均氣溫的逐漸升高,對於番茄耐熱性的育種研究也愈發重要。番茄的花與果實容易受到高溫的影響,造成花朵數、果實數、結果率等產量相關性狀的下降,最終導致產量的損失。為了探討長期和緩高溫影響產量相關性狀背後的遺傳高溫與常溫試驗、調查外表型變異、並進行數量性狀基因座定位。外表型調查的結果顯示長期和緩高溫無論在田間或溫室都造成花序上果實數量與結果率的下降。在高溫下,結果率與果實數的相關性比常溫時更加緊密,且花朵數與果實數的相關性降低。數量性狀基因座分析的結果顯示在 1 號染色體 51.6 cM 至 69 cM 處存在一個在所有環境下皆與花朵數相關的基因座,並在2、3、4、5、6、7 與 12 號染色體上也定位到各種產量相關性狀的基因座。本研究的結果展示了番茄重組自交族群CLN4220產量相關性狀在不同栽培設施下對高溫的反應,並發現許多與這些性狀相關的遺傳因子,這些都是後續進行番茄耐熱育種研究時重要的參考資料。 High temperature poses a significant challenge to tomato production, particularly during the summer months in Taiwan. Research on heat tolerance in tomatoes has become increasingly important as global temperatures rise. Under high-temperature conditions, tomato flowers and fruits are particularly vulnerable, leading to reduced yield-related traits such as flower number, fruit number, and fruit set, ultimately resulting in yield loss. To investigate the genetic composition underlying the effects of long-term mild heat on yield-related traits, a recombinant inbred population, CLN4220, was used to study phenotypic variation and map quantitative trait locus in both field and greenhouse settings under high and ambient temperatures. The phenotypic results showed that long-term mild heat caused a decrease in the fruit number and the fruit set on the inflorescence in both field and greenhouse conditions. The correlation between fruit set and fruit number was stronger at higher temperatures than ambient temperatures, while the correlation between flower number and fruit number was reduced. Eventually, the quantitative trait locus analysis identified a locus associated with flower number in all environments at 51.6cM to 69cM of chromosome 1. Additionally, loci for various yield-related traits were found on chromosomes 2, 3, 4, 5, 6, 7, and 12. These findings provide insights into the response of tomato yield-related traits to high temperatures in different cultivation facilities and reveal the genetic factors controlling these traits. This information will be valuable for future research on heat tolerance breeding in tomatoes. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91715 |
DOI: | 10.6342/NTU202400382 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 農藝學系 |
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