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???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
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dc.contributor.advisor | 林?輝 | |
dc.contributor.author | Chun-han Hou | en |
dc.contributor.author | 侯君翰 | zh_TW |
dc.date.accessioned | 2021-05-20T20:11:23Z | - |
dc.date.available | 2014-08-03 | |
dc.date.available | 2021-05-20T20:11:23Z | - |
dc.date.copyright | 2009-08-03 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-07-28 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9162 | - |
dc.description.abstract | 腫瘤熱治療是目前非常熱門的研究題目,而許多新型的磁性奈米粒子也被研發出來,作為熱治療的熱種子(thermal seeds)。在本論文的第一部份,我們嘗試以氫氧基磷灰石(hydroxyapatite)為基底之磁性奈米粒子(magnetic hydroxyapatite nanoparticles,簡稱 mHAP)做為熱種子,注射入小鼠皮下腫瘤附近。結果於15天的觀察期內,只有注射mHAP及置入高頻交互磁場之小鼠,其腫瘤會在五天內迅速縮小。並且老鼠犧牲後之血清測試證明mHAP之毒性甚低。
第二部分則是以雙水雙鈣磷酸鹽dicalcium phosphate dihydrate (DCPD)為基底合成新的磁性奈米粒子(mDCPD),隨著不同的鐵含量被加入,其所表現的物理化學性質皆有顯著的不同,可以用X光散射 x-ray diffraction (XRD), 感應耦合電漿發射光譜分析儀inductively coupled plasma-optical emission spectrometer (ICP-OES), 超導量子干涉儀superconducting quantum interference device (SQUID), 和穿透式電子顯微鏡transmission electron microscopy (TEM)來檢查。加熱效率也在可接受之範圍內。細胞毒性測試(WST-1 and LDH assay)亦顯示其無毒性。在體外試驗中,mDCPD能顯著地殺死癌細胞而不損傷正常細胞。 | zh_TW |
dc.description.abstract | Hyperthermia therapy for cancer has drawn more and more attention these days. Many different types of magnetic particles have been developed for the purpose of hyperthermia cancer therapy. In the first part, we conducted an in vivo cancer hyperthermia study of the new magnetic hydroxyapatite nanoparticles (mHAP) on a mouse model. Only the mice which were injected with mHAP and had been treated inside the magnetic field showed dramatic reduction of tumor volume, in the 15-day observation period. No local recurrence was noted. Therefore, our new magnetic hydroxyapatite nanoparticles have demonstrated therapeutic effect in a mouse model with little toxicity.
In the second part, a magnetic nanoparticle based on dicalcium phosphate dihydrate (DCPD) was formed by co-precipitation method. Addition of different concentrations of ferrous chloride to DCPD can alter its material properties. Various physical, chemical and magnetic tests of the magnetic DCPD nanoparticles (mDCPD) were performed, including x-ray diffraction (XRD), inductively coupled plasma-optical emission spectrometer (ICP-OES), superconducting quantum interference device (SQUID), and transmission electron microscopy (TEM). The heating efficiency of mDCPD in alternating magnetic field was proved to be suitable for hyperthermia. The results of cytotoxicity tests (WST-1 and LDH assay) showed no harmful effect. The mDCPD showed relative cancer-killing ability without damaging normal cells in vitro. | en |
dc.description.provenance | Made available in DSpace on 2021-05-20T20:11:23Z (GMT). No. of bitstreams: 1 ntu-98-D95548016-1.pdf: 3626252 bytes, checksum: c126624663704267e4436c8774187248 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | Contents
致謝 I Abstract III 摘要 IV Contents V List of tables IX List of figures X Chapter 1 Introduction - 1 - 1.1 Prologue - 1 - 1.2 Cancer - 3 - 1.3 Cancer therapy - 5 - 1.3.1 Surgery - 5 - 1.3.2 Radiation - 6 - 1.3.3 Chemotherapy - 8 - 1.3.4 Immunotherapy - 11 - 1.3.5 Gene therapy - 12 - 1.3.6 Hyperthermia - 14 - 1.4 Comparisons of cancer therapies - 16 - 1.5 Mechanism of hyperthermia - 18 - 1.6 Classification of hyperthermia by heating method - 24 - 1.7 Magnetically mediated hyperthermia (MMH) - 26 - 1.7.1 Background - 26 - 1.7.2 Definition - 27 - 1.7.3 Arterial embolization hyperthermia (AEH) - 28 - 1.7.4 Direct injection hyperthermia (DIH) - 30 - 1.7.5 Interstitial implant hyperthermia (IIH) - 32 - 1.7.6 Intracellular hyperthermia (IH) - 34 - 1.7.7 The future - magnetic nanoparticles - 37 - 1.7.8 Comparison of magnetically mediated hyperthermia - 38 - 1.8 Magnetic nanoparticles for biomedical applications - 41 - 1.8.1 Particle size - 41 - 1.8.2 Biomedical applications of magnetic particles - 43 - 1.9 Drug delivery system for hyperthermia - 47 - 1.10 Purpose of the study - 49 - Chapter 2 Experiment settings - 50 - 2.1 Experiment equipments - 50 - 2.2 Reagents - 52 - 2.3 X-ray diffractometer (XRD) - 54 - 2.4 Scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDS) - 55 - 2.5 Superconducting quantum interference device (SQUID) - 56 - 2.6 Transmission electron microscopy (TEM) - 57 - 2.7 Biocompatibility - 60 - 2.7.1 Lactate dehydrogenase (LDH) assay - 60 - 2.7.2 Water-soluble tetrazolium salt-1 (WST-1) assay - 61 - Chapter 3 Hydroxyapatite -modified biomagnetic nanoparticles - 63 - 3.1 Introduction - 63 - 3.2 Materials & Methods - 66 - 3.2.1 Preparation of hydroxyapatite (HAP) and magnetic-HAP(m-HAP) nanoparticles - 66 - 3.2.2 Heating efficiency (in vivo) - 66 - 3.2.3 Animal study - 69 - 3.3 Results - 70 - 3.4 Discussion - 77 - Chapter 4 Dicalcium phosphate dihydrate (DCPD)- modified biomagnetic nanoparticles - 80 - 4.1 Introduction - 80 - 4.2 Material & Methods - 82 - 4.2.1 DCPD synthesis - 82 - 4.2.2 mDCPD synthesis - 82 - 4.2.3 X-ray diffraction (XRD) - 83 - 4.2.4 Chemical composition (ICP-OES) - 83 - 4.2.5 Magnetic property - 83 - 4.2.6 Inductive heater and temperature recording - 84 - 4.2.7 Particle size - 85 - 4.2.8 The WST-1 assay for mitochondrial function - 85 - 4.2.9 Lactate dehydrogenase (LDH) assay for cell lysis - 86 - 4.2.10 In-vitro test for cancer hyperthermia - 86 - 4.3 Results - 88 - 4.3.1 XRD pattern - 88 - 4.3.2 Lattice parameters - 89 - 4.3.3 Chemical composition (ICP-OES) - 91 - 4.3.4 Magnetic property - 91 - 4.3.5 Heating efficiency - 93 - 4.3.6 Particle size (TEM) - 94 - 4.3.7 WST-1 assay for mitochondrial function - 95 - 4.3.8 LDH assay for cell lysis - 96 - 4.3.9 In-vitro test for cancer hyperthermia - 97 - 4.4 Discussion - 99 - Chapter 5 Conclusions - 102 - Chapter 6 References - 105 - Chapter 7 Appendix - 121 - 7.1 Curriculum Vitae of the author (中文) - 121 - 7.2 Curriculum Vitae of the author (English) - 123 - 7.3 Refereed papers published - 125 - 7.4 Conference papers - 127 - 7.5 Other publications - 130 - | |
dc.language.iso | en | |
dc.title | 合成磷酸鈣磁性奈米粒子於癌症熱療之應用 | zh_TW |
dc.title | Calcium Phosphate-Based Magnetic Nanoparticles as Thermoseeds for Cancer Hyperthermia Therapy | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 劉華昌,楊榮森,劉振軒,許淙慶 | |
dc.subject.keyword | 磁性奈米粒子,癌症,腫瘤熱治療,氫氧基燐灰石,雙水雙鈣磷酸鹽, | zh_TW |
dc.subject.keyword | magnetic nanoparticles,cancer,hyperthermia,hydroxyapatite,dicalcium phosphate dehydrate, | en |
dc.relation.page | 130 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2009-07-28 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
Appears in Collections: | 醫學工程學研究所 |
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ntu-98-1.pdf | 3.54 MB | Adobe PDF | View/Open |
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