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標題: | 基於石墨烯的納米架構可持續絲網印刷生物傳感平台用於護理點診斷 Graphene-based nano-architectured sustainable screen-printed biosensing platforms for point-of-care-diagnostics |
作者: | 司答林 STALIN KARUPPIAH |
指導教授: | 周家復 Chia-Fu Chou |
關鍵字: | 石墨烯,點診斷,COVID-19,通過快速電化學檢測進行, Graphene,SPE,COVID-19,Biosensor,Electrochemical, |
出版年 : | 2023 |
學位: | 博士 |
摘要: | 考慮到全球疾病負擔和死亡率,無法獲得診斷對於疾病的生存起著重要作用。 因此,現在是為面臨水傳播疾病和其他傳染病等各種發展問題的低收入和中等收入國家(LMIC)創建最終用戶和護理點診斷工具的時候了。 我們報告了一種可持續的生物分析設備平台,即生物傳感器,用於檢測水污染和 COVID-19 致病病毒。 SARS-CoV-2. 該生物傳感器採用絲網印刷方法製造,採用可生物降解的基材,如紙張、PET等,我們製造的生物傳感器由絲網印刷石墨烯上的氧化石墨烯組成,石墨烯是迄今為止發現的突破性二維材料之一,作為工作電極。 電化學家對半導體以外的二維石墨烯基材料的看法,推動將它們用於電化學生物傳感器,例如快速電子轉移動力學、電學和光學可調特性、易於使用的生物共軛策略、優異的電化學(例如:高法拉第與電容電流比) ,電子遷移率,高電流密度)。
(我)。 用於監測水性細菌污染的超靈敏且低成本的紙基氧化石墨烯納米生物傳感器 水傳播的病原體大多是由於衛生條件差、工業廢水和污水污泥產生的,導致死亡率顯著增加。 為了防止這種情況發生,我們需要一種簡單、用戶友好、快速的病原體現場檢測工具,即生物傳感器。 由於受污染的水主要含有(80%)大腸菌,其中大腸桿菌是主要菌種,我們開發了一種絲網印刷紙基、無標籤生物傳感器,用於檢測水中的大腸桿菌。 將納米結構的氧化石墨烯(GO) 作為快速電子轉移平面沉積在疏水紙上的絲網印刷石墨烯(G) 上,然後固定凝集素伴刀豆球蛋白A (ConA) 作為GGO_ConA 生物傳感的生物識別元件電極。 GGO_ConA 的電化學表徵顯示出快速電子轉移,計算出的電活性表面積為 0.16 cm2。 使用電化學阻抗譜(EIS)技術以污泥水和海灘水(真實樣品)作為分析物測試生物傳感器的性能。 GGO_ConA 的電荷轉移電阻 (Rct) 在 10−108 CFU mL−1 範圍內隨細菌濃度線性增加,估計檢測限 (LOD) 為 10 CFU mL−1,這表明我們的生物傳感器具有超靈敏性,靈敏度比之前的研究高出100 倍。 我們報導的生物傳感器具有成本效益、環保且超靈敏,可以極大地用作檢查水傳播細菌污染的便攜式監測套件。 (二). 通過快速電化學檢測進行 COVID-19 篩查以及通過便攜式電分析設備對 SARS-CoV-2 進行定量 最近出現的冠狀病毒病 COVID-19 可引起嚴重急性呼吸綜合徵冠狀病毒 2 (SARS-CoV-2),導致嚴重呼吸困難和肺炎。 這種疾病的極端傳染性和感染人數促使世界衛生組織將這次疫情列為大流行病。 由於向不發達國家和人口稠密的國家提供疫苗變得很困難,臨床醫生只能依靠對 COVID-19 患者的早期診斷、隔離和治療。 在這裡,我們首次報告了一種檢測 SARS-CoV-2 的電化學轉導方法。 開發了一種新型絲網印刷免疫傳感器,用於快速檢測和定量SARS-CoV-2 病毒刺突蛋白,採用單層氧化石墨烯固定有與不同濃度的SARS-CoV-2 刺突蛋白結合的抗SARS-CoV-2。 使用差分脈衝伏安法(DPV)在裂解緩衝液中測試所製造的免疫傳感器的分析性能。 結果顯示檢測限約為 41.3 pg/ml,很有前景。 因此,我們開發了一種基於絲網印刷方法的新型生物傳感器,用於檢測和定量 SARS-CoV-2,具有卓越的靈敏度、特異性和快速檢測能力。 Considering global burden of diseases and mortality rate, inaccessibility to the diagnosis plays a major role for the ailments to live. Hence, it is high time to create an end-user and point-of-care diagnostic tools for low- and middle-income countries (LMICs) which are having various development issues such as water-borne diseases, and other communicable diseases. We reported a sustainable bioanalytical device platform, i.e. biosensor, to detect water contamination and COVID-19 causative virus. SARS-CoV-2. The biosensor was fabricated by screen-printing method, employed bio-degradable substrates such as paper, PET etc., Our fabricated biosensor consists of the graphene oxide on screen-printed graphene, one of the ground-breaking 2D materials ever discovered, as working electrode. An electrochemist view of 2D graphene-based materials beyond semiconductors, drives to utilize them into electrochemical biosensors, such as fast electron transfer kinetics, both electrically and optically tunable properties, easily available bioconjugation strategies, excellent electrochemistry (e.g: high Faradaic to capacitive current ratios, electron mobility, high current density). (i). Ultrasensitive and Low-Cost Paper-Based Graphene Oxide Nanobiosensor for Monitoring Water-Borne Bacterial Contamination Water-borne pathogens are mostly generated due to poor sanitation, industrial effluents, and sewage sludge, leading to a significant increase in mortality rate. To prevent this, we need a simple, user-friendly, and rapid on-site detection tool of pathogens, i.e., a biosensor. As contaminated water mainly contains (80%) coliform bacteria, of which Escherichia coli is the major species, we have developed a screen-printed paper-based, label-free biosensor for the detection of E. coli in water. A nanoarchitectured graphene oxide (GO), as a fast electron-transfer flatland, was deposited on the screen-printed graphene (G) on a hydrophobic paper, followed by the immobilization of lectin Concanavalin A (ConA) as a biorecognition element for a GGO_ConA-biosensing electrode. The electrochemical characterization of GGO_ConA shows fast electron transfer with a calculated electroactive surface area of 0.16 cm2. The biosensor performance was tested in the sludge water andbeach water (real sample) as an analyte using the electrochemical impedance spectroscopy (EIS) technique. The charge-transfer resistance (Rct) of GGO_ConA increases linearly with the bacterial concentration in the range of 10−108 CFU mL−1 with an estimated limit of detection (LOD) of 10 CFU mL−1, which indicates the ultrasensitivity of our biosensor, with 100 times more sensitivity than previous studies. Our reported biosensor, being cost-effective, eco-friendly, and ultrasensitive, may serve greatly as portable monitoring kit for checking water-borne bacterial contamination. (ii). COVID-19 Screening by Rapid Electrochemical Detection and Quantification of SARS-CoV-2 by Portable Electroanalytical Device The recently emerged coronavirus disease, COVID-19, can cause severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which leads to severe distress in breathing and pneumonia. The extreme contagiousness and number infections due to this disease has prompted the World Health Organization to classify this outbreak as a pandemic. As the availability of the vaccines to underdeveloped and densely populated countries became cumbersome, clinicians have to rely only on early diagnosis, isolation and treatment of COVID-19 patients. Here, we report for the first time an electrochemical transduction method to detect SARS-CoV-2. A novel screen printed immunosensor was developed for rapid detection and quantification of SARS-CoV-2 viral spike protein, employing monolayered graphene oxide immobilized with anti-SARS-CoV-2 bound to different concentrations of the SARS-CoV-2 spike protein. The analytical performance of the fabricated immunosensor was tested in lysis buffer using differential pulse voltammetry (DPV). The results show a promising limit of detection of 41.3 pg/ml approximately. Hence, we developed a novel biosensor based on a screen-printing method to detect and quantify SARS-CoV-2 with remarkable sensitivity, specificity and rapid testing ability. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91529 |
DOI: | 10.6342/NTU202304120 |
全文授權: | 同意授權(限校園內公開) |
顯示於系所單位: | 化學系 |
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