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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 陳國勤 | zh_TW |
| dc.contributor.advisor | Benny Kwok-Kan Chan | en |
| dc.contributor.author | 李旻憲 | zh_TW |
| dc.contributor.author | Min-Hsien Lee | en |
| dc.date.accessioned | 2023-12-12T16:22:12Z | - |
| dc.date.available | 2023-12-13 | - |
| dc.date.copyright | 2023-12-12 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-10-16 | - |
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Lake, M. G. Frick, and P. D. Rawson. 2014. Deconstructing an assemblage of “turtle” barnacles: species assignments and fickle fidelity in Chelonibia. Marine Biology, 161:45 – 59. Zevina, G. B. 1982. Barnacles of the Suborder Lepadomorpha (Cirripedia, Thoracica) of the world Ocean II. Fauna SSSR (Russia), 133:1 – 223. Zhang, J., P. Kapli, P. Pavlidis, and A. Stamatakis. 2013. A general species delimitation method with applications to phylogenetic placements. Bioinformatics, 29:2869 – 2876. Zullo, V. A. 1973. A Late Miocene record of Lepas Linnaeus (Cirripedia, Lepadidae) from Southern California. In: Contributions in science, vol. 241. Natural History Museum, Los Angeles, U.S.A. pp1 – 6. Zullo, V. A. 1979. Marine flora and fauna of the northeastern United States. Arthropoda: Cirripedia. U.S. Department of Commerce, National Oceanic and Atmospheric Administration, & National Marine Fisheries Service, Washington DC, U.S.A. 29pp. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91242 | - |
| dc.description.abstract | 茗荷藤壺 (Lepas) 為海洋表層的生物 (epipelagic organism) ,牠們的成體 (adult) 附著於海洋漂流物上,此特性使得茗荷藤壺得以隨著洋流漂流而成為廣泛分佈於世界各個海洋的生物。近年來有研究以COI、16S及18S三個遺傳標記所構成的分子證據指出,茗荷藤壺的族群因為陸地阻礙、洋流流向與洋流表層水溫等地理隔閡效應的影響而分化成區域性的分支 (biogeographic clades) 。然而,西北太平洋海域卻因為只有5隻L. anserifera 採集於日本,而不能有代表性地代表西北太平洋地區的多樣性。本研究以台灣周圍海域的茗荷藤壺標本做分子與形態的綜合分析,以探討茗荷藤壺在台灣周圍海域的多樣性模式。從四個DNA條碼基因 (COI, 16S, 18S and H3) 所構成的親緣關係樹 (phylogenetic trees) 中,本研究發現在台灣茗荷藤壺共可分成四個物種及五個區域性分支 (biogeographic clades)。之前被認為只在美國奧勒岡州 (Oregon) 發現之 L. anatifera (Oregon clade) 也出現在台灣海域,故本論文改稱之為L. anatifera北太平洋分支 (North-Pacific clade)。本論文也鑑定出L. pectinata於西北太平洋的一個新的分支 (NW-Pacific clade) 與一個未知的物種Lepas sp.。L. anatifera的北太平洋分支與L. pectinata的西北太平洋分支可能是受到北太平洋環流 (North Pacific gyre) 與赤道逆流 (Equatorial Countercurrent) 局限而分化。L. anatifera的全球性分支 (Global-clade) 與L. anserifera 也同樣出現在台灣海域,而L. anserifera是唯一未分化的全球性物種 (cosmopolitan species) 。以Assemble Species by Automatic Partitioning (ASAP) 和Bayesian implementation of the Poisson Tree Processes Model (bPTP) 兩種物種界定方法做出的結果也顯示,各個物種內大多數的區性分支之間的差異可能足以視為不同物種,而綜合分子與形態分析後,本研究認為L. anatifera 北太平洋分支與全球性分支可被視為不同物種。此外,未知物種L. sp.在過往曾以外觀特徵被認為是L. testudinata。然而,本研究經由分子證據佐以對過往的文獻記錄做形態上的比對後,認為L. sp.與L. testudinata為不同物種。L. testudinata模式地 (type locality) 於南非,故分佈於南半球的物種為L. testudinata,而本論文採得於西北太平洋的物種可被視為一個未知的新物種L. sp.。 | zh_TW |
| dc.description.abstract | Gooseneck barnacles of the genus Lepas are epipelagic organisms. Their adults attach to ocean-floating objects and can be dispersed by the ocean currents. Lepas barnacles thus have worldwide distributions. Previous studies studied the diversity of Lepas using COI, 16S, and 18S genes and showed that the populations were differentiated into several biogeographic clades which are influenced by vicariance effects of continental isolation, ocean currents, and sea surface temperature (SST). However, the diversity in the Northwest Pacific was understudied because the pattern addressed was based on five L. anserifera specimens collected from Japan. In the present study, extensive Lepas specimens were collected around Taiwan and were analyzed with DNA barcoding genes (COI, 16S, 18S, and H3 genes) and morphological approaches. Compared to previous studies, our study revealed the original Oregon clade of Lepas anatifera is also present in Taiwan waters and we named it as North-Pacific clade. We have identified one more clade of L. pectinata which is distributed in the northern hemisphere of the Pacific. We also identified one unknown, probably new species of Lepas in the NW Pacific. The differentiations of L. anatifera North-Pacific clade and L. pectinata NW-Pacific clade may be caused by the limited gene flow which is affected by the North Pacific gyre and Equatorial Countercurrent. The Taiwan waters also contain the Global clade of L. anatifera and L. anserifera. Only L. anserifera is undifferentiated and is cosmopolitan in the world. The species delimitation results of Assemble Species by Automatic Partitioning (ASAP) and Bayesian Implementation of the Poisson Tree Processes Model (bPTP) also suggested most regional clades of Lepas species may be considered as separate species. According to the combination of morphological analysis and molecular analysis, the North-Pacific clade and Global clade of L. anatifera can be considered as different species. In addition, the undescribed L. sp. identified from the present study was morphologically similar to L. testudinata. However, the present study shows they are different species according to the molecular evidence and historical taxonomic records. L. testudinata was first identified in South Africa, and therefore the L. testudinata in the southern ocean represent the true L. testudinata and the species identified in the Northwest Pacific (morphologically similar to L. testudinata) can be revealed as an undescribed new species. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-12-12T16:22:12Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-12-12T16:22:12Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
Acknowledgements ii 摘要 iii Abstract v 1. Introduction 1 1.1 Biogeography and phylogeography of barnacles 1 1.2 The pelagic stalked barnacle Lepas 4 1.3 History of taxonomic research in Lepas 4 1.3.1 First identification of the genus Lepas 4 1.3.2 Subgenera of Lepas 5 1.3.3 Valid species of Lepas 7 1.3.4 Species concept and species identities of Lepas 13 1.4 Molecular phylogeny and biodiversity of Lepas 19 1.5 Aim 19 2. Materials and methods 21 2.1 Sampling and preservation of specimens 21 2.2 DNA extraction, PCR, and sequencing 21 2.3 Phylogenetic analysis 22 2.4 Species delimitation 26 2.5 Morphological analysis 27 2.5.1 Specimen groupings 27 2.5.2 Multivariate analysis 28 2.5.3 Morphology of cirri, somatic body, and mouth parts. 29 3. Results 31 3.1 Phylogenetic analysis 31 3.1.1 18S nuclear gene phylogenetic pattern 31 3.1.2 COI, 16S mitochondrial phylogenies and concatenating analysis 32 3.1.3 H3 phylogeny and concatenating analysis of sequences from Taiwan 36 3.2 Species delimitation 38 3.3 Morphological analysis 40 3.3.1 External variances 40 3.3.2 Multivariate analysis 49 3.3.3 Observation of somatic body 50 4. Discussion 64 4.1 Diversity of Lepas 64 4.1.1 Diversity of Lepas in Taiwan 64 4.1.2 Genetic diversity of Lepas 65 4.2 Taxonomic issue of Lepas in Taiwan 72 4.2.1 Global clade and North-Pacific clade of L. anatifera 72 4.2.2 L. anserifera 78 4.2.3 L. pectinata NW-Pacific clade 79 4.2.4 L. sp. 83 Conclusions 87 Reference 89 List of Figures Fig. 1. General characters of Lepas 110 Fig. 2. Sampling locations around the coastline of Taiwan and outlying islands. 111 Fig. 3. Parameters of multivariate analysis for comparing the differences in capitulum shape between between Global clade and North-Pacific clade of L. anatifera. 112 Fig. 4. 18S nuclear gene phylogeny. 113 Fig. 5. 16S mitochondrial phylogeny. 114 Fig. 6. COI and 16S combined mitochondrial phylogeny. 115 Fig. 7. Species delimitation with COI and 18S combined phylogeny. 116 Fig. 8. H3 mitochondrial phylogeny. 117 Fig. 9. COI, 16S, 18S, and H3 combined mitochondrial phylogeny. 118 Fig. 10. External variations of Global clade of L. anatifera. 119 Fig. 11. External variations of North-Pacific clade of L. anatifera. 120 Fig. 12. External variations of L. anserifera. 121 Fig. 13. External variations of L. sp. 122 Fig. 14. External variations of NW-Pacific clade of L. pectinata. 123 Fig. 15. 2-dimensional nMDS ordination plot generated from the 20 parameters displayed in Fig. 3. 124 Fig. 16. Filamentary appendages of L. anatifera Global clade 125 Fig. 17. Cirri I and II of L. anatifera Global clade 126 Fig. 18. Cirri III and IV of L. anatifera Global clade 127 Fig. 19. Cirri V and VI of L. anatifera Global clade 128 Fig. 20. Maxilla, maxillule and mandible of L. anatifera Global clade 129 Fig. 21. Mandible palp and labrum of L. anatifera Global clade 130 Fig. 22. Filamentary appendages of L. anatifera North-Pacific clade 131 Fig. 23. Cirri I and II of L. anatifera North-Pacific clade 132 Fig. 24. Cirri III and IV of L. anatifera North-Pacific clade 133 Fig. 25. Cirri V and VI of L. anatifera North-Pacific clade 134 Fig. 26. Maxilla, maxillule and mandible of L. anatifera North-Pacific clade 135 Fig. 27. Mandible palp and labrum of L. anatifera North-Pacific clade 136 Fig. 28. Filamentary appendages of L. anserifera 137 Fig. 29. Filamentary appendages of L. anserifera 138 Fig. 30. Cirri I and II of L. anserifera 139 Fig. 31. Cirri III and IV of L. anserifera 140 Fig. 32. Cirri V and VI of L. anserifera 141 Fig. 33. Maxilla, maxillule and mandible of L. anserifera. 142 Fig. 34. Mandible palp and labrum of L. anserifera 143 Fig. 35. Filamentary appendages of L. sp. 144 Fig. 36. Filamentary appendages of L. sp. 145 Fig. 37. Cirri I and II of L. sp. 146 Fig. 38. Cirri III and IV of L. sp. 147 Fig. 39. Cirri V and VI of L. sp. 148 Fig. 40. Maxilla, maxillule and mandible of L. sp. 149 Fig. 41. Mandible palp and labrum of L. sp. 150 Fig. 42. Filamentary appendages of L. pectinata NW-Pacific clade. 152 Fig. 43. Cirri I and II of L. pectinata NW-Pacific clade. 153 Fig. 44. Cirri III and IV of L. pectinata NW-Pacific clade 154 Fig. 45. Cirri V and VI of L. pectinata NW-Pacific clade 155 Fig. 46. Maxilla, maxillule and mandible of L. pectinata NW-Pacific clade. 156 Fig. 47. Mandible palp and labrum of L. pectinata NW-Pacific clade. 157 Fig. 48. Comparison of teeth numbers on mandibles of L. pectinata NW-Pacific clade. 158 Fig. 49. Comparison of setal types on distal region of Cirri I and endopodite of Cirri II in Global clade of L. anatifera. 160 Fig. 50. Comparison of setal types on distal region of Cirri I and endopodite of Cirri II in North-Pacific clade of L. anatifera. 162 Fig. 51. The updated diversity of Lepas. 163 List of Tables Table 1. Nomenclature and type status of currently valid Lepas species 164 Table 2. Valid species and molecular information 165 Table 3. Used primers and annealing temperatures 168 Table 4. List of morphotypes and quantities of used specimens in multivariate analysis 169 Table 5. Pairwise distances of COI in Lepas 170 Table 6. Results of multivariate analysis based on parameters in Fig. 3. 171 List of Appendixes Appendix 1. Sample information in the present study. 172 Appendix 2. Reference sample information from Schiffer & Herbig (2016). 186 Appendix 3. 16S phylogeny 194 Appendix 4 18S phylogeny 196 Appendix 5. H3 phylogeny 198 Appendix 6. COI+16S phylogeny 199 Appendix 7. COI+16S+18S phylogeny 201 Appendix 8. COI+16S+18S+H3 phylogeny 203 | - |
| dc.language.iso | en | - |
| dc.subject | 茗荷屬 | zh_TW |
| dc.subject | 鵝頸藤壺 | zh_TW |
| dc.subject | 茗荷屬 | zh_TW |
| dc.subject | 隱蔽種 | zh_TW |
| dc.subject | 地理隔離 | zh_TW |
| dc.subject | 親緣關係 | zh_TW |
| dc.subject | 形態 | zh_TW |
| dc.subject | 物種界定 | zh_TW |
| dc.subject | 生物地理學 | zh_TW |
| dc.subject | 生物地理學 | zh_TW |
| dc.subject | 物種界定 | zh_TW |
| dc.subject | 形態 | zh_TW |
| dc.subject | 親緣關係 | zh_TW |
| dc.subject | 地理隔離 | zh_TW |
| dc.subject | 隱蔽種 | zh_TW |
| dc.subject | 鵝頸藤壺 | zh_TW |
| dc.subject | vicariance effect | en |
| dc.subject | biogeography | en |
| dc.subject | species delimitation | en |
| dc.subject | morphology | en |
| dc.subject | phylogeny | en |
| dc.subject | vicariance effect | en |
| dc.subject | cryptic species | en |
| dc.subject | genus Lepas | en |
| dc.subject | goose barnacle | en |
| dc.subject | biogeography | en |
| dc.subject | species delimitation | en |
| dc.subject | morphology | en |
| dc.subject | goose barnacle | en |
| dc.subject | genus Lepas | en |
| dc.subject | cryptic species | en |
| dc.subject | phylogeny | en |
| dc.title | 台灣海域茗荷藤壺之隱蔽種多樣性 | zh_TW |
| dc.title | Cryptic diversity of goose-neck barnacle Lepas in Taiwan waters | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 施習德;町田龍二 | zh_TW |
| dc.contributor.oralexamcommittee | Hsi-Te Shih;Ryuji Machida | en |
| dc.subject.keyword | 鵝頸藤壺,茗荷屬,隱蔽種,地理隔離,親緣關係,形態,物種界定,生物地理學, | zh_TW |
| dc.subject.keyword | goose barnacle,genus Lepas,cryptic species,vicariance effect,phylogeny,morphology,species delimitation,biogeography, | en |
| dc.relation.page | 203 | - |
| dc.identifier.doi | 10.6342/NTU202304335 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2023-10-18 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 生態學與演化生物學研究所 | - |
| dc.date.embargo-lift | 2028-10-16 | - |
| Appears in Collections: | 生態學與演化生物學研究所 | |
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| ntu-112-1.pdf Restricted Access | 16.89 MB | Adobe PDF | View/Open |
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