請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91199完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 靳宗洛 | zh_TW |
| dc.contributor.advisor | Tsung-Luo Jinn | en |
| dc.contributor.author | 許宇豪 | zh_TW |
| dc.contributor.author | Yu-Hao Xu | en |
| dc.date.accessioned | 2023-12-12T16:10:31Z | - |
| dc.date.available | 2023-12-13 | - |
| dc.date.copyright | 2023-12-12 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-11-23 | - |
| dc.identifier.citation | Acosta-Motos, J.R., Ortuño, M.F., Bernal-Vicente, A., Diaz-Vivancos, P., Sanchez-Blanco, M.J., and Hernandez, J.A. (2017). Plant Responses to Salt Stress: Adaptive Mechanisms. Agronomy 7, 18.
Allakhverdiev, S.I., Kreslavski, V.D., Klimov, V.V., Los, D.A., Carpentier, R., and Mohanty, P. (2008). Heat stress: an overview of molecular responses in photosynthesis. Photosynthesis research 98, 541-550. Anders, S., McCarthy, D.J., Chen, Y., Okoniewski, M., Smyth, G.K., Huber, W., and Robinson, M.D. (2013). Count-based differential expression analysis of RNA sequencing data using R and Bioconductor. Nat Protoc 8, 1765-1786. Andrási, N., Pettkó-Szandtner, A., and Szabados, L. (2020). Diversity of plant heat shock factors: regulation, interactions, and functions. Journal of Experimental Botany 72, 1558-1575. Apse, M.P., Aharon, G.S., Snedden, W.A., and Blumwald, E. (1999). Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis. Science 285, 1256-1258. Baniwal, S.K., Chan, K.Y., Scharf, K.-D., and Nover, L. (2007). Role of heat stress transcription factor HsfA5 as specific repressor of HsfA4. Journal of Biological Chemistry 282, 3605-3613. Begum, T., Reuter, R., and Schöffl, F. (2013). Overexpression of AtHsfB4 induces specific effects on root development of Arabidopsis. Mech Dev 130, 54-60. Bhuiyan, N.H., Rowland, E., Friso, G., Ponnala, L., Michel, E.J.S., and van Wijk, K.J. (2020). Autocatalytic Processing and Substrate Specificity of Arabidopsis Chloroplast Glutamyl Peptidase. Plant Physiol 184, 110-129. Blum, A. (2005). Drought resistance, water-use efficiency, and yield potential—are they compatible, dissonant, or mutually exclusive? Australian Journal of Agricultural Research 56, 1159-1168. Bolger, A.M., Lohse, M., and Usadel, B. (2014). Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30, 2114-2120. Bonetta, D., and McCourt, P. (1998). Genetic analysis of ABA signal transduction pathways. Trends in Plant Science 3, 231-235. Bray, E.A. (1997). Plant responses to water deficit. Trends in Plant Science 2, 48-54. Charng, Y.Y., Liu, H.C., Liu, N.Y., Hsu, F.C., and Ko, S.S. (2006). Arabidopsis Hsa32, a novel heat shock protein, is essential for acquired thermotolerance during long recovery after acclimation. Plant Physiol 140, 1297-1305. Charng, Y.Y., Liu, H.C., Liu, N.Y., Chi, W.T., Wang, C.N., Chang, S.H., and Wang, T.T. (2007). A heat-inducible transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis. Plant Physiol 143, 251-262. Cheeseman, J.M. (1988). Mechanisms of Salinity Tolerance in Plants Plant Physiology 87, 547-550. Chow, C.N., Zheng, H.Q., Wu, N.Y., Chien, C.H., Huang, H.D., Lee, T.Y., Chiang-Hsieh, Y.F., Hou, P.F., Yang, T.Y., and Chang, W.C. (2016). PlantPAN 2.0: an update of plant promoter analysis navigator for reconstructing transcriptional regulatory networks in plants. Nucleic Acids Res 44, D1154-1160. Clos, J., Westwood, J.T., Becker, P.B., Wilson, S., Lambert, K., and Wu, C. (1990). Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation. Cell 63, 1085-1097. Clough, S.J., and Bent, A.F. (1998). Floral dip: a simplified method for Agrobacterium‐mediated transformation of Arabidopsis thaliana. The plant journal 16, 735-743. Cortijo, S., Charoensawan, V., Brestovitsky, A., Buning, R., Ravarani, C., Rhodes, D., van Noort, J., Jaeger, K.E., and Wigge, P.A. (2017). Transcriptional regulation of the ambient temperature response by H2A. Z nucleosomes and HSF1 transcription factors in Arabidopsis. Molecular plant 10, 1258-1273. Czechowski, T., Stitt, M., Altmann, T., Udvardi, M.K., and Scheible, W.R. (2005). Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol 139, 5-17. Deinlein, U., Stephan, A.B., Horie, T., Luo, W., Xu, G., and Schroeder, J.I. (2014). Plant salt-tolerance mechanisms. Trends in Plant Science 19, 371-379. Demidchik, V. (2015). Mechanisms of oxidative stress in plants: from classical chemistry to cell biology. Environmental and experimental botany 109, 212-228. Ding, Y., Fromm, M., and Avramova, Z. (2012). Multiple exposures to drought 'train' transcriptional responses in Arabidopsis. Nat Commun 3, 740. Ding, Y., Shi, Y., and Yang, S. (2020). Molecular Regulation of Plant Responses to Environmental Temperatures. Mol Plant 13, 544-564. Eamus, D., Duff, G.A., and Berryman, C.A. (1995). Photosynthetic responses to temperature, light flux-density, CO2 concentration and vapour pressure deficit in Eucalyptus tetrodonta grown under CO2 enrichment. Environmental Pollution 90, 41-49. Edwards, K., Johnstone, C., and Thompson, C. (1991). A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research 19, 1349-1349. Engler, C., Kandzia, R., and Marillonnet, S. (2008). A One Pot, One Step, Precision Cloning Method with High Throughput Capability. PLOS ONE 3, e3647. Ewels, P., Magnusson, M., Lundin, S., and Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics 32, 3047-3048. Fahad, S., Bajwa, A.A., Nazir, U., Anjum, S.A., Farooq, A., Zohaib, A., Sadia, S., Nasim, W., Adkins, S., Saud, S., Ihsan, M.Z., Alharby, H., Wu, C., Wang, D., and Huang, J. (2017). Crop Production under Drought and Heat Stress: Plant Responses and Management Options. Front Plant Sci 8, 1147. Fang, Y., and Xiong, L. (2015). General mechanisms of drought response and their application in drought resistance improvement in plants. Cellular and molecular life sciences 72, 673-689. Franklin, K.A., Lee, S.H., Patel, D., Kumar, S.V., Spartz, A.K., Gu, C., Ye, S., Yu, P., Breen, G., Cohen, J.D., Wigge, P.A., and Gray, W.M. (2011). Phytochrome-interacting factor 4 (PIF4) regulates auxin biosynthesis at high temperature. Proc Natl Acad Sci U S A 108, 20231-20235. Friedrich, T., Oberkofler, V., Trindade, I., Altmann, S., Brzezinka, K., Lamke, J., Gorka, M., Kappel, C., Sokolowska, E., Skirycz, A., Graf, A., and Baurle, I. (2021). Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis. Nat Commun 12, 3426. Giesguth, M., Sahm, A., Simon, S., and Dietz, K.-J. (2015). Redox-dependent translocation of the heat shock transcription factor AtHSFA8 from the cytosol to the nucleus in Arabidopsis thaliana. FEBS letters 589, 718-725. Goldenberg, E.P. (1988). Mathematics, metaphors, and human factors: Mathematical, technical, and pedagogical challenges in the educational use of graphical representation of functions. The Journal of Mathematical Behavior. Golldack, D., Li, C., Mohan, H., and Probst, N. (2014). Tolerance to drought and salt stress in plants: Unraveling the signaling networks. Frontiers in Plant Science 5. Gomez-Pastor, R., Burchfiel, E.T., and Thiele, D.J. (2018). Regulation of heat shock transcription factors and their roles in physiology and disease. Nature Reviews Molecular Cell Biology 19, 4-19. Guo, M., Liu, J.-H., Ma, X., Luo, D.-X., Gong, Z.-H., and Lu, M.-H. (2016). The Plant Heat Stress Transcription Factors (HSFs): Structure, Regulation, and Function in Response to Abiotic Stresses. Frontiers in Plant Science 7. Hasanuzzaman, M., Nahar, K., Alam, M.M., Roychowdhury, R., and Fujita, M. (2013). Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. Int J Mol Sci 14, 9643-9684. Hayes, S., Schachtschabel, J., Mishkind, M., Munnik, T., and Arisz, S.A. (2021). Hot topic: Thermosensing in plants. Plant Cell Environ 44, 2018-2033. Howell, S.H. (2021). Evolution of the unfolded protein response in plants. Plant Cell Environ 44, 2625-2635. Hu, S., Ding, Y., and Zhu, C. (2020). Sensitivity and Responses of Chloroplasts to Heat Stress in Plants. Frontiers in Plant Science 11. Huang, G.-T., Ma, S.-L., Bai, L.-P., Zhang, L., Ma, H., Jia, P., Liu, J., Zhong, M., and Guo, Z.-F. (2012). Signal transduction during cold, salt, and drought stresses in plants. Molecular Biology Reports 39, 969-987. Huang, Y.C., Niu, C.Y., Yang, C.R., and Jinn, T.L. (2016). The Heat Stress Factor HSFA6b Connects ABA Signaling and ABA-Mediated Heat Responses. Plant Physiol 172, 1182-1199. Hwang, S.M., Kim, D.W., Woo, M.S., Jeong, H.S., Son, Y.S., Akhter, S., Choi, G.J., and Bahk, J.D. (2014). Functional characterization of A rabidopsis HsfA6a as a heat‐shock transcription factor under high salinity and dehydration conditions. Plant, cell & environment 37, 1202-1222. Ikeda, M., Mitsuda, N., and Ohme-Takagi, M. (2011). Arabidopsis HsfB1 and HsfB2b Act as Repressors of the Expression of Heat-Inducible Hsfs But Positively Regulate the Acquired Thermotolerance Plant Physiology 157, 1243-1254. Kanehisa, M., Sato, Y., Furumichi, M., Morishima, K., and Tanabe, M. (2019). New approach for understanding genome variations in KEGG. Nucleic Acids Res 47, D590-d595. Kanehisa, M., Araki, M., Goto, S., Hattori, M., Hirakawa, M., Itoh, M., Katayama, T., Kawashima, S., Okuda, S., Tokimatsu, T., and Yamanishi, Y. (2008). KEGG for linking genomes to life and the environment. Nucleic Acids Res 36, D480-484. Kim, D., Langmead, B., and Salzberg, S.L. (2015). HISAT: a fast spliced aligner with low memory requirements. Nature Methods 12, 357-360. Koini, M.A., Alvey, L., Allen, T., Tilley, C.A., Harberd, N.P., Whitelam, G.C., and Franklin, K.A. (2009). High temperature-mediated adaptations in plant architecture require the bHLH transcription factor PIF4. Curr Biol 19, 408-413. Kotak, S., Vierling, E., Bäumlein, H., and Koskull-Döring, P.v. (2007). A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development of Arabidopsis. The Plant Cell 19, 182-195. Kumar, S.V., and Wigge, P.A. (2010). H2A. Z-containing nucleosomes mediate the thermosensory response in Arabidopsis. Cell 140, 136-147. Lamke, J., Brzezinka, K., Altmann, S., and Baurle, I. (2016). A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory. EMBO J 35, 162-175. Larkindale, J., Hall, J.D., Knight, M.R., and Vierling, E. (2005). Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant physiology 138, 882-897. Li, L., Foster, C.M., Gan, Q., Nettleton, D., James, M.G., Myers, A.M., and Wurtele, E.S. (2009). Identification of the novel protein QQS as a component of the starch metabolic network in Arabidopsis leaves. Plant J 58, 485-498. Liao, Y., Smyth, G.K., and Shi, W. (2014). featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics 30, 923-930. Lin, K.F., Tsai, M.Y., Lu, C.A., Wu, S.J., and Yeh, C.H. (2018). The roles of Arabidopsis HSFA2, HSFA4a, and HSFA7a in the heat shock response and cytosolic protein response. Bot Stud 59, 15. Liu, H., Able, A.J., and Able, J.A. (2022). Priming crops for the future: rewiring stress memory. Trends in Plant Science 27, 699-716. Liu, H.C., and Charng, Y.Y. (2013). Common and distinct functions of Arabidopsis class A1 and A2 heat shock factors in diverse abiotic stress responses and development. Plant Physiol 163, 276-290. Liu, H.C., Liao, H.T., and Charng, Y.Y. (2011). The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis. Plant Cell Environ 34, 738-751. Liu, H.C., Lämke, J., Lin, S.Y., Hung, M.J., Liu, K.M., Charng, Y.Y., and Bäurle, I. (2018). Distinct heat shock factors and chromatin modifications mediate the organ-autonomous transcriptional memory of heat stress. Plant J 95, 401-413. Love, M.I., Huber, W., and Anders, S. (2014). Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology 15, 550. Ma, X., Zhang, Q., Zhu, Q., Liu, W., Chen, Y., Qiu, R., Wang, B., Yang, Z., Li, H., Lin, Y., Xie, Y., Shen, R., Chen, S., Wang, Z., Chen, Y., Guo, J., Chen, L., Zhao, X., Dong, Z., and Liu, Y.-G. (2015). A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. Molecular Plant 8, 1274-1284. Manghwar, H., and Li, J. (2022). Endoplasmic Reticulum Stress and Unfolded Protein Response Signaling in Plants. Int J Mol Sci 23. Mesihovic, A., Ullrich, S., Rosenkranz, R.R.E., Gebhardt, P., Bublak, D., Eich, H., Weber, D., Berberich, T., Scharf, K.-D., Schleiff, E., and Fragkostefanakis, S. (2022). HsfA7 coordinates the transition from mild to strong heat stress response by controlling the activity of the master regulator HsfA1a in tomato. Cell Reports 38, 110224. Misra, S., Wu, Y., Venkataraman, G., Sopory, S.K., and Tuteja, N. (2007). Heterotrimeric G-protein complex and G-protein-coupled receptor from a legume (Pisum sativum): role in salinity and heat stress and cross-talk with phospholipase C. The Plant Journal 51, 656-669. Mittler, R., Finka, A., and Goloubinoff, P. (2012). How do plants feel the heat? Trends in Biochemical Sciences 37, 118-125. Msanne, J., Lin, J., Stone, J.M., and Awada, T. (2011). Characterization of abiotic stress-responsive Arabidopsis thaliana RD29A and RD29B genes and evaluation of transgenes. Planta 234, 97-107. Munemasa, S., Hauser, F., Park, J., Waadt, R., Brandt, B., and Schroeder, J.I. (2015). Mechanisms of abscisic acid-mediated control of stomatal aperture. Current Opinion in Plant Biology 28, 154-162. Narusaka, Y., Nakashima, K., Shinwari, Z.K., Sakuma, Y., Furihata, T., Abe, H., Narusaka, M., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2003). Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses. Plant J 34, 137-148. Nawkar, G.M., Lee, E.S., Shelake, R.M., Park, J.H., Ryu, S.W., Kang, C.H., and Lee, S.Y. (2018). Activation of the Transducers of Unfolded Protein Response in Plants. Front Plant Sci 9, 214. Nover, L., Bharti, K., Döring, P., Mishra, S.K., Ganguli, A., and Scharf, K.-D. (2001). Arabidopsis and the Heat Stress Transcription Factor World: How Many Heat Stress Transcription Factors Do We Need? Cell stress & chaperones 6, 177. Ohama, N., Sato, H., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2017). Transcriptional Regulatory Network of Plant Heat Stress Response. Trends Plant Sci 22, 53-65. Ohama, N., Kusakabe, K., Mizoi, J., Zhao, H., Kidokoro, S., Koizumi, S., Takahashi, F., Ishida, T., Yanagisawa, S., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2015). The Transcriptional Cascade in the Heat Stress Response of Arabidopsis Is Strictly Regulated at the Level of Transcription Factor Expression. The Plant Cell 28, 181-201. Pick, T., Jaskiewicz, M., Peterhänsel, C., and Conrath, U. (2012). Heat Shock Factor HsfB1 Primes Gene Transcription and Systemic Acquired Resistance in Arabidopsis Plant Physiology 159, 52-55. Quint, M., Delker, C., Franklin, K.A., Wigge, P.A., Halliday, K.J., and van Zanten, M. (2016). Molecular and genetic control of plant thermomorphogenesis. Nat Plants 2, 15190. Sakuma, Y., Maruyama, K., Qin, F., Osakabe, Y., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2006). Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proc Natl Acad Sci U S A 103, 18822-18827. Salvucci, M.E., and Crafts-Brandner, S.J. (2004). Inhibition of photosynthesis by heat stress: the activation state of Rubisco as a limiting factor in photosynthesis. Physiol Plant 120, 179-186. Savchenko, G., Klyuchareva, E., Abramchik, L., and Serdyuchenko, E. (2002). Effect of periodic heat shock on the inner membrane system of etioplasts. Russian journal of plant physiology 49, 349-359. Schramm, F., Ganguli, A., Kiehlmann, E., Englich, G., Walch, D., and von Koskull-Doring, P. (2006). The heat stress transcription factor HsfA2 serves as a regulatory amplifier of a subset of genes in the heat stress response in Arabidopsis. Plant Mol Biol 60, 759-772. Singer, T., Fan, Y., Chang, H.S., Zhu, T., Hazen, S.P., and Briggs, S.P. (2006). A high-resolution map of Arabidopsis recombinant inbred lines by whole-genome exon array hybridization. PLoS Genet 2, e144. Soon, F.-F., Ng, L.-M., Zhou, X.E., West, G.M., Kovach, A., Tan, M.H.E., Suino-Powell, K.M., He, Y., Xu, Y., Chalmers, M.J., Brunzelle, J.S., Zhang, H., Yang, H., Jiang, H., Li, J., Yong, E.-L., Cutler, S., Zhu, J.-K., Griffin, P.R., Melcher, K., and Xu, H.E. (2012). Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases. Science 335, 85-88. Sorger, P.K., and Pelham, H.R. (1988). Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation. Cell 54, 855-864. Stavang, J.A., Gallego-Bartolomé, J., Gómez, M.D., Yoshida, S., Asami, T., Olsen, J.E., García-Martínez, J.L., Alabadí, D., and Blázquez, M.A. (2009). Hormonal regulation of temperature-induced growth in Arabidopsis. Plant J 60, 589-601. Tang, R.H., Han, S., Zheng, H., Cook, C.W., Choi, C.S., Woerner, T.E., Jackson, R.B., and Pei, Z.M. (2007). Coupling diurnal cytosolic Ca2+ oscillations to the CAS-IP3 pathway in Arabidopsis. Science 315, 1423-1426. Tasset, C., Singh Yadav, A., Sureshkumar, S., Singh, R., van der Woude, L., Nekrasov, M., Tremethick, D., van Zanten, M., and Balasubramanian, S. (2018). POWERDRESS-mediated histone deacetylation is essential for thermomorphogenesis in Arabidopsis thaliana. PLoS Genetics 14, e1007280. TEJEDOR‐CANO, J., PRIETO‐DAPENA, P., Almoguera, C., Carranco, R., Hiratsu, K., OHME‐TAKAGI, M., and Jordano, J. (2010). Loss of function of the HSFA9 seed longevity program. Plant, Cell & Environment 33, 1408-1417. Tubiello, F.N., Soussana, J.-F., and Howden, S.M. (2007). Crop and pasture response to climate change. Proceedings of the National Academy of Sciences 104, 19686-19690. Vierling, E. (1991). The roles of heat shock proteins in plants PAnnual Review of Plant Physiology and Plant Molecular Biology 42, 579-620. von Koskull-Döring, P., Scharf, K.D., and Nover, L. (2007). The diversity of plant heat stress transcription factors. Trends Plant Sci 12, 452-457. Vu, L.D., Gevaert, K., and De Smet, I. (2019). Feeling the Heat: Searching for Plant Thermosensors. Trends Plant Sci 24, 210-219. Wahid, A., Gelani, S., Ashraf, M., and Foolad, M.R. (2007). Heat tolerance in plants: an overview. Environmental and experimental botany 61, 199-223. Wang, L., Feng, Z., Wang, X., Wang, X., and Zhang, X. (2010). DEGseq: an R package for identifying differentially expressed genes from RNA-seq data. Bioinformatics 26, 136-138. Wang, Z.P., Xing, H.L., Dong, L., Zhang, H.Y., Han, C.Y., Wang, X.C., and Chen, Q.J. (2015). Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol 16, 144. Wenjing, W., Chen, Q., Singh, P.K., Huang, Y., and Pei, D. (2020). CRISPR/Cas9 edited HSFA6a and HSFA6b of Arabidopsis thaliana offers ABA and osmotic stress insensitivity by modulation of ROS homeostasis. Plant Signaling & Behavior 15, 1816321. Wu, C. (1995). Heat Shock Transcription Factors: Structure and Regulation. Annual Review of Cell and Developmental Biology 11, 441-469. Wunderlich, M., Groß-Hardt, R., and Schöffl, F. (2014). Heat shock factor HSFB2a involved in gametophyte development of Arabidopsis thaliana and its expression is controlled by a heat-inducible long non-coding antisense RNA. Plant molecular biology 85, 541-550. Yeh, C.H., Kaplinsky, N.J., Hu, C., and Charng, Y.Y. (2012). Some like it hot, some like it warm: phenotyping to explore thermotolerance diversity. Plant Sci 195, 10-23. Yoshida, T., Mogami, J., and Yamaguchi-Shinozaki, K. (2014). ABA-dependent and ABA-independent signaling in response to osmotic stress in plants. Current Opinion in Plant Biology 21, 133-139. Yoshida, T., Sakuma, Y., Todaka, D., Maruyama, K., Qin, F., Mizoi, J., Kidokoro, S., Fujita, Y., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2008). Functional analysis of an Arabidopsis heat-shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stress-regulatory system. Biochem Biophys Res Commun 368, 515-521. Yu, G., Wang, L.G., Han, Y., and He, Q.Y. (2012). clusterProfiler: an R package for comparing biological themes among gene clusters. Omics 16, 284-287. Yue, B., Xue, W., Xiong, L., Yu, X., Luo, L., Cui, K., Jin, D., Xing, Y., and Zhang, Q. (2006). Genetic basis of drought resistance at reproductive stage in rice: separation of drought tolerance from drought avoidance. Genetics 172, 1213-1228. Zang, D., Wang, J., Zhang, X., Liu, Z., and Wang, Y. (2019). Arabidopsis heat shock transcription factor HSFA7b positively mediates salt stress tolerance by binding to an E-box-like motif to regulate gene expression. J Exp Bot 70, 5355-5374. Zhu, J.-K. (2001). Plant salt tolerance. Trends in Plant Science 6, 66-71. Zhu, J.-K. (2002). Salt and drought stress signal transduction in plants. Annual review of plant biology 53, 247-273. Zhu, J.-K., Liu, J., and Xiong, L. (1998). Genetic analysis of salt tolerance in Arabidopsis: evidence for a critical role of potassium nutrition. The Plant Cell 10, 1181-1191. 張凌誌. (2021). 阿拉伯芥熱休克因子 HSFC1 在溫度逆境下的表達調控. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91199 | - |
| dc.description.abstract | 植物熱休克轉錄因子（HSFs）調節包括高溫、乾旱、低溫、鹽度和氧化壓力逆境在內等非生物逆境反應。HSFA7a 和 HSFA7b 在演化上具有親緣關係，暗示著這兩個基因可能共同參與調控非生物逆境反應。利用CRISPR-Cas9技術建立HSFA7a 和 HSFA7b的雙突變株，其在75 mM氯化鈉或100 mM甘露醇琼脂培養基上生長的根長度較野生型短。此外，HSFA7b缺失株在滲透壓逆境下呈現不敏感的表現型。關於下胚軸延長的實驗結果顯示，雙突變植物對於先天耐熱性（BT）不敏感，但在後天短期耐熱性（SAT）條件下呈現敏感的表型。耐熱性分析的結果顯示，在SAT和梯度熱逆境（GHS）下，HSFA7a和HSFA7b共同負調控植物耐熱性；然而HSFA7b單獨正向調控長期後天耐熱性(LAT)。有鑒於這些表型，植株在GHS和LAT熱處理後進行了RNA-seq轉錄體分析，以尋找由HSFA7a和HSFA7b所調控的下游基因。我們觀察到在兩個數據集中關於光合作用相關的上調差異表達基因（DEGs）重疊，暗示這兩個基因可能參與光合作用過程。總而言之，我們的結果突顯了HSFA7a和HSFA7b在不同非生物逆境下的複雜調控機制，並識別出潛在的下游基因，將進一步進行分析。 | zh_TW |
| dc.description.abstract | Plant heat shock factors (HSFs) are crucial in orchestrating responses to various abiotic stresses, including high and low temperatures, drought, salinity, and oxidative stress. HSFA7a and HSFA7b exhibit a shared evolutionary lineage, suggesting their possible collaboration in the regulation of abiotic stress responses. Leveraging CRISPR-Cas9 technology, we generated double mutant lines of HSFA7a and HSFA7b, which exhibited reduced root lengths when exposed to 75 mM sodium chloride or 100 mM mannitol treatments compared to wild-type plants. Furthermore, HSFA7b-deficient mutants displayed insensitivity to osmotic stress. Our experimental findings on hypocotyl elongation indicated that these double mutant plants were less responsive to basal thermotolerance (BT) yet displayed heightened sensitivity under short-term acquired thermotolerance (SAT) conditions. Heat tolerance analyses unveiled that HSFA7a and HSFA7b jointly exerted a negative regulatory influence on plant thermotolerance during SAT and gradient heat stress (GHS) scenarios, with HSFA7b demonstrating independent positive regulation of long-term acquired thermotolerance (LAT). Given these distinctive phenotypes, we conducted RNA-seq transcriptome analyses on plants subjected to GHS and LAT heat treatments to identify downstream genes under the regulatory influence of HSFA7a and HSFA7b. Remarkably, we observed a convergence of upregulated differentially expressed genes (DEGs) associated with photosynthesis in both datasets, suggesting the potential involvement of these genes in the photosynthesis process. In summary, our findings shed light on the complex regulatory mechanisms governing HSFA7a and HSFA7b responses to diverse abiotic stresses and identify candidate downstream genes warranting further investigation. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-12-12T16:10:31Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-12-12T16:10:31Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 致謝 i
摘要 ii Abstract iii List of figures viii List of tables x Abbreviations xi Introduction 1 Heat Stress/ Shock (HS) 1 Heat Shock Factors (HSFs) 2 Heat Stress Response 7 Salt Stress 11 Drought Stress 13 Objectives 19 Material and Methods 20 Plant materials and growth conditions 20 Generation of transgenic HSFA7a and HSFA7b-overexpression lines 22 DNA/RNA preparation, cDNA synthesis and qRT-PCR 23 Thermotolerance tests 25 Protein extraction and immunoblotting assay for 35S::HSFA7b lines 26 Ponceau S staining 27 Root elongation tests under acquired thermotolerance conditions 27 Root growth tests under salt and osmotic stresses either individually or in combination with heat stress. 28 Thermomorphogenesis analysis for different lines 29 Hypocotyl elongation 29 RNA-seq analysis 30 Results 32 Transcription profiling of HSFA7a and HSFA7b in response to heat shock (HS) 32 Identification and characterization of the single mutants and double mutants of HSFA7a and HSFA7b 32 Phenotyping of mutants by quantitation of the root elongation in response to different abiotic stresses 35 Phenotyping of mutants by quantitation of the hypocotyl elongation in response to heat stress (HS) 38 Thermomorphogenesis analysis for mutant lines under high temperatures 39 Basal thermotolerance and short-term acquired thermotolerance analysis for mutants 40 Long-term acquired thermotolerance and gradient heat stress analysis for mutants 41 RNA-seq transcriptome analysis under LAT test in hsfa7acas9-1 42 RNA-seq transcriptome analysis under GHS test in hsfa7acas9-1 44 The expression level of HSFA7a and HSFA7b under SAT and LAT tests 46 HSFA7a and HSFA7b did not affect each other's expression levels. 47 HSFA7a and HSFA7b were not heat-memory genes 47 Discussion 50 HSFA7a and HSFA7b were HS-induced genes that exhibited expression under normal conditions. 50 Generation of double mutant plants was difficult by crossing strategy 52 HSFA7a and HSFA7b might participate in root development. 54 The root elongation phenotypical analysis for mutants in response to abiotic stress. 55 HSFA7a and HSFA7b might play a role in thermomorphogenesis process. 58 The complex mechanisms of HSFA7a and HSFA7b regulated plant’s thermotolerance. 60 RNA-seq analysis in LAT and GHS test 63 Tables 67 Figures 80 Supplemental Figures 104 References 114 Appendix 124 | - |
| dc.language.iso | en | - |
| dc.subject | 非生物逆境 | zh_TW |
| dc.subject | 阿拉伯芥 | zh_TW |
| dc.subject | 熱休克轉錄因子 | zh_TW |
| dc.subject | 非生物逆境 | zh_TW |
| dc.subject | 核糖核酸定序 | zh_TW |
| dc.subject | 阿拉伯芥 | zh_TW |
| dc.subject | 熱休克轉錄因子 | zh_TW |
| dc.subject | 耐熱性 | zh_TW |
| dc.subject | 核糖核酸定序 | zh_TW |
| dc.subject | 耐熱性 | zh_TW |
| dc.subject | abiotic stress | en |
| dc.subject | RNA-seq | en |
| dc.subject | thermotolerance | en |
| dc.subject | abiotic stress | en |
| dc.subject | Arabidopsis | en |
| dc.subject | Heat shock factor | en |
| dc.subject | RNA-seq | en |
| dc.subject | Heat shock factor | en |
| dc.subject | Arabidopsis | en |
| dc.subject | thermotolerance | en |
| dc.title | 阿拉伯芥熱休克轉錄因子HsfA7a和HsfA7b參與非生物逆境反應之功能性研究 | zh_TW |
| dc.title | Functional study of heat shock transcription factors HsfA7a and HsfA7b required for abiotic stress responses in Arabidopsis | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 李金美;吳少傑;常怡雍 | zh_TW |
| dc.contributor.oralexamcommittee | Chin-Mei Lee;Shaw-Jye Wu;Yee-Yung Charng | en |
| dc.subject.keyword | 熱休克轉錄因子,阿拉伯芥,非生物逆境,耐熱性,核糖核酸定序, | zh_TW |
| dc.subject.keyword | Heat shock factor,Arabidopsis,abiotic stress,thermotolerance,RNA-seq, | en |
| dc.relation.page | 127 | - |
| dc.identifier.doi | 10.6342/NTU202304437 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2023-11-24 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 植物科學研究所 | - |
| 顯示於系所單位： | 植物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-112-1.pdf | 13.62 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。