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  1. NTU Theses and Dissertations Repository
  2. 生物資源暨農學院
  3. 園藝暨景觀學系
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9107
完整後設資料紀錄
DC 欄位值語言
dc.contributor.advisor李國譚(Kuo-Tan Li)
dc.contributor.authorLan-Yen Changen
dc.contributor.author張嵐雁zh_TW
dc.date.accessioned2021-05-20T20:09:28Z-
dc.date.available2009-07-31
dc.date.available2021-05-20T20:09:28Z-
dc.date.copyright2009-07-31
dc.date.issued2009
dc.date.submitted2009-07-30
dc.identifier.citation第一章 參考文獻
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Zhao, W., Y. Pan, Z. Zhang, S. Jia, X. Miao, and Y. Huang. 2005. Phylogeny of the genus Morus (Urticales: Moraceae) inferred from ITS and trnL-F sequences. Afr. J. Biotech. 4:563-569.
Zhao, W., Y. Wang, T. Chen, G. Jia, X. Wang, J. Qi, Y. Pan, S. Wang, Z. Li, Y. Huang, Y. Pan, and Y.-H. Yang. 2007. Genetic structure of mulberry from different ecotypes revealed by ISSRs in China: An implications for conservation of local mulberry varieties. Sci. Hort. 115:47-55.
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第四章 參考文獻
張哲嘉. 2006. 臺灣桑樹之分類及品種改良. 臺灣園藝 52:377-392.
儲瑞銀、孫曉霞. 1986. 桑屬植物細胞遺傳學的研究 I. 部分桑品種的染色體數. 12: 199-202.
儲瑞銀、孫曉霞. 1987. 桑屬植物細胞遺傳學的研究 II. 四個桑品種的核型分析. 13: 129-132.
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大澤一衛. 1916. 桑の細胞學并に實驗的研究. 蚕試報告 1:215-292.
南澤吉三郎. 1976. 栽桑學. p.108-114. 鳳鳴社. 東京. 日本.
關 博夫. 1956. 桑樹の性染色体について. 日蚕雑. 25:191. (予報)
關 博夫. 1959. 桑属植物の細胞学的研究. 信州大繊紀要. 20:1-91.
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Zhao, W., Y. Pan, Z. Zhang, S. Jia, X. Miao, and Y. Huang. 2005. Phlogeny of the genus Morus (Urticales: Moraceae) inferred from ITS and trnL-F sequences. Afr. J. Biotechnol. 4:563-569.
Zhao, W., Y. Wang, T. Chen, G. Jia, X. Wang, J. Qi, Y. Pang, S. Wang, Z. Li, Y. Huang, Y. Pan, and Y.H. Yang. 2007. Genetic structure of mulberry from different ecotypes revealed by ISSRs in China: An implications for conservation of local mulberry varieties. Sci. Hort. 115: 47–55.
第五章 參考文獻
吳登楨. 1977. 桑樹之品種改良. 科學農業 25:242-244.
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張哲嘉. 2006. 臺灣桑樹之分類及品種改良. 臺灣園藝 52:377-392.
曾富生、吳登楨. 1979. 臺灣野生桑樹(Morus acidosa Griff.)農藝性狀之變異. 興大農藝學報 4:39-45.
Dandin, S. B. 1998. Mulberry: a versatile biosource in the service of mankind. Acta Sericologica Sinica 24:109-113.
Dobigny, G, J-F Ducroz, T. J. Robinson, and V. Volobouev. 2004. Cytogenetics and cladistics. Syst. Biol. 53:470-484.
Ercisli, S. and E. Orhan. 2007. Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits. Food Chem. 103: 1380-1384.
Guerra, M. 2008. Chromosome numbers in plant cytotaxonomy: concepts and implications. Cytogenet. Genome Res. 120:339-350.
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Venkataraman, K. 1972. Wood phenolics in the chemotaxonomy of the Moraceae. Phytochemistry 11: 1571-1586.
Zhao, W., Y. Wang, T. Chen, G. Jia, X. Wang, J. Qi, Y. Pang, S. Wang, Z. Li, Y. Huang, Y. Pan, and Y.H. Yang. 2007. Genetic structure of mulberry from different ecotypes revealed by ISSRs in China: An implications for conservation of local mulberry varieties. Sci. Hort. 115:47-55.
dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9107-
dc.description.abstract摘要
  桑樹(Morus spp.)已引入臺灣多年,為具發展潛力之多年生作物,多為雌雄異株,行異花授粉且分布廣泛,遺傳背景複雜,其分類尚待釐清。為瞭解臺灣桑樹種原間之親緣關係,本研究利用營養性狀進行歧異度分析,並以流式細胞儀及核型分析確認其倍體數,俾建立臺灣桑樹細胞遺傳資料庫。
  自苗栗區農業改良場桑樹種原庫選出26個品種(系),在2008年調查記錄各品種(系)之21組營養性狀。經主成份分析及群集分析,受試品種(系)可分為三群(一)長果桑(Morus laevigata),(二)廣東桑(M. atropurpurea)、山桑(M. bombycis)、島桑(M. australis)及臺灣桑(M. formosensis),及(三)白桑(M. alba)及魯桑(M. latifolia),群內親緣相近。從中選出葉寬、葉長葉寬比、葉柄寬、休眠需冷量、生長中芽形、休眠芽形、葉色、葉緣及葉尖等九個主要代表性狀,歸納出不受限於株性之桑樹檢索表,可供分類使用。
  為估算種原倍體數,以流式細胞儀進行分析,結果顯示參試品種(系)可分為兩類,二倍體包括山桑、白桑、魯桑、島桑、臺灣桑及廣東桑,DNA含量為0.61-0.71 pg/2C。70C006 (M. laevigata) 為三倍體,DNA含量為1.06 pg/2C。67C001 (M. australis) 則有基因組各為0.63 pg/2C的二倍體和0.98 pg/2C的三倍體株,倍體數之差異亦會表現在形態上,三倍體植株較二倍體植株大。
  桑樹13個品種(系)之染色體核型一致,14對染色體中,僅第2、5及14對為次中位中節,其餘為中位中節,以螢光原位雜交技術(Fluorescence in situ hybridization, FISH)標定白桑、島桑、廣東桑及長果桑之rDNA基因座位置,分佈位置一致,種間無明顯差異。不同株性的品種(系)中,唯島桑及雜交桑之雄株品種(系),在第3對染色體有一條染色體之短臂染色深,但白桑不同株性品種(系)間則無條帶差異。
zh_TW
dc.description.abstractAbstract
The mulberry (Morus spp.), a perennial crop valued for its economoical importance, had led it a potential crop in the future. Because of its dioecious and cross-pollination nature, a complex genetic background of mulberry cultivars has been developed in Taiwan and the original classification system may need to be reexamined. In this study, the genetic relationship of the mulberry collection in Taiwan has been established by principal component analysis and clustering analysis based on vegetative traits. Besides, the ploidy and karyotype have also been examined to enrich the cytogenetic data base.
Twenty-six mulberry accessions from Miaoli District Agricultural Research and Extension Station (MDARES) were selected and 21 morphological characters were evaluated in 2008. Data were used for principal component analysis and clustering analysis. Accessions were grouped into three major clusters: (1) Morus laevigata, (2) M. atropurpurea, M. bombycis, M. australis, M. formosensis, and (3) M. alba and M. latifolia. Nine sets of morphological characters including leaf width, leaf lengths : widths ratio, petiole widths, chilling requirement, bud shape, dormant bud shape, mature leaf color, shape of leaf margin and leaf tip, were chosen to present a new key to mulberry in Taiwan, which is suitable for all sex types.
The ploidy of 26 mulberry accessions was determined by flow cytometer. Accessions of M. bombycis, M. alba, M. latifolia, M. australis, M. formosensis, and M. aropurpurea were diploids with genome sizes ranging from 0.61 to 0.71 pg/2C. Aceesions 70C006 (M. laevigata) was a triploid with a genome size of 1.06 pg/2C. Accession 67C001 (M. australis) comprised diploid and triploid with genome size of 0.63 pg/2C and 0.98 pg/2C, respectively. The morphological characters were bigger in triploid also revealed the difference in ploidy leve.
The karyotypes of 13 accessions were similar. Among the 14 pairs of chromosomes, chromosome 2, 5, 14 are submetacentric chromosomes while the others are metacentric. Fluorescence in situ hybridization of 5S rDNA and 18S-5.8S-25S rDNA in four species also revealed the resemblance among M. species. In M. australis and some mulberry hybrids, one p-arm of choromosome 3 showed dark staining in male but not in female plants. However, the difference in banding pattern between sex types was not observed in M. alba.
en
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dc.description.tableofcontents目錄 Contents
目錄 i
圖目錄 iii
表目錄 iv
摘要 v
Abstract vi
第一章 總論 1
Chapter 1. General introduction
1.1前言 2
1.2桑樹簡介 3
1.3桑屬植物之分類 4
1.4數量分類學 9
1.5桑樹細胞遺傳學之研究 12
1.6結論及試驗假說 15
1.7參考文獻 17
第二章 臺灣桑樹之歧異度分析 24
Chapter 2. Genetic diversity of mulberry (Morus spp.) in Taiwan
2.1摘要 25
2.2前言 26
2.3材料與方法 27
2.4結果 32
2.5討論 43
2.6結論 47
2.7參考文獻 48
2.8 Abstract 51
第三章 桑樹倍數體及DNA含量之鑑定 52
Chapter 3. Determining ploidy and DNA content of mulberry (Morus spp.)
3.1摘要 53
3.2前言 54
3.3材料與方法 55
3.4結果 57
3.5討論 59
3.6結論 65
3.7參考文獻 66
3.8 Abstract 69
第四章 臺灣桑樹之核型分析及rDNA染色圖譜 70
Chapter 4. Karyotype analysis and physical mapping of rDNA of mulberry (Morus spp.) in Taiwan
4.1摘要 71
4.2前言 72
4.3材料與方法 73
4.4結果 76
4.5討論 88
4.6結論 91
4.7參考文獻 92
4.8 Abstract 95
第五章 總結及未來展望 96
附錄 101
dc.language.isozh-TW
dc.title臺灣桑樹種原分析zh_TW
dc.titleGermplasm analysis of mulberry (Morus spp.) in Taiwanen
dc.typeThesis
dc.date.schoolyear97-2
dc.description.degree碩士
dc.contributor.coadvisor楊雯如(Wen-Ju Yang),張哲嘉(Jer-Chia Chang)
dc.contributor.oralexamcommittee鍾美珠(Mei-Chu Chung)
dc.subject.keyword主成份分析,群集分析,流式細胞儀分析,核型分析,螢光原位雜交,zh_TW
dc.subject.keywordPrincipal component analysis,clustering analysis,flow cytometric analysis,karyotypic analysis,fluorescence in situ hybridization (FISH),en
dc.relation.page107
dc.rights.note同意授權(全球公開)
dc.date.accepted2009-07-30
dc.contributor.author-college生物資源暨農學院zh_TW
dc.contributor.author-dept園藝學研究所zh_TW
顯示於系所單位:園藝暨景觀學系

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