請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9043
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 高燕玉 | |
dc.contributor.author | Chih-Chung Tseng | en |
dc.contributor.author | 曾治中 | zh_TW |
dc.date.accessioned | 2021-05-20T20:07:29Z | - |
dc.date.available | 2009-08-14 | |
dc.date.available | 2021-05-20T20:07:29Z | - |
dc.date.copyright | 2009-08-14 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-08-06 | |
dc.identifier.citation | 高玉馨. 2001. 以 5S rDNA 基因間空白區序列探討蝴蝶蘭屬植物之親緣關係. 國立台灣大學植物學研究所碩士論文.
李文靜. 2002. 蝴蝶蘭屬植物核醣體 RNA 基因的選殖及實質定位. 國立台灣大學植物學研究所碩士論文. 彭欣羚. 2007. 利用數種重複性DNA序列的分佈建立朵麗蘭的核型. 國立台灣大學分子與細胞生物學研究所碩士論文. 李益豪. 2007. 核糖體RNA 基因於七種蝴蝶蘭屬植物的分佈. 國立台灣大學分子與細胞生物學研究所碩士論文. 陳怡璇. 2009. 五種蝴蝶蘭屬植物核糖體RNA 基因之染色體定位. 國立台灣大學分子與細胞生物學研究所碩士論文. Appels, R., W. L. Gerlach, E. S. Dennis, et al. 1980. Molecular and chromosomal organization of DNA sequences coding for the ribosomal RNAs in cereals. Chromosoma 78: 293-311. Arends, J. C. 1970. Cytological observations on genome homology in eight interspecific hybrids of Phalaenopsis. Genetica 41: 88-100. Ausubel, F. M., R. Brent, D. D. More, J. G. Sediman, J. A. Smith, and K. Struhl. 1987. Current Protocols in Molecular Biology. Greene Publishing Associates and Wile-Interscience USA. Badaeva, E. D., B. Friebe, and B. S. Gill. 1996. Genome differentiation in Aegilops. 2. Physical mapping of 5S and 18S-26S ribosomal RNA gene families in diploid species. Genome 39: 1150-1158. Brown, S. E., J. L. Stephens, N. L. V. Lapitan, and D. L. Knudson. 1999. FISH landmarks for barley chromosomes (Hordeum vulgare L.). Genome 42: 274-281. Christenson, E. A. 2001. Phalaenopsis: A Monograph. Timber Press, Portland, Oregon. Cox, A. V., A. M. Pridgeon, V. A. Albert, and M. W. Chase. 1997. Phylogenetics of the slipper orchids (Cypripedioideae, Orchidaceae): nuclear rDNA ITS sequences. Plant Syst. Evol. 208: 197-223. Dvorak, J., H. B. Zhang, R. S. Kota, and M. Lassner. 1989. Organization and evolution of the 5S ribosomal RNA gene family in wheat and related species. Genome 32: 1003-1016. Dressler, R. L. 1993. Phylogeny and classification of the orchid family. Cambridge University Press, Cambridge. Ellis, T. N., D. Lee, C. M. Thomas, et al. 1988. 5S rRNA genes in Pisum: sequence, long range and chromosomal organization. Mol. Gen. Genet. 214: 333-342. Flavell, R. B. 1985. Repeated sequences and genome change. In: B. Hohn and E.S. Dennis (eds). Genetic Flux in Plants. Springer-Verlag, Wien, New York. pp. 139-156. Flavell, R. B. 1986. Repetitive DNA and chromosome evolution in plants. Phil. Trans. R. Soc. Lond. B 312: 227-242. Gall, J. G., and M. L. Pardue. 1969. Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc. Natl. Acad. Sci. USA 63: 378-383. Heslop-Harrison, J. S. 1991. The molecular cytogenetics of plants. J. Cell Sci. 100: 15-21. Heslop-Harrison, J. S. 2000. Comparative genome organization in plants: from sequence and markers to chromatin and chromosomes. Plant Cell 12: 617-635. Kao, Y. Y., S. B. Chang, T. Y. LIN, C. H. Hsieh, Y. H. Chen, W. H. Chen, and C. C. Chen. 2001. Differential accumulation of heterochromatin as a cause for karyotype variation in Phalaenopsis orchids. Ann. Bot. 87: 387-395. Lapitan, N. L. V., M. W. Ganal, and S. D. Tanksley. 1991. Organization of the 5S ribosomal RNA genes in the genome of tomato. Genome 34: 509-514. Lapitan, N. L. V. 1992. Organization and evolution of higher plant nuclear genomes. Genome 35: 171-181. Lin, S., H. C. Lee, W. H. Chen, C. C. Chen, Y. Y. kao, Y. M. Fu, Y. H. Chen, and T. Y. Lin. 2001. Nuclear DNA contents of Phalaenopsis sp. and Doritis pulcherrima. J. Amer. Soc. Hort. Sci. 126: 195-199. Mascia, P. N., I. Rubenstein, R. L. Phillips, et al. 1981. Localization of the 5S rRNA genes and evidence for diversity in the 5S rDNA region of maize. Gene 15: 7-20. Mukai, Y., T. R. Endo, and B. S. Gill. 1990. Physical mapping of the 5S rRNA multigene family in common wheat. J. Hered. 81: 290-295. Murata, M., J. S. Heslop-Harrison, and F. Motoyoshi. 1997. Physical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by multi-color fluorescence in situ hybridization with cosmid clones. Plant J. 12: 31-37. Pardue, M. L., S. A. Gerbi, R. A. Eckhardt, J. G. Gall. 1970. Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera. Chromosoma 29: 268-290. Peterson, D. G., H. J. Price, J. S. Johnston, S. M. Stack. 1996. DNA content of heterochromatin and euchromatin in tomato (Lycopersicon esculentum) pachytene chromosomes. Genome 39: 77-82. Sagawa, Y. 1962. Cytological studies of the genus Phalaenopsis. Am. Orchid Soc. Bull. 31: 459-465. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Schwarzacher, T., and P. Heslop-Harrison. 2000. Practical in situ Hybridization. BIOS Scientific Publishers Limited, Oxford, UK. Seijo, J. G., G. I. Lavia, A. Fernandez, A. Krapovickas, D. Ducasse, and E. A. Moscone. 2004. Physical mapping of the 5S and 18S-25S rRNA genes by FISH as evidence that Arachis duranensis and A. ipaensis are the wild diploid progenitors of A. hypogaea (Leguminosae). Am. J. Bot. 91: 1294-1303. Shindo, K., and H. Kamemoto. 1963. Karyotype analysis of some species of Phalaenopsis. Cytologia 28: 390-398. Singh, R. J., H. H. Kim, and T. Hymowitz. 2001. Distribution of rDNA loci in the genus Glycine Willd. Theor. Appl. Genet. 103: 212-218. Srisuwan, S., D. Sihachakr, S. Siljak-Yakovlev. 2006. The origin and evolution of sweet potato (Ipomoea batatas Lam.) and its wild relatives through the cytogenetic approaches. Plant Sci. 171: 424-433. Sweet, H. R. 1980. The genus Phalaenopsis. Day Printing Corp., Pomona, California. Tsai, C. C., S. C. Huang, and C. H. Chou. 2006. Molecular phylogeny of Phalaenopsis Blume (Orchidaceae) based on the internal transcribed spacer of the nuclear ribosomal DNA. Plant Syst. Evol. 256: 1-16. Verma, R. S. 1988. Heterochromatin: Molecular and structural aspects. Cambridge University Press, Cambridge. Woodard, J. W. 1951. Some chromosome numbers in Phalaenopsis. Am. Orchid Soc. Bull. 20: 356-358. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9043 | - |
dc.description.abstract | 屬於 Amboinenses 節之三種蝴蝶蘭原生種 P. fasciata、P. mariae 和 P. pallens 皆具 38 條染色體 (2n = 2x = 38),且均為中型及小型染色體。利用雙標的螢光原位雜交將 rDNA 實質定位的結果,三種蝴蝶蘭皆具一對 5S 及 45S rDNA 基因座,且均位於小型染色體的末端。而屬於同節的 P. lueddemanniana、P. bastianii 及 P. pulchra 在染色體形態、DNA 含量以及 rDNA 基因座的數目皆與此三種蝴蝶蘭相似,顯示此六物種的親緣關係較接近;然而亦屬 Amboinenses 節之 P. amboinensis、P. venosa 和 P. violacea 則染色體較大,DNA 含量亦較高,且具 1~2 對 5S rDNA 基因座和 4~7 對 45S rDNA 基因座,與前六種蝴蝶蘭有顯著差異,故可將此九種蝴蝶蘭分為兩群,此與過去利用 5S rDNA 的 IGS 序列以及 45S rDNA 的 ITS 序列所建立之親緣關係樹的結果相吻合。rDNA 的實質定位提供蝴蝶蘭屬植物親緣關係探討的另一種證據。 | zh_TW |
dc.description.abstract | Phalaenopsis fasciata, P. mariae and P. pallens classified to section Amboinenses had 38 chromosomes (2n = 2x = 38). Their chromosomes were small and medium in size. Physical mapping of rDNA using double-target fluorescence in situ hybridization (FISH) showed that all of these three species possessed one pair of 5S rDNA loci and one pair of 45S rDNA loci, and all of rDNA loci were located in the terminal site of small chromosomes. These results were similar to those of P. lueddemanniana, P. bastianii and P. pulchra. In addition, karyotype and DNA content among these six species were also much the same. It seemed that they were closely related. However, P. amboinensis, P. venosa and P. violacea also belonging to the same section had larger chromosomes and higher DNA content. They contained 1-2 pair of 5S rDNA loci and 4-7 pair of 45S rDNA loci. Therefore, these nine species could be divided into two groups. It was consistent with the previously constructed phylogenetic trees established by intergenic spacer (IGS) region of 5S rDNA and internal transcribed spacer (ITS) region of 45S rDNA. Physical mapping of rDNA provide another evidence for investigating phylogenetic relationships of Phalaenopsis species. | en |
dc.description.provenance | Made available in DSpace on 2021-05-20T20:07:29Z (GMT). No. of bitstreams: 1 ntu-98-R95b43023-1.pdf: 630688 bytes, checksum: f9e4a141dd0cf6bcc08ba7b09127964d (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 中文摘要 i
英文摘要 ii 圖目錄 iv 表目錄 v 壹、前言 1 貳、材料與方法 9 一、植物材料 9 二、根尖染色體觀察 9 三、抽取含 rDNA 選殖體之質體 DNA 12 四、rDNA 探針的製備 12 五、螢光原位雜交 15 1. 染色體的製備 16 2. 前處理與雜交反應 16 3. 清洗 17 4. 訊號偵測 18 5. 訊號擴增 18 6. 對比染色 (counterstaining) 19 7. 螢光顯微鏡影像觀察 19 參、結果 20 ㄧ、染色體的觀察 20 二、螢光原位雜交 20 肆、討論 26 伍、參考文獻 31 | |
dc.language.iso | zh-TW | |
dc.title | 三種蝴蝶蘭屬植物核糖體 RNA 基因的實質定位 | zh_TW |
dc.title | Physical mapping of ribosomal RNA genes in three Phalaenopsis species | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鍾美珠,吳文鑾 | |
dc.subject.keyword | 蝴蝶蘭,螢光原位雜交,rDNA,實質定位,親緣關係, | zh_TW |
dc.subject.keyword | Phalaenopsis,fluorescence in situ hybridization,rDNA,Physical mapping,phylogenetic relationship, | en |
dc.relation.page | 35 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2009-08-06 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
顯示於系所單位: | 分子與細胞生物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf | 615.91 kB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。