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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 于昌平 | zh_TW |
dc.contributor.advisor | Chang-Ping Yu | en |
dc.contributor.author | 楊采儒 | zh_TW |
dc.contributor.author | Cai-Ru Yang | en |
dc.date.accessioned | 2023-09-22T17:10:16Z | - |
dc.date.available | 2023-11-09 | - |
dc.date.copyright | 2023-09-22 | - |
dc.date.issued | 2023 | - |
dc.date.submitted | 2023-08-10 | - |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/90041 | - |
dc.description.abstract | 氮污染是污水處理中一項重要的議題,嚴重影響了數以萬計的生物。生物處理被認為是高效率、低成本且環境友善的處理方法。然而以懸浮態的微生物操作缺乏長期操作穩定性,且細胞難以回收和再利用。透過微生物固定化,能克服上述懸浮態微生物的缺點,並且具有提供大量生物質、避免細胞沖失,以及對有毒物質具有高抵抗性等的優點。
本研究使用的菌株為廣泛存在自然環境中的真菌 Fusarium sp.(菌株 FF 及 FK)。在有氧條件下,FF 及 FK 分別於第 3 天及第 5 天達到 100.0% 的硝酸鹽去除率。在氮源為亞硝酸鹽或氨氮的實驗中,FF 的去除效率亦較高。氮平衡分析結果顯示,FF 的組別中有 55.1% 的氮被轉換為生物質,39.4% 以氣態形式被去除。 FK 的組別則分別為 64.3% 及 30.5%。在 PCR 測試中,FF 僅有 nirK 被擴增,p450nor 未被擴增;而 FK 則是兩個基因都沒被擴增。然而,由於真菌脫硝基因高度多樣化,目前設計出之引子覆蓋率尚不足,因此仍不能排除 FF 及 FK 具備此兩種基因的可能性。 微生物固定化的部分,以整體去氮效果表現較好的菌株 FF 進行擠出型 3D生物列印,將真菌孢子包埋於材料中,達到固定化的目的。固定化之生物材料雖然初期反應速率較懸浮態真菌慢,但於再利用時顯現出高效的脫硝能力。材料能夠留在反應槽內重複利用多次,並在不同環境中保存數天仍具有脫硝能力。整體而言,將真菌以 3D 生物列印技術固定化是可行且效果良好的。 | zh_TW |
dc.description.abstract | Nitrogen pollution is a crucial issue in wastewater treatment, severely impacting on a massive number of organisms. Biological treatment is considered to be an efficient, cost-effective, and environmental-friendly treatment process. However, processes applying suspended microbes lack long-term operational stability, cell recovery, and reusability. Overcoming these drawbacks, microbial immobilization offers several advantages, including abundant biomass, prevention of cell washout, and high resistance to toxic substances.
In this study, widely distributed fungi Fusarium sp. (strains FF and FK) were employed. Under aerobic conditions, FF and FK achieved 100.0% nitrate removal on day 3 and day 5, respectively. In experiments using nitrite or ammonia as nitrogen sources, FF also demonstrated higher removal efficiency. Nitrogen balance analysis revealed that in the FF group, 55.1% of nitrogen was converted into biomass, while 44.9% was released in gaseous form. The corresponding percentages for the FK group were 64.3% and 35.7%, respectively. In the PCR test, only nirK was amplified in FF, while p450nor was not amplified. As for FK, neither of the two genes was amplified. However, due to the high diversity of fungal denitrification genes and the limited coverage of primers, the possibility of FF and FK containing these genes cannot be ruled out. In the microbial immobilization process, strain FF, which demonstrates superior overall nitrogen removal efficiency, is employed for extrusion-based 3D bioprinting. Fungal spores are entrapped within the material to achieve immobilization. Although the immobilized material initially exhibited a slower removal rate compared to suspended fungi, it demonstrated high denitrification efficiency upon reuse. The material can be retained within the reactor and reused multiple times, as well as maintaining denitrification capabilities even after several days of storage in various environments. On the whole, the immobilization of fungi by 3D bioprinting is feasible and effective. | en |
dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-09-22T17:10:16Z No. of bitstreams: 0 | en |
dc.description.provenance | Made available in DSpace on 2023-09-22T17:10:16Z (GMT). No. of bitstreams: 0 | en |
dc.description.tableofcontents | 口試委員會審定書 i
誌謝 iii 摘要 v ABSTRACT vii 目錄 ix 圖目錄 xiii 表目錄 xv 第一章 緒論 1 1.1 研究背景 1 1.2 研究動機 2 1.3 研究目的 3 第二章 文獻回顧 5 2.1 真菌 5 2.1.1 Fusarium solani 及 Clonostachys rosea 5 2.1.2 真菌修復 6 2.2 真菌脫硝作用 8 2.2.1 微生物脫硝作用 8 2.2.2 真菌脫硝途徑 9 2.3 微生物固定化 12 2.3.1 微生物固定化應用於生物修復 12 2.3.2 微生物固定化種類 12 2.3.3 真菌的微生物固定化應用於廢水處理 14 2.4 3D生物列印 16 第三章 材料與方法 19 3.1 實驗藥品與設備 19 3.1.1 實驗用藥品 19 3.1.2 實驗儀器與設備 22 3.1.3 研究流程圖 24 3.2 真菌培養 25 3.2.1 真菌菌種來源 25 3.2.2 培養基 25 3.2.3 真菌繼代及培養 26 3.2.4 真菌孢子懸浮液製備 27 3.2.5 混合液懸浮固體 (Mixed Liquor Suspended Solids, MLSS) 分析 28 3.3 真菌去氮能力試驗 29 3.3.1 真菌脫硝能力試驗 29 3.3.2 真菌去除氨氮能力試驗 29 3.3.3 離子層析 30 3.3.4 氨氮分析 31 3.3.5 總有機碳及總氮分析 32 3.4 氮平衡分析 33 3.4.1 氮平衡分析實驗方法 33 3.4.2 元素分析 33 3.5 基因檢測 34 3.5.1 去氧核醣核酸 (Deoxyribonucleic Acid, DNA) 萃取 34 3.5.2 聚合酶連鎖反應 (Polymerase Chain Reaction, PCR) 35 3.5.3 膠體電泳 37 3.5.4 PCR產物定序 37 3.6 3D 生物列印材料 38 3.6.1 3D 生物列印機及列印參數 38 3.6.2 生物墨水配製 39 3.6.3 材料列印步驟 40 3.7 3D生物列印材料應用 42 3.7.1 3D生物列印材料脫硝能力試驗 42 3.7.2 3D生物列印材料保存測試 42 3.7.3 3D 生物列印材料再利用測試 42 3.7.4 材料表面型態分析 43 第四章 結果與討論 45 4.1 真菌培養及觀察 45 4.1.1 真菌型態觀察 45 4.1.2 菌株去氮能力初步篩選 46 4.1.3 真菌菌種鑑定 48 4.1.4 MLSS-OD6oonm 曲線 50 4.2 真菌去氮能力試驗 51 4.2.1 硝酸鹽去除試驗 51 4.2.2 亞硝酸鹽去除試驗 55 4.2.3 氨氮去除試驗 58 4.2.4 不同碳氮比條件下去氮能力試驗 61 4.3 氮平衡分析 64 4.4 基因檢測 66 4.5 3D 生物列印材料 67 4.5.1 3D 生物列印材料調整 67 4.5.2 3D 生物列印材料去氮能力試驗 69 4.5.3 3D 生物列印材料再利用測試 71 4.5.4 3D 生物列印材料保存測試 73 4.5.5 材料型態 75 4.5.6 材料表面型態分析 78 第五章 結論與建議 81 5.1 結論 81 5.2 建議 83 參考文獻 85 附錄 101 | - |
dc.language.iso | zh_TW | - |
dc.title | 真菌在去氮上的研究及其在3D生物列印微生物固定化的應用 | zh_TW |
dc.title | Nitrogen Removal by Fungi and Their Application in 3D Bioprinting for Microbial Immobilization | en |
dc.type | Thesis | - |
dc.date.schoolyear | 111-2 | - |
dc.description.degree | 碩士 | - |
dc.contributor.oralexamcommittee | 林居慶;郭獻文 | zh_TW |
dc.contributor.oralexamcommittee | Chu-Ching Lin;Hsion-Wen Kuo | en |
dc.subject.keyword | Fusarium,真菌脫硝作用,除氮,微生物固定化,3D 生物列印, | zh_TW |
dc.subject.keyword | Fusarium,fungal denitrification,nitrogen removal,microbial immobilization,3D bioprinting, | en |
dc.relation.page | 103 | - |
dc.identifier.doi | 10.6342/NTU202303177 | - |
dc.rights.note | 同意授權(全球公開) | - |
dc.date.accepted | 2023-08-11 | - |
dc.contributor.author-college | 工學院 | - |
dc.contributor.author-dept | 環境工程學研究所 | - |
顯示於系所單位: | 環境工程學研究所 |
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