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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝旭亮 | zh_TW |
| dc.contributor.advisor | Hsu-Liang Hsieh | en |
| dc.contributor.author | 顏嘉成 | zh_TW |
| dc.contributor.author | Chia-Cheng Yen | en |
| dc.date.accessioned | 2023-09-22T17:04:18Z | - |
| dc.date.available | 2023-11-09 | - |
| dc.date.copyright | 2023-09-22 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-08-11 | - |
| dc.identifier.citation | Cantón F R, Quail P H (1999) Both phya and phyb mediate light-imposed repression of phya gene expression in arabidopsis. Plant Physiol 121: 1207-1216
Cecchetti V, Brunetti P, Napoli N, Fattorini L, Altamura M M, Costantino P, Cardarelli M (2015) Abcb1 and abcb19 auxin transporters have synergistic effects on early and late arabidopsis anther development. J Integr Plant Biol 57: 1089-1098 Chen E, Swartz T E, Bogomolni R A, Kliger D S (2007) A lov story: The signaling state of the phot1 lov2 photocycle involves chromophore-triggered protein structure relaxation, as probed by far-uv time-resolved optical rotatory dispersion spectroscopy. Biochemistry 46: 4619-4624 Chen H J, Chen C L, Hsieh H L (2015) Far-red light-mediated seedling development in arabidopsis involves far-red insensitive 219/jasmonate resistant 1-dependent and -independent pathways. PLoS One 10: e0132723 Chen H J, Fu T Y, Yang S L, Hsieh H L (2018) Fin219/jar1 and cryptochrome1 antagonize each other to modulate photomorphogenesis under blue light in arabidopsis. PLoS Genet 14: e1007248 Christie J M, Yang H, Richter G L, Sullivan S, Thomson C E, Lin J, Titapiwatanakun B, Ennis M, Kaiserli E, Lee O R, et al. (2011) Phot1 inhibition of abcb19 primes lateral auxin fluxes in the shoot apex required for phototropism. PLoS Biol 9: e1001076 Friml J, Wiśniewska J, Benková E, Mendgen K, Palme K (2002) Lateral relocation of auxin efflux regulator pin3 mediates tropism in arabidopsis. Nature 415: 806-809 Harada A, Takemiya A, Inoue S, Sakai T, Shimazaki K (2013) Role of rpt2 in leaf positioning and flattening and a possible inhibition of phot2 signaling by phot1. Plant Cell Physiol 54: 36-47 Hsieh H L, Okamoto H, Wang M, Ang L H, Matsui M, Goodman H, Deng X W (2000) Fin219, an auxin-regulated gene, defines a link between phytochrome a and the downstream regulator cop1 in light control of arabidopsis development. Genes Dev 14: 1958-1970 Jiang H W, Peng K C, Hsu T Y, Chiou Y C, Hsieh H L (2023) Arabidopsis fin219/jar1 interacts with phytochrome a under far-red light and jasmonates in regulating hypocotyl elongation via a functional demand manner. PLoS Genet 19: e1010779 Küpers J J, Oskam L, Pierik R (2020) Photoreceptors regulate plant developmental plasticity through auxin. Plants (Basel) 9 Kimura T, Haga K, Sakai T (2022) The phosphorylation status of nonphototropic hypocotyl3 affects phot2-dependent phototropism in arabidopsis. Plant Signal Behav 17: 2027138 Kinoshita T, Doi M, Suetsugu N, Kagawa T, Wada M, Shimazaki K (2001) Phot1 and phot2 mediate blue light regulation of stomatal opening. Nature 414: 656-660 Legris M, Szarzynska-Erden B M, Trevisan M, Allenbach Petrolati L, Fankhauser C (2021) Phototropin-mediated perception of light direction in leaves regulates blade flattening. Plant Physiol 187: 1235-1249 Mier P, Alanis-Lobato G, Andrade-Navarro M A (2017) Protein-protein interactions can be predicted using coiled coil co-evolution patterns. J Theor Biol 412: 198-203 Paik I, Huq E (2019) Plant photoreceptors: Multi-functional sensory proteins and their signaling networks. Seminars in Cell & Developmental Biology 92: 114-121 Pedmale U V, Liscum E (2007) Regulation of phototropic signaling in arabidopsis via phosphorylation state changes in the phototropin 1-interacting protein nph3*. Journal of Biological Chemistry 282: 19992-20001 Peng K C, Siao W, Hsieh H L (2023) Far-red insensitive 219 and phytochrome b corepress shade avoidance via modulating nuclear speckle formation. Plant Physiol 192: 1449-1465 Reuter L, Schmidt T, Manishankar P, Throm C, Keicher J, Bock A, Droste-Borel I, Oecking C (2021) Light-triggered and phosphorylation-dependent 14-3-3 association with non-phototropic hypocotyl 3 is required for hypocotyl phototropism. Nat Commun 12: 6128 Roberts D, Pedmale U V, Morrow J, Sachdev S, Lechner E, Tang X, Zheng N, Hannink M, Genschik P, Liscum E (2011) Modulation of phototropic responsiveness in arabidopsis through ubiquitination of phototropin 1 by the cul3-ring e3 ubiquitin ligase crl3(nph3). Plant Cell 23: 3627-3640 Ruan J, Zhou Y, Zhou M, Yan J, Khurshid M, Weng W, Cheng J, Zhang K (2019) Jasmonic acid signaling pathway in plants. Int J Mol Sci 20 Sullivan J A, Deng X W (2003) From seed to seed: The role of photoreceptors in arabidopsis development. Developmental Biology 260: 289-297 Swain S, Jiang H W, Hsieh H L (2017) Far-red insensitive 219/jar1 contributes to shade avoidance responses of arabidopsis seedlings by modulating key shade signaling components. Front Plant Sci 8: 1901 Zhai S, Cai W, Xiang Z X, Chen C Y, Lu Y T, Yuan T T (2021) Pin3-mediated auxin transport contributes to blue light-induced adventitious root formation in arabidopsis. Plant Sci 312: 111044 Zhang Q, Gong M, Xu X, Li H, Deng W (2022) Roles of auxin in the growth, development, and stress tolerance of horticultural plants. Cells 11 朱彥樺. (2020). 阿拉伯芥中透過去磷酸酶調節FIN219/JAR1活性並影響藍光及茉莉酸訊息傳遞之功能性研究。國立台灣大學碩士論文。 陳佩吟. (2015). 阿拉伯芥中FIN219在向光性反應之功能性研究。國立台灣大學碩士論文。 彭凱駿. (2023). Far-red insensitive 219/jar1與phytochrome b透過調控核斑型態共同抑制阿拉伯芥之遮蔭反應。國立台灣大學博士論文。 | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/90017 | - |
| dc.description.abstract | 在阿拉伯芥中，FIN219 (FAR-RED INSENSITIVE 219)會協助生合成具有生物活性的茉莉酸 (JA-Ile)，是植物茉莉酸參與防禦反應與調控植物生長發育中十分重要的角色。植物的向光性反應主要受到藍光的光接受者PHOTOTROPINS (PHOTs)與其下游的訊息傳遞者NON-PHOTOTROPIC HYPOCOTYL3 (NPH3)所調節。在先前研究發現缺乏FIN219似乎會影響阿拉伯芥的向光性反應。為了更加了解FIN219是如何參與向光性反應機制，因此首先透過fin219-2突變株在接收到側邊藍光的照射後，發現表現出向光性缺失的外表型。我們透過Semi-CoIP assay 和雙分子螢光互補系統(BiFC)實驗證明了，PHOT1與FIN219蛋白質之間在細胞質與細胞膜有著交互作用的關係。並且透過Pull-down assay、Co-IP與BiFC也證明了NPH3與FIN219蛋白質之間也有著交互作用的現象。有趣的是，透過西方墨點法(Western blot)實驗發現，phot1、phot1/2與nph3突變株中FIN219蛋白質的表現量有明顯降低，說明在藍光所調節的向光性反應中光接受者PHOT1與下游的訊息傳遞者NPH3會正向調控FIN219，而PHOT1會穩定NPH3與FIN219之間的交互作用。當缺乏FIN219時NPH3無法正常去磷酸化，進而出現向光性缺失的情況。總之，FIN219在PHOT1，NPH3引發的阿拉伯芥向光性反應中扮演重要的角色。 | zh_TW |
| dc.description.abstract | In Arabidopsis, FIN219 (FAR-RED INSENSITIVE 219) participates in the biosynthesis of biologically active jasmonic acid-isoleucine (JA-Ile), playing a crucial role in plant defense response and plant growth and development. The phototropic response in plants is mainly regulated by the blue light photoreceptors known as PHOTOTROPINS (PHOTs), and their downstream signaling component NON-PHOTOTROPIC HYPOCOTYL3 (NPH3). Previous studies indicated that the absence of FIN219 seems to affect the phototropic response in Arabidopsis. To gain further insights into the interaction between FIN219 and the phototropic response mechanism, we firstly observed a defect of phototropism phenotype in the fin219-2 mutant upon exposure to lateral blue light. Through Semi-CoIP assay and Bimolecular Fluorescence Complementation (BiFC), we demonstrated the interaction between PHOT1 and FIN219 protein in the cytoplasm and cell membrane. Furthermore, Pull-down assay, CoIP, and BiFC experiments showed the interaction between NPH3 and FIN219. Interestingly, protein immunoblot analysis (Western blot) revealed a significant reduction in the expression level of FIN219 protein in phot1, phot1/2, and nph3 mutants, suggesting that the upstream blue light photoreceptor PHOT1 and downstream signaling component NPH3 positively regulate FIN219. Moreover, PHOT1 stabilizes the interaction between NPH3 and FIN219. In the absence of FIN219, NPH3 fails to undergo proper dephosphorylation, resulting in a loss of phototropism phenotype. Taken together, FIN219 plays a vital role in phot1-NPH3-mediated phototropic response in Arabidopsis. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-09-22T17:04:18Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-09-22T17:04:18Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 致謝II
中文摘要III AbstractIV Table of ContentsVI List of Figures1 Introduction2 1. Light signaling in Arabidopsis thaliana2 2. Phototropic responses regulated by Phototropins3 3. The relationship between AUXIN signaling pathway and phototropism5 4. Jasmonate signaling pathway6 5. FIN219 is involved in the regulation of photoreceptor7 6.The motivation and purpose of this study8 Materials and methods9 1. Plant material9 2. Quantification of phototropic response9 3. Histochemical GUS staining10 4. Expression and purification of recombinant protein11 5.Bimolecular Fluorescence Complementation (BiFC) Assay12 6. Extraction of plant protein and analyze by western blot13 Results16 1.fin219-2 mutant exhibits a defect in phototropism after exposure to unilateral blue light.16 2. The expression patterns of DR5:GUS and pFIN219:GUS in response to unilateral blue light are different.16 3. FIN219 interacts with NPH3 and PHOT1 in In vitro assay.18 4. FIN219 interacts with NPH3 and PHOT1 in In vitro assay.18 5. The interaction between NPH3 and FIN219 in Arabidopsis seedlings under the dark and low blue light conditions.19 6. PHOT1 affects the interaction between NPH3 and FIN219 20 7. FIN219 affects the activity of NPH3-YFP under blue light20 8. FIN219 affects the phosphorylation status of NPH3 protein.21 9. 35S:NPH3/fin219-2 and fin219-2 mutant shows similar phenotype.22 Discussion23 1. The relationship between jasmonic acid defense response and phototropic response.23 2. Differences in expression between DR5:GUS and pFIN219:GUS24 3. Interaction of FIN219, NPH3 and PHOT124 4. Phenotypes of 35S:NPH3/nph3 and 35S:NPH3/fin219-225 5. FIN219 participates in the phototropic response primarily regulated by PHOT1 through its interaction with NPH3.25 Figures26 References39 Table 1 44 | - |
| dc.language.iso | en | - |
| dc.subject | 向光性反應 | zh_TW |
| dc.subject | NPH3 | zh_TW |
| dc.subject | 藍光 | zh_TW |
| dc.subject | FIN219 | zh_TW |
| dc.subject | PHOT1 | zh_TW |
| dc.subject | FIN219 | en |
| dc.subject | PHOT1 | en |
| dc.subject | Blue light | en |
| dc.subject | NPH3 | en |
| dc.subject | Phototropism | en |
| dc.title | 阿拉伯芥中FIN219透過與NPH3交互作用參與向光性反應之功能性研究 | zh_TW |
| dc.title | Functional Study of FIN219 and NPH3 Interaction in Arabidopsis Phototropism | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 李金美;鄭梅君;邱崇益 | zh_TW |
| dc.contributor.oralexamcommittee | Chin-Mei Lee;Mei-Chun Cheng;Chung-Yi Chiou | en |
| dc.subject.keyword | 向光性反應,藍光,NPH3,FIN219,PHOT1, | zh_TW |
| dc.subject.keyword | NPH3,FIN219,PHOT1,Blue light,Phototropism, | en |
| dc.relation.page | 45 | - |
| dc.identifier.doi | 10.6342/NTU202302989 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2023-08-12 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 植物科學研究所 | - |
| dc.date.embargo-lift | 2028-08-08 | - |
| 顯示於系所單位： | 植物科學研究所 | |
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