添加氯化鋅改質以氫氧基磷灰石為基底的光固化活髓治療生物材料

Abstract

齲齒是一種常見的口腔疾病，由於人類牙釉質無法再生，牙本質生長速度緩慢，一旦遭細菌感染，若未能及早發現治療，將導致牙齒走向根管治療的結果。根管治療後牙齒使用性能將大不如前，因此齲齒治療上，以盡可能保留牙髓活性作為治療方向。覆髓材料是一種在治療過程中直接覆蓋在牙髓上，將其與外界阻隔、保護及維持牙髓活性的材料。 目前在牙科材料發展上，為改善以生物性水泥為基底的覆髓材料問題，所研發出來的光聚合樹脂，有效縮短了聚合時間並提高了操作的便利性，但其固化過程所釋放的溫度有可能對牙髓造成不可逆的損傷。 過去本實驗室以光聚合的覆髓材料概念為方向研發出一款由磷酸三鈣(Tricalcium phosphate)及氫氧基磷灰石(Hydroxyapatite)為基底再添加光聚合物樟腦醌(Camphorquinone 6-Oxocamphor)所混合出一款光聚合樹脂，並期望相較於市售光聚合樹脂，其固化過程中的溫度釋放能減少。 本研究以此材料為基底並添加氯化鋅(ZnCl2)，評估添加不同濃度氯化鋅後的光聚合樹脂物理性質及生物應用上的影響。實驗結果證實添加氯化鋅的材料能降低固化時的溫度。以細胞活性測試劑Thiazolyl blue tetrazolium bromide確認材料與人類牙髓幹細胞有良好的生物相容性，以茜素紅S染色法(Alizarin Red S Stain Assay, ARS Assay)及聚合酶連鎖反應分析 (Polymerase chain reaction, PCR Assay)證實覆髓材料添加氯化鋅後相較於市售光聚合樹脂能促進人類牙髓幹細胞分化和加速光聚合時間及降低光照5秒內之溫度變化，由本實驗所製作出之新款覆髓材料未來將可應用於人類齲齒治療。

Dental caries is a common oral disease. Because enamel cannot regenerate and the growth of reparative dentin is slow, bacterial infection of tooth without early treatment. Function of teeth would decrease after root canal treatment. Therefore, it is very important to the vitality of dentin pulp in tooth treatment. Pulp-capping materials is therefore used for this purpose. Pulp-capping material is a material that directly covers the pulp during treatment to block, protect and maintain the activity of the pulp. Light-activated resin was developed to improve the defects of resin base on biological cement. Although it effectively reduces the curing time and more convenient in operation, the high temperature release during the curing process cause irreversible damage to the dentin pulp stem cell is possible.In the past, the researchers in our laboratory develop a new kind of Light-activated resin which base on the Tricalcium phosphate, Hydroxyapatite and Camphorquinone 6-Oxocamphor. The group tried to develop material with less temperature after curing process. In this research, according to the previous developed, material and the various ZnCl2 contents, then were added to evaluate the properties of materials. We found that added with ZnCl2 the temperature release of material after curing process was decrease. Our results demonstrated the material with good biocompatibility with DPSC by using cell viability test we also found that the material can induce DPSC differentiation and mineralization by using ARS Assay and PCR Assay. In this experiment, we development a new Light-activated capping material and hope it will be used for vital pulp therapy.