NURECON：利用宏基因體資料建立新型營養攝取需求線上系統

Abstract

飲食習慣影響著人類腸道微生物組成。在過去的數十年間，研究已發現腸道微生物可以利用食物消化後的營養素來產生代謝物，與人類生理有著密切關係，也因此腸道菌叢與營養素對人體健康的影響顯得相當重要。然而，至今鮮少有研究著重於利用腸道菌相預測出缺乏的營養素，想要透過文獻尋找基準群體菌相與手邊研究資料進行比較，其過程也相當困難與繁瑣。這份論文提出一款線上分析平台 - NURECON：藉由整合次世代定序微生物 16S 核糖體 RNA 分析流程（QIIME2）、代謝途徑預測工具（PICRUSt2 與 KEGG）以及食物與化合物資料庫（FooDB），預測個人缺乏的營養素並提供飲食建議。該平台從已發表的文獻中，收錄 287 位健康人的腸道菌相，用以建立基準微生物菌相與代謝物組成。NURECON 可以讓使用者上傳自己的 16S 序列資料進行分析，上傳的序列資料將會與平台中的基準群體進行分析及比較，預測缺乏的營養素。最終以視覺化圖表的方式呈現腸道菌相組成、相關的酵素及代謝物分析結果。NURECON 是一款使用者友善便捷的線上平台，其所提供的營養素建議能夠輔助營養師設計食譜或作為研究時的參考依據。本系統網址為：https://nurecon.cgm.ntu.edu.tw/。

Dietary habits have been proven to have an impact on the microbial composition and health of the human gut. Over the past decade, researchers have discovered that gut microbiota can use nutrients to produce metabolites that have major implications for human physiology. However, there is no comprehensive system that specifically focuses on identifying nutrient deficiencies based on gut microbiota, making it difficult to interpret and compare gut microbiome data in the literature. This study proposes an analytical platform, NURECON, that can predict nutrient deficiency information in individuals by comparing their metagenomic information to a reference baseline. NURECON integrates a next-generation bacterial 16S rRNA analytical pipeline (QIIME2), metabolic pathway prediction tools (PICRUSt2 and KEGG), and a food compound database (FooDB) to enable the identification of missing nutrients and provide personalized dietary suggestions. Metagenomic information from total number of 287 healthy subjects was used to establish baseline microbial composition and metabolic profiles. Users can upload their 16S sequence data to NURECON for analysis. The uploaded data is analyzed and compared to the baseline for nutrient deficiency assessment. Visualization results include gut microbial composition, related enzymes, and nutrient abundance. NURECON is a user-friendly online platform that provides nutritional advice to support dietitians’ research or menu design. The system is freely available at https://nurecon.cgm.ntu.edu.tw/.