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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 洪傳揚 | |
| dc.contributor.author | Yun-Ting Kao | en |
| dc.contributor.author | 高筠婷 | zh_TW |
| dc.date.accessioned | 2021-05-20T20:03:20Z | - |
| dc.date.available | 2014-08-21 | |
| dc.date.available | 2021-05-20T20:03:20Z | - |
| dc.date.copyright | 2009-08-21 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-18 | |
| dc.identifier.citation | Abuqamar S, Luo H, Laluk K, Mickelbart MV, Mengiste T (2009) Crosstalk between biotic and abiotic stress responses in tomato is mediated by the AIM1 transcription factor. Plant J 58: 347-360
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8885 | - |
| dc.description.abstract | 在植物中Ascorbate peroxidase是一個關鍵的抗氧化酵素，可將細胞中H2O2轉變成水。水稻中共有8個APx基因，其中兩個位於細胞質、兩個位於peroxisome，四個表現在葉綠體中，然而水稻APx基因對環境外在刺激的反應仍未清楚研究。為廣泛了解APx基因表現模式，以即時定量PCR來分析APx基因表現，我們的結果顯示水稻多數的APx基因大量累積在幼苗地上部組織，在重金屬處理可誘導水稻根部APx1與APx3誘導10倍以上表現量，另外幾乎所有的APx基因都被缺水、鹽分及ABA處理誘導而大量在根部及地上部表現。植物荷爾蒙處理顯示除了APx7外，水楊酸顯著的在根部誘導APx基因表現。以次細胞蛋白定位分析OsAPx1及OsAPx2位置，結果顯示OsAPx1與OsAPx2皆在細胞質和細胞核中表現。我們過去的研究顯示，水稻APx8會受鹽分誘導表現，為進一步了解其在鹽分逆境下之扮演的角色，試驗中分析水稻APx8啟動子驅動GUS報導基因之轉殖水稻，結果顯示GUS在葉片、葉鞘、莖部、內外穎、種子胚乳及胚中累積，刻傷及鹽分處理也提高GUS在葉片中的累積。分析APx8基因嵌入突變株osapx8的活性，結果顯示地上部減少40%的APx活性，鹽分處理下也呈現較低的誘導性。在鹽分耐受性測試中，osapx8表現對鹽分敏感之外表型，以上結果顯示OsAPx8是一個在鹽分逆境下具有保護植物功能的重要基因。 | zh_TW |
| dc.description.abstract | In plants, ascorbate peroxidase is a key antioxidative enzyme which catalyze the conversion of H2O2 to H2O. In rice, APx gene family is composed of eight genes, including two cytosolic isofoms, two putative peroxisomal isoforms, and four chloroplastic isoforms, and yet their gene expression patterns response to external stimuli remain mostly uncharacterized. To comprehensively understand the specific gene expression patterns of each individual of the APx gene, quantitative real-time PCR was conducted. Our results showed that most transcripts of APx genes are highly accumulated in the shoot tissue. The heavy metal response of APx indicated that more than 10 folds of APx1 and APx3 transcripts were induced by copper in root. Furthermore, almost all APx gene were significantly enhanced by dehydration, salt, and ABA either in root or shoot. Phytohormones treatments revealed that salicyclic acid notably increased the expression of all APx genes, except APx7, in root. Subcellular localization analysis demonstrated that both cytosolic isoform OsAPx1 and OsAPx2 are localized at cytosol and nucleus. Our previous study showed that OsAPx8 is highly induced by salt stress in root. To further understand its roles in salt stress, PAPx8::GUS transgenic rice was generated. PAPx8::GUS transgenic plants showed that GUS accumulated in blade, sheath, stem, lemma, palea, endosperm and embryo. Wounding and salt stress enhanced the accumulation of GUS protein in leaf. Analysis of APx8 knockout mutant, osapx8, showed that APx activity decreased 40% in leaf, while lower inducibility could be observed upon salt treatment. Salt tolerant assay indicated that osapx8 showed a salt sensitive phenotype. All these data let us conclude that OsAPx8 is a crucial gene in protecting rice from salt stress. | en |
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| dc.description.tableofcontents | 目錄
口試委員會審定書 ..............................................................................................I 誌謝 ....................................................................................................................II 目錄 ....................................................................................................................III 附圖附表目錄 .....................................................................................................IV 圖目錄 ................................................................................................................V 表目錄 ................................................................................................................VI 中文摘要 ............................................................................................................VII 英文摘要 ............................................................................................................VIII 縮寫字對照表 .....................................................................................................X 壹、緒論 一、非生物性逆境及氣候變遷造成全球糧食危機 ...........................................1 二、植物遭遇逆境的調節及保護機制 .............................................................2 三、植物面對逆境的調控機制 ........................................................................6 四、Ascorbate peroxidase之研究 .................................................................8 貳、研究目的 ....................................................................................................14 參、材料與方法 一、基因表現分析 .........................................................................................15 二、蛋白質次細胞位置分析 ...........................................................................18 三、OsAPx8啟動子特性分析 ........................................................................21 四、OsAPx8功能性分析 ...............................................................................24 五、補充資料 .................................................................................................27 肆、結果 一、水稻APx基因家族分子特性分析 ............................................................32 二、水稻APx8基因功能性分析 .....................................................................37 伍、討論 一、水稻APx基因家族分子特性分析與基因表現綜合比較 ............................42 二、水稻OsAPx在兩種對重金屬耐受性不同品種之差異性比較 ....................45 三、OsAPx蛋白質於水稻次細胞位置分析討論 ..............................................47 四、PAPx8/GUS轉殖植物組織專一性及逆境誘導性之比較 ..............................47 五、水稻OsAPx8之功能討論 ........................................................................48 陸、引用文獻 .....................................................................................................50 附表1、阿拉伯芥中APx基因調控及功能之研究 ...............................................58 附表2、水稻及大麥中APx基因調控及功能之研究 ...........................................60 附圖1 、水稻APx胺基酸保守性序列示意圖 ....................................................61 圖1、APx基因家族親緣演化分析。........................................................................ 62 圖2、水稻OsAPx1至OsAPx4基因上游2kb序列順式作用元件分析。................ 63 圖3、水稻OsAPx5至OsAPx8基因上游2kb序列順式作用元件分析。................ 64 圖4、APx基因家族在水稻中之組織專一性表現分析。.......................................... 65 圖5、TNG67水稻地上部在鎘、銅、鋅處理下APx家族之基因表現。................. 66 圖6、TNG67水稻根部在鎘、銅、鋅處理下APx家族之基因表現。................... 67 圖7、TN1水稻地上部在鎘、銅、鋅處理下APx家族之基因表現。...................... 68 圖8、TN1水稻根部在鎘、銅、鋅處理下APx家族之基因表現。.......................... 69 圖9、TNG67水稻地上部在缺水、高鹽逆境及ABA處理下APx家族之基因表現。...70 圖10、TNG67水稻根部在缺水、高鹽逆境及ABA處理下APx家族之基因表現。...71 圖11、TNG67水稻地上部在植物荷爾蒙處理下APx家族之基因表現。................72 圖12、TNG67水稻根部在植物荷爾蒙處理下APx家族之基因表現。....................73 圖13、TNG67水稻葉片刻傷處理後APx家族基因表現。....................................... 74 圖14、水稻OsAPx1及OsAPx2次細胞位置分析。................................................ 75 圖15、PAPx8/GUS轉殖水稻組織化學分析。.............................................................76 圖16、PAPx8/GUS轉殖水稻之組織化學分析。........................................................ 77 圖17 、PosAPx8/GUS幼苗鹽份處理下GUS染色。...................................................78 圖18、PAPx8-GUS轉殖水稻在鹽份逆境及刻傷逆境下之組織化學分析。...............79 圖19、成熟葉片在短時間刻傷逆境下之OsAPx8基因表現。.................................80 圖20、osapx8突變株之分析。................................................................................81 圖21、以即時定量聚合酶方式分析水稻8個APx基因於osapx8中之表現量。..82 圖22、osapx8突變株在鹽份逆境處理下APx及CAT活性測試。.......................... 83 圖23、osapx8突變株逆境耐受性測試。................................................................ 84 圖24、oe-osapx8逆境耐受性測試。 ..................................................................85 表1、水稻TNG67及TCN1在重金屬處理下地上部APx基因表現。......................86 表2、TNG67及TCN1在重金屬處理下根部APx基因表現。................................. 87 表3、兩週大TNG67水稻在非生物性逆境下地上部APx基因表現。.................... 88 表4、兩週大TNG67水稻在非生物性逆境下根部APx基因表現。.........................89 表5、兩週大台農67號水稻地上部在植物荷爾蒙處以下APx家族之基因表現。..90 表6、兩週大台農67號水稻根部在植物荷爾蒙處下APx家族之基因表現。........91 表7、論文使用引子列表 .......................................................................................92 | |
| dc.language.iso | zh-TW | |
| dc.title | 水稻Ascorbate peroxidase基因家族功能分析
1. 水稻Ascorbate peroxidase基因家族之特性及表現 2. 水稻Ascorbate peroxidase 8 (OsAPx8)的功能分析 | zh_TW |
| dc.title | Functional studies of rice ascorbate peroxidase gene family
1. Characterization and expression of rice ascorbate peroxidase gene family 2. Functional analysis of the rice ascorbate peroxidase 8 (OsAPx8) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 高景輝,鄭石通,張孟基,葉靖輝 | |
| dc.subject.keyword | 非生物性逆境,活性氧族,基因表現,功能分析, | zh_TW |
| dc.subject.keyword | ascorbate peroxidase,abiotic stress,reactive oxygen species,gene expression,functional analysis, | en |
| dc.relation.page | 93 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2009-08-18 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| 顯示於系所單位： | 農業化學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-98-1.pdf | 5.77 MB | Adobe PDF | 檢視/開啟 |
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