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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王俊能 | zh_TW |
| dc.contributor.advisor | Chun-Neng Wang | en |
| dc.contributor.author | 陳存瑩 | zh_TW |
| dc.contributor.author | Tsun-Ying Chen | en |
| dc.date.accessioned | 2023-08-15T17:30:07Z | - |
| dc.date.available | 2023-11-09 | - |
| dc.date.copyright | 2023-08-15 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-08-04 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/88719 | - |
| dc.description.abstract | 兩側對稱的花朵能夠藉由限制專一傳粉者拜訪促進物種豐度，因此在被子植物中具有演化優勢。過去在金魚草（Antirrhinum）的研究中，發現CYCLOIDEA（CYC）基因能夠透過調控MYB轉錄因子家族提供背側花瓣特徵，並在花瓣建立背腹側極性，然而還有哪些下游轉錄因子參與其中尚不清楚。大岩桐（Sinningia speciosa ‘Espirito santo’）為兩側對稱花，且有較大的背側花瓣與較小的腹側花瓣。分析大岩桐背腹側花瓣轉錄體後，找出許多生長素訊息傳遞路徑相關基因（如生長素響應因子ARF3，AUXIN RESPONSE FACTOR 3）在背側花瓣高度表現，顯示生長素的調節可能與花瓣背腹側極性建立有關。我利用液向層析串聯式質譜儀在背側花瓣中偵測到較高含量的內生性生長素，並將花苞處理生長素抑制劑後，觀察到花瓣生長受到影響，如背側花瓣的長度縮短。經由雙螢光素酶證實SsARF3與SsCYC間有正向相互促進的關係。為了驗證SsARF3的基因功能，我透過比較阿拉伯芥轉殖株的性狀確認SsARF3為抑制子，在過度表現SsARF3-GFP與SsARF3-SRDX嵌合蛋白之轉植株中，皆有著較大的花瓣面積、較大的花瓣細胞，與縮短的初級花序。這些結果暗示生長素與SsARF3可能與SsCYC合作影響大岩桐背腹花瓣建立。 | zh_TW |
| dc.description.abstract | Floral zygomorphy (bilateral symmetry) prevails in angiosperms and greatly enhances specific pollinators visits, thus promoting species richness. In Antirrhinum, the CYCLOIDEA (CYC) gene established zygomorphy by providing dorsal petal identity, but the downstream regulation except the MYB-like gene family remains unknown. Wild-type Sinningia speciosa ‘Espírito santo’ (Gloxinia) is zygomorphic by having its dorsal petals larger than the ventral petals. From a petal transcriptomic analysis in S. speciosa, most auxin signaling pathway genes, such as AUXIN RESPONSE FACTOR 3 (SsARF3), are highly expressed in dorsal petals, suggesting that auxin regulation is involved in dorsi-ventral petal asymmetry. Here, the endogenous IAA level in dorsal petals is higher than ventral petals, as determined by UPLC/MS/MS. NPA (auxin inhibitor) treatment affected petal growth by reducing the length of dorsal corolla tube. The positive regulation between SsCYC and SsARF3 was examined by a dual luciferase assay. Phenotypic analysis in Arabidopsis transgenic plants inferred that SsARF3 was a repressor, as was the SsARF3-SRDX fusion protein, which functions to promote petal cell expansion and reduce primary inflorescence. These results suggest that auxin and SsARF3 may coordinate with SsCYC for establishment of dorsi-ventral petal asymmetry. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-08-15T17:30:07Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-08-15T17:30:07Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 謝誌 I
摘要 II Abstract III Content IV Index of Figures VII Index of Tables VIII Index of Supplementary data IX Abbreviations XI Introduction 1 1.1 CYCLOEDIA governs floral symmetry in Sinningia speciosa 1 1.1.1 Regulation of CYC-RAD-DIV from Antirrhinum to Sinningia 1 1.1.2 Ten-base deletion in SsCYC makes zygomorphic flower turn into actinomorphic flower (peloric) in Ss‘ES’ 2 1.2 The dorsi-ventral asymmetric cell growth shaped the petals in Ss‘ES’ 3 1.2.1 Inner epidermis cell size of dorsal petal was larger than that of ventral petals 3 1.2.2 Auxin signaling pathway is distinguishable in the dorsal petals in FB5 by transcriptomic analysis 6 1.3 Aim of study 10 Materials and Methods 11 2.1 Plant materials and growth condition 11 2.2 Total RNA extraction and reverse transcription 12 2.3 Endogenous IAA determination 13 2.4 Flower auxin inhibitor treatment 14 2.5 Genomic DNA extraction 15 2.6 Isolation of the 5’ regulatory region of Ss‘ES’ 16 2.7 Prediction of the promoter binding site 18 2.8 Vector construction for Arabidopsis transformants 18 2.9 Site directed mutagenesis 20 2.10 Floral dip transformation 22 2.11 Arabidopsis transgenic plants selection 23 2.12 Petal analysis 23 2.13 Auxin sensitivity root assay 25 2.14 Vector construction for dual-luciferase assay 26 2.15 Agrobacterium tumefaciens competent cell preparation and transformation 29 2.16 Dual-luciferase assay 30 2.17 Polymerase chain reaction 34 2.18 Real-time polymerase chain reaction 35 2.19 Statistical analyses 36 Results 37 3.1 Endogenous IAA level is higher in dorsal petals in FB5 37 3.2 Auxin inhibitors restrict dorsal corolla tube growth in Ss‘ES’ 40 3.3 The expression of SsARF3 is regulated by SsCYC 42 3.4 Ectopic expression of SsARF3 increase petal area by enhancing cell expansion 46 Discussions 58 4.1 Auxin gradient is established for the dorsal corolla tube growth in FB5 58 4.2 The SsARF3 gene participates in a different gene regulatory network in proximal petals 60 4.3 The SsARF3 gene and SsCYC gene might share gene regulatory network 62 Conclusion 68 References 70 Supplementary data 88 | - |
| dc.language.iso | en | - |
| dc.title | 生長素及生長素響應因子SsARF3 對大岩桐背腹花瓣建立的影響 | zh_TW |
| dc.title | The effect of Auxin and AUXIN RESPONSE FACTOR3 for establishment of dorsi-ventral petal asymmetry in Sinningia speciosa | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 鄭貽生;陳仁治;鄭惠國 | zh_TW |
| dc.contributor.oralexamcommittee | Yi-Sheng Cheng;Jen-Chih Chen;Ooi-Kock Teh | en |
| dc.subject.keyword | 大岩桐屬,CYCLOIDEA,兩側對稱,背腹花瓣不對稱,生長素,生長素響應因子ARF3, | zh_TW |
| dc.subject.keyword | Sinningia,CYCLOIDEA,zygomorphy,dorsi-ventral petal asymmetry,auxin,AUXIN RESPONSE FACTOR 3, | en |
| dc.relation.page | 107 | - |
| dc.identifier.doi | 10.6342/NTU202302660 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2023-08-08 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 生命科學系 | - |
| 顯示於系所單位： | 生命科學系 | |
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