請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/87672完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃榮南 | zh_TW |
| dc.contributor.advisor | Rong-Nan Huang | en |
| dc.contributor.author | 林宥孜 | zh_TW |
| dc.contributor.author | Yu-Tzu Lin | en |
| dc.date.accessioned | 2023-07-11T16:14:52Z | - |
| dc.date.available | 2024-06-30 | - |
| dc.date.copyright | 2023-07-11 | - |
| dc.date.issued | 2022 | - |
| dc.date.submitted | 2002-01-01 | - |
| dc.identifier.citation | 衛生福利部疾病管制署, 2021。登革熱/屈公病防治工作指引。衛生福利部疾病管制署。5-10頁。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/87672 | - |
| dc.description.abstract | 蚊蟲是許多疾病的傳播媒介，包含瘧疾、登革熱和茲卡病毒感染症。蚊蟲的防治策略通常依賴合成殺蟲劑，但合成殺蟲劑常產生抗藥性，也有環境污染問題，因此，必須尋找嶄新的防治策略，而精油是一種環境友善防治害蟲很好的替代方案。實驗室先前研究顯示相較於埃及斑蚊幼蟲，熱帶家蚊幼蟲對左手香精油較敏感，而β-石竹烯有可能是造成左手香精油對埃及斑蚊及熱帶家蚊幼蟲毒性不同的主要成分。本研究的目的為探討左手香精油對埃及斑蚊及熱帶家蚊幼蟲毒性差異機制。細胞色素P450 (CYP) 及穀胱甘肽S-轉移酶 (GST) 在蚊蟲對抗殺蟲劑的過程中扮演重要角色，實驗結果顯示埃及斑蚊幼蟲GSTe2及CYP6M family的表現量均較熱帶家蚊幼蟲高，尤其GSTe2的表現量於埃及斑蚊幼蟲更顯著高於於熱帶家蚊幼蟲。胡椒基丁醚 (細胞色素P450抑制劑) 及1-氟-2,4-二硝基苯 (GSH抑制劑) 均可提升埃及斑蚊及熱帶家蚊幼蟲對β-石竹烯的敏感性，但以1-氟-2,4-二硝基苯對埃及斑蚊幼蟲的影響較對熱帶家蚊幼蟲顯著。顯示GSH的含量與埃及斑蚊及熱帶家蚊幼蟲對β-石竹烯的敏感度有關，為造成左手香精油對埃及斑蚊及熱帶家蚊幼蟲毒性差異的主要原因。 | zh_TW |
| dc.description.abstract | Mosquitoes are vectors for many diseases, including malaria, dengue fever, and Zika virus infection. Strategies for mosquito control mainly rely on synthetic insecticides which always results in insecticide resistance and environmental pollution. Therefore, development of eco-friendly strategies for vector control is urgent and essential oil (EO) is an alternative method for pest control. Previous studies have shown larva of Culex quinquefasciatus was more sensitive than that of Aedes aegypti to Plectranthus amboinicus EOs. β-caryophyllene might be the major component responsible for the differential toxicity of P. amboinicus EOs, as indicated by the significant differences in its LC50 values toward both mosquitoes. This thesis aims to delineate the mechanisms responsible for the differential toxicity of P. amboinicus EOs toward both mosquito larvae. The currrent results showed that the relative expression levels of GSTe2 (as well as GSH contents) and CYP6M family in Ae. aegypti larvae were higher than that in Cx. quinquefasciatus larvae. In particular, the difference of GSTe2 is more significant than that of CYP6M family in both mosquito larvae. Though both piperonyl butoxide (PBO, an inhibitor of cytochrome P450) and 1-fluoro-2,4-dinitrobenzene (DNFB, a GSH-depleting agents) treatment potentiated the sensitivity of Ae. aegypti and Cx. quinquefasciatus larvae to β-caryophyllene, DNFB imposed more significant effect in Ae. aegypti larvae than to Cx. quinquefasciatus larvae. These results suggested both GSH contents and GSTs level are the major factors contributing to the different sensitivity of Ae. aegypti and Cx. quinquefasciatus to β-caryophyllene and the mechanism responsible for the differential toxicity of P. amboinicus essential oils toward Aedes and Culex mosquito larvae. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-07-11T16:14:52Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-07-11T16:14:52Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 目 錄
論文口試委員會審定書…………………………………………………………………i 摘要……………………………………………………………………………………...ii Abstract……………………………………………………………………………........iii 目錄……………………………………………………………………………………...v 圖目錄…………………………………………………………………………………...x 壹、 緒言………………………………………………………………………………...1 貳、 材料與方法……………………………………………………………………….13 2.1 試驗昆蟲飼養…………………………………………………………………..13 2.1.1 埃及斑蚊及熱帶家蚊飼養………………………………………………...13 2.1.2蚊蟲餵血……………………………………………………………….……13 2.2 β-石竹烯處理埃及斑蚊及熱帶家蚊幼蟲………………………………………14 2.3 埃及斑蚊與熱帶家蚊CYP、GST及Cyt C Oxidase基因表現………………....14 2.3.1 埃及斑蚊與熱帶家蚊幼蟲RNA萃取…………………………….……….14 2.3.2 反轉錄RNA (Reverse transcription, RT) 至cDNA………………………..15 2.3.3 聚合酶連鎖反應 (Polymerase chain reaction, PCR)………………………15 2.3.4 PCR產物分析分析…………………………………………………………16 2.4 固定抑制劑濃度處理不同濃度β-石竹烯實驗…………………………..…….16 2.5 改變抑制劑濃度處理50 ppm β-石竹烯濃度實驗……………………………..17 2.6 埃及斑蚊及熱帶家蚊幼蟲GSH含量分析………………………….…………18 2.6.1 DNFB對兩種幼蟲GSH含量影響試驗……………………….…………...18 2.6.2 DNFB及β-石竹烯對幼蟲GSH含量影響試驗……………………………18 2.6.3 蛋白質定量………………………………………………………………...18 2.6.4 GSH含量測定…………………………………..………………………......19 2.7 數據分析………………………………………………………………………...19 參、 結果……………………………………………………………………………….20 3.1 β-石竹烯對埃及斑蚊及熱帶家蚊幼蟲毒性……………………………………20 3.2 埃及斑蚊及熱帶家蚊CYP 6M、GSTe2及Cyt C Oxidase基因表現……..……20 3.3 β-石竹烯與抑制劑對幼蟲毒性之影響…………………………………...…….20 3.3.1 同時處理β-石竹烯與PBO對埃及斑蚊幼蟲毒性之影響………………...21 3.3.2 同時處理β-石竹烯與PBO對熱帶家蚊幼蟲毒性之影響………………...21 3.3.3同時處理β-石竹烯與PBO對埃及斑蚊與熱帶家蚊幼蟲毒性提升幅度………………………………………………………………………………….21 3.3.4 同時處理β-石竹烯與DEM對埃及斑蚊幼蟲毒性之影響………………..22 3.3.5 同時處理β-石竹烯與DEM對熱帶家蚊幼蟲毒性之影響………………..22 3.3.6 同時處理β-石竹烯與DNFB對埃及斑蚊幼蟲毒性之影響…………...….22 3.3.7 同時處理β-石竹烯與DNFB對熱帶家蚊幼蟲毒性之影響……….…..….23 3.3.8 同時處理β-石竹烯與DNFB埃及斑蚊及熱帶家蚊幼蟲毒性提升幅度………………………………………………………………………………….23 3.4 抑制劑前處理對β-石竹烯殺幼蟲毒性之影響………………………………..23 3.4.1 PBO前處理對β-石竹烯毒殺埃及斑蚊幼蟲毒性之影響…………….……23 3.4.2 PBO前處理對β-石竹烯毒殺熱帶家蚊幼蟲毒性之影響……………….…24 3.4.3 PBO前處理影響β-石竹烯對埃及斑蚊及熱帶家蚊幼蟲死亡率提升幅度………………………………………………………………………………….24 3.4.4 DEM前處理對β-石竹烯毒殺埃及斑蚊幼蟲毒性之影響………………...25 3.4.5 DEM前處理對 β-石竹烯毒殺熱帶家蚊幼蟲毒性之影響……………….25 3.4.6 DNFB前處理對β-石竹烯毒殺埃及斑蚊幼蟲毒性之影響……………….25 3.4.7 DNFB前處理對β-石竹烯毒殺熱帶家蚊幼蟲毒性之影響……………….25 3.4.8 DNFB前處理影響β-石竹烯對埃及斑蚊及熱帶家蚊幼蟲死亡率提升幅度………………………………………………………………………………….26 3.5 抑制劑濃度對β-石竹烯毒性之影響……………………………………….…..26 3.5.1 同時處理不同濃度PBO對β-石竹烯毒殺埃及斑蚊幼蟲之影響………...26 3.5.2 同時處理不同濃度PBO對β-石竹烯毒殺熱帶家蚊幼蟲之影響………...26 3.5.3 同時處理不同濃度DEM對β-石竹烯毒殺埃及斑蚊幼蟲之影響……….27 3.5.4 同時處理不同濃度DEM對β-石竹烯毒殺熱帶家蚊幼蟲之影響……….27 3.5.5 同時處理不同濃度DNFB對β-石竹烯毒殺埃及斑蚊幼蟲之影響………………………………………………………………………………….27 3.5.6 同時處理不同濃度DNFB對β-石竹烯毒殺熱帶家蚊幼蟲之影響………………………………………………………………………………….27 3.5.7 同時處理不同濃度DNFB提升β-石竹烯毒性倍率比較……………...….28 3.6 不同濃度抑制劑前處理對β-石竹烯毒性影響…………………………….…..28 3.6.1不同濃度PBO前處理影響β-石竹烯對埃及斑蚊幼蟲毒性………………28 3.6.2不同濃度PBO前處理影響β-石竹烯對熱帶家蚊幼蟲毒性………………28 3.6.3不同濃度DEM前處理影響β-石竹烯對埃及斑蚊幼蟲毒性……………...29 3.6.4不同濃度DEM前處理影響β-石竹烯對熱帶家蚊幼蟲毒性……………...29 3.6.5不同濃度DNFB前處理影響β-石竹烯對埃及斑蚊幼蟲毒性……………..29 3.6.6不同濃度DNFB前處理影響β-石竹烯對熱帶家蚊幼蟲毒性………….….30 3.6.7 提升DNFB濃度影響β-石竹烯對埃及斑蚊及熱帶家蚊毒性提升倍率比較………………………………………………………………………………….30 3.7 DNFB與β-石竹烯對蚊幼蟲GSH含量影響……………………………………30 3.7.1 DNFB對埃及斑蚊及熱帶家蚊GSH含量影響……………………..……..31 3.7.2 DNFB處理埃及斑蚊及熱帶家蚊幼蟲GSH含量下降幅度比較……..…..31 3.7.3 同時處理DNFB及β-石竹烯對埃及斑蚊及熱帶家蚊GSH含量影響………………………………………………………………………………….31 3.7.4 DNFB及β-石竹烯對埃及斑蚊及熱帶家蚊GSH含量下降幅度比較………………………………………………………………………………….32 肆、 討論……………………………………………………………………………….33 伍、 參考文獻………………………………………………………………………….38 陸、 附錄……………………………………………………………………………….73 附錄一、本研究使用之引子清單………………………………………………….73 附錄二、PBO同時處理埃及斑蚊及熱帶家蚊幼蟲6小時對β-石竹烯毒性提升倍 率…………………………………………………………………………………….74 附錄三、PBO同時處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍 率………………………………………………………………………………….…75 附錄四、PBO前處理埃及斑蚊及熱帶家蚊幼蟲6小時對β-石竹烯毒性提升倍率…………………………………………………………………………………….76 附錄五、PBO前處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率…………………………………………………………………………………….77 附錄六、DNFB同時處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率………………………………………………………………………………….78 附錄七、DNFB同時處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率………………………………………………………………………………….79 附錄八、DNFB前處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率…………………………………………………………………………………….80 圖目錄 圖一、β-石竹烯對埃及斑蚊及熱帶家蚊幼蟲的毒性………………………….……...44 圖二、埃及斑蚊及熱帶家蚊CYP 6M家族基因表現比較……………………………45 圖三、埃及斑蚊及熱帶家蚊GSTe2基因表現比較………………………………..….46 圖四、埃及斑蚊及熱帶家蚊Cyt C Oxidase基因表現比較………………………….47 圖五、PBO與β-石竹烯同時處理對埃及斑蚊及熱帶家蚊幼蟲毒性的影響……………………………………………………………………………………….48 圖六、PBO同時處理埃及斑蚊及熱帶家蚊幼蟲6小時對β-石竹烯毒性提升倍率……………………………………………………………………………………….49 圖七、PBO同時處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率……………………………………………………………………………………….50 圖八、DEM與β-石竹烯同時處理對埃及斑蚊及熱帶家蚊幼蟲毒性的影響…………………………………………………………………………………….…51 圖九、DNFB與β-石竹烯同時處理對埃及斑蚊及熱帶家蚊幼蟲毒性的影響……………………………………………………………………………………….52 圖十、DNFB同時處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率……………………………………………………………………………………….53 圖十一、DNFB同時處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率………………………………………………………………………………...……..54 圖十二、PBO前處理影響β-石竹烯對埃及斑蚊及熱帶家蚊幼蟲的毒性………………………………………………………….……………………………55 圖十三、PBO前處理埃及斑蚊及熱帶家蚊幼蟲6小時對β-石竹烯毒性提升倍率……………………………………………………………………………………….56 圖十四、PBO前處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率……………………………………………………………………………………….57 圖十五、DEM前處理影響β-石竹烯對埃及斑蚊及熱帶家蚊幼蟲的毒性……………………………………………………………………………………….58 圖十六、DNFB前處理影響β-石竹烯對埃及斑蚊及熱帶家蚊幼蟲的毒性…………………………………………………………………………….…………59 圖十七、DNFB前處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率………………………………………………………………………………...……..60 圖十八、不同濃度PBO同時處理埃及斑蚊及熱帶家蚊幼蟲對β-石竹烯毒性的影響…………………………………………………………………….…………………61 圖十九、不同濃度DEM同時處理埃及斑蚊及熱帶家蚊幼蟲對β-石竹烯毒性的影響…………………………………………………………………………………….....62 圖二十、不同濃度DNFB同時處理埃及斑蚊及熱帶家蚊幼蟲對β-石竹烯毒性的影響……………………………………………………………………………………….63 圖二十一、不同濃度DNFB同時處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率…………………………………………………………....…………….64 圖二十二、不同濃度PBO前處理埃及斑蚊及熱帶家蚊幼蟲對β-石竹烯毒性的影響……………………………………………………………………………………….65 圖二十三、不同濃度DEM前處理埃及斑蚊及熱帶家蚊幼蟲對β-石竹烯毒性的影響……………………………………………………………………………………….66 圖二十四、不同濃度DNFB前處理埃及斑蚊及熱帶家蚊幼蟲對β-石竹烯毒性的影響……………………………………………………………………………………….67 圖二十五、不同濃度DNFB前處理埃及斑蚊及熱帶家蚊幼蟲12小時對β-石竹烯毒性提升倍率………………………………………………………………….…………68 圖二十六、DNFB對埃及斑蚊及熱帶家蚊幼蟲GSH含量影響……………………...69 圖二十七、DNFB處理埃及斑蚊及熱帶家蚊幼蟲GSH含量下降幅度……………...70 圖二十八、DNFB及β-石竹烯對埃及斑蚊及熱帶家蚊幼蟲GSH含量影響…………………………………………………………………………………….…71 圖二十九、DNFB及β-石竹烯處理埃及斑蚊及熱帶家蚊幼蟲GSH含量下降幅度………………………………………………………………………….……………72 | - |
| dc.language.iso | zh_TW | - |
| dc.title | 左手香精油對埃及斑蚊及熱帶家蚊幼蟲差異毒性機制研究 | zh_TW |
| dc.title | The study of mechanism for the differential toxicity of Plectranthus amboinicus essential oils toward Aedes aegypti and Culex quinquefasciatus larvae | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 110-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 黃旌集 | zh_TW |
| dc.contributor.oralexamcommittee | Kun-Hsien Tsai;Ju-Chun Hsu;Chin-Gi Huang | en |
| dc.subject.keyword | 埃及斑蚊,熱帶家蚊,左手香精油,β-石竹烯,細胞色素P450,穀胱甘肽S-轉移酶, | zh_TW |
| dc.subject.keyword | Aedes aegypti,Culex quinquefasciatus,Plectranthus amboinicus essential oils,β-caryophyllene,Cytochrome P450,Glutathione-S-transferase, | en |
| dc.relation.page | 80 | - |
| dc.identifier.doi | 10.6342/NTU202200821 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2022-05-31 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 昆蟲學系 | - |
| dc.date.embargo-lift | 2024-06-30 | - |
| 顯示於系所單位： | 昆蟲學系 | |
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