請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/86338完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳慧文(Hui-Wen Chen) | |
| dc.contributor.author | Yao-Yun Chen | en |
| dc.contributor.author | 陳耀云 | zh_TW |
| dc.date.accessioned | 2023-03-19T23:49:57Z | - |
| dc.date.copyright | 2022-08-26 | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2022-08-25 | |
| dc.identifier.citation | Archetti, I., Horsfall, F.L., Jr., 1950. Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum. J Exp Med 92, 441-462. Azkur, A.K., Akdis, M., Azkur, D., Sokolowska, M., van de Veen, W., Brüggen, M.C., O’Mahony, L., Gao, Y., Nadeau, K., Akdis, C.A., 2020. Immune response to SARS‐CoV‐2 and mechanisms of immunopathological changes in COVID‐19. Allergy 75, 1564-1581. Bande, F., Arshad, S.S., Omar, A.R., Hair-Bejo, M., Mahmuda, A., Nair, V., 2017. Global distributions and strain diversity of avian infectious bronchitis virus: a review. Animal health research reviews 18, 70-83. Boley, P.A., Alhamo, M.A., Lossie, G., Yadav, K.K., Vasquez-Lee, M., Saif, L.J., Kenney, S.P., 2020. Porcine deltacoronavirus infection and transmission in poultry, United States. Emerging infectious diseases 26, 255. Buzdugan, I., Trifan, M., Savuta, G., 2021. The epidemioloy and zoonotic risks of coronaviruses. Scientific papers veterinary series 64, 10. Cavanagh, D., 2007. Coronavirus avian infectious bronchitis virus. Veterinary research 38, 281-297. Cawood, R., Harrison, S.M., Dove, B.K., Reed, M.L., Hiscox, J.A., 2007. Cell cycle dependent nucleolar localization of the coronavirus nucleocapsid protein. Cell Cycle 6, 863-867. Chan, K., Cheng, V., Woo, P., Lau, S., Poon, L., Guan, Y., Seto, W., Yuen, K., Peiris, J., 2005. Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63. Clinical and vaccine immunology 12, 1317-1321. Che, X.Y., Hao, W., Wang, Y., Di, B., Yin, K., Xu, Y.C., Feng, C.S., Wan, Z.Y., Cheng, V.C., Yuen, K.Y., 2004. Nucleocapsid protein as early diagnostic marker for SARS. Emerg Infect Dis 10, 1947-1949. Chowdhury, M.A., Hossain, N., Kashem, M.A., Shahid, M.A., Alam, A., 2020. Immune response in COVID-19: A review. Journal of infection and public health 13, 1619-1629. Cubuk, J., Alston, J.J., Incicco, J.J., Singh, S., Stuchell-Brereton, M.D., Ward, M.D., Zimmerman, M.I., Vithani, N., Griffith, D., Wagoner, J.A., 2021. The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA. Nature communications 12, 1-17. de Groot, R.J., Baker, S., Baric, R., Enjuanes, L., Gorbalenya, A., Holmes, K., Perlman, S., Poon, L., Rottier, P., Talbot, P. 2011. Family coronaviridae, In: Virus taxonomy. Duan, C., 2021. An updated review of porcine deltacoronavirus in terms of prevalence, pathogenicity, pathogenesis and antiviral strategy. Frontiers in veterinary science 8. Edridge, A.W., Kaczorowska, J., Hoste, A.C., Bakker, M., Klein, M., Loens, K., Jebbink, M.F., Matser, A., Kinsella, C.M., Rueda, P., 2020. Seasonal coronavirus protective immunity is short-lasting. Nature medicine 26, 1691-1693. Eguia, R.T., Crawford, K.H., Stevens-Ayers, T., Kelnhofer-Millevolte, L., Greninger, A.L., Englund, J.A., Boeckh, M.J., Bloom, J.D., 2021. A human coronavirus evolves antigenically to escape antibody immunity. PLoS pathogens 17, e1009453. Felsenstein, J., 1985. CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP. Evolution 39, 783-791. Fung, T.S., Liu, D.X., 2019. Human coronavirus: host-pathogen interaction. Annual review of microbiology 73, 529-557. Gao, T., Gao, Y., Liu, X., Nie, Z., Sun, H., Lin, K., Peng, H., Wang, S., 2021. Identification and functional analysis of the SARS-COV-2 nucleocapsid protein. BMC Microbiol 21, 58. García-Salido, A., 2020. Narrative review of the immune response against coronavirus: An overview, applicability for SARS-COV-2, and therapeutic implications. Anales de pediatría (english edition) 93, 60. e61-60. e67. Gimenez-Lirola, L.G., Zhang, J., Carrillo-Avila, J.A., Chen, Q., Magtoto, R., Poonsuk, K., Baum, D.H., Piñeyro, P., Zimmerman, J., 2017. Reactivity of porcine epidemic diarrhea virus structural proteins to antibodies against porcine enteric coronaviruses: diagnostic implications. Journal of clinical microbiology 55, 1426-1436. Grabherr, S., Ludewig, B., Pikor, N.B., 2021. Insights into coronavirus immunity taught by the murine coronavirus. European journal of immunology 51, 1062-1070. Guo, L., Ren, L., Yang, S., Xiao, M., Chang, D., Yang, F., Dela Cruz, C.S., Wang, Y., Wu, C., Xiao, Y., Zhang, L., Han, L., Dang, S., Xu, Y., Yang, Q.W., Xu, S.Y., Zhu, H.D., Xu, Y.C., Jin, Q., Sharma, L., Wang, L., Wang, J., 2020. Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19). Clin Infect Dis 71, 778-785. Halstead, S.B., Katzelnick, L., 2020. COVID-19 vaccines: should we fear ADE? The journal of infectious diseases 222, 1946-1950. Hartmann, K., 2005. Feline infectious peritonitis. Veterinary clinics: small animal practice 35, 39-79. He, Q., Du, Q., Lau, S., Manopo, I., Lu, L., Chan, S.-W., Fenner, B.J., Kwang, J., 2005. Characterization of monoclonal antibody against SARS coronavirus nucleocapsid antigen and development of an antigen capture ELISA. Journal of virological methods 127, 46-53. Herrewegh, A.A., Smeenk, I., Horzinek, M.C., Rottier, P.J., De Groot, R.J., 1998. Feline coronavirus type II strains 79-1683 and 79-1146 originate from a double recombination between feline coronavirus type I and canine coronavirus. Journal of virology 72, 4508-4514. Hsu, T.H., Liu, H.P., Chin, C.Y., Wang, C., Zhu, W.Z., Wu, B.L., Chang, Y.C., 2018. Detection, sequence analysis, and antibody prevalence of porcine deltacoronavirus in Taiwan. Archives of virology 163, 3113-3117. Hu, B., Guo, H., Zhou, P., Shi, Z.-L., 2021. Characteristics of SARS-CoV-2 and COVID-19. Nature reviews microbiology 19, 141-154. Hu, H., Jung, K., Vlasova, A.N., Chepngeno, J., Lu, Z., Wang, Q., Saif, L.J., 2015. Isolation and characterization of porcine deltacoronavirus from pigs with diarrhea in the United States. Journal of clinical microbiology 53, 1537-1548. Jemeršić, L., Lojkić, I., Krešić, N., Keros, T., Zelenika, T.A., Jurinović, L., Skok, D., Bata, I., Boras, J., Habrun, B., 2021. Investigating the presence of SARS CoV-2 in free-living and captive animals. Pathogens 10, 635. Jia, W., Naqi, S.A., 1997. Sequence analysis of gene 3, gene 4 and gene 5 of avian infectious bronchitis virus strain CU-T2. Gene 189, 189-193. Kang, S., Yang, M., Hong, Z., Zhang, L., Huang, Z., Chen, X., He, S., Zhou, Z., Zhou, Z., Chen, Q., Yan, Y., Zhang, C., Shan, H., Chen, S., 2020. Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites. Acta Pharm Sin B 10, 1228-1238. Kim, H., Seiler, P., Jones, J.C., Ridout, G., Camp, K.P., Fabrizio, T.P., Jeevan, T., Miller, L.A., Throm, R.E., Ferrara, F., 2020a. Antibody responses to SARS-CoV-2 antigens in humans and animals. Vaccines 8, 684. Kim, Y.-I., Kim, S.-G., Kim, S.-M., Kim, E.-H., Park, S.-J., Yu, K.-M., Chang, J.-H., Kim, E.J., Lee, S., Casel, M.A.B., 2020b. Infection and rapid transmission of SARS-CoV-2 in ferrets. Cell host & microbe 27, 704-709. e702. Klompus, S., Leviatan, S., Vogl, T., Mazor, R.D., Kalka, I.N., Stoler-Barak, L., Nathan, N., Peres, A., Moss, L., Godneva, A., 2021. Cross-reactive antibodies against human coronaviruses and the animal coronavirome suggest diagnostics for future zoonotic spillovers. Science immunology 6, eabe9950. Kolaskar, A.S., Tongaonkar, P.C., 1990. A semi-empirical method for prediction of antigenic determinants on protein antigens. FEBS Lett 276, 172-174. Lednicky, J.A., Tagliamonte, M.S., White, S.K., Elbadry, M.A., Alam, M., Stephenson, C.J., Bonny, T.S., Loeb, J.C., Telisma, T., Chavannes, S., 2021. Independent infections of porcine deltacoronavirus among Haitian children. Nature 600, 133-137. Lee, H.-K., Lee, B.-H., Seok, S.-H., Baek, M.-W., Lee, H.-Y., Kim, D.-J., Na, Y.-R., Noh, K.-J., Park, S.-H., Kumar, D.N., 2010. Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43. Journal of veterinary science 11, 165-167. Lee, H.K., Lee, B.H., Dutta, N.K., Seok, S.H., Baek, M.W., Lee, H.Y., Kim, D.J., Na, Y.R., Noh, K.J., Park, S.H., 2008. Detection of antibodies against SARS-Coronavirus using recombinant truncated nucleocapsid proteins by ELISA. Journal of microbiology and biotechnology. Li, B., Ge, J., Li, Y., 2007. Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus. Virology 365, 166-172. Li, F., 2015. Receptor recognition mechanisms of coronaviruses: a decade of structural studies. Journal of virology 89, 1954-1964. Li, W., Hulswit, R.J.G., Kenney, S.P., Widjaja, I., Jung, K., Alhamo, M.A., Dieren, B.v., Kuppeveld, F.J.M.v., Saif, L.J., Bosch, B.-J., 2018. Broad receptor engagement of an emerging global coronavirus may potentiate its diverse cross-species transmissibility. Proceedings of the national academy of sciences 115, E5135-E5143. Lin, C.-Y., Wolf, J., Brice, D.C., Sun, Y., Locke, M., Cherry, S., Castellaw, A.H., Wehenkel, M., Crawford, J.C., Zarnitsyna, V.I., 2022. Pre-existing humoral immunity to human common cold coronaviruses negatively impacts the protective SARS-CoV-2 antibody response. Cell host & microbe 30, 83-96. e84. Lin, S.-Y., Li, Y.-T., Chen, Y.-T., Chen, T.-C., Hu, C.-M.J., Chen, H.-W., 2016. Identification of an infectious bronchitis coronavirus strain exhibiting a classical genotype but altered antigenicity, pathogenicity, and innate immunity profile. Scientific reports 6, 1-13. Liu, X., Verma, A., Garcia, G., Jr., Ramage, H., Lucas, A., Myers, R.L., Michaelson, J.J., Coryell, W., Kumar, A., Charney, A.W., Kazanietz, M.G., Rader, D.J., Ritchie, M.D., Berrettini, W.H., Schultz, D.C., Cherry, S., Damoiseaux, R., Arumugaswami, V., Klein, P.S., 2021. Targeting the coronavirus nucleocapsid protein through GSK-3 inhibition. Proc Natl Acad Sci U S A 118. Luo, Y.-C., Liu, I.-L., Chen, Y.-T., Chen, H.-W., 2020. Detection of feline coronavirus in feline effusions by immunofluorescence staining and reverse transcription polymerase chain reaction. Pathogens 9, 698. Ma, Y., Zhang, Y., Liang, X., Oglesbee, M., Krakowka, S., Niehaus, A., Wang, G., Jia, A., Song, H., Li, J., 2016. Two-way antigenic cross-reactivity between porcine epidemic diarrhea virus and porcine deltacoronavirus. Vet microbiol 186, 90-96. Malone, B., Urakova, N., Snijder, E.J., Campbell, E.A., 2022. Structures and functions of coronavirus replication–transcription complexes and their relevance for SARS-CoV-2 drug design. Nature reviews molecular cell biology 23, 21-39. McBride, R., van Zyl, M., Fielding, B.C., 2014. The coronavirus nucleocapsid is a multifunctional protein. Viruses 6, 2991-3018. McIntosh, K., Hirsch, M., Bloom, A., 2021. COVID-19: Epidemiology, virology, and prevention. Uptodate. 18. Olsen, C.W., Corapi, W., Ngichabe, C., Baines, J., Scott, F., 1992. Monoclonal antibodies to the spike protein of feline infectious peritonitis virus mediate antibody-dependent enhancement of infection of feline macrophages. Journal of virology 66, 956-965. Paltrinieri, S., Giordano, A., Stranieri, A., Lauzi, S., 2021. Feline infectious peritonitis (FIP) and coronavirus disease 19 (COVID‐19): Are they similar? Transboundary and emerging diseases 68, 1786-1799. Pedersen, N.C., 2009. A review of feline infectious peritonitis virus infection: 1963–2008. Journal of feline medicine and surgery 11, 225-258. Perlman, S., 2020. Another decade, another coronavirus. The new england journal of medicine 382, 760-762. Quinlan, B.D., Mou, H., Zhang, L., Guo, Y., He, W., Ojha, A., Parcells, M.S., Luo, G., Li, W., Zhong, G., 2020. The SARS-CoV-2 receptor-binding domain elicits a potent neutralizing response without antibody-dependent enhancement. Biorxiv preprint Rai, P., Kumar, B.K., Deekshit, V.K., Karunasagar, I., Karunasagar, I., 2021. Detection technologies and recent developments in the diagnosis of COVID-19 infection. Applied microbiology and biotechnology 105, 441-455. Saitou, N., Nei, M., 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406-425. Sariol, A., Perlman, S., 2020. Lessons for COVID-19 immunity from other coronavirus infections. Immunity 53, 248-263. Stecher, G., Tamura, K., Kumar, S., 2020. Molecular Evolutionary Genetics Analysis (MEGA) for macOS. Molecular Biology and Evolution 37, 1237-1239. Stout, A.E., André, N.M., Jaimes, J.A., Millet, J.K., Whittaker, G.R., 2020. Coronaviruses in cats and other companion animals: Where does SARS-CoV-2/COVID-19 fit? Veterinary microbiology 247, 108777. Su, M., Chen, Y., Qi, S., Shi, D., Feng, L., Sun, D., 2020. A mini-review on cell cycle regulation of coronavirus infection. Frontiers in veterinary science 7, 586826. Su, M., Li, C., Guo, D., Wei, S., Wang, X., Geng, Y., Yao, S., Gao, J., Wang, E., Zhao, X., 2015. A recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus. Journal of veterinary medical science, 15-0533. Sun, Z., Meng, X., 2004. Antigenic cross-reactivity between the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus and polyclonal antisera of antigenic group I animal coronaviruses: implication for SARS diagnosis. Journal of clinical microbiology 42, 2351-2352. Tamura, K., Nei, M., Kumar, S., 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A 101, 11030-11035. Tang, Y.-W., Schmitz, J.E., Persing, D.H., Stratton, C.W., 2020. Laboratory diagnosis of COVID-19: current issues and challenges. Journal of clinical microbiology 58, e00512-00520. Tresnan, D.B., Levis, R., Holmes, K.V., 1996. Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. Journal of virology 70, 8669-8674. V’kovski, P., Kratzel, A., Steiner, S., Stalder, H., Thiel, V., 2021. Coronavirus biology and replication: implications for SARS-CoV-2. Nature reviews microbiology 19, 155-170. Valastro, V., Holmes, E.C., Britton, P., Fusaro, A., Jackwood, M.W., Cattoli, G., Monne, I., 2016. S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification. Infection, genetics and evolution 39, 349-364. Vlasova, A.N., Zhang, X., Hasoksuz, M., Nagesha, H.S., Haynes, L.M., Fang, Y., Lu, S., Saif, L.J., 2007a. Two-way antigenic cross-reactivity between severe acute respiratory syndrome coronavirus (SARS-CoV) and group 1 animal CoVs is mediated through an antigenic site in the N-terminal region of the SARS-CoV nucleoprotein. Journal of virology 81, 13365-13377. Vlasova, A.N., Zhang, X., Hasoksuz, M., Nagesha, H.S., Haynes, L.M., Fang, Y., Lu, S., Saif, L.J., 2007b. Two-way antigenic cross-reactivity between severe acute respiratory syndrome coronavirus (SARS-CoV) and group 1 animal CoVs is mediated through an antigenic site in the N-terminal region of the SARS-CoV nucleoprotein. Journal of virology 81, 13365-13377. Wan, Y., Shang, J., Sun, S., Tai, W., Chen, J., Geng, Q., He, L., Chen, Y., Wu, J., Shi, Z., 2020. Molecular mechanism for antibody-dependent enhancement of coronavirus entry. Journal of virology 94, e02015-02019. Wu, D., Wu, T., Liu, Q., Yang, Z., 2020. The SARS-CoV-2 outbreak: what we know. International journal of infectious diseases 94, 44-48. Yamaoka, Y., Jeremiah, S.S., Miyakawa, K., Saji, R., Nishii, M., Takeuchi, I., Ryo, A., 2021. Whole nucleocapsid protein of severe acute respiratory syndrome coronavirus 2 may cause false-positive results in serological assays. Clinical infectious diseases 72, 1291-1292. Yang, Y.-L., Meng, F., Qin, P., Herrler, G., Huang, Y.-W., Tang, Y.-D., 2020. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerging microbes & infections 9, 457-468. Yen, S.-J., Chen, H.-W., 2021. Feline coronaviruses identified in feline effusions in suspected cases of feline infectious peritonitis. Microorganisms 9, 1801. Yu, F., Le, M.Q., Inoue, S., Hasebe, F., Parquet, M.d.C., Morikawa, S., Morita, K., 2007. Recombinant truncated nucleocapsid protein as antigen in a novel immunoglobulin M capture enzyme-linked immunosorbent assay for diagnosis of severe acute respiratory syndrome coronavirus infection. Clinical and vaccine immunology 14, 146-149. Yue, L., Cao, H., Xie, T., Long, R., Li, H., Yang, T., Yan, M., Xie, Z., 2021. N‐terminally truncated nucleocapsid protein of SARS‐CoV‐2 as a better serological marker than whole nucleocapsid protein in evaluating the immunogenicity of inactivated SARS‐CoV‐2. Journal of medical virology 93, 1732-1738. Zhang, J., 2016. Porcine deltacoronavirus: overview of infection dynamics, diagnostic methods, prevalence and genetic evolution. Virus research 226, 71-84. Zhang, Q., Zhang, H., Gao, J., Huang, K., Yang, Y., Hui, X., He, X., Li, C., Gong, W., Zhang, Y., 2020. A serological survey of SARS-CoV-2 in cat in Wuhan. Emerging microbes & infections 9, 2013-2019. Zhao, S., Li, W., Schuurman, N., Van Kuppeveld, F., Bosch, B.-J., Egberink, H., 2019. Serological screening for coronavirus infections in cats. Viruses 11, 743. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/86338 | - |
| dc.description.abstract | 冠狀病毒是一群有外套膜的單股正向 RNA 病毒，可感染人類和動物。冠狀病毒的核殼蛋白 (N) 是一種大量表現的多功能結構蛋白，對病毒的複製、組裝與釋放至關重要，N蛋白也具有高度免疫原性，因此常用於血清學診斷的目標抗原。然而，人與動物血清對不同冠狀病毒蛋白質是否存在著交叉反應，目前並不清楚。在這項研究中，我們旨在研究四種冠狀病毒 N蛋白之間的抗原交叉反應性。我們成功選殖並表現了group 1冠狀病毒貓冠狀病毒 (FCoV)、group 2冠狀病毒嚴重特殊傳染性肺炎冠狀病毒 (SARS-CoV-2)、group 3冠狀病毒傳染性支氣管炎病毒 (IBV)、和group 4冠狀病毒豬冠狀病毒 (PDCoV) 的重組N蛋白，同時使用小鼠製備這些重組 N蛋白的抗血清，另一方面，我們也分別收集了針對這些冠狀病毒感染呈現陽性或陰性的人類或動物臨床血清，在齊備各式抗原抗體之後，我們進行西方墨點法與酵素連結免疫吸附法進行交叉反應分析。結果顯示，我們在 FCoV與SARS-CoV-2 之臨床血清中觀察到雙向交叉反應，其中貓血清的FCoV N 蛋白抗體力價與 SARS-CoV-2 N 蛋白抗體力價之間存在顯著相關性；IBV與其他的冠狀病毒在N蛋白的抗原性未觀察到交叉反應，唯SARS-CoV-2 N 之兔抗血清能夠清楚辨識到 IBV 重組N 蛋白；另外，雖然我們在PDCoV N與 FCoV N 之老鼠抗血清之間觀察到雙向交叉反應，但因收集到PDCoV之臨床血清不論陰陽性對於PDCoV N蛋白結合力價皆不高，因此PDCoV與其他冠狀病毒之間的關係尚無法下定論；最後，我們以序列分析和蛋白質結構模擬呈現了四種冠狀病毒N蛋白的保守區段，顯示冠狀病毒之間可能發生交叉反應的抗原位點。藉由這項研究，我們指出人與動物的血清對於跨型別的冠狀病毒N蛋白存在著交叉反應，這些資訊可作為發展或進行冠狀病毒感染血清學診斷時的參考。 | zh_TW |
| dc.description.abstract | Coronavirus is a group of enveloped single-stranded positive-sense RNA viruses which infect humans and animals. The nucleocapsid (N) protein of coronavirus is a multifunctional structure protein that expresses abundantly and is essential for viral replication, assembly and release. N protein is frequently applied in serological assays for coronavirus detection due to its high immunogenicity. However, potential cross-reactivity among coronaviruses N proteins can complicate diagnosis and epidemiological analysis. In this study, we aim to investigate the antigenic cross-reactivity among four types of coronavirus N proteins. In this study, we have successfully cloned and expressed recombinant N proteins of feline coronavirus (FCoV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), infectious bronchitis virus (IBV) and porcine deltacoronavirus (PDCoV), which belong to alpha, beta, gamma, and delta coronavirus, respectively. Polyclonal antisera against these recombinant N proteins were prepared in mice. Meanwhile, positive or negative human/animal clinical sera against these coronaviruses were also collected. Western blot assays and ELISAs were performed to assess the antigenic cross-reactivity among coronavirus N proteins. Results showed that two-way cross-reactive responses were observed between the clinical sera of FCoV and SARS-CoV-2, and there exists a significant correlation between the FCoV N antibody titer and SARS-CoV-2 N antibody titer in feline clinical sera. No cross-reaction was found among IBV and other three coronaviruses, however, the SARS-CoV-2 N rabbit antiserum clearly detected IBV recombinant N protein. Although a two-way cross-reaction between mouse antiserum of PDCoV N and FCoV N was observed, the relationships among PDCoV and other coronaviruses remain inclusive as all the pig clinical sera showed a weak response with the PDCoV N protein. Furthermore, sequence analysis and protein structure prediction showed universal residues in conserved regions of the coronavirus N protein, highlighting potential common epitopes for anti-N antibody induction. Through this study, we address the antigenic cross-reactivity among coronavirus N proteins and provide essential information toward the development of serological diagnostics and surveillance for coronavirus infections. | en |
| dc.description.provenance | Made available in DSpace on 2023-03-19T23:49:57Z (GMT). No. of bitstreams: 1 U0001-2308202213224800.pdf: 14806107 bytes, checksum: 7a24ed808b94402e4e7f1a90f135aaf2 (MD5) Previous issue date: 2022 | en |
| dc.description.tableofcontents | Contents 中文摘要…………………………………………………………………………………I Abstract………………………………………………………………….……………..II List of figures…………………………………………………………...………..…...VII List of tables…………………………………………………...………………...…….IX Chapter 1 Introduction………………………………………...……………...………..1 1.1 Coronavirus .…………………………………...………………………...1 1.2 Coronavirus N protein……………………………………………………1 1.3 Replication of coronavirus……………………………………………….2 1.4 Host immune response to coronaviruses infection……………………….3 1.5 Diagnostic tools for coronavirus infection……………………………….5 1.6 Epidemiology of coronavirus………………………………….…………7 1.7 Feline coronavirus (FCoV) …………...………………………………….7 1.8 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …..…..9 1.9 Infectious bronchitis virus (IBV)…………..………………...…………10 1.10 Porcine deltacoronavirus (PDCoV)………….………………………….11 1.11 Cross-reactivity of N protein in human coronaviruses ..…………..…….12 1.12 Cross-reactivity of N protein in animal coronaviruses ..……..………….13 1.13 Study objectives………..……………………………………………….13 Chapter 2 Materials and Methods……………………………………………………15 2.1 Preparation of viral stocks and clinical serum………..…………………15 2.2 Virus isolation of PDCoV, next-generation sequencing (NGS), and sequence analysis………………………………………………………...……17 2.3 Amplification of coronaviruses N genes and SARS-CoV-2 N fragments.20 2.4 Construction of coronaviruses N genes and SARS-CoV-2 N fragments.21 2.5 Purification of coronaviruses N proteins.…………………….…………23 2.6 Bicinchoninic acid assay (BCA) ………………….……………………24 2.7 Preparation of hyperimmune mouse sera…………….……..…………..25 2.8 Validation of clinical sera…….…………………………………….…...26 2.9 Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot ……………………………………………………….………26 2.10 Enzyme-linked immunosorbent assay (ELISA) .…………….…….…...28 2.11 Cross-reaction assessments.……………………………………….…....29 2.12 Protein structure and sequence analysis.……………………………......29 2.13 Statistical analyses....………………………………….……………......30 Chapter 3 Results……………………………...………………………………………31 3.1 Isolation and characterization of PDCoV.……….……………...............31 3.2 Expression of FCoV N protein……………………………………….....31 3.3 Expression of SARS-CoV-2 N protein fragments…………..…………..32 3.4 Expression of IBV N protein……………………………………..…......33 3.5 Expression of PDCoV N protein……….……..…...................................34 3.6 Titration of mouse hyperimmune sera…………......................................35 3.7 Validation of clinical sera……..…...........................................................36 3.8 Cross-reaction assessments……………..................................................38 3.9 Analysis of potential cross-reactive motifs……………………………..42 3.10 Protein structure and sequence analysis………………………………...43 Chapter 4 Discussion………………………….……………………….………………46 Chapter 5 Figures and Tables...……………….…………………….…..……………52 Chapter 6 References………….……………………………...……….………..……103 List of figures Figure 1. Isolation and characterization of PDCoV NTU/C253/21…………………….52 Figure 2. FCoV N plasmid construction and protein expression. …………….……….55 Figure 3. SARS-CoV-2 N fragment plasmids construction and expression.…………..58 Figure 4. IBV N plasmid construction and protein expression. ……………………….61 Figure 5. PDCoV N plasmid construction and protein expression. …………………….64 Figure 6. Mouse hyperimmune serum preparation. ……………………………...…….67 Figure 7. FCoV clinical serum validation. ………………………………………….….69 Figure 8. SARS-CoV-2 clinical serum validation. ……………………………….…….71 Figure 9. IBV clinical serum validation. ………………………………...………….….73 Figure 10. PDCoV clinical serum validation. ……………………………….…………75 Figure 11. Cross-reaction assessment: FCoV N as the antigen for the WB and ELISA…………………………………………………………………………………..77 Figure 12. Cross-reaction assessment: SARS-CoV-2 N as the antigen for the WB and ELISA………………………………………………………….………………….……79 Figure 13. Cross-reaction assessment: IBV N as the antigen for the WB and ELISA..………………………………...…………………………………………….…81 Figure 14. Cross-reaction assessment: PDCoV N as the antigen for the WB and ELISA………………………………………………………………….……...………..83 Figure 15. Feline and human sera correlation.…………………….……...………..…..85 Figure 16. Mapping the cross-reactive region of SARS-CoV-2 N protein……..……...87 Figure 17. Coronavirus N protein sequence alignment and structure modeling………...89 Figure 18. Potential cross-reaction motifs: FCoV and SARS-CoV-2 N. ……..……….91 Figure 19. Potential cross-reaction motifs: FCoV and PCoV N.………………………..93 Figure 20. Potential cross-reaction motifs: SARS-CoV-2 and IBV N....………………94 List of tables Table 1. List of coronavirus strains used in this study……….……..……..…..………...96 Table 2. List of primers used in this study.…………..…………………….…………....97 Table 3. List of commercial antibodies used in this study……………..……..………....96 Table 4. Clinical sera and hyperimmune sera IgG titers..……...……..…..…………….99 Table 5. Analysis of serum relatedness………...……..…………….............................100 Table 6. Prediction of potential antigenic regions…..……………................................101 | |
| dc.language.iso | en | |
| dc.subject | 冠狀病毒 | zh_TW |
| dc.subject | 交叉反應 | zh_TW |
| dc.subject | 核殼蛋白 | zh_TW |
| dc.subject | 抗原性 | zh_TW |
| dc.subject | coronaviruses | en |
| dc.subject | antigenicity | en |
| dc.subject | cross-reaction | en |
| dc.subject | nucleocapsid protein | en |
| dc.title | 四種不同型別冠狀病毒核殼蛋白的交叉抗原性探討 | zh_TW |
| dc.title | Cross-antigenicity of nucleocapsid proteins in four different coronaviruses | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 110-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王金和(Ching-Ho Wang),張惠雯(Hui-Wen Chang),張世宗(Shih-Chung Chang) | |
| dc.subject.keyword | 冠狀病毒,核殼蛋白,抗原性,交叉反應, | zh_TW |
| dc.subject.keyword | coronaviruses,nucleocapsid protein,antigenicity,cross-reaction, | en |
| dc.relation.page | 109 | |
| dc.identifier.doi | 10.6342/NTU202202696 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2022-08-25 | |
| dc.contributor.author-college | 獸醫專業學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| dc.date.embargo-lift | 2022-08-26 | - |
| 顯示於系所單位： | 獸醫學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-2308202213224800.pdf | 14.46 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。