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標題: | 糞便指標菌在環境溫度上升時之適應性變化 Acclimation of Fecal Indicators Under Elevated Temperature |
作者: | Wei-Ting Hua 花葦庭 |
指導教授: | 童心欣(Hsin-Hsin Tung) 童心欣(Hsin-Hsin Tung | htung@ntu.edu.tw | ), |
關鍵字: | 高溫,總大腸菌群,糞大腸菌群,大腸桿菌,腸球菌, elevated temperature,total coliform,fecal coliform,Escherichia coli,enterococci, |
出版年 : | 2022 |
學位: | 碩士 |
摘要: | 隨著氣候的變化,氣溫升高引起了世界人類活動的關注。溫度的升高將影響環境水監測的許多不同方面。例如,旨在區分溫度依賴性生物的大腸菌群或糞便大腸菌群測試可能會受到環境溫度升高的影響。因此,本研究的目的是探索糞便指標菌在自然水域中的存活率,並確定高溫下大腸菌群/糞便大腸菌群對於糞便生物的貢獻/動態。本實驗從新店溪採集的水樣中加入牛糞,分別在 15、25 和 35°C下培養。使用滅菌後溪水與未添加牛糞的原始溪水做對照組在相同溫度下孵育,並定期監測大腸桿菌、總大腸菌群和糞大腸菌群。結果表明,無論溫度如何,糞便指標菌在淡水河水都有相似的衰減趨勢,而滅菌對照組會因水中沒有微生物競爭,使得糞便指標菌的存活率受溫度影響。整體下,添加糞源後的河水使糞便指標菌在培養中存活更長的時間。在本研究中,新店溪原水的水質參數(溶氧、濁度、pH、溫度和溶解性有機碳)與糞便指標菌的衰減沒有統計學上的相關性。但溶氧、濁度和溶解性有機碳的波動可能表明,當糞便污染進入河水時,糞便指標菌可能會由於水中含有細顆粒和高有機碳含量,達到延長存活時間。另外,溫度影響因子可能不是總大腸菌群、糞大腸菌群和大腸桿菌的最主要衰減因素。菌群死亡可能是由於與水中其他微生物的競爭造成的。因此,本研究結果顯示當大腸桿菌和腸球菌在水中共存時,由於兩項指標菌培養下對於新舊糞源汙染物有不同的衰退速率,故有機會幫助於管理水域水質並推測糞便污染事件時間進程。 With climate change, temperature increase has awakened concerns about anthropogenic activities for the world. The increasing temperature will impact many different aspects of environmental water monitoring. For example, coliform or fecal coliform tests, which are designed to differentiate temperature-dependent organisms, may be affected by elevated environmental temperature. Therefore, the objectives of this study were to explore the survival of fecal indicators in natural waters and identify the contribution/dynamics of fecal organisms in the coliform/fecal coliform testes under elevated temperatures. Water samples collected from Xindian creek spiked with bovine manure were incubated at 15, 25, and 35°C separately. Control groups with sterilized water or raw waters were also set at the same temperatures. Escherichia coli, total coliform, and fecal coliforms were monitored regularly during the experimental period. The result showed that raw water had a similar decay trend for fecal indicator bacteria (FIB) regardless of temperatures. FIB survival would be affected by temperature for sterilized control groups where no microbial competition was presented in water. Overall, seeded-raw water would permit more prolonged survival for fecal indicator organisms. Water quality parameters (DO, turbidity, pH, temperature, and DOC) are not statistically correlated with the decay of FIB for raw water in this study. But the fluctuation in DO, turbidity and DOC may indicate the possibility for prolonged survival of FIB when water was seeded with feces owing to fine particles and high organic carbon content. The subsequent experiments demonstrated that water temperature might not be the most important factor for the decay rates of total coliforms, fecal coliforms, and E. coli. Their die-off could be affected by the competition with waterborne microorganisms. Therefore, the information obtained from this study may be applicable for water quality management where E. coli and enterococci coexisted in water, exhibiting different decay rates to aged/fresh fecal pollutions. With fresh fecal pollution, both E. coli and enterococci should present in high numbers. However, in an aged pollution, only enterococci can be detected in high quantity after incubation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/85988 |
DOI: | 10.6342/NTU202203534 |
全文授權: | 同意授權(全球公開) |
電子全文公開日期: | 2024-09-30 |
顯示於系所單位: | 環境工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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U0001-1809202221192100.pdf 此日期後於網路公開 2024-09-30 | 2.3 MB | Adobe PDF |
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