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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃念祖(Nien-Tsu Huang) | |
dc.contributor.advisor | 黃念祖(Nien-Tsu Huang | nthuang@ntu.edu.tw | ), | |
dc.contributor.author | Zhi-Xuan Lai | en |
dc.contributor.author | 賴芷萱 | zh_TW |
dc.date.accessioned | 2023-03-19T22:13:10Z | - |
dc.date.copyright | 2022-10-20 | |
dc.date.issued | 2022 | |
dc.date.submitted | 2022-09-28 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/84487 | - |
dc.description.abstract | C-反應蛋白 (CRP) 是血漿中重要的發炎反應生物標記物,可用於診斷和監測心血管疾病 (CVD),心血管疾病長年位居全球第一大死因,若開發出可以精確量測 CRP 的定點照護 (POC) 裝置將有助於 CVD 的早期診斷。過去雖有許多文獻皆致力於開發可以精確檢測 CRP 的生物感測器,然而若要應用在 POC 環境則必須整合全血處理功能與原位生物感測,但目前僅有少數生物感測器具有整合血漿萃取功能,且未應用於 CRP 檢測。因此,在本篇論文中我們開發了整合血漿分離與電化學 CRP 檢測的微型化微流體平台。微流道部分整合了重力沉降及膜過濾兩種方式來優化血漿分離的效果。感測器部分則採用免標定的電化學生物感測器來進行原位 CRP 檢測,並優化感測器修飾步驟和電解液處理,以提高感測靈敏度及再現性。此外,為了能符合 POC 的需求,此系統的各項模組被設計的更為靈活且高度整合,在微型化的同時也更容易組裝和替換各模組元件。為驗證可行性,本研究使用此微型化微流體平台成功在 7 分鐘內從 400 µL 全血中萃取出 100 µL 無稀釋血漿,並接著使用內嵌的電化學 (EC) 感測器檢測血漿中的 CRP,檢測範圍涵蓋了臨床 CRP 濃度範圍 100 ng/mL 至 10 µg/mL。另外,該 EC 感測器也成功檢測出 1 ng/mL 至 10 µg/mL 範圍內的 CRP。上述的結果證明此高度整合的微流體平台具備應用於 POC 診斷的潛力。 | zh_TW |
dc.description.abstract | C-reactive protein (CRP) is an important inflammatory marker in plasma used for diagnosing cardiovascular diseases (CVDs), which have been the main cause of global mortality. A point-of-care (POC) diagnostic device for precise CRP determination can benefit the early-stage diagnosis of CVDs. Previously, various biosensors have been reported for CRP detection. However, to enable POC applications, the diagnostic device requires the integration of whole blood processing and in-situ biomarker detection. To the best of our knowledge, only a few POC-based biosensors integrate plasma extraction, and none has been reported for CRP detection. Therefore, in this thesis, we developed a miniaturized microfluidic platform integrating plasma separation with electrochemical CRP detection. To optimize the plasma separation performance, we integrate two blood separation features: (1) a sedimentation chamber and (2) a filter membrane in the microfluidic device. In the sensor part, we choose a label-free electrochemical biosensor for in-situ CRP detection. To achieve high sensitivity and reproducibility, the sensor modification and electrolyte handling protocols were optimized. Furthermore, all modules in the microfluidic platform were designed to be flexible, highly integrated, and miniaturized to achieve POC requirements. As a proof-of-concept, the microfluidic platform was successfully extracted 100 μL undiluted plasma from 400 µL whole blood within 7 min and subsequently detected CRP in the extracted plasma by an embedded electrochemical (EC) sensor in the clinical CRP range of 100 ng/mL to 10 µg/mL. In addition, the EC sensor successfully detected CRP in a broad range from 1 ng/mL to 10 µg/mL. In summary, the highly-integrated microfluidic platform shows great potential for POC diagnostics. | en |
dc.description.provenance | Made available in DSpace on 2023-03-19T22:13:10Z (GMT). No. of bitstreams: 1 U0001-2809202215341200.pdf: 3519744 bytes, checksum: b2e1eef3285b69b4897d597245709c0b (MD5) Previous issue date: 2022 | en |
dc.description.tableofcontents | CONTENTS 摘要 I Abstract II Chapter 1 Introduction 1 1.1 Research Background 1 1.1.1 Introduction of C-reactive Protein 1 1.1.2 The Importance of CRP as a Biomarker of Cardiovascular Diseases 2 1.1.3 Requirement of a Point-of-care Device for CRP Detection 3 1.2 Literature Review 4 1.2.1 Microfluidic Plasma Extraction Methods 4 1.2.2 Improved Strategies for Membrane-based Plasma Extraction 8 1.2.3 Electrochemical Biosensors Integrating with Plasma Extraction 11 1.3 Research Motivation 15 Chapter 2 Theory 16 2.1 Cyclic Voltammetry (CV) 16 2.2 Electrochemical Impedance Spectroscopy (EIS) 17 Chapter 3 Materials and Methods 21 3.1 Design and Preparation of the Microfluidic Platform 21 3.1.1 Design of the Microfluidic Platform 21 3.1.2 Introduction of the EC Sensor 23 3.1.3 Fabrication of the 3D microchannel 24 3.1.4 Operation of the Microfluidic Platform 26 3.2 EIS measurement 28 3.2.1 Preparation of the Detection Buffer 28 3.2.2 EIS Measurement Parameters and Analysis 29 3.3 Surface Cleaning and Modification 29 3.3.1 Surface Cleaning of the EC sensor 29 3.3.2 Surface Modification of the EC sensor 30 3.4 Biomarker detection 32 3.4.1 Detection of Antigens in PBS and Plasma 32 3.4.2 Detection of CRP in Whole Blood 33 Chapter 4 Results and Discussion 34 4.1 Validation of the EC Sensor 34 4.1.1 Verification of the Sensor Surface Modification 34 4.1.2 Evaluation of Detection Capability and Modification Reproducibility 36 4.1.3 Specificity 38 4.2 3D Microchannel Optimization and Validation 43 4.2.1 Design of the 3D Microchannel 43 4.2.2 Validation of Fluidic Control 46 4.2.3 Plasma Extraction Performance 47 4.3 Microfluidic Platform CRP Detection Performance 52 4.3.1 Detection Performance of CRP in PBS 52 4.3.2 Detection Performance of CRP in Plasma 55 4.3.3 Detection Performance of CRP in Whole Blood 58 Chapter 5 Conclusion 61 Chapter 6 Future Work 62 References 63 Appendix 67 | |
dc.language.iso | en | |
dc.title | 應用於無稀釋血漿分離及 C-反應蛋白電化學檢測之微流體平台 | zh_TW |
dc.title | A Microfluidic Platform for Undiluted Plasma Separation and Electrochemical Detection of C-Reactive Protein | en |
dc.type | Thesis | |
dc.date.schoolyear | 110-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林致廷(Chih-Ting Lin),陳建甫(Chien-Fu Chen),劉定宇(Ting-Yu Liu) | |
dc.subject.keyword | 微流體,全血處理,定點照護,電化學量測,心血管疾病, | zh_TW |
dc.subject.keyword | microfluidics,whole blood processing,point-of-care settings,electrochemical detection,cardiovascular disease, | en |
dc.relation.page | 69 | |
dc.identifier.doi | 10.6342/NTU202204203 | |
dc.rights.note | 同意授權(限校園內公開) | |
dc.date.accepted | 2022-09-29 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
dc.date.embargo-lift | 2022-10-20 | - |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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