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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/84194完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃義侑(Yi-You Huang) | |
| dc.contributor.author | KAI-WEN YANG | en |
| dc.contributor.author | 楊開文 | zh_TW |
| dc.date.accessioned | 2023-03-19T22:06:06Z | - |
| dc.date.copyright | 2022-07-05 | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2022-07-01 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/84194 | - |
| dc.description.abstract | 傷口癒合障礙是糖尿病患者最主要的死因。它是多種因素引發的病症，涉及到一系列內皮細胞功能障礙、糖尿病性神經病變、抵抗感染的能力受損等因素。目前臨床上主要是通過抗氧化劑，針對內皮的修復機制進行治療。但單靠傳統的抗氧化療法無法完全解決多種因素引起的糖尿病傷口癒合障礙。由於神經病變在糖尿病模型中至關重要，因此我們猜想是否能通過神經與內皮並行治療的策略，為糖尿病傷口提供一種新的治療可能。本研究選擇對神經保護與修復、血管再生均有益處的薑黃素，搭載於PLGA上形成藥物控制釋放體系，並結合水膠以形成複合型的藥物遞送系統，製成傷口敷料。期待能增強糖尿病傷口局部的抗氧化、抗炎症能力的同時，關注神經保護部分，達到早期治療的功效。 本研究合成出平均粒徑，凍乾前為190 nm，凍乾後為416 nm，但凍乾後粒徑分布更集中。薑黃素於PLGA上搭載率為10 ± 0.07 %。於體外試驗中，複合型水膠敷料能維持5天的完整性，同時顯現出藥物緩釋的能力並仍具有抗氧化性；於纖維母細胞、帶有神經脊細胞性質的嗜鉻細胞瘤細胞試驗中，均表現出無毒性，且能有效加速細胞爬行，具有促進生長的功效。於動物實驗中，搭載有薑黃素的水膠敷料在大鼠上表現出更佳的癒合能力。本研究證實了薑黃素水膠在糖尿病傷口模型中具有早期治療的潛力。 | zh_TW |
| dc.description.abstract | Impaired wound healing is the major causes of death in diabetic patients. It is involved endothelial cell dysfunction, diabetic neuropathy and increased risk of infection. At present, the main clinical treatment is antioxidants, aiming at the repair mechanism of the endothelium. However, there is no single therapy to date can fully treat diabetes-induced wound caused by multiple mechanisms. A strategy of parallel treatment between nerves and endothelium may provide a new therapeutic possibility for diabetic wounds. In this study, curcumin was used due to its antioxidant, anti-inflammatory, antibacterial properties. PLGA loaded with curcumin as a drug controlled-release system, combined with sodium alginate were made for wound dressings. The average particle size was 190 nm and 416 nm, before and after freeze-drying, but polydispersity index decreased to 0.005. The loading rate of curcumin on PLGA was 10 ± 0.07 %. In vitro analysis, the dressings maintained for 5 days and showed the ability of sustained drug release, still with antioxidant; biocompatibility tests showed no toxicity on fibroblasts and PC-12. It accelerated cell crawling effectively. In vivo analysis, dressings loaded with curcumin showed better healing ability. This study confirmed the early therapeutic potential of curcumin hydrogel in a diabetic wound model. | en |
| dc.description.provenance | Made available in DSpace on 2023-03-19T22:06:06Z (GMT). No. of bitstreams: 1 U0001-2806202213443600.pdf: 5423690 bytes, checksum: 8170494fbc236df39a129a245ee159ca (MD5) Previous issue date: 2022 | en |
| dc.description.tableofcontents | 目 錄 口試委員審定書 i 致謝 ii 摘要 iii Abstract iv 第一章 糖尿病與傷口癒合障礙 1 1.1 糖尿病 1 1.2 糖尿病的傷口癒合障礙 1 1.2.1 內皮細胞功能障礙 2 1.2.2 糖尿病性神經病變 3 1.2.3 傷口癒合障礙 4 1.2.4 治療方式 8 第二章 研究概述 11 2.1 研究動機與目的 11 2.2 藥物的選擇策略 11 2.3 藥物載體的的選擇策略 14 2.3.1 聚乳酸-甘醇酸 14 2.3.2 海藻酸钠 15 2.4 實驗流程圖 17 第三章 實驗材料及方法 18 3.1 實驗試劑 18 3.2 實驗儀器 18 3.3 材料製備 19 3.3.1 搭載薑黃素之PLGA奈米顆粒 19 3.3.2 複合型藥物傳輸體系──搭載薑黃素之PLGA-藻酸鹽水膠敷料 21 3.4 材料分析 22 3.4.1 搭載薑黃素之PLGA奈米顆粒基礎特性分析 22 3.4.1.1 動態光散射分析 (Dynamic Light Scattering, DLS) 22 3.4.1.2 掃描式電子顯微鏡分析(Scanning Electron Microscope, SEM) 22 3.4.1.3 薑黃素載藥率測定 22 3.4.2 PLGA搭載薑黃素之水膠敷料特性分析 23 3.4.3 體外試驗 23 3.4.3.1 降解/膨潤分析 23 3.4.3.2 體外水膠球藥物控釋分析 23 3.4.3.3 體外抗氧化試驗 24 3.4.3.4 體外細胞毒性分析 25 3.4.3.5 體外細胞遷移/傷口癒合測試 26 3.4.4 體內試驗 27 3.4.4.1 糖尿病大鼠模型建立 27 3.4.4.2 糖尿病大鼠模型傷口建立 27 3.4.4.3 組織學 28 3.5 統計分析 28 第四章 實驗結果與討論 29 4.1 搭載薑黃素之PLGA奈米顆粒基礎特性分析與討論 29 4.1.1 水合粒徑分布及Zeta電位分析 29 4.1.2 掃描式電子顯微鏡分析 30 4.1.3 薑黃素搭載率 31 4.2 PLGA搭載薑黃素之水膠球特性分析 31 4.2.1 體外試驗 31 4.2.1.1 降解/膨潤分析 31 4.2.1.2 體外敷料藥物控釋分析 33 4.2.1.3 體外抗氧化試驗 34 4.2.1.4 體外細胞毒性分析 35 4.2.1.5 體外細胞遷移/傷口癒合測試 38 4.2.2 體內試驗 42 4.2.2.1 體內細胞遷移/傷口癒合測試 42 4.2.2.2 組織學 44 第五章 結論 47 參考文獻 49 附錄Ⅰ常用英文縮寫對照表 57 附錄Ⅱ 切除傷口癒合的組織學評分標準 60 | |
| dc.language.iso | zh-TW | |
| dc.subject | 糖尿病 | zh_TW |
| dc.subject | 傷口癒合 | zh_TW |
| dc.subject | 薑黃素 | zh_TW |
| dc.subject | 聚乳酸甘醇酸 | zh_TW |
| dc.subject | 海藻酸鈉 | zh_TW |
| dc.subject | 傷口敷料 | zh_TW |
| dc.subject | wound dressing | en |
| dc.subject | diabetes | en |
| dc.subject | wound healing | en |
| dc.subject | curcumin | en |
| dc.subject | PLGA | en |
| dc.subject | alginate | en |
| dc.title | 搭載薑黃素之藻酸鹽PLGA複合水膠敷料於糖尿病傷口治療上的應用 | zh_TW |
| dc.title | Application of Alginate-PLGA Composite Dressing for the Treatment of Diabetes Mellitus | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 110-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許馨云(Hsin-Yun Hsu),黃意真(Yi-Cheng Huang) | |
| dc.subject.keyword | 糖尿病,傷口癒合,薑黃素,聚乳酸甘醇酸,海藻酸鈉,傷口敷料, | zh_TW |
| dc.subject.keyword | diabetes,wound healing,curcumin,PLGA,alginate,wound dressing, | en |
| dc.relation.page | 61 | |
| dc.identifier.doi | 10.6342/NTU202201174 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2022-07-03 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| dc.date.embargo-lift | 2022-07-05 | - |
| 顯示於系所單位： | 醫學工程學研究所 | |
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