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標題: | 線蟲內由不同飲食調控的脂質代謝 C. elegans lipid metabolism mediated by different bacterial diets |
作者: | Erh-Ya Lin 林爾雅 |
指導教授: | 吳益群(Yi-Chun Wu) 吳益群(Yi-Chun Wu | yichun@ntu.edu.tw | ), |
關鍵字: | 飲食,類溶?體相關胞器,脂質代謝,鞘脂,秀麗隱桿線蟲, diet,lysosome related organelle,lipid metabolism,sphingolipid,Caenorhabditis elegans, |
出版年 : | 2022 |
學位: | 碩士 |
摘要: | 飲食提供生物體營養及能量。多餘的能量會被儲存成脂質且脂質恆定不平衡會造成脂肪累積導致代謝疾病。因此,了解飲食如何影響生物體中的脂質代謝就顯得重要。類溶?體相關胞器(LRO)是一種和溶?體有許多相似特徵的酸性胞器並被發現存在於秀麗隱桿線蟲到人類等生物體中。然而,它的功能尚不明確。在本篇研究中,我們探討了線蟲中的類溶?體相關胞器如何透過不同的細菌飲食-OP50及DA1877影響脂質代謝。我們發現在位於酸性胞器中的一個酸性脂肪?在不同飲食中mRNA表現量有顯著的改變。除此之外,我們證明了在DA飲食下該脂肪?參與在類溶?體相關胞器數量、磷脂醯膽鹼及三酸甘油脂的表現量的調控。另一方面,鞘脂的代謝產物參與在磷脂醯膽鹼的合成中,我們也發現飲食會影響到鞘脂的含量。而鞘脂的恆定在DA飲食的線蟲中至關重要,可能是由於它參與磷脂醯膽鹼的合成。我的研究揭示了從類溶?體相關胞器到磷脂醯膽鹼和脂質代謝的信號通路,並將提供飲食對慢性疾病(如肥胖)的病因和預防的見解。 Diets provide nutrients and energy for organisms. Excessive energy would be stored as lipid and Imbalanced lipid homeostasis causing fat accumulation would lead to metabolic disorders. Therefore, understanding how diets regulate organismal lipid metabolism is important. Lysosome-related organelle(LRO) is an acidic organelle which shares many features with the lysosome and is found from C. elegans to human. However, their functions are not well characterized. In this work, we examined how LROs influence lipid metabolism in C. elegans by different bacterial diets, OP50 and DA1877. We found a specific acid lipase, which is localized to acidic organelles, showed a significant change at the mRNA level in response to different diets. Furthermore, we demonstrated that the acid lipase is involved in the regulation of LRO number and triacylglycerol level in response to DA diet. Interestingly, the results of lipidomics profiling showed that this lipase is required to promote the phosphatidylcholine level in the DA diet. On the other hand, we also found that the two bacterial diets affect sphingolipid metabolism, possibly due to its involvement in PC synthesis. My work reveals a novel signaling pathway from LRO to PC and lipid metabolism, and would provide insights into the dietary implication in the cause and prevention of chronic diseases, such as obesity. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/84057 |
DOI: | 10.6342/NTU202203840 |
全文授權: | 未授權 |
顯示於系所單位: | 分子與細胞生物學研究所 |
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