請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/83587
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 曲芳華 | zh_TW |
dc.contributor.advisor | Fang-Hua Chu | en |
dc.contributor.author | 陳廷瑋 | zh_TW |
dc.contributor.author | Ting-Wei Chen | en |
dc.date.accessioned | 2023-03-19T21:11:13Z | - |
dc.date.available | 2023-12-26 | - |
dc.date.copyright | 2022-08-31 | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2002-01-01 | - |
dc.identifier.citation | Adams, R. P. (2007). Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry (Vol. 456, pp. 544-545). Carol Stream: Allured publishing corporation. Aladedunye, F. A., Benn, M. H., & Okorie, D. A. (2008). Sesquiterpenes from Culcasia scandens P. Beauv. Natural Product Research, 22(10), 879–883. Alicandri, E., Covino, S., Sebastiani, B., Paolacci, A. R., Badiani, M., Sorgonà, A., & Ciaffi, M. (2022). Monoterpene Synthase Genes and Monoterpene Profiles in Pinus nigra subsp. Laricio. Plants, 11(3), 449. Alicandri, E., Paolacci, A. R., Osadolor, S., Sorgonà, A., Badiani, M., & Ciaffi, M. (2020). On the Evolution and Functional Diversity of Terpene Synthases in the Pinus Species: A Review. Journal of Molecular Evolution, 88(3), 253–283. Alquézar, B., Rodríguez, A., de la Peña, M., & Peña, L. (2017). Genomic Analysis of Terpene Synthase Family and Functional Characterization of Seven Sesquiterpene Synthases from Citrus sinensis. Frontiers in Plant Science, 8, 1481. Arimura, G., Garms, S., Maffei, M., Bossi, S., Schulze, B., Leitner, M., Mithöfer, A., & Boland, W. (2008). Herbivore-induced Terpenoid Emission in Medicago truncatula: Concerted Action of Jasmonate, Ethylene and Calcium Signaling. Planta, 227(2), 453–464. Arimura, G., Ozawa, R., Kugimiya, S., Takabayashi, J., & Bohlmann, J. (2004). Herbivore-Induced Defense Response in a Model Legume. Two-Spotted Spider Mites Induce Emission of (E)-β-Ocimene and Transcript Accumulation of (E)-β-Ocimene Synthase in Lotus japonicus. Plant Physiology, 135(4), 1976–1983. Arimura, G., Ozawa, R., Shimoda, T., Nishioka, T., Boland, W., & Takabayashi, J. (2000). Herbivory-induced Volatiles Elicit Defence Genes in Lima Bean lLeaves. Nature, 406(6795), 512–515. Aubourg, S., Lecharny, A., & Bohlmann, J. (2002). Genomic Analysis of the Terpenoid Synthase (AtTPS) Gene Family of Arabidopsis thaliana. Molecular Genetics and Genomics, 267(6), 730–745. Bohlmann, J., Crock, J., Jetter, R., & Croteau, R. (1998a). Terpenoid-based Defenses in Conifers: cDNA Cloning, Characterization, and Functional Expression of Wound-inducible (E)-α-Bisabolene Synthase from Grand fir (Abies grandis). Proceedings of the National Academy of Sciences, 95(12), 6756–6761. Bohlmann, J., Meyer-Gauen, G., & Croteau, R. (1998b). Plant Terpenoid Synthases: Molecular Biology and Phylogenetic Analysis. Proceedings of the National Academy of Sciences, 95(8), 4126–4133. Bohlmann, J., Phillips, M., Ramachandiran, V., Katoh, S., & Croteau, R. (1999). cDNA Cloning, Characterization, and Functional Expression of Four New Monoterpene Synthase Members of the Tpsd Gene Family from Grand Fir (Abies grandis). Archives of Biochemistry and Biophysics, 368(2), 232–243. Brillada, C., Nishihara, M., Shimoda, T., Garms, S., Boland, W., Maffei, M. E., & Arimura, G. (2013). Metabolic Engineering of The C16 Homoterpene TMTT in Lotus japonicus Through Overexpression of (E,E)-Geranyllinalool Synthase Attracts Generalist and Specialist Predators in Different Manners. New Phytologist, 200(4), 1200–1211. Byers, K. J. R. P., Bradshaw, H. D., Jr, & Riffell, J. A. (2014). Three Floral Volatiles Contribute to Differential Pollinator Attraction in Monkeyflowers (Mimulus). Journal of Experimental Biology, 217(4), 614–623. Caputi, L., & Aprea, E. (2011). Use of Terpenoids as Natural Flavouring Compounds in Food Industry. Recent Patents on Food, Nutrition & Agriculture, 3(1), 9–16 Chang, S., Puryear, J., & Cairney, J. (1993). A Simple and Efficient Method for Isolating RNA from Pine Trees. Plant Molecular Biology Reporter, 11(2), 113–116. Chen, F., Tholl, D., Bohlmann, J., & Pichersky, E. (2011). The Family of Terpene Synthases in Plants: A Mid-size Family of Genes for Specialized Metabolism That is Highly Diversified Throughout the Kingdom. The Plant Journal, 66(1), 212–229. Chen, Y., & Chang, S. (2017). Distribution and Characteristic Comparisons of the Endemic Cypresses in Taiwan. Taiwan Journal of Forest Science, 32(1), 71-86. Chen, T. L., Hong, P. F., Lin, Y. C., & Huang, J. C. (2009). Visual Image Analysis of Alishan Five-wood Species in Taiwan. Forest Products Industries, 28(1):13- 26. Cheng, S.-S., Chang, H.-T., Wu, C.-L., & Chang, S.-T. (2007). Anti-termitic Activities of Essential Oils from Coniferous Trees against Coptotermes formosanus. Bioresource Technology, 98(2), 456–459. Chi, W. T. (2008). Between Empowerment and Co-management: A Preliminary Study on the Management of “Ma-Guo National Park” in Cultural Industry. Taiwan Heritages, 45:105–120. Chien, T.-C., Lo, S.-F., & Ho, C.-L. (2014). Chemical Composition and Anti-inflammatory Activity of Chamaecyparis obtusa f. Formosana Wood Essential Oil from Taiwan. Natural Product Communications, 9(5), 723–726. Christianson, D. W. (2017). Structural and Chemical Biology of Terpenoid Cyclases. Chemical Reviews, 117(17), 11570–11648. Chung, M.-J., Cheng, S.-S., Lin, C.-Y., & Chang, S.-T. (2018). Profiling of Volatile Compounds from Five Interior Decoration Timbers in Taiwan Using TD/GC–MS/FID. Journal of Wood Science, 64(6), 823–835. Cimmino, A., Andolfi, A., & Evidente, A. (2014). Phytotoxic Terpenes Produced by Phytopathogenic Fungi and Allelopathic Plants. Natural Product Communications, 9(3), 401–408. Cool, L. G. (2005). Sesquiterpenes from Cupressus macrocarpa foliage. Phytochemistry, 66(2), 249–260. Crock, J., Wildung, M., & Croteau, R. (1997). Isolation and Bacterial Expression of A Sesquiterpene Synthase cDNA Clone from Peppermint (Mentha X Piperita, L.) That Produces the Aphid Alarm Pheromone (E)-β-Farnesene. Proceedings of the National Academy of Sciences, 94(24), 12833–12838. de Kraker, J. W., Franssen, M. C. R., de Groot, A., König, W. A., & Bouwmeester, H. J. (1998). (+)-Germacrene A Biosynthesis: The Committed Step in the Biosynthesis of Bitter Sesquiterpene Lactones in Chicory. Plant Physiology, 117(4), 1381–1392. Degenhardt, J., Köllner, T. G., & Gershenzon, J. (2009). Monoterpene and Sesquiterpene Synthases and the Origin of Terpene Skeletal Diversity in Plants. Phytochemistry, 70(15), 1621–1637. Dickschat, J. S., Celik, E., & Brock, N. L. (2018). Volatiles from Three Genome Sequenced Fungi from the Genus Aspergillus. Beilstein Journal of Organic Chemistry, 14(1), 900–910. Dudareva, N., Klempien, A., Muhlemann, J. K., & Kaplan, I. (2013). Biosynthesis, Function and Metabolic Engineering of Plant Volatile Organic Compounds. New Phytologist, 198(1), 16–32. Dudareva, N., Martin, D., Kish, C. M., Kolosova, N., Gorenstein, N., Fäldt, J., Miller, B., & Bohlmann, J. (2003). (E)-β-Ocimene and Myrcene Synthase Genes of Floral Scent Biosynthesis in Snapdragon: Function and Expression of Three Terpene Synthase Genes of a New Terpene Synthase Subfamily. The Plant Cell, 15(5), 1227–1241. Dudareva, N., Pichersky, E., & Gershenzon, J. (2004). Biochemistry of Plant Volatiles. Plant Physiology, 135(4), 1893–1902. Durairaj, J., Di Girolamo, A., Bouwmeester, H. J., de Ridder, D., Beekwilder, J., & van Dijk, A. DJ. (2019). An Analysis of Characterized Plant Sesquiterpene Synthases. Phytochemistry, 158, 157–165. Durairaj, J., Melillo, E., Bouwmeester, H. J., Beekwilder, J., Ridder, D. de, & Dijk, A. D. J. van. (2021). Integrating Structure-based Machine Learning and co-Evolution to Investigate Specificity in Plant Sesquiterpene Synthases. PLOS Computational Biology, 17(3), e1008197. Fäldt, J., Arimura, G., Gershenzon, J., Takabayashi, J., & Bohlmann, J. (2003). Functional Identification of AtTPS03 as (E)-β-Ocimene Synthase: A Monoterpene Synthase Catalyzing Jasmonate- and Wound-induced Volatile Formation in Arabidopsis thaliana. Planta, 216(5), 745–751. Foster, A. J., Hall, D. E., Mortimer, L., Abercromby, S., Gries, R., Gries, G., Bohlmann, J., Russell, J., & Mattsson, J. (2013). Identification of Genes in Thuja plicata Foliar Terpenoid Defenses. Plant Physiology, 161(4), 1993–2004. Hayashi, K., Kawaide, H., Notomi, M., Sakigi, Y., Matsuo, A., & Nozaki, H. (2006). Identification and Functional Analysis of Bifunctional ent-Kaurene Synthase from The Moss Physcomitrella patens. FEBS Letters, 580(26), 6175–6181. Hillwig, M. L., Xu, M., Toyomasu, T., Tiernan, M. S., Wei, G., Cui, G., Huang, L., & Peters, R. J. (2011). Domain Loss Has Independently Occurred Multiple Times in Plant Terpene Synthase Evolution. The Plant Journal, 68(6), 1051–1060. Hong, C.-Y., Tsao, N.-W., Wang, S.-Y., & Chu, F.-H. (2022). Cloning and Functional Characterization of Three Sesquiterpene Synthase Genes from Chamaecyparis formosensis Matsumura. Plant Science, 321, 111315. Huang, C.-J., Chu, F.-H., Huang, Y.-S., Hung, Y.-M., Tseng, Y.-H., Pu, C.-E., Chao, C.-H., Chou, Y.-S., Liu, S.-C., You, Y. T., Hsu, S.-Y., Hsieh, H.-C., Hsu, C. T., Chen, M.-Y., Lin, T.-A., Shyu, H.-Y., Tu, Y.-C., & Chen, C.-T. (2020). Development and Technical Application of SSR-based Individual Identification System for Chamaecyparis taiwanensis against Illegal Logging Convictions. Scientific Reports, 10(1), 1–14. Hyatt, D. C., Youn, B., Zhao, Y., Santhamma, B., Coates, R. M., Croteau, R. B., & Kang, C. (2007). Structure of Limonene Synthase, A Simple Model for Terpenoid Cyclase Catalysis. Proceedings of the National Academy of Sciences, 104(13), 5360–5365. Iijima, Y., Gang, D. R., Fridman, E., Lewinsohn, E., & Pichersky, E. (2004). Characterization of Geraniol Synthase from the Peltate Glands of Sweet Basil. Plant Physiology, 134(1), 370–379. Ilc, T., Parage, C., Boachon, B., Navrot, N., & Werck-Reichhart, D. (2016). Monoterpenol Oxidative Metabolism: Role in Plant Adaptation and Potential Applications. Frontiers in Plant Science, 7, 509. Inamori, Y., Shinohara, S., Tsujibo, H., Okabe, T., Morita, Y., Sakagami, Y., Kumeda, Y., & Ishida, N. (1999). Antimicrobial Activity and Metalloprotease Inhibition of Hinokitiol-Related Compounds, the Constituents of Thujopsis dolabrata S. and Z. hondai MAK. Biological & Pharmaceutical Bulletin, 22(9), 990–993. Jayakumar, T., Liu, C.-H., Wu, G.-Y., Lee, T.-Y., Manubolu, M., Hsieh, C.-Y., Yang, C.-H., & Sheu, J.-R. (2018). Hinokitiol Inhibits Migration of A549 Lung Cancer Cells via Suppression of MMPs and Induction of Antioxidant Enzymes and Apoptosis. International Journal of Molecular Sciences, 19(4), 939. Jia, Q., Brown, R., Köllner, T. G., Fu, J., Chen, X., Wong, G. K.-S., Gershenzon, J., Peters, R. J., & Chen, F. (2022). Origin and Early Evolution of the Plant Terpene Synthase Family. Proceedings of the National Academy of Sciences, 119(15), e2100361119. Kampranis, S. C., Ioannidis, D., Purvis, A., Mahrez, W., Ninga, E., Katerelos, N. A., Anssour, S., Dunwell, J. M., Degenhardt, J., Makris, A. M., Goodenough, P. W., & Johnson, C. B. (2007). Rational Conversion of Substrate and Product Specificity in A Salvia Monoterpene Synthase: Structural Insights into the Evolution of Terpene Synthase Function. The Plant Cell, 19(6), 1994–2005. Karunanithi, P. S., & Zerbe, P. (2019). Terpene Synthases as Metabolic Gatekeepers in the Evolution of Plant Terpenoid Chemical Diversity. Frontiers in Plant Science, 10, 1166. Kasuya, H., Hata, E., Satou, T., Yoshikawa, M., Hayashi, S., Masuo, Y., & Koike, K. (2013). Effect on Emotional Behavior and Stress by Inhalation of the Essential oil from Chamaecyparis obtusa. Natural Product Communications, 8(4), 515-518. Keeling, C. I., & Bohlmann, J. (2006). Genes, Enzymes and Chemicals of Terpenoid Diversity in the Constitutive and Induced Defence of Conifers against Insects and Pathogens. New Phytologist, 170(4), 657–675. Keeling, C. I., Weisshaar, S., Ralph, S. G., Jancsik, S., Hamberger, B., Dullat, H. K., & Bohlmann, J. (2011). Transcriptome Mining, Functional Characterization, and Phylogeny of a Large Terpene Synthase Gene Family in Spruce (Picea spp.). BMC Plant Biology, 11(1), 43. Knudsen, J. T., Eriksson, R., Gershenzon, J., & Ståhl, B. (2006). Diversity and Distribution of Floral Scent. The Botanical Review, 72(1), 1. Koo, H. J., Vickery, C. R., Xu, Y., Louie, G. V., O’Maille, P. E., Bowman, M., Nartey, C. M., Burkart, M. D., & Noel, J. P. (2016). Biosynthetic Potential of Sesquiterpene Synthases: Product Profiles of Egyptian Henbane Premnaspirodiene Synthase and Related Mutants. The Journal of Antibiotics, 69(7), 524–533. Köpke, D., Schröder, R., Fischer, H. M., Gershenzon, J., Hilker, M., & Schmidt, A. (2008). Does Egg Deposition by Herbivorous Pine Sawflies Affect Transcription of Sesquiterpene Synthases in Pine? Planta, 228(3), 427–438. Krenn, B. M., Gaudernak, E., Holzer, B., Lanke, K., Van Kuppeveld, F. J. M., & Seipelt, J. (2009). Antiviral Activity of the Zinc Ionophores Pyrithione and Hinokitiol against Picornavirus Infections. Journal of Virology, 83(1), 58–64. Kuo, Y. H., Chen, C. H., Chien, S. C., & Lin, Y. L. (2002). Five New Cadinane-type Sesquiterpenes from the Heartwood of Chamaecyparis obtusa var. formosana. Journal of natural products, 65(1), 25–28. Kurosawa, S., Bando, M., & Mori, K. (2001). Synthesis of (1R,4R,5S)-(+)-Acoradiene, the Structure Proposed for the Aggregation Pheromone of the Broad-Horned Flour Beetle. European Journal of Organic Chemistry, 2001(23), 4395–4399. Li, R., Wang, K., Wang, D., Xu, L., Shi, Y., Dai, Z., & Zhang, X. (2021a). Production of Plant Volatile Terpenoids (Rose Oil) by Yeast Cell Factories. Green Chemistry, 23(14), 5088–5096. Li, W., Yan, X., Zhang, Y., Liang, D., Caiyin, Q., & Qiao, J. (2021b). Characterization of trans-Nerolidol Synthase from Celastrus Angulatus Maxim and Production of trans-Nerolidol in Engineered Saccharomyces cerevisiae. Journal of Agricultural and Food Chemistry, 69(7), 2236–2244. Liang, D., Li, W., Yan, X., Caiyin, Q., Zhao, G., & Qiao, J. (2021). Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases. International Journal of Molecular Sciences, 22(12), 6348. Lin, Z. J. (1988). The Silviculture of Chamaecyparis obtusa var. fomosana. Mod Silvicult 3(2):29–32. Liu, Y., Chen, J., Qian, J., Lin, H., Sun, N., & Huang, Z. (2018). Evolutionary Analysis and Structural Characterization of Aquilaria sinensis Sesquiterpene Synthase in Agarwood Formation: A computational Study. Journal of Theoretical Biology, 456, 249–260. Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., & Verhoeven, H. A. (2002). Monoterpene Biosynthesis in Lemon (Citrus limon). European Journal of Biochemistry, 269(13), 3160–3171. Ma, L.-T., Lee, Y.-R., Liu, P.-L., Cheng, Y.-T., Shiu, T.-F., Tsao, N.-W., Wang, S.-Y., & Chu, F.-H. (2019a). Phylogenetically Distant Group of Terpene Synthases Participates in Cadinene and Cedrane-type Sesquiterpenes Accumulation in Taiwania cryptomerioides. Plant Science, 289, 110277. Ma, L.-T., Lee, Y.-R., Tsao, N.-W., Wang, S.-Y., Zerbe, P., & Chu, F.-H. (2019b). Biochemical Characterization of Diterpene Synthases of Taiwania cryptomerioides Expands the Known Functional Space of Specialized Diterpene Metabolism in Gymnosperms. The Plant Journal, 100(6), 1254–1272. Ma, L.-T., Liu, P.-L., Cheng, Y.-T., Shiu, T.-F., & Chu, F.-H. (2021). Unveiling Monoterpene Biosynthesis in Taiwania cryptomerioides via Functional Characterization. Plants, 10(11), 2404. Martin, D. M., Aubourg, S., Schouwey, M. B., Daviet, L., Schalk, M., Toub, O., Lund, S. T., & Bohlmann, J. (2010). Functional Annotation, Genome Organization and Phylogeny of the Grapevine (Vitis vinifera) Terpene Synthase Gene Family Based on Genome Assembly, FLcDNA Cloning, and Enzyme Assays. BMC Plant Biology, 10(1), 226. Martin, D. M., Fäldt, J., & Bohlmann, J. (2004). Functional Characterization of Nine Norway Spruce TPS Genes and Evolution of Gymnosperm Terpene Synthases of the TPS-d Subfamily. Plant Physiology, 135(4), 1908–1927. Mazzoni, V., Tomi, F., & Casanova, J. (1999). A Daucane-type Sesquiterpene from Daucus carota Seed Oil. Flavour and Fragrance Journal, 14(5), 268–272. Morrone, D., Chambers, J., Lowry, L., Kim, G., Anterola, A., Bender, K., & Peters, R. J. (2009). Gibberellin Biosynthesis in Bacteria: Separate ent-Copalyl Diphosphate and ent-Kaurene Synthases in Bradyrhizobium japonicum. FEBS Letters, 583(2), 475–480. Nagegowda, D. A. (2010). Plant Volatile Terpenoid Metabolism: Biosynthetic Genes, Transcriptional Regulation and Subcellular Compartmentation. FEBS Letters, 584(14), 2965–2973. Nicolaou, K. C., Dai, W.-M., & Guy, R. K. (1994). Chemistry and Biology of Taxol. Angewandte Chemie International Edition in English, 33(1), 15–44. Pazouki, L., & Niinemets, Ü. (2016). Multi-Substrate Terpene Synthases: Their Occurrence and Physiological Significance. Frontiers in Plant Science, 7, 1019. Peters, R. J., Carter, O. A., Zhang, Y., Matthews, B. W., & Croteau, R. B. (2003). Bifunctional Abietadiene Synthase: Mutual Structural Dependence of the Active Sites for Protonation-Initiated and Ionization-Initiated Cyclizations. Biochemistry, 42(9), 2700–2707. Roach, C. R., Hall, D. E., Zerbe, P., & Bohlmann, J. (2014). Plasticity and Evolution of (+)-3-Carene Synthase and (−)-Sabinene Synthase Functions of a Sitka Spruce Monoterpene Synthase Gene Family Associated with Weevil Resistance. Journal of Biological Chemistry, 289(34), 23859–23869. Rodrigues, A. C. B. C., Bomfim, L. M., Neves, S. P., Menezes, L. R. A., Dias, R. B., Soares, M. B. P., Prata, A. P. N., Rocha, C. A. G., Costa, E. V., & Bezerra, D. P. (2015). Antitumor Properties of the Essential Oil from the Leaves of Duguetia gardneriana. Planta Medica, 81(10), 798–803. Rodrı́guez-Concepción, M., & Boronat, A. (2002). Elucidation of the Methylerythritol Phosphate Pathway for Isoprenoid Biosynthesis in Bacteria and Plastids. A Metabolic Milestone Achieved through Genomics. Plant Physiology, 130(3), 1079–1089. Saniewski, M., Saniewska, A., & Kanlayanarat, S. (2007). Biological Activities of Tropolone and Hinokitiol: The Tools in Plant Physiology and Their Practical Use. Acta Horticulturae, 755, 133–142. Schmidt-Dannert, C. (2015). Biosynthesis of Terpenoid Natural Products in Fungi. In J. Schrader & J. Bohlmann (Eds.), Biotechnology of Isoprenoids (pp. 19–61). Springer International Publishing. Sharkey, T. D., Gray, D. W., Pell, H. K., Breneman, S. R., & Topper, L. (2013). Isoprene Synthase Genes Form a Monophyletic Clade of Acyclic Terpene Synthases in the Tps-B Terpene Synthase Family. Evolution, 67(4), 1026–1040. Steele, C. L., Crock, J., Bohlmann, J., & Croteau, R. (1998). Sesquiterpene Synthases from Grand Fir (Abies grandis): Comparison of Constitutive and Wound-Induced Activities, and cDNA Isolation, Characterization, And Bacterial Expression of δ-Selinene Synthase and γ-Humulene Synthase. Journal of Biological Chemistry, 273(4), 2078–2089. Su, P., Hu, T., Liu, Y., Tong, Y., Guan, H., Zhang, Y., Zhou, J., Huang, L., & Gao, W. (2017). Functional Characterization of NES and GES Responsible for the Biosynthesis of (E)-Nerolidol and (E,E)-Geranyllinalool in Tripterygium wilfordii. Scientific Reports, 7(1), 40851. Tholl, D. (2015). Biosynthesis and Biological Functions of Terpenoids in Plants. In J. Schrader & J. Bohlmann (Eds.), Biotechnology of Isoprenoids (pp. 63–106). Springer International Publishing. Trapp, S. C., & Croteau, R. B. (2001). Genomic Organization of Plant Terpene Synthases and Molecular Evolutionary Implications. Genetics, 158(2), 811–832. Trindade, H., Pedro, L. G., Figueiredo, A. C., & Barroso, J. G. (2018). Chemotypes and Terpene Synthase Genes in Thymus genus: State of the Art. Industrial Crops and Products, 124, 530–547. Tu, D.-G., Yu, Y., Lee, C.-H., Kuo, Y.-L., Lu, Y.-C., Tu, C.-W., & Chang, W.-W. (2016). Hinokitiol Inhibits Vasculogenic Mimicry Activity of Breast Cancer Stem/Progenitor Cells Through Proteasome‑mediated Degradation of Epidermal Growth Factor Receptor. Oncology Letters, 11(4), 2934–2940. Velikova, V., Ghirardo, A., Vanzo, E., Merl, J., Hauck, S. M., & Schnitzler, J.-P. (2014). Genetic Manipulation of Isoprene Emissions in Poplar Plants Remodels the Chloroplast Proteome. Journal of Proteome Research, 13(4), 2005–2018. Wang, C., Zhou, J., Jang, H.-J., Yoon, S.-H., Kim, J.-Y., Lee, S.-G., Choi, E.-S., & Kim, S.-W. (2013). Engineered Heterologous FPP Synthases-mediated Z,E-FPP Synthesis in E. coli. Metabolic Engineering, 18, 53–59. White, N. J. (1997). Assessment of the Pharmacodynamic Properties of Antimalarial Drugs in vivo. Antimicrobial Agents and Chemotherapy, 41(7), 1413–1422. Ye, J., Xu, Y.-F., Lou, L.-X., Jin, K., Miao, Q., Ye, X., & Xi, Y. (2015). Anti-inflammatory Effects of Hinokitiol on Human Corneal Epithelial Cells: An in vitro study. Eye, 29(7), 964–971. Zhou, K., & Peters, R. J. (2009). Investigating the Conservation Pattern of a Putative Second Terpene Synthase Divalent Metal Binding Motif in Plants. Phytochemistry, 70(3), 366–369. Zulak, K. G., Lippert, D. N., Kuzyk, M. A., Domanski, D., Chou, T., Borchers, C. H., & Bohlmann, J. (2009). Targeted Proteomics Using Selected Reaction Monitoring Reveals the Induction of Specific Terpene Synthases in a Multi-level Study of Methyl Jasmonate-treated Norway spruce (Picea abies). The Plant Journal, 60(6), 1015–1030. | - |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/83587 | - |
dc.description.abstract | 臺灣扁柏 (Chamaecyparis obtusa var. formosana),是臺灣珍貴的特有種植物,為日本扁柏 (Chamaecyparis obtusa) 的變種。在利用上非常的廣泛,具有良好質地及材色的主幹適合作為建材和家具,並且整株植物皆富含香氣,可製作成精油及芳香劑等一系列產品。在精油的組成中以揮發性萜類化合物為主,其中不乏只有在臺灣扁柏中才能發現的化合物,為了更加清楚這些產物的生合成機制,因此對其合成酶做更進一步的研究。然而,現今並沒有對於臺灣扁柏萜類合成酶之相關研究及文獻,若能成功的將基因選殖出來並鑑定出功能,除了可與其他植物的揮發性萜類合成酶進行比較外,亦能作為未來為重要成分的半化學合成之設計藍圖。本次實驗自臺灣扁柏的枝條進行轉錄體的次世代定序,並從資料庫中獲得 6 條揮發性萜類合成酶的序列,分別為 Covf_121、Covf_339、Covf_13139、Covf_2194、Covf_16187 及 Covf_10716,並透過 in vitro 及 in vivo 實驗之結果與胺基酸中保守功能序列的分析,可將 6 條揮發性萜類合成酶更進一步區分成單萜及倍半萜合成酶,並且依主要產物對其命名,單萜合成酶分別為 CovfCar (3-Carene synthase)、CovfLin (Linalool synthase) 及 CovfTer (Terpinolene synthase),以及倍半萜類合成酶為 CovfGerA (Germacrene A synthase)、CovfAco (Acoradiene synthase) 與 CovfLon (Longifolene synthase)。 | zh_TW |
dc.description.abstract | Chamaecyparis obtusa var. formosana, variant of Chamaecyparis obtusa, is a precious and endemic plant in Taiwan. It is very widely used. The trunk with good texture and color is suitable for building materials and furniture, and the whole plant is rich in aroma, which can be made into essential oils, fragrance and a series of related products. Volatile terpenoids are the major compounds in the composition of essential oils, many of which can only be found in C. obtusa var. formosana. Further research on their synthases was carried out to better understand the biosynthesis mechanism of these products. However, there were no reports on terpenoid synthases in C. obtusa var. formosana until now. Thus, we cloned and identified their functions to obtain the information for understanding the relationship between other plant volatile-terpenoid synthases and serving as a blueprint for the semi-chemical synthesis of important components in the future. In this experiment, the sequences of 6 volatile-terpenoid synthases were obtained from the Next Generation Sequence (NGS) database of branch transcripts of C. obtusa var. formosana, including Covf_121, Covf_339, Covf_13139, Covf_2194, Covf_16187 and Covf_10716. Through the results of in vitro and in vivo experiments and the analysis of conserved motif sequences in amino acids, the six volatile terpenoid synthases can be further distinguished into monoterpenoid synthases and sesquiterpenoid synthases, named according to their main products. There are CovfCar (3-Carene synthase), CovfLin (Linalool synthase) and CovfTer (Terpinolene synthase) of monoterpenoid synthases and CovfGerA (Germacrene A synthase), CovfAco (Acoradiene synthase) and CovfLon (Longifolene synthase) of sesquiterpenoid synthases. | en |
dc.description.provenance | Made available in DSpace on 2023-03-19T21:11:13Z (GMT). No. of bitstreams: 1 U0001-2508202215382600.pdf: 14928597 bytes, checksum: ca5aca7a82e2cc86641aa3a4e85b3927 (MD5) Previous issue date: 2022 | en |
dc.description.tableofcontents | 謝誌 i 摘要 iii Abstract iv 目錄 iv 表目錄 ix 圖目錄 xi 第一章、前言 1 第二章、文獻回顧 4 2.1 植物體中的有機揮發性成分 4 2.2 萜類化合物介紹 6 2.2.1 萜類化合物 6 2.2.2 萜類化合物的生合成途徑 8 2.3 萜類合成酶概述 11 2.3.1 萜類合成酶的基因型態 11 2.3.2 萜類合成酶的結構特徵 12 2.3.3萜類合成酶受質選擇性 15 2.3.4 萜類合成酶的演化分群 18 2.3.5 萜類合成酶的內含子分布情況 22 2.4 常見的單萜類化合物之生合成機制 24 2.4.1 線性單萜類化合物之生合成機制 24 2.4.2 環狀單萜類化合物之生合成機制 26 2.5 常見的倍半萜類化合物之生合成機制 28 2.5.1 線性倍半萜類化合物之生合成機制 28 2.5.2 環狀倍半萜類化合物之生合成機制 29 2.5.3 多環結構倍半萜類化合物之生合成機制 31 第三章、材料與方法 33 3.1 實驗試材 33 3.1.1 植物材料 33 3.1.2 轉殖載體 34 3.1.3 菌株之挑選 35 3.1.4 藥劑配製 35 3.2 臺灣扁柏Total RNA萃取 37 3.3 臺灣扁柏枝條皮部轉錄體資料庫 38 3.4 臺灣扁柏枝條之揮發性萜類合成酶基因選殖 39 3.4.1 篩選目標基因 39 3.4.2 基因選殖 40 3.5 點突變 (Site directed mutagenesis) 試驗 43 3.6 色素體運輸肽及蛋白質分子量 (MW) 與等電點 (pI) 之預測 44 3.7 蛋白質表現質體之構築 (Construction) 45 3.7.1 接合反應 (Ligation) 45 3.7.2 浸入反應 (Infusion) 45 3.8 西方墨點試驗 (Western blot) 46 3.9 重組蛋白質純化 48 3.9.1 液態培養 48 3.9.2 蛋白質純化 48 3.9.3 SDS-PAGE 電泳及純化之目標蛋白濃度測定 49 3.10 重組蛋白質活性反應 49 3.11 大腸桿菌in vivo Co-expression反應系統 51 3.11.1 小量培養 (50 mL) 51 3.11.2 大量培養 (500 mL) 52 3.12 氣相層析質譜儀分析產物 52 3.12.1 氣相層析質譜儀 52 3.12.2 單萜及倍半萜類之固相微萃取試驗及自動進樣分析條件 53 3.12.3 雙萜類之自動進分析機條件 53 3.12.4 Germacrene A 之固相微萃取試驗及自動進樣分析條件 54 3.12.4 Germacrene A 之固相微萃取試驗及自動進樣分析條件 (低注射口溫度) 54 3.12.5 產物分析 55 3.13 以固相微萃取方式分析臺灣扁柏揮發性成分 56 3.14 蛋白質結構模擬 56 3.15 產物之分離與純化 56 3.16 核磁共振儀解析倍半萜化合物之結構 57 3.17 臺灣扁柏萜類合成酶基因之內含子與外顯子的分布 58 3.18 裸子植物萜類合成酶親源關係樹狀圖 (Phylogenetic tree) 分析 59 第四章、結果 60 4.1 臺灣扁柏葉子及枝條之揮發性成分分析 60 4.2 萜類合成酶選殖資訊 65 4.3 點突變結果 71 4.3.1 Covf_10716 移碼突變情況 71 4.3.2 以點突變之方式創造 1 條完整的 Covf_10716 序列 74 4.3.3 從日本扁柏基因中釣取相似之萜類合成酶 75 4.3.4 萜類合成酶之基因在不同株臺灣扁柏的表現結果 77 4.3.5 Covf_10716序列探討之流程圖 79 4.4 萜類合成酶表現質體之構築與西方墨點試驗之結果 80 4.4.1 質體構築 80 4.4.2 西方墨點試驗分析 81 4.5 純化萜類合成酶 in vitro 反應 92 4.5.1 純化重組蛋白之濃度與 SDS-PAGE 圖 92 4.5.2 純化重組蛋白與前驅物進行 in vitro 反應之 GC-MS 分析圖 96 4.6 萜類合成酶 in vivo 反應 111 4.6.1 臺灣扁柏萜類合成酶以 GPP synthase 進行 in vivo 反應 114 4.6.2 臺灣扁柏萜類合成酶以 FPP synthase 進行 in vivo 反應 120 4.6.3 日本扁柏萜類合成酶以 FPP synthase 進行 in vivo 反應 127 4.6.4 臺灣杉萜類合成酶以 FPP synthase 進行 in vivo 反應 132 4.7 Covf 16187 之生合成產物的分離與純化 133 4.7.1 透過逆相高壓液相層析儀進行分離與純化 133 4.7.2 Covf_16187純化後各分離部之 GC-MS 結果分析圖 134 4.8 Covf 16187 各分離部 NMR 分析結果 135 4.8.1 Covf_16187 fraction 4 (Unknown 2) 135 4.8.2 Covf_16187 fraction 5 (Unknown 4) 140 4.8.3 Covf_16187 fraction 6 (Unknown 1 & Unknown 3) 145 4.9 臺灣杉 TcTPS2 產物 NMR 分析結果 151 4.10 臺灣扁柏與日本扁柏之萜類合成酶的命名依據 154 4.11 裸子植物揮發性萜類合成酶之親緣關係樹 155 4.12 臺灣扁柏萜類合成酶之蛋白質結構分析 157 4.13 萜類合成酶內含子分布情況 159 第五章、討論 161 5.1 萜類合成酶序列之分析及重要功能之保守 Motif 161 5.2 萜類合成酶之序列完整性 163 5.3 萜類合成酶產物分析 165 5.3.1 CovfCar 單萜類合成酶 165 5.3.2 CovfLin 單萜類合成酶 167 5.3.3 CovfTer 單萜類合成酶 168 5.3.4 CovfGerA 倍半萜類合成酶 170 5.3.5 CovfAco 倍半萜類合成酶 171 5.3.6 CovfLon 倍半萜類合成酶 174 5.3.7 Covf 倍半萜類合成酶 in vitro 實驗結果之分析 176 5.4 萜類合成酶之親緣關係樹分群 178 第六章、結論 181 第七章、參考文獻 182 附錄 193 | - |
dc.language.iso | zh_TW | - |
dc.title | 臺灣扁柏枝條之揮發性萜類合成酶基因選殖與功能鑑定 | zh_TW |
dc.title | Gene Cloning and Functional Characterization of Volatile-terpene Synthase Gene from Branches of Chamaecyparis obtusa var. formosana | en |
dc.type | Thesis | - |
dc.date.schoolyear | 110-2 | - |
dc.description.degree | 碩士 | - |
dc.contributor.oralexamcommittee | 王升陽;孫英玄;何振隆;吳家禎 | zh_TW |
dc.contributor.oralexamcommittee | Sheng-Yang Wang;Ying-Hsuan Sun;Chen-Lung Ho;Chia-Chen Wu | en |
dc.subject.keyword | 臺灣扁柏,次世代定序,萜類合成酶,單萜類化合物,倍半萜類化合物, | zh_TW |
dc.subject.keyword | Chamaecyparis obtusa var. formosana,Next Generation Sequence (NGS),Terpene synthase,Monoterpeniods,Sesquiterpenoids, | en |
dc.relation.page | 197 | - |
dc.identifier.doi | 10.6342/NTU202202813 | - |
dc.rights.note | 未授權 | - |
dc.date.accepted | 2022-08-29 | - |
dc.contributor.author-college | 生物資源暨農學院 | - |
dc.contributor.author-dept | 森林環境暨資源學系 | - |
顯示於系所單位: | 森林環境暨資源學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-110-2.pdf 目前未授權公開取用 | 14.58 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。